Affinage

CDC14A

Dual specificity protein phosphatase CDC14A · UniProt Q9UNH5

Length
594 aa
Mass
66.6 kDa
Annotated
2026-06-09
100 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/9 claims corpus-supported (89%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CDC14A is a dual-specificity protein phosphatase that selectively reverses CDK-mediated phosphorylation, with a strong intrinsic preference for phosphoserine over phosphothreonine at proline-directed (pSer-Pro) sites favoring a basic residue at +3 (PMID:12134069, PMID:22117071). This selectivity is structurally encoded: the family adopts two tandem DSP-like domains in which the C-terminal domain carries the catalytic PTP motif while the N-terminal domain shapes substrate specificity, creating a hydrophobic pocket that reads the pSer-Pro motif, and active-site residues including Asp253 act as both general acid and general base during catalysis (PMID:12853468, PMID:15128740). In human cells CDC14A localizes to interphase centrosomes through active nuclear export via an NES, and its dosage and activity control centrosome separation, spindle integrity, and cytokinesis (PMID:11901424, PMID:12134069). Beyond mitotic regulation, CDC14A acts on the actin cytoskeleton at the cell leading edge, where its phosphatase activity supports stress fibers, adhesion, and restrains migration through substrates including KIBRA and eplin, the latter dephosphorylated at ERK sites to maintain E-cadherin/catenin-based cell-cell adhesion (PMID:26747605, PMID:28465438). CDC14A also regulates primary cilium length via dephosphorylation of the actin-binding protein drebrin at CDK5-phosphorylated Ser142 and control of Arp2 recruitment to centrosomes (PMID:30467237). It modulates the G2/M transition by opposing CDK1-cyclin B1, dephosphorylating and inhibiting Cdc25B and stabilizing Wee1 through dephosphorylation of Ser123/Ser139 (PMID:20956543, PMID:23051732). Through dephosphorylation of hCdh1 at the inhibitory CDK site, CDC14A activates APC(Cdh1) but not APC(Cdc20) (PMID:11598127). CDC14A is also required for efficient repair of DNA double-strand breaks, acting redundantly with CDC14B in both homologous recombination and non-homologous end joining without affecting checkpoint signaling (PMID:20479464, PMID:26283732). In vivo, CDC14A phosphatase activity is essential for hearing and male fertility: it localizes to inner ear hair cell kinocilia and basal bodies, and biallelic loss-of-function or phosphatase-dead mutations cause sensorineural deafness in humans and deafness with male infertility in mice (PMID:29293958, PMID:27259055).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2000 Medium

    Established CDC14A as a phosphatase physically coupled to a key cell-cycle/tumor-suppressor substrate, showing it reverses CDK-type phosphorylation on a defined site.

    Evidence in vitro binding, co-IP, and in vitro phosphatase assays mapping p53 Ser315 dephosphorylation

    PMID:10644693

    Open questions at the time
    • Functional consequence of p53 Ser315 dephosphorylation in cells not established
    • single-lab in vitro dephosphorylation
  2. 2001 High

    Connected CDC14A to APC regulation by showing it dephosphorylates hCdh1 to selectively activate APC(Cdh1), defining a route by which it antagonizes mitotic CDK output.

    Evidence in vitro phosphatase assay and APC activity reconstitution with immunofluorescence

    PMID:11598127

    Open questions at the time
    • In vivo timing of APC(Cdh1) activation by CDC14A not resolved
    • selectivity over APC(Cdc20) mechanism not detailed
  3. 2002 High

    Defined CDC14A subcellular behavior and centrosome function, showing NES-dependent cytoplasmic localization to interphase centrosomes is required for centrosome separation and faithful division.

    Evidence siRNA knockdown, conditional overexpression, NES and phosphatase-dead mutants, live imaging, and in vitro CDK-substrate assays in human cells

    PMID:11901424 PMID:12134069

    Open questions at the time
    • Direct centrosomal substrate driving separation not identified
    • how NES export is regulated through the cycle unclear
  4. 2004 High

    Provided the structural and enzymological basis for CDC14 substrate selectivity and catalysis, explaining its preference for proline-directed phospho-sites.

    Evidence X-ray crystallography of yeast Cdc14, kinetic/pH-dependence and active-site mutagenesis including the human CDC14A substrate hCdh1; antibody injection in Xenopus embryos confirming a division requirement

    PMID:12853468 PMID:15128740 PMID:15251038

    Open questions at the time
    • Structure is of the yeast ortholog, not human CDC14A
    • in vivo catalytic mechanism for individual cellular substrates not dissected
  5. 2005 Medium

    Demonstrated functional conservation and a Cdc25-directed activity, linking CDC14A to mitotic-entry control via heterologous complementation.

    Evidence fission yeast complementation, co-IP, and in vitro/in vivo dephosphorylation of S. pombe Cdc25

    PMID:15911625

    Open questions at the time
    • Heterologous system; human Cdc25 relevance addressed only later
    • single lab
  6. 2007 Medium

    Expanded the substrate repertoire beyond cell-cycle regulators to ERK3 and the Rab5-GAP RN-tre, showing CDC14A reverses cell-cycle-dependent CDK phosphorylation on diverse targets.

    Evidence yeast two-hybrid, GST pull-down, substrate-trapping co-IP, and in vitro/in vivo phosphatase assays

    PMID:17371873 PMID:18235225

    Open questions at the time
    • Cellular phenotypes downstream of RN-tre/ERK3 dephosphorylation incompletely defined
    • substrate-trapping evidence from single lab
  7. 2010 High

    Distinguished CDC14A's roles in DNA repair versus checkpoint signaling and quantified its effect on the G2/M transition, while extending substrates to the actin cytoskeleton.

    Evidence gene knockouts in three vertebrate cell lines with γ-H2AX/comet assays; overexpression/knockdown cell-cycle analysis with in vitro Cdc25B dephosphorylation; native-locus phosphatase-dead knock-in with migration/adhesion assays and KIBRA rescue; in vitro RNA Pol II CTD assay

    PMID:20236090 PMID:20479464 PMID:20956543 PMID:22020438 PMID:26747605

    Open questions at the time
    • Direct DSB-repair substrate of CDC14A not identified
    • CTD phosphatase activity rests on a single in vitro assay
    • mechanism linking actin substrates to migration phenotype incomplete
  8. 2012 Medium

    Refined the G2/M control circuit by showing CDC14A stabilizes Wee1 and dephosphorylates KIBRA at defined CDK sites, tying it to mitotic-entry and mitotic-exit regulation.

    Evidence co-IP, in vitro phosphatase assays with site mutagenesis, siRNA depletion, and stability/cell-cycle assays

    PMID:22784093 PMID:23051732

    Open questions at the time
    • Quantitative contribution of Wee1 stabilization to mitotic timing not established
    • single-lab site mapping
  9. 2015 Medium

    Established functional redundancy with CDC14B in DSB repair across HR and NHEJ, clarifying why single-gene phenotypes can be subtle.

    Evidence knockout MEFs, siRNA, and IR-induced HR/NHEJ reporter assays

    PMID:26283732

    Open questions at the time
    • Direct CDC14A repair substrate not defined
    • Cdh1 link to repair only partially characterized
  10. 2017 High

    Identified eplin as a physiological CDC14A substrate at ERK sites, mechanistically linking CDC14A to maintenance of cadherin-based cell-cell adhesion.

    Evidence phosphoproteomics, BioID, in vitro/in vivo dephosphorylation, and phosphatase-dead knock-in cell lines

    PMID:28465438

    Open questions at the time
    • In vivo tissue context of eplin regulation not addressed
    • single lab
  11. 2018 High

    Defined CDC14A's ciliary function and its physiological essentiality, establishing it as a phosphatase required for cilium-length control, hearing, and male fertility through phosphatase-dependent mechanisms.

    Evidence phosphatase-dead knock-in RPE1 cells with phosphoproteomics (drebrin Ser142, Arp2); CRISPR phosphatase-dead/truncating mouse and zebrafish models with histology and ciliary localization; human genetics and zebrafish morpholino for deafness

    PMID:27259055 PMID:29293958 PMID:30467237

    Open questions at the time
    • Full set of ciliary substrates not enumerated
    • kinocilium-length phenotype differs between morphant and germ-line mutant models
    • molecular cause of postnatal hair-cell degeneration unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CDC14A's many substrate dephosphorylation events are spatially and temporally coordinated to produce its distinct roles in division, migration, ciliogenesis, DNA repair, and sensory/reproductive tissue maintenance remains unresolved.
  • No unified model linking centrosomal, cytoskeletal, and ciliary functions
  • regulation of CDC14A activity/localization across the cell cycle and across tissues not defined
  • direct DSB-repair substrate unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 10 GO:0016787 hydrolase activity 4
Localization
GO:0005815 microtubule organizing center 4 GO:0005730 nucleolus 3 GO:0005929 cilium 3 GO:0005634 nucleus 2 GO:0005856 cytoskeleton 2
Pathway
R-HSA-1640170 Cell Cycle 5 R-HSA-1266738 Developmental Biology 3 R-HSA-73894 DNA Repair 2
Partners
CDC25BERK3HCDH1KIBRAP53RN-TREWEE1

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 Human CDC14A phosphatase localizes to interphase centrosomes (not mitotic centrosomes), and this localization is independent of microtubules and phosphatase activity but requires active nuclear export via a nuclear export signal (NES). Disrupting the NES relocalizes CDC14A to nucleoli. Conditional overproduction of CDC14A (but not phosphatase-dead or NES-deficient mutants, or CDC14B) causes premature centrosome splitting and supernumerary mitotic spindles. siRNA-mediated knockdown of CDC14A impairs centrosome separation and cytokinesis, causing aberrant chromosome segregation. siRNA knockdown, conditional overexpression, NES mutagenesis, immunofluorescence localization, live-cell imaging Nature cell biology High 11901424
2002 Human CDC14A is selective for CDK substrates in vitro. CDC14A dynamically localizes to interphase (but not mitotic) centrosomes, whereas CDC14B localizes to the interphase nucleolus. Overexpression of CDC14A causes loss of pericentrin and γ-tubulin from centrosomes, mitotic spindle defects, chromosome segregation errors, and failure to complete cytokinesis. In vitro phosphatase assay with CDK substrates, immunofluorescence, overexpression in human cells Molecular biology of the cell High 12134069
2001 Human CDC14A (hCdc14a) dephosphorylates hCdh1, reversing cyclin B-Cdc2-mediated phosphorylation that prevents hCdh1 from activating the APC. hCdc14a activates APC(Cdh1) but does not affect APC(Cdc20) activity. hCdc14a localizes to centrosomes in HeLa cells. In vitro phosphatase assay, APC activity reconstitution, immunofluorescence The Journal of biological chemistry High 11598127
2000 Human CDC14A and CDC14B interact with tumor suppressor p53 both in vitro and in vivo; this interaction requires the N-termini of the hCdc14 proteins and the C-terminus of p53. Both phosphatases specifically dephosphorylate p53 at the p34(Cdc2)/Clb phosphorylation site Ser315. In vitro binding assay, co-immunoprecipitation, in vitro phosphatase assay with p53 The Journal of biological chemistry Medium 10644693
2007 Human CDC14A binds directly to the atypical MAPK ERK3 via ERK3's unique C-terminal domain. CDC14A can remove CDK-mediated phosphorylation from ERK3 in vitro. CDC14A forms a stable complex with ERK3 in human cells independently of its phosphatase activity, mediated by CDC14A's C-terminal regulatory domain. CDC14A upregulation redistributes the ERK3 substrate MK5 from nucleus to cytoplasm and stabilizes ERK3-cyclin D3 complex formation. Yeast two-hybrid screen, GST pull-down, co-immunoprecipitation, in vitro phosphatase assay, subcellular localization imaging Cell cycle Medium 18235225
2007 RN-tre, a Rab5 GTPase-activating protein, is identified as a substrate of human CDC14A. A substrate-trapping mutant (CDC14A C278S) stably associates with RN-tre in human cells. RN-tre undergoes cell cycle-dependent phosphorylation peaking at mitosis, which CDC14A reverses in vitro and in vivo. RN-tre is a direct CDK substrate and CDC14A dephosphorylates proline-directed CDK phosphorylation sites. RN-tre phosphorylation modulates its catalytic activity. Substrate-trapping Co-IP, in vitro phosphatase assay, tungstate competition, cell cycle phosphorylation analysis The Journal of biological chemistry Medium 17371873
2010 Human CDC14A associates with the actin cytoskeleton of human cells. Ablation of CDC14A phosphatase activity (phosphatase-dead knock-in) diminishes stress fibers, increases cell migration speed, and reduces cell adhesion. CDC14A colocalizes with its substrate KIBRA at the cell leading edge, and KIBRA overexpression rescues CDC14A phosphatase-dead phenotypes. Phosphatase-dead knock-in at native locus, immunofluorescence, cell migration assays, adhesion assays, co-localization Proceedings of the National Academy of Sciences of the United States of America High 26747605
2017 Human CDC14A dephosphorylates eplin (epithelial protein lost in neoplasm) at ERK-phosphorylated sites Ser362 and Ser604, counteracting EGF-induced actin cytoskeleton rearrangements. CDC14A phosphatase-dead (PD) cells and eplin knockout cells both show reduced E-cadherin and α/β-catenin at cell-cell adhesions. Phospho-proteome profiling, proximity-dependent biotin identification (BioID), in vitro and in vivo dephosphorylation assays, phosphatase-dead knock-in cell lines Proceedings of the National Academy of Sciences of the United States of America High 28465438
2018 Human CDC14A regulates primary cilia length by dephosphorylating the actin-binding protein drebrin at CDK5-phosphorylated Ser142, and by regulating recruitment of the actin organizer Arp2 to centrosomes. CDC14A phosphatase-dead RPE1 cells have longer cilia than wild-type, while CDC14A overexpression reduces cilia formation. CDC14A also regulates endocytosis and targeting of myosin Va vesicles to the basal body during ciliogenesis. Phosphatase-dead knock-in, phospho-proteome analysis, immunofluorescence, cilia length measurement, overexpression EMBO reports High 30467237
2018 CDC14A phosphatase activity is essential in vivo for hearing and male fertility in mammals. CRISPR/Cas9 phosphatase-dead (p.C278S) mouse Cdc14a mutations cause deafness and male infertility. CDC14A protein localizes to inner ear hair cell kinocilia, basal bodies, and stereocilia. Auditory hair cells develop normally but degenerate postnatally in mutants. In males, degeneration of seminiferous tubules and spermiation defects lead to low sperm count and abnormal sperm motility/morphology. CRISPR/Cas9 phosphatase-dead and truncating knock-in mouse models, zebrafish mutants, immunofluorescence localization, histology Human molecular genetics High 29293958
2016 Biallelic loss-of-function mutations in CDC14A (nonsense alleles) cause severe-to-profound sensorineural deafness in humans. CDC14A protein is strongly expressed in kinocilia of inner ear hair cells in mouse. Morpholino knockdown of cdc14a in zebrafish reduces kinocilium length in inner ear hair cells, indicating that CDC14A is required for proper kinocilium growth/maintenance in hair bundles. Human genetic linkage analysis and whole-exome sequencing, mouse immunofluorescence, zebrafish morpholino knockdown and kinocilium length measurement American journal of human genetics Medium 27259055
2010 Vertebrate cells with CDC14A gene deletion (chicken DT40, human HCT116, human RPE1) are DNA damage checkpoint proficient and arrest normally in G2 after irradiation with normal Chk1/Chk2 activation. However, CDC14A-KO cells show persistent γ-H2AX foci and slower resolution of DNA double-strand breaks, indicating CDC14A is required for efficient DNA repair but not checkpoint signaling. Gene knockout (targeted deletion) in three cell lines, irradiation assay, γ-H2AX foci quantification, comet assay, immunoblot for checkpoint kinase activation The Journal of cell biology High 20479464
2015 CDC14A and CDC14B redundantly regulate DNA double-strand break repair in mammalian cells. CDC14B-KO MEFs show DSB repair defects at late passages when CDC14A levels are also low. Combined loss of both CDC14A and CDC14B impairs both homologous recombination and non-homologous end joining. Cdh1 is a downstream target of Cdc14B in DSB repair. Knockout MEFs, siRNA knockdown, IR-induced DSB repair assays (γ-H2AX, HR and NHEJ reporters), immunoblot Molecular and cellular biology Medium 26283732
2010 Human CDC14A modulates the G2/M transition: increasing CDC14A levels delays mitotic entry by inhibiting Cdk1-cyclin B1 activity, whereas depleting CDC14A accelerates mitotic entry. CDC14A directly binds to and dephosphorylates Cdc25B, inhibiting its catalytic activity. CDC14A also regulates Cdc25A activity at the G2/M transition. Overexpression and siRNA knockdown in human cells, in vitro phosphatase assay with Cdc25B, cell cycle analysis, Cdk1 kinase activity assay The Journal of biological chemistry Medium 20956543
2012 Human CDC14A binds to Wee1 at its amino-terminal domain and dephosphorylates Wee1 at CDK-phosphorylated Ser123 and Ser139, preventing Plk1-mediated phosphorylation and subsequent Wee1 degradation at mitotic entry. CDC14A depletion significantly reduces Wee1 protein levels. Co-immunoprecipitation, in vitro phosphatase assay with recombinant Wee1, siRNA depletion, phospho-site mutagenesis, Wee1 stability assay Molecular biology of the cell Medium 23051732
2012 CDC14A and CDC14B phosphatases associate with KIBRA in human cells and dephosphorylate CDK1-phosphorylated KIBRA in vitro and in cells. CDK1 phosphorylates KIBRA at Ser542 and Ser931. Phospho-regulation of KIBRA by CDK1 and CDC14 is involved in mitotic exit under spindle stress. Co-immunoprecipitation, in vitro kinase/phosphatase assays, site-directed mutagenesis, inducible expression, cell cycle analysis The Biochemical journal Medium 22784093
2009 Mouse CDC14A concentrates in the nucleus of meiotically incompetent oocytes but disperses throughout meiotically competent oocytes. Between metaphase I and metaphase II, CDC14A co-localizes with the central portion of the meiotic spindle. Antibody microinjection against CDC14A specifically delays exit from meiosis I and generates eggs with chromosome alignment abnormalities and elevated aneuploidy. Overexpression of CDC14A delays meiotic progression after meiosis resumption. Immunofluorescence localization in mouse oocytes, antibody microinjection, overexpression, chromosome spread analysis Cell cycle Medium 19270517
2010 Human CDC14A (like budding yeast Cdc14) can act as a CTD phosphatase for RNA polymerase II, targeting CTD phosphorylation at Ser2 and Ser5, consistent with a conserved role in transcriptional repression. In vitro CTD phosphatase assay with human CDC14A Nature cell biology Low 22020438
2004 Xenopus CDC14A (XCdc14alpha) localizes to the nucleolus in interphase and to mitotic centrosomes. XCdc14beta localizes primarily to centrosomes. Antibody injection against XCdc14alpha/beta into Xenopus embryos at the two-cell stage blocks division of injected blastomeres, demonstrating a requirement for CDC14A/B activity in vertebrate cell division. XCdc14alpha is phosphorylated both meiotically and mitotically. GFP tagging, immunofluorescence, antibody microinjection into Xenopus embryos BMC cell biology Medium 15251038
2010 ERK3 C-terminal phosphorylation sites (Ser684, Ser688, Thr698, Ser705), all proline-directed, are phosphorylated by cyclin B-Cdk1. CDC14A and CDC14B both bind ERK3 and reverse this C-terminal phosphorylation in mitosis. Alanine substitution of these four sites decreases ERK3 half-life in mitosis, linking CDC14A/B-opposed phosphorylation to ERK3 stabilization. Mass spectrometry site identification, in vitro kinase/phosphatase assays with purified proteins, co-immunoprecipitation, pulse-chase stability assay The Biochemical journal Medium 20236090
2005 Human CDC14A rescues flp1-deficient fission yeast strains, demonstrating functional homology. CDC14A interacts in vivo with S. pombe Cdc25 and dephosphorylates Cdc25 both in vitro and in vivo, suggesting CDC14A can inhibit Cdc25 activity. Complementation assay in fission yeast, co-immunoprecipitation, in vitro phosphatase assay The Journal of biological chemistry Medium 15911625
2004 Crystal structure of Cdc14 (S. cerevisiae, relevant to the conserved family including CDC14A) reveals two tandem DSP-like domains. The C-terminal domain contains the catalytic PTP motif; the N-terminal domain contributes to substrate specificity but lacks catalytic activity. The active site is at the interface of both domains. The structure reveals a hydrophobic pocket specifying the pSer-Pro motif, explaining selectivity for proline-directed phosphorylation. X-ray crystallography, kinetic analysis, peptide substrate binding The EMBO journal High 12853468
2004 Detailed kinetic and mechanistic analysis of Cdc14 phosphatase (including hCdc14a with substrate hCdh1) reveals: general acid-independent mechanism for substrates with pKa < 7, and general acid-dependent mechanism for substrates with pKa > 7. Asp253 functions as the general acid during phosphoenzyme formation and as the general base during hydrolysis. Asp50, Asp129, Glu168, Glu171, and Asp177 are required for efficient dephosphorylation of hCdh1. Kinetic analysis, mutagenesis of active-site residues, pre-steady-state kinetics, pH-dependence studies The Journal of biological chemistry High 15128740
2011 Cdc14 family phosphatases, including CDC14A homologs, have a strong and conserved preference for phosphoserine over phosphothreonine at proline-directed (CDK) sites. An invariant active-site residue sterically restricts phosphothreonine binding, responsible for phosphoserine selectivity. Optimal substrates additionally possess a basic residue at the +3 position. This selectivity is demonstrated both in vitro with purified proteins and in vivo using serine-to-threonine substitutions. In vitro phosphatase assays with phosphopeptides and phosphoprotein substrates, site-directed mutagenesis of active-site residue and substrate sites, in vivo analysis The Journal of biological chemistry High 22117071

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1999 Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 631 10219244
1999 Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 514 10235265
2002 Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase. Cell 385 11832211
2004 Closing mitosis: the functions of the Cdc14 phosphatase and its regulation. Annual review of genetics 367 15568976
1999 Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14. Current biology : CB 343 10074450
2004 Cdc14 and condensin control the dissolution of cohesin-independent chromosome linkages at repeated DNA. Cell 248 15137939
2003 Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14. Science (New York, N.Y.) 224 14605209
2004 Cdc14 phosphatase induces rDNA condensation and resolves cohesin-independent cohesion during budding yeast anaphase. Cell 221 15137940
2002 Deregulated human Cdc14A phosphatase disrupts centrosome separation and chromosome segregation. Nature cell biology 169 11901424
2000 Cdc14 activates cdc15 to promote mitotic exit in budding yeast. Current biology : CB 155 10837230
2004 Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. Science (New York, N.Y.) 153 15273393
2010 Cdc14: a highly conserved family of phosphatases with non-conserved functions? Journal of cell science 141 20720150
1992 Addition of extra origins of replication to a minichromosome suppresses its mitotic loss in cdc6 and cdc14 mutants of Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 140 1557417
2003 The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase. The EMBO journal 133 12853468
2002 Disruption of centrosome structure, chromosome segregation, and cytokinesis by misexpression of human Cdc14A phosphatase. Molecular biology of the cell 132 12134069
2001 Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis. Journal of cell science 129 11683392
2007 Cdc14-regulated midzone assembly controls anaphase B. The Journal of cell biology 127 17562791
2004 At the interface between signaling and executing anaphase--Cdc14 and the FEAR network. Genes & development 115 15520278
2003 The Cdc14 phosphatase and the FEAR network control meiotic spindle disassembly and chromosome segregation. Developmental cell 110 12737806
2009 Cdc14 inhibits transcription by RNA polymerase I during anaphase. Nature 100 19158678
2009 Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. The Journal of cell biology 92 19221193
1992 CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle. The Journal of biological chemistry 92 1597462
2010 Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repair. The Journal of cell biology 89 20479464
2014 Dual control of Yen1 nuclease activity and cellular localization by Cdk and Cdc14 prevents genome instability. Molecular cell 88 24631285
2001 Regulation of the anaphase-promoting complex by the dual specificity phosphatase human Cdc14a. The Journal of biological chemistry 87 11598127
2000 The human Cdc14 phosphatases interact with and dephosphorylate the tumor suppressor protein p53. The Journal of biological chemistry 86 10644693
2008 The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. The Journal of cell biology 83 18378776
2003 The role of the polo kinase Cdc5 in controlling Cdc14 localization. Molecular biology of the cell 82 14551257
2002 Mitotic exit network controls the localization of Cdc14 to the spindle pole body in Saccharomyces cerevisiae. Current biology : CB 82 12062061
2004 The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans. Nature cell biology 79 15247923
2014 The Cdk/cDc14 module controls activation of the Yen1 holliday junction resolvase to promote genome stability. Molecular cell 77 24631283
2010 Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator. Cell 75 20403323
2008 APC/C-Cdh1-mediated degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus. Genes & development 75 18172166
1993 The Schizosaccharomyces pombe cdc14 gene is required for septum formation and can also inhibit nuclear division. Molecular biology of the cell 74 8334307
2011 Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine. The Journal of biological chemistry 73 22117071
2003 Cell cycle regulator Cdc14 is expressed during sporulation but not hyphal growth in the fungus-like oomycete Phytophthora infestans. Molecular microbiology 73 14617173
2002 Control of Lte1 localization by cell polarity determinants and Cdc14. Current biology : CB 71 12498684
2008 The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1. Current biology : CB 67 18951025
2007 Evolution of Ime2 phosphorylation sites on Cdk1 substrates provides a mechanism to limit the effects of the phosphatase Cdc14 in meiosis. Molecular cell 66 17349956
2001 Characterization of the Net1 cell cycle-dependent regulator of the Cdc14 phosphatase from budding yeast. The Journal of biological chemistry 64 11274204
2010 Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes. The Journal of biological chemistry 63 21127052
2008 Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein. PLoS biology 62 18547142
2002 Budding yeast Cdc5 phosphorylates Net1 and assists Cdc14 release from the nucleolus. Biochemical and biophysical research communications 57 12056824
2004 Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6. Molecular and cellular biology 56 14993267
2011 Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis. Molecular biology of the cell 55 22072794
2008 Separase cooperates with Zds1 and Zds2 to activate Cdc14 phosphatase in early anaphase. The Journal of cell biology 54 18762578
2011 Cdc14 phosphatase promotes segregation of telomeres through repression of RNA polymerase II transcription. Nature cell biology 53 22020438
2004 A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast. Journal of cell science 52 15128870
2006 Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe. Developmental cell 48 16950131
2004 The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase. Developmental cell 47 15525536
2019 Role of protein phosphatases PP1, PP2A, PP4 and Cdc14 in the DNA damage response. Cell stress 46 31225502
2018 CDC14A phosphatase is essential for hearing and male fertility in mouse and human. Human molecular genetics 45 29293958
2013 Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Molecular & cellular proteomics : MCP 45 23297348
2010 Oscillations in Cdc14 release and sequestration reveal a circuit underlying mitotic exit. The Journal of cell biology 45 20660629
2005 Crm1-mediated nuclear export of Cdc14 is required for the completion of cytokinesis in budding yeast. Cell cycle (Georgetown, Tex.) 45 15917648
2010 The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase. The Journal of cell biology 43 21098112
1996 Schizosaccharomyces pombe skp1+ encodes a protein kinase related to mammalian glycogen synthase kinase 3 and complements a cdc14 cytokinesis mutant. Molecular and cellular biology 43 8524294
2012 Cdc14-dependent dephosphorylation of Inn1 contributes to Inn1-Cyk3 complex formation. Journal of cell science 42 22454527
2005 Functional homology among human and fission yeast Cdc14 phosphatases. The Journal of biological chemistry 42 15911625
2019 Cdc14 and PP2A Phosphatases Cooperate to Shape Phosphoproteome Dynamics during Mitotic Exit. Cell reports 41 31722221
1996 Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the lte1 defect in termination of M phase and genetically interact with CDC14. Molecular & general genetics : MGG 40 8668128
2007 A functional link between the human cell cycle-regulatory phosphatase Cdc14A and the atypical mitogen-activated kinase Erk3. Cell cycle (Georgetown, Tex.) 36 18235225
2002 Cell cycle: new functions for Cdc14 family phosphatases. Current biology : CB 35 12419203
2013 Cytokinesis-required Cdc14 is a signaling hub of asexual development and multi-stress tolerance in Beauveria bassiana. Scientific reports 34 24169500
2016 Mutations in CDC14A, Encoding a Protein Phosphatase Involved in Hair Cell Ciliogenesis, Cause Autosomal-Recessive Severe to Profound Deafness. American journal of human genetics 32 27259055
2015 Dephosphorylation of Iqg1 by Cdc14 regulates cytokinesis in budding yeast. Molecular biology of the cell 32 26085509
2014 Cdc14 targets the Holliday junction resolvase Yen1 to the nucleus in early anaphase. Cell cycle (Georgetown, Tex.) 32 24626187
2008 Cdc28 and Cdc14 control stability of the anaphase-promoting complex inhibitor Acm1. The Journal of biological chemistry 32 18287090
2016 Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion. Proceedings of the National Academy of Sciences of the United States of America 31 26747605
2012 Phospho-regulation of KIBRA by CDK1 and CDC14 phosphatase controls cell-cycle progression. The Biochemical journal 31 22784093
2018 The human phosphatase CDC14A modulates primary cilium length by regulating centrosomal actin nucleation. EMBO reports 30 30467237
2015 FEAR-mediated activation of Cdc14 is the limiting step for spindle elongation and anaphase progression. Nature cell biology 30 25706236
2010 Human Cdc14A phosphatase modulates the G2/M transition through Cdc25A and Cdc25B. The Journal of biological chemistry 30 20956543
2009 Essential global role of CDC14 in DNA synthesis revealed by chromosome underreplication unrecognized by checkpoints in cdc14 mutants. Proceedings of the National Academy of Sciences of the United States of America 30 19666479
2006 Novel role for Cdc14 sequestration: Cdc14 dephosphorylates factors that promote DNA replication. Molecular and cellular biology 30 17116692
2018 A PxL motif promotes timely cell cycle substrate dephosphorylation by the Cdc14 phosphatase. Nature structural & molecular biology 29 30455435
2021 Down-regulation of circular RNA CDC14A peripherally ameliorates brain injury in acute phase of ischemic stroke. Journal of neuroinflammation 28 34876161
2008 Cds1 controls the release of Cdc14-like phosphatase Flp1 from the nucleolus to drive full activation of the checkpoint response to replication stress in fission yeast. Molecular biology of the cell 28 18385517
2005 Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation. Current biology : CB 28 16085490
2018 The Aspergillus flavus Phosphatase CDC14 Regulates Development, Aflatoxin Biosynthesis and Pathogenicity. Frontiers in cellular and infection microbiology 27 29868497
2017 Human phosphatase CDC14A regulates actin organization through dephosphorylation of epithelial protein lost in neoplasm. Proceedings of the National Academy of Sciences of the United States of America 27 28465438
2008 The role of Cdc14 phosphatases in the control of cell division. Biochemical Society transactions 27 18481975
2004 Xenopus Cdc14 alpha/beta are localized to the nucleolus and centrosome and are required for embryonic cell division. BMC cell biology 27 15251038
2010 C-terminal domain phosphorylation of ERK3 controlled by Cdk1 and Cdc14 regulates its stability in mitosis. The Biochemical journal 26 20236090
2009 The CDC14A phosphatase regulates oocyte maturation in mouse. Cell cycle (Georgetown, Tex.) 26 19270517
2008 Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit. Molecular biology of the cell 26 18923139
2007 Regulation of multiple cell cycle events by Cdc14 homologues in vertebrates. Experimental cell research 26 17292885
2004 Kinetic and mechanistic studies of a cell cycle protein phosphatase Cdc14. The Journal of biological chemistry 26 15128740
2020 The Multiple Roles of the Cdc14 Phosphatase in Cell Cycle Control. International journal of molecular sciences 25 31973188
2015 Cdc14A and Cdc14B Redundantly Regulate DNA Double-Strand Break Repair. Molecular and cellular biology 25 26283732
2012 The Mitotic Exit Network and Cdc14 phosphatase initiate cytokinesis by counteracting CDK phosphorylations and blocking polarised growth. The EMBO journal 25 22872148
2012 Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation. Molecular biology of the cell 25 23051732
2017 Re-examining the role of Cdc14 phosphatase in reversal of Cdk phosphorylation during mitotic exit. Journal of cell science 24 28663385
2016 Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair. The EMBO journal 24 27852625
2007 Regulation of the Rab5 GTPase-activating protein RN-tre by the dual specificity phosphatase Cdc14A in human cells. The Journal of biological chemistry 24 17371873
2008 The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell cycle (Georgetown, Tex.) 23 18927509
2015 Cdc14 phosphatase: warning, no delay allowed for chromosome segregation! Current genetics 22 26116076
2008 A nucleolus-localized activator of Cdc14 phosphatase supports rDNA segregation in yeast mitosis. Current biology : CB 22 18595708
2015 Synthetic physical interactions map kinetochore regulators and regions sensitive to constitutive Cdc14 localization. Proceedings of the National Academy of Sciences of the United States of America 21 26240346
2009 The molecular function of the yeast polo-like kinase Cdc5 in Cdc14 release during early anaphase. Molecular biology of the cell 21 19570916

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