| 1996 |
The CHAF1B (CAF1P60/CAF1A) gene was mapped to human chromosome 21q22.2, within the Down syndrome critical region, and encodes the p60 subunit of the CAF-1 complex that interacts with other subunits to promote histone assembly onto replicating DNA. |
Fluorescence in situ hybridization, somatic cell hybrids, YAC/cosmid hybridization, exon trapping |
Human genetics / Genomics |
Medium |
8660983 8792829
|
| 2015 |
CHAF1B is the medium (p60) subunit of the trimeric CAF-1 complex and contains a 7× WD repeat domain, a B-like domain, and a PEST domain; it functions as a histone H3/H4 chaperone shuttling newly synthesized histones to the replication fork during DNA synthesis. |
Review/synthesis of prior experimental literature on CAF-1 complex biochemistry |
Biochimica et biophysica acta |
Medium |
26066981
|
| 2018 |
Knockdown of CHAF1B in hepatocellular carcinoma (HUH-7) cells reduced invasion and migration ability, induced S-phase accumulation, and inhibited tumor growth in vivo; downstream gene expression changes included upregulation of PSMB6, SLC30A7, and SMC3, and downregulation of BLM and TWF2. |
Lentiviral shRNA knockdown, scratch wound healing assay, Transwell invasion assay, flow cytometry, gene expression profiling, Western blot, RT-PCR, xenograft mouse model |
Oncology reports |
Medium |
29767268
|
| 2019 |
CHAF1B promotes DNA damage repair in nasopharyngeal carcinoma cells following radiation, inhibiting apoptosis and conferring radioresistance; this mechanism is dependent on the DNA-PK pathway, as demonstrated by γH2AX foci resolution and DNA-PK inhibitor sensitization. |
siRNA/shRNA knockdown and overexpression, colony formation assay, γH2AX foci detection, flow cytometry (apoptosis), MTT assay, xenograft model, DNA-PK inhibitor treatment |
Biomedicine & pharmacotherapy |
Medium |
31869663
|
| 2020 |
CHAF1B acts as an E3 ubiquitin ligase that promotes ubiquitination and degradation of the nuclear co-repressor NCOR2; CHAF1B and NCOR2 physically interact predominantly in the nucleus, and CHAF1B-mediated NCOR2 degradation drives cisplatin resistance in lung adenocarcinoma. |
Proteome microarray, Western blot, co-immunoprecipitation, qRT-PCR, cell proliferation/migration assays, apoptosis assay, xenograft mouse model, immunohistochemistry, ubiquitination assay |
Cancer cell international |
Medium |
32508530
|
| 2021 |
CHAF1B depletion in mouse preimplantation embryos increases apoptosis, reduces blastocyst hatching and outgrowth, causes embryonic lethality post-implantation, and decreases expression of pluripotency factors (Oct4, Cdx2, Sox2, Nanog). Mechanistically, CHAF1B mediates replacement of histone H3.3 with H3.1/H3.2, associated with increased repressive marks (H3K9me2/3, H3K27me2/3) and decreased active marks (H3K4me2/3). |
siRNA knockdown in embryos, immunofluorescence, flow cytometry (apoptosis), RNA-sequencing, ATAC-sequencing, Western blot |
International journal of biological macromolecules |
Medium |
34906611
|
| 2023 |
CHAF1B binds directly to the TRIM13 promoter to repress its transcription in AML cells; loss of CHAF1B de-represses TRIM13, which then ubiquitinates CCNA1 to promote cell cycle entry and ultimately leukemic cell exhaustion, identifying the CHAF1B→TRIM13→CCNA1 axis as a key leukemogenic pathway. |
RNA sequencing, ChIP (CHAF1B promoter binding), knockdown/overexpression in AML cell lines and patient-derived xenografts, cell cycle and self-renewal assays |
Blood advances |
Medium |
37205848
|
| 2023 |
CHAF1B interacts with ULK1 (Unc-51-like kinase 1) in the nuclear compartment of MPN cells; silencing CHAF1B enhances transcription of IFNα-stimulated genes and potentiates IFNα-dependent antineoplastic responses in primary MPN progenitor cells. |
Co-immunoprecipitation/interaction studies, shRNA silencing, gene expression analysis, primary MPN progenitor cell functional assays |
Cancer research communications |
Medium |
37377894
|
| 2025 |
CHAF1B competitively binds to the SETD7 promoter region and represses its transcription in lung squamous-cell carcinoma, promoting cell proliferation; silencing CHAF1B upregulates SETD7 and suppresses tumor growth in vitro and in vivo. |
WGCNA bioinformatics, ChIP (promoter binding), CHAF1B knockdown, RNA sequencing, in vitro proliferation assays, xenograft model |
Frontiers of medicine |
Medium |
39862337
|
| 2025 |
CHAF1B promotes malignant phenotypes in hepatocellular carcinoma via activation of the PI3K/Akt/HIF-1α pathway; blockade of this pathway partially attenuates CHAF1B-mediated sorafenib resistance, placing CHAF1B upstream of PI3K/Akt/HIF-1α. |
RNA sequencing, pathway-specific inhibitors, siRNA knockdown, CCK8, colony formation, Transwell migration/invasion, flow cytometry, Western blot |
International journal of medical sciences |
Low |
41049428
|
| 2025 |
CHAF1B physically associates with BCL6 and TBL1XR1 in germinal center B cells (identified by Co-IP/MS); CHAF1B stabilizes the BCL6/TBL1XR1 repressor complex to cooperatively repress transcription, promote GC dark zone/light zone organization, and support GC B cell differentiation and antibody production. Loss of CHAF1B impairs GC formation, induces apoptosis, and reduces high-affinity antibody responses. |
Co-immunoprecipitation coupled with mass spectrometry, conditional knockout mouse models, flow cytometry, immunofluorescence, transcriptional reporter assays |
Journal of immunology |
High |
41764732
|
| 2025 |
Chaf1b upregulates IL-33 secretion in glioma stem cells, promoting microglial M2 polarization and activating the PI3K/AKT signaling pathway; neutralization of IL-33 reverses these effects, placing Chaf1b upstream of IL-33–PI3K/AKT in the GBM stem-immune axis. |
Genetic silencing in patient-derived GSCs, intracranial xenograft models, IL-33 neutralization, flow cytometry, self-renewal and tumorigenicity assays |
The Journal of neuroscience |
Medium |
41102004
|