| 2014 |
CENP-M is structurally and evolutionarily related to small GTPases but is incapable of GTP-binding and conformational switching (pseudo GTPase). CENP-M is crucially required for the assembly and stability of the HIKM tetramer comprising CENP-I, CENP-H, and CENP-K. A point mutant disrupting the CENP-M/CENP-I interaction hampers kinetochore assembly and chromosome alignment, and prevents kinetochore recruitment of the CENP-T/W complex, placing CENP-M downstream of CENP-C in a single kinetochore assembly pathway. |
Crystal structure, in vitro biochemical reconstitution, Co-IP/pulldown, active-site/interface mutagenesis, live-cell imaging, cell biological loss-of-function assays |
eLife |
High |
25006165
|
| 2006 |
An alternative transcript of the PANE1/CENPM gene encodes a novel HLA-A*0301-restricted minor histocompatibility antigen (mHAg) selectively expressed in resting CD19+ B cells and B-CLL cells. The antigenic peptide is encoded within a unique exon, and a single nucleotide polymorphism replacing an arginine codon with a stop codon determines differential T-cell recognition. Activation of B-CLL cells via CD40L decreases expression of the mHAg-encoding transcript and reciprocally increases expression of transcripts lacking this exon. |
Sequencing of PANE1 alleles, mass spectrometry peptide identification, T-cell recognition assays, RT-PCR expression analysis |
Blood |
Medium |
16391015
|
| 2019 |
CENPM knockdown in hepatocellular carcinoma cells inhibits cell proliferation in vitro and in vivo, suppresses migration and invasion, promotes apoptosis, and arrests the cell cycle. GSEA and functional validation linked CENPM activity to the p53 signaling pathway and cell cycle pathway. miR-1270 was identified as a negative post-transcriptional regulator of CENPM. |
siRNA knockdown, xenograft in vivo model, Western blot, flow cytometry, GSEA bioinformatics, miRNA luciferase reporter assay |
Journal of experimental & clinical cancer research |
Medium |
31703591
|
| 2020 |
CENPM knockdown in pancreatic cancer cells inhibits cell proliferation, alters the cell cycle, and limits cell migration and invasion via the mTOR/p70S6K signaling pathway. |
siRNA knockdown, cell proliferation assays, cell cycle analysis, migration/invasion assays, Western blot for mTOR/p70S6K pathway components |
Oncology reports |
Low |
32705259
|
| 2022 |
CENPM promotes lung adenocarcinoma cell cycle progression, proliferation, migration and invasion by activating phosphorylation of mTOR (not by altering total mTOR protein levels). Inhibition of mTOR activity abrogates the promoting effects of CENPM overexpression. |
Gain/loss-of-function assays, Western blot for mTOR phosphorylation, mTOR inhibitor rescue experiment, xenograft model |
Acta biochimica et biophysica Sinica |
Low |
35130633
|
| 2022 |
HPV E5 oncoprotein enhances CENPM expression through the transcription factor E2F1 binding to the CENPM promoter in head and neck squamous cell carcinoma. Inhibition of CENPM expression in HPV-positive HNSCC cells increases resistance to X-ray radiation (~59% under 2 Gy), associated with a reduced proportion of mitotic cells. |
ChIP assay for E2F1 binding at CENPM promoter, siRNA knockdown, CRISPR/Cas9 models, Western blot, qRT-PCR, clonogenic survival assay |
Oral oncology |
Medium |
35462155
|
| 2024 |
E2F1 acts as a key transcriptional regulator of CENPM in glioblastoma. CENPM promotes GBM cell proliferation, invasion, and glycolytic reprogramming (glucose consumption, lactate production, ATP levels). The glycolytic inhibitor 2-DG reverses CENPM overexpression effects, indicating dependence on glycolytic pathways. In vivo, CENPM knockdown reduced tumor volume by ~60%. |
shRNA knockdown, vector-based overexpression, glycolytic metabolite measurement, 2-DG pharmacological rescue, xenograft mouse model |
Cell biology and toxicology |
Low |
39707034
|
| 2024 |
CENPM knockdown in ovarian cancer cells activates the cGAS-STING pathway and promotes pyroptosis, suppressing proliferation, migration and invasion. A cGAS-STING pathway inhibitor reversed the inhibitory effects of CENPM knockdown on viability, migration and invasion, placing CENPM upstream of cGAS-STING in ovarian cancer cells. |
siRNA knockdown, pharmacological pathway inhibitor rescue, ELISA for inflammatory factors, Western blot, xenograft model |
BMC cancer |
Low |
38693472
|
| 2025 |
CENPM physically interacts with immune checkpoint ligand FGL1 in adrenocortical carcinoma cells. CENPM knockdown downregulates FGL1, suppresses ECM-related collagen signaling, and inhibits ACC cell invasion and migration; overexpression of FGL1 rescues the migration and invasion defects caused by CENPM knockdown. |
Co-immunoprecipitation, DIA quantitative proteomics, immunofluorescence colocalization, Western blot, rescue overexpression experiment, xenograft NPG mice |
Clinical and translational medicine |
Medium |
39778025
|
| 2025 |
CENPM knockdown in glioma cells induces G0/G1 phase cell cycle arrest and inhibits proliferation. RNA-seq and Western blot analyses demonstrate that CENPM activates the PI3K/AKT signaling pathway in glioma cells; in vivo knockdown reduces tumor growth. |
shRNA knockdown, RNA-seq, Western blot for PI3K/AKT pathway, cell cycle flow cytometry, xenograft model |
Genomics |
Low |
41317748
|