Affinage

DBT

Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial · UniProt P11182

Round 2 corrected
Length
482 aa
Mass
53.5 kDa
Annotated
2026-04-28
98 papers in source corpus 16 papers cited in narrative 16 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DBT encodes the dihydrolipoamide branched-chain transacylase (E2) core subunit of the mitochondrial branched-chain α-keto acid dehydrogenase (BCKDH) complex, which catalyzes an essential step in branched-chain amino acid catabolism; its 24-meric cubic core structure is required for complex assembly, and truncating mutations cause rapid E2 degradation and loss of BCKDH activity, resulting in maple syrup urine disease (MSUD) (PMID:8430702, PMID:35799415). Beyond its canonical metabolic role, the DBT lipoyl-binding domain engages ANXA2 to activate Hippo/YAP signaling and suppress lipogenesis in clear-cell renal cell carcinoma, functioning as a tumor suppressor (PMID:36860124). Loss of DBT shifts cellular energy status to activate AMPK-dependent autophagy, compensating for proteasomal impairment and protecting against TDP-43 proteotoxicity in ALS models across Drosophila and mammalian neurons (PMID:39255192). The lipoyl domain is also the principal conformational epitope recognized by autoantibodies in primary biliary cholangitis (PMID:7543435, PMID:39769438).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 1991 High

    Chromosomal mapping of DBT to 1p31 established its genomic context and enabled subsequent mutation screening in MSUD families.

    Evidence Somatic cell hybrid analysis and in situ hybridization in human cells

    PMID:1889817

    Open questions at the time
    • No functional characterization performed at this stage
    • Regulatory elements not defined
  2. 1993 High

    Demonstration that truncating DBT mutations prevent assembly of the 24-mer E2 cubic core and cause rapid protein degradation established the structural basis of MSUD pathogenesis.

    Evidence Transfection of mutant E2 constructs into E2-deficient MSUD cell line with assembly and degradation assays

    PMID:8430702

    Open questions at the time
    • Only two mutations characterized; spectrum of pathogenic variants not surveyed
    • No rescue experiment with wild-type cDNA reported
  3. 1995 High

    Mapping of the conformational autoantibody epitope to the N-terminal lipoyl-binding region (aa 1–227) of DBT linked this domain to autoimmune recognition in primary biliary cirrhosis, independent of lipoic acid attachment.

    Evidence ELISA and immunoblot absorption with recombinant domain fragments against PBC patient sera

    PMID:7543435

    Open questions at the time
    • Mechanism by which tolerance to this epitope is broken remains unknown
    • No structural resolution of the antibody–antigen interface
  4. 2015 Medium

    Identification of a hypoxia-enhanced interaction between peroxiredoxin V and DBT, with increased DBT enzymatic activity under hypoxia, suggested redox-dependent regulation of BCKDH activity.

    Evidence Immunoprecipitation–mass spectrometry interactomics in mouse kidney under normoxia versus hypoxia, enzymatic activity assay

    PMID:25670924

    Open questions at the time
    • Functional consequence of the PrdxV–DBT interaction on BCAA catabolism not established
    • Interaction not validated by reciprocal pulldown or in additional tissues
    • Mechanism linking Cys48 of PrdxV to DBT activity unclear
  5. 2022 Medium

    Patient-derived hepatocyte studies confirmed that complete loss or reduction of DBT protein abolishes BCKDH complex activity and causes accumulation of branched-chain amino acids and α-ketoacids, directly linking genotype to metabolic phenotype at the cellular level.

    Evidence Western blot of patient liver cells with homozygous exon 2 deletion and compound heterozygous mutations, UPLC-MS/MS metabolite profiling

    PMID:35799415

    Open questions at the time
    • No BCKDH complex assembly assay performed on these specific alleles
    • Residual E2 protein from the Ser306Pro allele not functionally characterized in isolation
  6. 2023 Medium

    Discovery that the DBT lipoyl-binding domain interacts with ANXA2 to activate Hippo signaling and repress YAP-driven lipogenic transcription revealed a non-canonical tumor-suppressive role for DBT in ccRCC, with its downregulation mediated by METTL3-dependent m6A modification.

    Evidence Co-IP with lipoyl domain mutants, mass spectrometry, luciferase reporters, and gain/loss-of-function assays in ccRCC models in vitro and in vivo

    PMID:36860124

    Open questions at the time
    • Single-lab finding; independent replication needed
    • Structural basis of lipoyl domain–ANXA2 interaction unresolved
    • Extent of this mechanism in other cancer types unknown
  7. 2024 High

    A genome-wide CRISPR screen revealed that DBT loss activates AMPK-dependent autophagy to compensate for proteasomal impairment, protecting against proteotoxicity from ALS-linked mutant TDP-43 across species, repositioning DBT as a metabolic switch governing protein quality control.

    Evidence CRISPR screen in mammalian cells, genetic loss-of-function in Drosophila and mammalian neurons, AMPK pathway and ubiquitinated protein clearance assays

    PMID:39255192

    Open questions at the time
    • Precise metabolite(s) linking DBT loss to AMPK activation not identified
    • Whether this protective mechanism operates in human ALS patients is untested
    • Potential detrimental effects of chronic DBT loss on BCAA metabolism not addressed
  8. 2024 Medium

    Fine-mapping of the autoantibody epitope in PBC to specific residues (Glu4, Ile13) in the lipoyl β-sheet of DBT, with structural validation by EPR spectroscopy, refined understanding of conformational determinants of autoimmune recognition.

    Evidence Site-directed mutagenesis of lipoyl domain, ELISA with PBC patient sera, MTSSL spin-labeling and EPR spectroscopy

    PMID:39769438

    Open questions at the time
    • Crystal structure of the autoantibody–lipoyl domain complex not solved
    • Whether these residues drive pathogenic T-cell responses is unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • The metabolic signals linking DBT loss to AMPK activation and the structural basis of DBT's interaction with ANXA2 in Hippo pathway regulation remain undefined, as does whether the proteotoxicity-protective mechanism of DBT loss can be therapeutically exploited without disrupting BCAA homeostasis.
  • No structural model of full-length human DBT in complex context
  • Metabolite mediator of DBT loss → AMPK axis not identified
  • Therapeutic window for DBT modulation unexplored

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 2
Localization
GO:0005739 mitochondrion 2
Pathway
R-HSA-1643685 Disease 4 R-HSA-1430728 Metabolism 3 R-HSA-162582 Signal Transduction 1 R-HSA-9612973 Autophagy 1
Partners
ANXA2PRDX5
Complex memberships
BCKDH complex (branched-chain α-keto acid dehydrogenase complex)

Evidence

Reading pass · 16 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 Drosophila DBT (doubletime, CKIε ortholog) directly phosphorylates and destabilizes the circadian transcription factor CLOCK (CLK) in a phase-specific manner in vivo, promoting CLK hyperphosphorylation and enhancing its degradation; DBT also evokes modest inhibition of CLK-dependent transactivation. DBT functions in dynamic equilibrium with protein phosphatase 2A to maintain balanced hypo- and hyperphosphorylated CLK isoforms, stabilizing CLK levels against stochastic fluctuations. In vivo genetic analysis in Drosophila, Drosophila S2 cell transfection assays, immunostaining for subcellular localization of CLK Proceedings of the National Academy of Sciences of the United States of America High 16603629
2007 Enzymatically active DBT kinase activity is required for normal circadian rhythms in Drosophila. A kinase-dead DBT(K38R) mutant interacts with PER but cannot phosphorylate it; expression of DBT(K38R) in flies antagonizes PER phosphorylation and degradation, dampens PER oscillation, and produces long circadian periods or arrhythmicity in proportion to titration of endogenous DBT activity. Site-directed mutagenesis (K38R) abolishing kinase activity, in vitro kinase assay, S2 cell transfection, transgenic fly behavioral analysis Molecular and cellular biology High 17893330
2008 DBT directly phosphorylates the Drosophila clock protein PER at specific sites. DBT-dependent phosphorylation at two adjoining sequence motifs acts in a switch-like manner to regulate PER stability and repressor function. The per(S) short-period mutation alters a key DBT phosphorylation target within PER, linking phosphorylation site identity to period length. In vitro kinase assay with purified proteins, phosphorylation site mapping by mutagenesis, S2 cell functional assays, in vivo behavioral analysis PLoS biology High 18666831
2007 PER, TIM, and DBT form physical interactions in Drosophila cells; live-cell imaging revealed dynamic patterns of DBT, PER, and TIM stability and localization consistent with a cytoplasmic interval timer that regulates nuclear translocation of PER and TIM once per day. Live-cell video microscopy with GFP-tagged proteins in cultured Drosophila cells, co-immunoprecipitation Cold Spring Harbor symposia on quantitative biology Medium 18419263
2013 The non-canonical FK506-binding protein BRIDE OF DOUBLETIME (BDBT/CG17282) physically interacts with DBT kinase. BDBT enhances DBT-dependent PER degradation in S2 cells; RNAi knockdown of BDBT produces behavioral arrhythmicity and elevated hypophosphorylated nuclear PER and phosphorylated DBT. Structural analysis showed BDBT contains an inactive peptide prolyl-isomerase domain that binds DBT and tetratricopeptide repeats that may scaffold larger complexes. Proteomic identification of DBT interactors, reciprocal co-IP, RNAi knockdown with behavioral and molecular phenotyping, S2 cell overexpression assays, structural analysis Neuron High 24210908
2015 Drosophila DBT undergoes C-terminal autophosphorylation at six serine/threonine residues identified by mass spectrometry. Mutation of these sites (DBT(C/ala)) prevents autophosphorylation-dependent DBT turnover in S2 cells. Unlike vertebrate CKIδ, DBT autophosphorylation does not reduce in vitro kinase activity and is not required for circadian clock function. However, DBT autophosphorylation is required for protection against UV-induced apoptosis: wild-type DBT protects S2 cells and larvae from UV-induced apoptosis and is degraded by the proteasome after UV exposure, while DBT(C/ala) does not protect. The HSP90 co-chaperone SPAG antagonizes DBT autophosphorylation in S2 cells. Mass spectrometry for phosphosite mapping, site-directed mutagenesis, in vitro kinase assay, S2 cell UV apoptosis assay, proteasome inhibitor studies, transgenic fly behavioral analysis Molecular and cellular biology High 25939385
2022 DBT (BDBT) foci in Drosophila eye photoreceptors require both CRYPTOCHROME and RHODOPSIN-1 visual photoreceptor pathways for light-induced disappearance; arrestin mutants (arr1, arr2) that affect rhodopsin quenching eliminated BDBT foci in darkness and caused increased nuclear PER. Knockdown of BDBT specifically in the eye produced constitutively nuclear PER and constitutively cytosolic DBT, demonstrating that BDBT is required for co-transport of DBT and PER into the nucleus. Genetic analysis with circadian and visual pathway mutants, tissue-specific RNAi knockdown, immunofluorescence localization iScience Medium 36994075
2022 Reduction of DBT kinase in Drosophila circadian neurons increases sleep loss at night (via adult clock function), while reduction in non-circadian neurons produces increased daytime sleep (via developmental effects). PER protein is involved in DBT-mediated sleep regulation independently of the neuronal context. CRISPR-Cas9 knock-in of GAL4 at dbt locus, targeted RNAi in neuron subsets, sleep behavioral assays PLoS genetics Medium 35139068
1993 Two novel loss-of-function mutations in the human DBT (E2) gene cause MSUD: a 2-bp (AT) deletion in exon 2 causing a frameshift in the mitochondrial targeting presequence, and a G-to-T nonsense mutation in exon 6 (E163*). Transfection of the E163* construct into an E2-deficient cell line showed that the truncated E2 protein cannot assemble into the native 24-mer cubic structure and is rapidly degraded, establishing the structural requirement for the cubic core in DBT protein stability. Site-directed mutation, transfection into E2-deficient MSUD cell line, protein assembly assay, degradation analysis American journal of human genetics High 8430702
1995 Autoantibodies to BCOADC-E2 (DBT) in primary biliary cirrhosis recognize a conformational epitope located within amino acids 1–227 of the mature protein that includes the lipoic acid-binding region. Full-length protein (aa 1–421) is required to absorb all detectable anti-BCOADC-E2 reactivity. Antibody binding is not dependent on the presence of lipoic acid on the antigen. Immunoblotting, ELISA, and selective absorption of patient sera with defined recombinant protein fragments spanning the full DBT/BCOADC-E2 sequence Hepatology (Baltimore, Md.) High 7543435
1991 The human DBT gene (encoding the E2/transacylase subunit of the BCKDH complex) was localized to chromosome 1 by somatic cell hybrid analysis and regionally assigned to chromosome band 1p31 by in situ hybridization. Human × mouse somatic cell hybrid panel analysis with PCR-amplified E2 cDNA probe, in situ hybridization Genomics High 1889817
2015 Peroxiredoxin V (PrdxV) interacts with DBT (dihydrolipoamide branched chain transacylase E2) in mouse kidney under hypoxic stress. This interaction is enhanced under hypoxia compared to normoxia. Hypoxic conditions also increase DBT enzymatic activity. The peroxidatic cysteine residue (Cys48) of PrdxV is responsible for the interaction with DBT. Enhanced colocalization of PrdxV and DBT was observed in vitro under hypoxic conditions. Immunoprecipitation coupled with nano-UPLC-MS shotgun proteomics, comparative interactomics under normoxia vs. hypoxia, co-localization imaging, enzymatic activity assay Proteome science Medium 25670924
2023 DBT (dihydrolipoamide branched chain transacylase E2) interacts with ANXA2 (annexin A2) through its lipoyl-binding domain to activate Hippo signaling, resulting in decreased nuclear localization of YAP and transcriptional repression of lipogenic genes. DBT downregulation in ccRCC is caused by METTL3-mediated m6A modification. DBT acts as a tumor suppressor by inhibiting tumor progression and correcting lipid metabolism disorder in ccRCC. Luciferase reporter assay, immunoprecipitation, mass spectrometry, mutational studies of the lipoyl-binding domain, gain/loss-of-function assays in vitro and in vivo Cancer communications (London, England) Medium 36860124
2024 Loss of DBT (dihydrolipoamide branched chain transacylase E2) protects against proteasome inhibition-associated cell death by promoting clearance of ubiquitinated proteins. Loss of DBT alters cellular metabolic and energetic status, activating autophagy through an AMPK-dependent mechanism when the proteasome is inhibited. DBT loss protects against proteotoxicity from ALS-linked mutant TDP-43 in both Drosophila and mammalian neurons. DBT is upregulated in ALS patient tissues, positioning it as a metabolic switch controlling protein quality control pathways. Genome-wide CRISPR screen, genetic loss-of-function in Drosophila and mammalian neurons, AMPK pathway activation assays, ubiquitinated protein clearance assays eLife High 39255192
2024 The lipoyl domain of BCOADC-E2 (DBT) is critical for autoantibody recognition in primary biliary cholangitis. The folding conformation of the lipoyl β-sheet, specifically the glutamic acid at position 4 and isoleucine at position 13, are necessary for recognition by anti-BCOADC-E2 autoantibodies. Multi-site mutations of these residues reduce reactivity with patient sera, and EPR spectroscopy confirmed the conformational integrity of these positions is maintained in the wild-type protein. Site-directed mutagenesis of lipoyl domain, ELISA with patient sera, MTSSL spin-labeling and EPR spectroscopy International journal of molecular sciences Medium 39769438
2022 Homozygous deletion of exon 2 (delEx2) of the DBT gene results in complete absence of DBT protein in hepatocytes (confirmed by Western blot), while compound heterozygous delEx2/p.Ser306Pro produces decreased DBT protein. Both variants cause failure of branched-chain amino acid catabolism, with accumulation of branched-chain amino acids and alpha-ketoacids in explanted hepatocytes demonstrated by UPLC-MS/MS, establishing the functional requirement of the DBT E2 subunit for BCKDH complex activity. Western blot of patient-derived liver cells, UPLC-tandem mass spectrometry of hepatocyte metabolites, Sanger sequencing American journal of medical genetics. Part A Medium 35799415

Source papers

Stage 0 corpus · 98 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2004 A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nature cell biology 841 14743216
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2015 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. Cell 433 26638075
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2011 IFIT1 is an antiviral protein that recognizes 5'-triphosphate RNA. Nature immunology 405 21642987
2007 The layered structure of human mitochondrial DNA nucleoids. The Journal of biological chemistry 340 18063578
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2016 Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell reports 306 27342126
2009 Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. Cell 281 19167051
2011 WWP2 is an E3 ubiquitin ligase for PTEN. Nature cell biology 266 21532586
2022 EWSR1-induced circNEIL3 promotes glioma progression and exosome-mediated macrophage immunosuppressive polarization via stabilizing IGF2BP3. Molecular cancer 257 35031058
2021 Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context. Cell metabolism 239 34800366
2016 Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function. Molecular cell 220 27499296
2014 Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. Journal of proteomics 215 25281560
2020 A High-Density Human Mitochondrial Proximity Interaction Network. Cell metabolism 148 32877691
2017 Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation. Nucleic acids research 148 28973437
2019 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nature cell biology 137 31871319
2022 Human transcription factor protein interaction networks. Nature communications 123 35140242
2017 The human cytoplasmic dynein interactome reveals novel activators of motility. eLife 118 28718761
2007 Toward a confocal subcellular atlas of the human proteome. Molecular & cellular proteomics : MCP 114 18029348
2006 Balance between DBT/CKIepsilon kinase and protein phosphatase activities regulate phosphorylation and stability of Drosophila CLOCK protein. Proceedings of the National Academy of Sciences of the United States of America 107 16603629
1995 Autoantibodies to BCOADC-E2 in patients with primary biliary cirrhosis recognize a conformational epitope. Hepatology (Baltimore, Md.) 97 7543435
2007 Drosophila DBT lacking protein kinase activity produces long-period and arrhythmic circadian behavioral and molecular rhythms. Molecular and cellular biology 75 17893330
2008 Activating PER repressor through a DBT-directed phosphorylation switch. PLoS biology 70 18666831
1976 Mouse hepatitis virus (MHV-2). Plaque assay and propagation in mouse cell line DBT cells. Japanese journal of microbiology 69 184329
2003 Practice-based outcomes of dialectical behaviour therapy (DBT) targeting anger and violence, with male forensic patients: a pragmatic and non-contemporaneous comparison. Criminal behaviour and mental health : CBMH 52 14654871
1993 Occurrence of a 2-bp (AT) deletion allele and a nonsense (G-to-T) mutant allele at the E2 (DBT) locus of six patients with maple syrup urine disease: multiple-exon skipping as a secondary effect of the mutations. American journal of human genetics 47 8430702
2023 N6-methyladenosine-modified DBT alleviates lipid accumulation and inhibits tumor progression in clear cell renal cell carcinoma through the ANXA2/YAP axis-regulated Hippo pathway. Cancer communications (London, England) 46 36860124
2017 PSFM-DBT: Identifying DNA-Binding Proteins by Combing Position Specific Frequency Matrix and Distance-Bigram Transformation. International journal of molecular sciences 44 28841194
2017 PET imaging of α7 nicotinic acetylcholine receptors: a comparative study of [18F]ASEM and [18F]DBT-10 in nonhuman primates, and further evaluation of [18F]ASEM in humans. European journal of nuclear medicine and molecular imaging 43 28120003
1999 Purification and characterization of dibenzothiophene (DBT) sulfone monooxygenase, an enzyme involved in DBT desulfurization, from Rhodococcus erythropolis D-1. Journal of bioscience and bioengineering 35 16232672
2006 Biodesulfurization of DBT in tetradecane and crude oil by a facultative thermophilic bacterium Mycobacterium goodii X7B. Journal of biotechnology 29 16905217
1981 Persistent infection with mouse hepatitis virus, JHM strain in DBT cell culture. Advances in experimental medicine and biology 29 6278888
2010 Tributyltin (TBT) and dibutyltin (DBT) differently inhibit the mitochondrial Mg-ATPase activity in mussel digestive gland. Toxicology in vitro : an international journal published in association with BIBRA 26 20950683
2011 Desulfurization of dibenzothiophene (DBT) by a novel strain Lysinibacillus sphaericus DMT-7 isolated from diesel contaminated soil. Journal of environmental sciences (China) 25 22066220
2013 In vitro approaches to evaluate toxicity induced by organotin compounds tributyltin (TBT), dibutyltin (DBT), and monobutyltin (MBT) in neuroblastoma cells. Journal of agricultural and food chemistry 24 23534342
2002 Recombinant Rhodococcus sp. strain T09 can desulfurize DBT in the presence of inorganic sulfate. Current microbiology 23 12192519
2020 A randomized controlled trial comparing the clinical efficacy and cost-effectiveness of eye movement desensitization and reprocessing (EMDR) and integrated EMDR-Dialectical Behavioural Therapy (DBT) in the treatment of patients with post-traumatic stress disorder and comorbid (Sub)clinical borderline personality disorder: study design. BMC psychiatry 22 32762677
1999 In situ nucleic acid detection of PDC-E2, BCOADC-E2, OGDC-E2, PDC-E1alpha, BCOADC-E1alpha, OGDC-E1, and the E3 binding protein (protein X) in primary biliary cirrhosis. Hepatology (Baltimore, Md.) 22 10385636
2013 Noncanonical FK506-binding protein BDBT binds DBT to enhance its circadian function and forms foci at night. Neuron 21 24210908
2018 Fourteen new mutations of BCKDHA, BCKDHB and DBT genes associated with maple syrup urine disease (MSUD) in Malaysian population. Molecular genetics and metabolism reports 18 30228974
2016 CBT/DBT skills training for adults with attention deficit hyperactivity disorder (ADHD). Psychiatria Danubina 17 27663817
2015 Eleven novel mutations of the BCKDHA, BCKDHB and DBT genes associated with maple syrup urine disease in the Chinese population: Report on eight cases. European journal of medical genetics 17 26453840
2007 A PER/TIM/DBT interval timer for Drosophila's circadian clock. Cold Spring Harbor symposia on quantitative biology 17 18419263
2017 Twenty novel mutations in BCKDHA, BCKDHB and DBT genes in a cohort of 52 Saudi Arabian patients with maple syrup urine disease. Molecular genetics and metabolism reports 16 28417071
2015 PET imaging evaluation of [(18)F]DBT-10, a novel radioligand specific to α7 nicotinic acetylcholine receptors, in nonhuman primates. European journal of nuclear medicine and molecular imaging 16 26455500
1999 Evidence for a separate genetic origin of the partial D phenotype DBT in a Japanese family. Transfusion 16 10604255
1978 Physico-chemical properties of mouse hepatitis virus (MHV-2) grown on DBT cell culture. Microbiology and immunology 16 30881
2015 Interaction of peroxiredoxin V with dihydrolipoamide branched chain transacylase E2 (DBT) in mouse kidney under hypoxia. Proteome science 15 25670924
1991 Regional assignment of two genes of the human branched-chain alpha-keto acid dehydrogenase complex: the E1 beta gene (BCKDHB) to chromosome 6p21-22 and the E2 gene (DBT) to chromosome 1p31. Genomics 15 1889817
2003 Maple syrup urine disease in the Austronesian aboriginal tribe Paiwan of Taiwan: a novel DBT (E2) gene 4.7 kb founder deletion caused by a nonhomologous recombination between LINE-1 and Alu and the carrier-frequency determination. European journal of human genetics : EJHG 13 14508502
2018 Impact on mindfulness, emotion regulation, and emotional overeating of a DBT skills training group: a pilot study. Eating and weight disorders : EWD 12 30443799
2016 DBT desulfurization by decorating Rhodococcus erythropolis IGTS8 using magnetic Fe3O4 nanoparticles in a bioreactor. Engineering in life sciences 12 32624797
2010 CYP1A expression in liver and gills of rainbow trout (Oncorhynchus mykiss) after short-term exposure to dibenzothiophene (DBT). Chemosphere 9 20167346
1995 Characterization of DBT cell clones derived from cells persistently infected with the JHM strain of mouse hepatitis virus. The Journal of veterinary medical science 8 8593285
2018 Four novel mutations of the BCKDHA, BCKDHB and DBT genes in Iranian patients with maple syrup urine disease. Journal of pediatric endocrinology & metabolism : JPEM 6 29306928
1999 Detection of anti-branched chain 2-oxo acid dehydrogenase complex (BCOADC)-E2 antibody in primary biliary cirrhosis by ELISA using recombinant fusion protein. Autoimmunity 6 10433090
2024 Underlying mechanisms of novel cuproptosis-related dihydrolipoamide branched-chain transacylase E2 (DBT) signature in sunitinib-resistant clear-cell renal cell carcinoma. Aging 5 38305803
2022 Genomic and biochemical analysis of repeatedly observed variants in DBT in individuals with maple syrup urine disease of Central American ancestry. American journal of medical genetics. Part A 5 35799415
2014 DBT- and DBTO2-induced dysplasia and their associated proteomic alterations in the small intestines of Wistar rats. Journal of proteome research 5 25369245
2012 Oxidation of dibenzothiophene (DBT) by Serratia marcescens UCP 1549 formed biphenyl as final product. Biotechnology for biofuels 5 22583489
2000 Identification of the gene encoding a NAD(P)H-flavin oxidoreductase coupling with dibenzothiophene (DBT)-desulfurizing enzymes from the DBT-nondesulfurizing bacterium Paenibacillus polymyxa A-1. Journal of bioscience and bioengineering 5 16232847
2022 Pathogenic Homozygous Mutations in the DBT Gene (c.1174A>C) Result in Maple Syrup Urine Disease in a rs12021720 Carrier. Laboratory medicine 4 35657820
2021 Dibutyltin (DBT) inhibits in vitro androgen biosynthesis of rat immature Leydig cells. Toxicology 4 33862173
2007 Dibenzothiophene oxidation by horseradish peroxidase in organic media: effect of the DBT:H2O2 molar ratio and H2O2 addition mode. Chemosphere 4 18022671
1991 Localization of the dihydrolipoamide branched-chain transacylase gene (DBT) of the human branched-chain keto acid dehydrogenase complex to chromosome 1. Cytogenetics and cell genetics 4 2004553
1982 Heterologous response of antiserum-treated cell clones from a persistently infected DBT cell line to mouse hepatitis virus. The Japanese journal of experimental medicine 4 6302351
2023 Visual and circadian regulation of Drosophila BDBT and BDBT effects on DBT and PER localization. iScience 3 36994075
2022 DBT affects sleep in both circadian and non-circadian neurons. PLoS genetics 3 35139068
2022 Impaired fasting glucose, oxidative distress, and cognitive impairment. Is this the starting point on DBT cognitive decline? Frontiers in aging neuroscience 3 35959297
2019 Case report: maple syrup urine disease with a novel DBT gene mutation. BMC pediatrics 3 31830945
2015 Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis. Molecular and cellular biology 3 25939385
2013 Identification of aldolase and ferredoxin reductase within the dbt operon of Burkholderia fungorum DBT1. Journal of basic microbiology 3 23686744
2025 Comparative analysis of biodesulfurization of dibenzothiophene (DBT) and 4,6-dimethyl dibenzothiophene (4,6-DMDBT) by 4S pathway using molecular simulations. Preparative biochemistry & biotechnology 2 39748703
2024 DBT is a metabolic switch for maintenance of proteostasis under proteasomal impairment. eLife 2 39255192
1998 Expression of a recombinant branched chain alpha-oxo acid dehydrogenase complex E2 (BCOADC-E2) in insect cells and its immunoreactivity to autoimmune sera. Experimental & molecular medicine 2 9873825
2025 Boosting Dibenzothiophene Biodesulfurization Through Implantation of a Refactored DBT Pathway in a Tailored Pseudomonas putida Chassis. Microbial biotechnology 1 40932091
2024 DBT is a metabolic switch for maintenance of proteostasis under proteasomal impairment. bioRxiv : the preprint server for biology 1 37745492
2024 Probing the Effects of Multisite Mutations in the Lipoic Acid Region of the BCOADC-E2 Protein. International journal of molecular sciences 1 39769438
2021 Three novel mutations of the BCKDHA, BCKDHB and DBT genes in Chinese children with maple syrup urine disease. Journal of pediatric endocrinology & metabolism : JPEM 1 34883003
2019 The Gas-Phase Formation Mechanism of Dibenzofuran (DBF), Dibenzothiophene (DBT), and Carbazole (CA) from Benzofuran (BF), Benzothiophene (BT), and Indole (IN) with Cyclopentadienyl Radical. International journal of molecular sciences 1 31683506
2026 DBT-K for Adolescents: Feasibility and Preliminary Outcomes of a Creative Eight-Week, DBT-Based Transdiagnostic Skills Group. Children (Basel, Switzerland) 0 41749528
2026 Enhancing the Clinical Feasibility of Morita Therapy in China Through the Integration of ACT and DBT: A Theoretical Framework and Implementation Protocol. Journal of evaluation in clinical practice 0 41802175
2025 Alloanti-D induction in a rare RhD variant (DBT-2) case: Insights from serological and molecular biological testing. Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis 0 39908659
2025 Exogenous DBT and overexpression of deoR improved the waste tire rubber desulfurization ability of a newly isolated Escherichia sp. strain. Journal of hazardous materials 0 41086491
2025 The mediating role of social connectedness between inflexibility, the suppression of emotional expression, and symptoms of eating disorders and depression in adolescents with restrictive eating disorders referred for radically open dialectical behaviour therapy (RO DBT). Journal of eating disorders 0 41225620
2024 [Pilot study on the effect of an effective anger management group based on Dialectical Behavior Therapy (DBT)]. Vertex (Buenos Aires, Argentina) 0 39432007
1997 Resistance to infection with mouse hepatitis virus (MHV) in the cell clones derived from persistently infected DBT cells with the JHM strain of MHV. The Journal of veterinary medical science 0 9271448