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Showing NCBP1 — CBP80 is a alias.

NCBP1

Nuclear cap-binding protein subunit 1 · UniProt Q09161

Length
790 aa
Mass
91.8 kDa
Annotated
2026-06-10
33 papers in source corpus 23 papers cited in narrative 23 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NCBP1 (CBP80) is the large subunit of the nuclear cap-binding complex that binds the 5'-cap of newly synthesized mRNA and coordinates downstream steps of mRNA biogenesis, from co-transcriptional loading through nuclear export, splicing, and a pioneer round of translation (PMID:11551508, PMID:12093754). It associates with intron-containing RNA and the RNA polymerase II C-terminal domain, and CBP80-bound mRNPs carry exon-junction complex components and NMD factors into the cytoplasm, whereas eIF4E-bound mRNPs do not (PMID:12093754). During the pioneer round, CBP80-bound mRNAs are the substrate for nonsense-mediated decay: NCBP1 directly binds UPF1 and promotes UPF1-UPF2 interaction, driving SURF complex assembly with SMG1 and release factors at premature termination codons and subsequent engagement of the exon-junction complex (PMID:16186820, PMID:20691628). NCBP1 directs cap-dependent translation of these mRNPs by recruiting the dedicated initiation factor CTIF, which bridges NCBP1 to eIF3g for ribosome recruitment (PMID:19648179, PMID:22493286). Beyond the canonical CBP80-CBP20 partnership, NCBP1 forms an alternative cap-binding complex with NCBP3 that supports poly(A) RNA nuclear export and is essential for cell viability (PMID:26382858). NCBP1 also serves as a sensor of nuclear electrophile stress through cysteine C436: modification of this residue disrupts the NCBP1-SF3A1 interaction, triggering widespread alternative splicing and translational repression (PMID:41667655). In several cancer and disease contexts, NCBP1 has been linked to mRNA stabilization programs, including an NCBP1-NCBP3-CUL4B axis in lung cancer, METTL3/m6A-dependent c-MYC regulation in lymphoma, and IGF2BP3-recruited stabilization of CDK6 mRNA (PMID:31448526, PMID:37244946, PMID:38945255).

Mechanistic history

Synthesis pass · year-by-year structured walk · 22 steps
  1. 1995 Medium

    Established how NCBP1 achieves cap binding, showing it does not act alone but requires partner subunits with RNA-binding capacity.

    Evidence Yeast two-hybrid screen and cap-binding assay identifying NIP1/2/3 interactors

    PMID:7478990

    Open questions at the time
    • Identity of NIPs relative to the later-defined CBP20/NCBP2 not resolved here
    • Functional consequences of the cap binding in vivo not tested
  2. 2001 High

    Defined NCBP1 as the mark of newly synthesized 'pioneer' mRNPs and showed these, not eIF4E-bound mRNAs, are the substrate for NMD, linking cap identity to mRNA surveillance.

    Evidence Reciprocal immunopurification of CBP80- vs eIF4E-bound mRNPs with cycloheximide and suppressor tRNA inhibition

    PMID:11551508

    Open questions at the time
    • Direct molecular bridge from CBP80 to NMD factors not yet defined
    • Translation initiation mechanism for CBP80-mRNPs unknown
  3. 2002 High

    Connected NCBP1 to co-transcriptional loading and cytoplasmic cargo delivery, showing CBP80-mRNPs associate with Pol II CTD and carry EJC/NMD components after export.

    Evidence Immunoprecipitation with nuclear/cytoplasmic fractionation and Western blotting

    PMID:12093754

    Open questions at the time
    • Order of EJC versus CBC loading not resolved
    • Direct versus indirect CTD association not distinguished
  4. 2005 High

    Provided the molecular basis for CBP80-dependent NMD specificity by showing NCBP1 directly binds UPF1 and promotes UPF1-UPF2, while not affecting Staufen-mediated decay.

    Evidence Reciprocal Co-IP, siRNA knockdown, and NMD versus SMD efficiency assays

    PMID:16186820

    Open questions at the time
    • Structural detail of NCBP1-UPF1 interface unresolved
    • How PTC recognition is communicated to CBP80 not shown
  5. 2009 High

    Identified CTIF as the dedicated initiation factor for CBP80-bound mRNAs, explaining how the pioneer round is translated distinctly from steady-state eIF4E-dependent translation.

    Evidence Co-IP, in vitro translation depletion/reconstitution with purified CTIF, siRNA, and NMD assay

    PMID:19648179

    Open questions at the time
    • Mechanism of ribosome recruitment downstream of CTIF not yet defined
  6. 2009 Medium

    Extended NCBP1 pioneer mRNPs to neuronal local translation, showing CBP80/LSm1 mRNPs relocate to dendritic spines upon synaptic stimulation.

    Evidence Immunofluorescence, subcellular fractionation, and glutamatergic stimulation

    PMID:19188494

    Open questions at the time
    • Direct demonstration of translational activation of these specific mRNPs not shown
    • Functional outcome on synaptic protein synthesis untested
  7. 2010 High

    Dissected two mechanistic steps by which UPF1-NCBP1 binding drives NMD, ordering SURF complex formation and EJC engagement at the PTC.

    Evidence Dominant-negative UPF1-CBP80 disruption mutant, Co-IP, mRNA binding, and NMD reporters

    PMID:20691628

    Open questions at the time
    • Kinetics of SURF-to-EJC transition not resolved
    • Role of CBP80 conformation untested
  8. 2008 Medium

    Showed the CBP80 pioneer round and its coupled NMD apply even to IRES-initiated translation, generalizing the surveillance role across initiation modes.

    Evidence EMCV IRES NMD reporters with CBP80 vs eIF4E mRNP fractionation

    PMID:18369367

    Open questions at the time
    • How IRES initiation engages CBP80 mechanistically unclear
  9. 2011 Medium

    Identified Ago2 as a competitive regulator of CBP80 cap binding, linking miRNA machinery to suppression of pioneer-round translation and coupled NMD.

    Evidence Tethering assays, CBP80 Co-IP, NMD reporters, and cap-association-deficient Ago2 mutant

    PMID:21840310

    Open questions at the time
    • Physiological extent of Ago2-CBC competition not quantified
  10. 2011 High

    Established a conserved cytoplasmic translation role for the yeast CBP80 ortholog under stress, showing it is required for rapid polysome reassociation of osmostress mRNAs.

    Evidence Polysome fractionation, cbc1delta genetic epistasis with eIF4E, cycloheximide sensitivity, and stress-induced relocalization

    PMID:22072789

    Open questions at the time
    • Direct conservation of this stress translation function in human NCBP1 not tested
  11. 2010 Medium

    Defined dual splicing functions of the CBP80 ortholog, promoting both U1 5' splice site recognition and U2 snRNP recruitment.

    Evidence Yeast cbp80 deletion suppressor screen and splicing assays

    PMID:20801768

    Open questions at the time
    • Mechanistic basis of U2 recruitment dependence on 5'SS-proximal sequences unresolved
    • Conservation in human splicing not directly tested here
  12. 2012 High

    Completed the ribosome-recruitment chain by showing CTIF bridges NCBP1 to eIF3g, mechanistically distinguishing CBP80/20-dependent translation from eIF4E-dependent translation.

    Evidence Co-IP, polysome fractionation, CTIF tethering driving downstream cistron translation, and NMD reporter

    PMID:22493286

    Open questions at the time
    • Structural model of the NCBP1-CTIF-eIF3 assembly not resolved
  13. 2015 High

    Resolved the essentiality and export functions of NCBP1, showing it alone (not CBP20) is required for viability and poly(A) export and can form an alternative complex with NCBP3.

    Evidence Knockout viability, FISH RNA export assays, mass spectrometry interactome, and Co-IP

    PMID:26382858

    Open questions at the time
    • Division of labor between NCBP2 and NCBP3 partnerships under different conditions not fully mapped
  14. 2018 Medium

    Showed NCBP1 is co-opted by HIV-1 Rev to support nuclear export and translation of unspliced viral mRNA via a CBP80-eIF4AI complex.

    Evidence Co-IP, RNA-IP, and export/translation assays with Rev/RRE

    PMID:30239828

    Open questions at the time
    • Whether NCBP1 directly binds Rev versus via the mRNP not fully resolved
  15. 2020 Medium

    Revealed a regulatory counterpoint in which CTIF competes with Rev for CBP80 binding and inhibits HIV-1 Gag synthesis.

    Evidence Co-IP, fractionation, siRNA, and translation reporters

    PMID:33103564

    Open questions at the time
    • In vivo balance of CTIF-Rev competition during infection unquantified
  16. 2019 Medium

    Linked NCBP1 to cap-binding-complex roles in transcription, showing the Drosophila ortholog with Paip2 supports Pol II CTD Ser5 phosphorylation at active promoters.

    Evidence Co-IP, ChIP, and Pol II CTD phosphorylation assays in Drosophila

    PMID:31001806

    Open questions at the time
    • Conservation of this promoter function in human NCBP1 not established
  17. 2017 Medium

    Connected NCBP1 to small-RNA pathways, showing the Drosophila ortholog is required for Piwi/Aub/Ago3 stability and piRNA biogenesis.

    Evidence Germline RNAi, small RNA sequencing, and immunofluorescence

    PMID:28746365

    Open questions at the time
    • Direct molecular substrate of CBC in piRNA biogenesis unresolved
    • Human relevance not tested
  18. 2019 Medium

    Implicated NCBP1 in oncogenic mRNA programs via an NCBP1-NCBP3-CUL4B axis promoting lung cancer growth.

    Evidence Co-IP, knockdown/overexpression, and CUL4B rescue in cell growth assays

    PMID:31448526

    Open questions at the time
    • Mechanism of CUL4B upregulation by the complex not detailed
  19. 2023 Medium

    Extended NCBP1's mRNA-stabilization role to m6A regulation, showing it stabilizes METTL3 mRNA to enhance c-MYC m6A modification in lymphoma.

    Evidence Co-IP, siRNA, m6A and mRNA stability assays, proliferation assays

    PMID:37244946

    Open questions at the time
    • Direct versus indirect basis of METTL3 mRNA stabilization unresolved
  20. 2024 Medium

    Showed NCBP1 acts as an m6A-reader-recruited stabilizer, where IGF2BP3 recruits it to the CDK6 5'UTR to suppress senescence.

    Evidence IP-MS, m6A site mapping, mRNA stability, and rescue assays

    PMID:38945255

    Open questions at the time
    • Generalizability of IGF2BP3-NCBP1 stabilization to other transcripts untested
  21. 2026 High

    Identified NCBP1 as a nuclear electrophile-stress sensor, where modification of cysteine C436 disrupts NCBP1-SF3A1 binding to reprogram alternative splicing and inhibit translation.

    Evidence Precision electrophile generation, genetic code expansion, C436 mutagenesis, Co-IP, splicing sequencing, and polysome assays

    PMID:41667655

    Open questions at the time
    • Endogenous electrophiles engaging C436 in vivo not enumerated
    • Link between specific spliced isoforms and translation block beyond S6 kinase not fully mapped
  22. 2026 Medium

    Demonstrated a developmental requirement, showing Ncbp1 depletion causes morula arrest with nuclear poly(A) retention and lipid-metabolic deficits rescuable by oleic acid.

    Evidence Zygotic siRNA, poly(A) FISH, RNA-seq, proteomics, and oleic acid rescue in mouse embryos

    PMID:41575276

    Open questions at the time
    • Whether the lipid phenotype is solely export-dependent versus additional roles unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How NCBP1's distinct partnerships (NCBP2 versus NCBP3, CTIF, UPF1, SF3A1, and m6A readers) are selected and switched on individual transcripts under different cellular and stress states remains unresolved.
  • No unified model of partner selection across export, translation, NMD, and splicing
  • Structural basis for context-dependent complex assembly unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 4 GO:0060090 molecular adaptor activity 4 GO:0140299 molecular sensor activity 1
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 3
Pathway
R-HSA-8953854 Metabolism of RNA 5 R-HSA-392499 Metabolism of proteins 2 R-HSA-74160 Gene expression (Transcription) 2 R-HSA-8953897 Cellular responses to stimuli 2 R-HSA-9609507 Protein localization 2
Partners
CTIFEIF3GNCBP2NCBP3SF3A1UPF1UPF2
Complex memberships
CBP80/20-dependent translation initiation complexSURF complexalternative cap-binding complex (NCBP1-NCBP3)nuclear cap-binding complex (CBC; NCBP1-NCBP2)

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 CBP80 (NCBP1) is associated with newly synthesized mRNAs undergoing a 'pioneer' round of translation; NMD targets CBP80-bound mRNAs (not yet replaced by eIF4E), and the NMD-susceptible mRNP includes CBP20, PABP2, eIF4G, Upf2, and Upf3 as components. NMD of CBP80-bound mRNA is blocked by cycloheximide or suppressor tRNA, confirming translation-dependence. Antibody immunopurification of CBP80-bound vs. eIF4E-bound mRNPs; cycloheximide and suppressor tRNA inhibition assays Cell High 11551508
2002 CBP80 (NCBP1), but not nuclear eIF4E, is detected in association with intron-containing RNA and the C-terminal domain of RNA polymerase II. The exon junction complex (EJC) components RNPS1, Y14, SRm160, REF/Aly, TAP, Upf3X, and Upf2 are detected on CBP80-bound mRNA in both nuclear and cytoplasmic fractions, but not on eIF4E-bound mRNA, indicating these proteins travel with CBP80-mRNPs after export. Immunoprecipitation of CBP80-bound vs. eIF4E-bound mRNPs; nuclear/cytoplasmic fractionation; Western blotting The EMBO journal High 12093754
1995 NCBP1 (CBP80) interacts with three nuclear cap-binding protein interacting proteins (NIP1, NIP2, NIP3) identified by yeast two-hybrid; NCBP1 requires NIP1 (which has an RNP-type RNA binding domain) for binding to the cap structure. Yeast two-hybrid screen from HeLa cell cDNA library; cap-binding assay Nucleic acids research Medium 7478990
2005 CBP80 (NCBP1) directly interacts with Upf1 and promotes the interaction of Upf1 with Upf2 during NMD, but does not promote Upf1 interaction with Stau1 (which mediates SMD). CBP80 augments the efficiency of NMD but not Staufen1-mediated mRNA decay (SMD). Co-immunoprecipitation; siRNA knockdown; NMD efficiency assays Nature structural & molecular biology High 16186820
2005 CBP80 (NCBP1) and eIF4G share a common evolutionary origin and similar domain organization (consecutive HEAT domains). A structural model based on the CBP80-CBP20 crystal structure suggests conserved mutual orientation of domains relevant for translation initiation complex assembly. Computational domain analysis; structural modeling using known CBP80-CBP20 complex structure Biochemistry Low 16156639
2009 CBP80 (NCBP1) directly interacts with CTIF (CBP80/20-dependent translation initiation factor), a new MIF4G domain-containing protein. CTIF is part of the CBP80/20-dependent translation initiation complex, and depletion of CTIF from an in vitro translation system selectively blocks translation of CBP80-bound mRNAs; addition of purified CTIF restores it. CTIF localizes to the perinuclear region, and its down-regulation abrogates NMD. Co-immunoprecipitation; in vitro translation system depletion/reconstitution; siRNA knockdown; confocal microscopy Genes & development High 19648179
2010 UPF1 binding to CBP80 (NCBP1) promotes NMD at two distinct steps: (1) association of SMG1 and UPF1 with eukaryotic release factors (eRFs) during SURF complex formation at a premature termination codon, and (2) subsequent association of SMG1 and UPF1 with an exon-junction complex. UPF1 binds PTC-containing mRNA more efficiently than PTC-free mRNA in a manner promoted by the UPF1-CBP80 interaction. Dominant-negative mutant precluding UPF1-CBP80 interaction; co-immunoprecipitation; mRNA binding assays; NMD reporter assays Molecular cell High 20691628
2009 CBP80 (NCBP1) is present in neuronal dendrites and associates with LSm1-mRNPs assembled in the nucleus; both LSm1 and CBP80 shift into dendritic spines upon stimulation of glutamatergic receptors, suggesting these CBP80-containing mRNPs are translationally activated during local protein synthesis. Immunofluorescence; subcellular fractionation; glutamatergic receptor stimulation; confocal microscopy The Journal of cell biology Medium 19188494
2012 CTIF (CBP80/20-dependent translation initiation factor) specifically interacts with eIF3g (a component of the eIF3 ribosome-recruitment complex), bridging CBP80 and eIF3 to enable ribosome recruitment during CBP80/20-dependent translation. Down-regulation of CTIF redistributes CBP80 from polysome to subpolysome fractions without affecting eIF4E distribution. Artificial tethering of CTIF to an intercistronic region drives translation of the downstream cistron in an eIF3-dependent manner. Co-immunoprecipitation; polysome fractionation; siRNA knockdown; tethering assay; NMD reporter assay The Journal of biological chemistry High 22493286
2015 NCBP1 (CBP80), but not NCBP2 (CBP20), is required for cell viability and poly(A) RNA nuclear export. NCBP1 forms an alternative cap-binding complex with NCBP3 (C17orf85) that binds mRNA, associates with mRNA processing machinery, and contributes to poly(A) RNA export. Loss of NCBP3 is compensated by NCBP2 under steady-state conditions but NCBP3 becomes critical under stress (e.g., virus infection). Knockdown/knockout viability assays; RNA export assays (FISH); mass spectrometry interactome; Co-immunoprecipitation Nature communications High 26382858
2008 NMD triggered by EMCV IRES-initiated translation targets CBP80/20-bound mRNA but does not detectably target eIF4E-bound mRNA, establishing that even IRES-initiated translation undergoes a CBP80-associated pioneer round leading to NMD when translation terminates prematurely. NMD reporter assays with EMCV IRES constructs; immunoprecipitation of CBP80-bound vs. eIF4E-bound mRNA fractions EMBO reports Medium 18369367
2011 In yeast, the CBP80 ortholog Cbc1/Sto1 associates with polysomes and is required for rapid translation reinitiation after osmotic stress. Deletion of CBC1 causes hypersensitivity to cycloheximide and synthetic sickness with limiting eIF4E. Osmostress-responsive mRNAs are transcriptionally induced in cbc1Δ cells but fail to rapidly associate with polysomes. Under osmotic stress, Cbc1 relocalizes from nucleus to cytoplasm. Polysome fractionation; genetic epistasis (cbc1Δ, eIF4E temperature-sensitive allele); cycloheximide sensitivity assay; live-cell localization Molecular biology of the cell High 22072789
2011 Ago2 competes with CBP80/20 for cap association, thereby inhibiting CBP80/20-dependent translation (CT) and abrogating NMD that is coupled to CT. Tethering of Ago2 (but not of cap-association-deficient Ago2F2V2) to the 3'UTR of PTC-containing mRNA abrogates NMD. Immunoprecipitation with CBP80 antibody confirms that Ago2, but not Ago2F2V2, inhibits binding of CBP80/20 to the cap structure. Tethering assay; co-immunoprecipitation with CBP80 antibody; NMD reporter assay; Ago2 mutant (F2V2) FEBS letters Medium 21840310
2018 CBP80 (NCBP1) binds the HIV-1 viral protein Rev and the unspliced full-length mRNA in both nucleus and cytoplasm. CBP80 supports Rev-mediated nuclear export and translation of the unspliced mRNA. Rev interacts with DEAD-box helicase eIF4AI, and the Rev/RRE axis is required for assembly of a CBP80-eIF4AI complex on the unspliced mRNA. Co-immunoprecipitation; RNA immunoprecipitation; translation and nuclear export assays; RIP Nucleic acids research Medium 30239828
2010 In yeast, Cbp80 (NCBP1 ortholog) plays distinct roles in splicing: it promotes initial 5' splice site recognition by U1 snRNP and, independently, facilitates U2 snRNP recruitment in a manner dependent on sequences near the 5' splice site. Deletion of Cbp80 suppresses the splicing defect caused by a mutation in the RPL30 binding site that normally disrupts L30-mediated splicing repression. Genetic epistasis (cbp80 deletion suppressor screen); splicing assays RNA (New York, N.Y.) Medium 20801768
2019 NCBP1 up-regulates CUL4B expression via interaction with NCBP3, constituting an NCBP1-NCBP3-CUL4B axis that promotes lung cancer cell growth. CUL4B silencing significantly reverses NCBP1-induced tumorigenesis in vitro. Co-immunoprecipitation; knockdown/overexpression; cell growth and migration assays; CUL4B rescue experiment Journal of cellular and molecular medicine Medium 31448526
2019 In Drosophila, Cbp80 (NCBP1 ortholog) cooperates with Paip2 at active promoters to ensure proper RNA polymerase II CTD Ser5 phosphorylation, linking the cap-binding complex to transcription initiation/early elongation. Co-immunoprecipitation; ChIP; Pol II CTD phosphorylation assay; ~300 kDa complex characterization FEBS letters Medium 31001806
2023 NCBP1 enhances the m6A catalytic function of METTL3 by maintaining METTL3 mRNA stabilization, leading to increased m6A modification of c-MYC mRNA and enhanced DLBCL cell proliferation via the NCBP1/METTL3/m6A/c-MYC axis. Co-immunoprecipitation; siRNA knockdown; m6A methylation assay; mRNA stability assay; cell proliferation assay Scientific reports Medium 37244946
2024 NCBP1 is recruited by IGF2BP3 to inhibit CDK6 mRNA decay; IGF2BP3 recognizes m6A modification at the GGACU motif (nucleotides 110-114) in the 5'UTR of CDK6 mRNA and recruits NCBP1 to enhance CDK6 mRNA stability, thereby inhibiting renal tubular senescence. Co-immunoprecipitation (IP-MS); m6A site mapping; mRNA stability assay; siRNA knockdown; overexpression rescue Translational research Medium 38945255
2026 NCBP1 propagates nuclear electrophile stress signals through a single cysteine residue (C436). Electrophile modification of NCBP1(C436) impairs association between NCBP1 and SF3A1 (an essential spliceosome component), triggering alternative splicing of >250 genes including S6 kinase, whose alternatively spliced isoform is sufficient to inhibit global protein translation. Precision localized electrophile generation; genetic code expansion; alternative splicing sequencing; Co-immunoprecipitation (NCBP1-SF3A1 interaction); site-directed mutagenesis (C436); polysome/translation assays Nature chemical biology High 41667655
2026 Ncbp1 depletion in mouse embryos causes morula arrest with nuclear poly(A) RNA retention and downregulation of lipid metabolic pathways, notably SCD1 (stearoyl-CoA desaturase 1). Exogenous oleic acid supplementation partially rescues blastocyst formation, implicating NCBP1 in SCD1-OA-mediated lipid metabolic homeostasis during morula-to-blastocyst transition via its role in mRNA export. siRNA knockdown via zygotic microinjection; mRNA rescue (co-injection); poly(A) RNA FISH; RNA sequencing; quantitative proteomics; oleic acid rescue experiment Reproduction (Cambridge, England) Medium 41575276
2020 CTIF inhibits HIV-1 Gag synthesis by associating with HIV-1 Rev through its N-terminal domain and being recruited onto the full-length RNA RNP complex. CTIF induces cytoplasmic accumulation of Rev, impeding Rev association with CBP80. Rev and CTIF compete for binding to CBP80, establishing a regulatory interplay between the CBC-dependent translation machinery and HIV-1 replication. Co-immunoprecipitation; subcellular fractionation; siRNA knockdown; translation reporter assays RNA biology Medium 33103564
2017 In Drosophila, Cbp80 (NCBP1 ortholog) knockdown in the female germline leads to delocalization and reduced protein levels of the piRNA pathway factors Piwi, Aub, and Ago3, and impairs both primary piRNA biogenesis and the ping-pong secondary amplification cycle, without significantly altering piRNA precursor transcript levels or nuage localization. Germline-specific RNAi knockdown; small RNA sequencing; immunofluorescence for piRNA pathway factors PloS one Medium 28746365

Source papers

Stage 0 corpus · 33 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20. Cell 491 11551508
2002 The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling. The EMBO journal 221 12093754
2018 Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence. Plant biotechnology journal 176 30019807
2008 Two cap-binding proteins CBP20 and CBP80 are involved in processing primary MicroRNAs. Plant & cell physiology 138 18829588
2005 CBP80 promotes interaction of Upf1 with Upf2 during nonsense-mediated mRNA decay in mammalian cells. Nature structural & molecular biology 126 16186820
2009 A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation. Genes & development 101 19648179
2010 UPF1 association with the cap-binding protein, CBP80, promotes nonsense-mediated mRNA decay at two distinct steps. Molecular cell 97 20691628
2015 mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3. Nature communications 94 26382858
2005 eIF4G and CBP80 share a common origin and similar domain organization: implications for the structure and function of eIF4G. Biochemistry 49 16156639
1995 Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein. Nucleic acids research 49 7478990
2012 Translation initiation on mRNAs bound by nuclear cap-binding protein complex CBP80/20 requires interaction between CBP80/20-dependent translation initiation factor and eukaryotic translation initiation factor 3g. The Journal of biological chemistry 45 22493286
2009 Dendritic LSm1/CBP80-mRNPs mark the early steps of transport commitment and translational control. The Journal of cell biology 45 19188494
2011 CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Cold Spring Harbor symposia on quantitative biology 44 21447822
2011 Yeast mRNA cap-binding protein Cbc1/Sto1 is necessary for the rapid reprogramming of translation after hyperosmotic shock. Molecular biology of the cell 44 22072789
2019 NCBP1 promotes the development of lung adenocarcinoma through up-regulation of CUL4B. Journal of cellular and molecular medicine 36 31448526
2018 A Rev-CBP80-eIF4AI complex drives Gag synthesis from the HIV-1 unspliced mRNA. Nucleic acids research 24 30239828
2008 NMD resulting from encephalomyocarditis virus IRES-directed translation initiation seems to be restricted to CBP80/20-bound mRNA. EMBO reports 21 18369367
2011 Ago2/miRISC-mediated inhibition of CBP80/20-dependent translation and thereby abrogation of nonsense-mediated mRNA decay require the cap-associating activity of Ago2. FEBS letters 18 21840310
2007 Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia. FEBS letters 15 17942097
2019 Paip2 cooperates with Cbp80 at an active promoter and participates in RNA Polymerase II phosphorylation in Drosophila. FEBS letters 12 31001806
2010 RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment. RNA (New York, N.Y.) 10 20801768
2020 CBP80/20-dependent translation initiation factor (CTIF) inhibits HIV-1 Gag synthesis by targeting the function of the viral protein Rev. RNA biology 9 33103564
2023 NCBP1 enhanced proliferation of DLBCL cells via METTL3-mediated m6A modification of c-Myc. Scientific reports 6 37244946
2022 Eukaryotic translation initiation factor 4E family member nCBP facilitates the accumulation of TGB-encoding viruses by recognizing the viral coat protein in potato and tobacco. Frontiers in plant science 6 36003826
2024 IGF2BP3/NCBP1 complex inhibits renal tubular senescence through regulation of CDK6 mRNA stability. Translational research : the journal of laboratory and clinical medicine 5 38945255
2023 NCBP1 Improves Cognitive Function in Mice by Reducing Oxidative Stress, Neuronal Loss, and Glial Activation After Status Epilepticus. Molecular neurobiology 5 37474884
2020 Transcriptional Coactivator PGC-1α Binding to Newly Synthesized RNA via CBP80: A Nexus for Co- and Posttranscriptional Gene Regulation. Cold Spring Harbor symposia on quantitative biology 5 32295928
2017 Cbp80 is needed for the expression of piRNA components and piRNAs. PloS one 2 28746365
2026 Identification of stomatin-1 (STO-1) as a novel arginine monomethylated protein in Caenorhabditis elegans. Bioscience, biotechnology, and biochemistry 1 41247075
2026 NCBP1 stress signaling drives alternative S6K1 splicing inhibiting translation. Nature chemical biology 1 41667655
2025 CRISPR/Cas9 editing of CBP80 enhances drought tolerance in potato (Solanum tuberosum). Frontiers in plant science 1 40708586
2026 Ncbp1 deficiency affects morula-to-blastocyst transition through lipid metabolic dysregulation. Reproduction (Cambridge, England) 0 41575276
2010 CBP80 choreographs the NMD two-step. Molecular cell 0 20705234

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