Affinage

CALB1

Calbindin · UniProt P05937

Length
261 aa
Mass
30.0 kDa
Annotated
2026-04-28
82 papers in source corpus 9 papers cited in narrative 13 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CALB1 (calbindin D28k) is an intracellular calcium-binding protein that buffers cytoplasmic Ca²⁺ and maintains calcium homeostasis in the endoplasmic reticulum and mitochondria, thereby protecting cells from calcium overload–induced mitochondrial dysfunction, oxidative stress, and senescence (PMID:36012633, PMID:39748132, PMID:40902499). CALB1 is upregulated during senescence through the calcineurin/NFAT pathway and interacts directly with MDM2 to promote p53–MDM2 association, suppressing p53-dependent senescence in cancer cells (PMID:36012633, PMID:31059057). In the nervous system, CALB1 marks functionally specialized neuronal subpopulations—including prefrontal cortex layer III pyramidal neurons where it colocalizes with L-type calcium channels, NMDA receptors, and SK3 potassium channels at dendritic spines adjacent to calcium-storing smooth ER, forming a calcium-regulatory hub essential for working memory–related persistent firing (PMID:38776078). CALB1 expression is regulated downstream of FGF10–FGFR2 signaling in hippocampal neurons, where its restoration confers neuroprotection in epilepsy models (PMID:41121240).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 1991 Medium

    Establishing the chromosomal location of human CALB1 placed this calcium-binding protein gene at 8q21.3–q22.1 and linked it to the carbonic anhydrase gene cluster, suggesting an ancestral duplication origin.

    Evidence In situ hybridization chromosomal mapping

    PMID:1906795

    Open questions at the time
    • Functional significance of the co-localization with carbonic anhydrase genes was not investigated
    • No regulatory element characterization was performed
  2. 2019 Medium

    The discovery that CALB1 directly binds MDM2 and promotes p53–MDM2 interaction revealed an unexpected non-calcium-buffering function: suppression of p53-dependent senescence in ovarian cancer cells.

    Evidence Co-immunoprecipitation, pull-down assay, senescence assays, and growth assays in ovarian cancer cell lines

    PMID:31059057

    Open questions at the time
    • Whether the CALB1–MDM2 interaction is calcium-dependent was not determined
    • No structural basis for the CALB1–MDM2 interaction
    • Not independently replicated in a second lab
  3. 2021 Medium

    Identification of a MIAT/miR-34b-5p/CALB1 regulatory axis in hippocampal neurons defined an upstream mechanism controlling CALB1 expression and linked CALB1 to neuroprotection against apoptosis.

    Evidence Dual-luciferase reporter assay validating miR-34b-5p targeting of CALB1 mRNA, functional viability and apoptosis assays in hippocampal neuronal cells

    PMID:34650682

    Open questions at the time
    • In vivo validation of this regulatory axis was limited
    • Whether this axis operates in non-hippocampal neurons is unknown
  4. 2022 Medium

    Demonstrating that CALB1 is transcriptionally upregulated via the calcineurin/NFAT pathway during senescence and that it buffers the elevated intracellular Ca²⁺ of senescent cells established CALB1 as a feedback regulator of calcium homeostasis in the senescence program.

    Evidence Live-cell calcium imaging, overexpression/knockdown, calcineurin/NFAT pathway inhibitors in human mammary epithelial cells subjected to multiple senescence inducers

    PMID:36012633

    Open questions at the time
    • Whether CALB1 delays or promotes senescence execution was not fully resolved
    • In vivo relevance of this feedback loop in aging tissues not tested
  5. 2024 High

    Showing that CALB1-expressing primate prefrontal layer III pyramidal neurons co-express Cav1.2, GluN2B, and SK3 at dendritic spines near calcium-storing smooth ER, and that L-type channel activity is required for persistent firing during working memory, established CALB1+ neurons as a molecularly defined calcium-regulatory hub in prefrontal circuits.

    Evidence Transcriptomics, immunoelectron microscopy, in vivo neuronal recordings during spatial working memory, and pharmacological manipulations in macaques

    PMID:38776078

    Open questions at the time
    • Whether CALB1 calcium buffering is causally required for working memory firing was not directly tested (CALB1 served as a cell-type marker)
    • Mechanism by which CALB1 modulates calcium dynamics in these spines not resolved at the biophysical level
  6. 2025 Medium

    Loss-of-function and rescue experiments in oocytes and cancer cells converged to show that CALB1 maintains ER and mitochondrial calcium stores; its depletion causes calcium overload, mitochondrial dysfunction, oxidative stress, and either meiotic defects or senescence depending on cell type.

    Evidence siRNA knockdown and overexpression in mouse oocytes with calcium and mitochondrial function measurements (PMID:39748132); CALB1 knockdown with calcium chelation and mitochondrial rescue in prostate cancer cells and xenografts (PMID:40902499)

    PMID:39748132 PMID:40902499

    Open questions at the time
    • Direct calcium-binding stoichiometry and kinetics of CALB1 in these cellular compartments not measured
    • Whether CALB1 localizes to ER/mitochondrial contact sites or acts indirectly is unresolved
    • Each study from a single lab
  7. 2025 Medium

    Placing CALB1 downstream of FGF10–FGFR2 signaling in hippocampal dentate gyrus neurons showed that its expression is regulated by receptor tyrosine kinase pathways and contributes to neuroprotection during epilepsy.

    Evidence KA-induced epilepsy model, intranasal FGF10, FGFR2 conditional knockout epistasis, RNA-seq, EEG, and behavioral tests in mice

    PMID:41121240

    Open questions at the time
    • Whether CALB1 is a direct or indirect transcriptional target of FGFR2 signaling not determined
    • Sufficiency of CALB1 restoration alone for neuroprotection not tested independently of FGF10
  8. 2025 Medium

    Intersectional genetic studies in mouse midbrain and hypothalamus used CALB1 as a marker to reveal functional specialization of CALB1+ neuronal populations in motor control, shivering thermogenesis, and reproductive behavior, though these studies addressed CALB1+ cell identity rather than the protein's molecular mechanism. (preprints)

    Evidence Intersectional ablation, chemogenetics, optogenetics, in vivo calcium imaging, and electrophysiology in Calb1-Cre mouse lines (preprints)

    Open questions at the time
    • All three studies are preprints awaiting peer review
    • CALB1 was used as a cell-type marker; whether its calcium-buffering activity is mechanistically required in these circuits was not tested
    • Molecular partners of CALB1 in these neuronal subtypes remain unidentified

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown whether CALB1's calcium-buffering function is causally required in the specific neuronal subtypes it marks, what structural determinants govern its interaction with MDM2, and whether ER/mitochondrial calcium maintenance by CALB1 involves direct organellar targeting or indirect cytoplasmic buffering.
  • No structural model of CALB1–MDM2 complex exists
  • Subcellular targeting mechanism of CALB1 to ER and mitochondrial calcium stores unresolved
  • Causal requirement for CALB1 protein (vs. marker status) in specialized neuronal circuits not directly tested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 3
Localization
GO:0005829 cytosol 3 GO:0005739 mitochondrion 2 GO:0005783 endoplasmic reticulum 2
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-382551 Transport of small molecules 3 R-HSA-5357801 Programmed Cell Death 2
Partners
CACNA1CGRIN2BKCNN3MDM2TP53

Evidence

Reading pass · 13 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 CALB1 protein interacts directly with MDM2 proto-oncogene (shown by co-immunoprecipitation and pull-down assays) and promotes the interaction between p53 and MDM2, thereby suppressing the p53 pathway and inhibiting cellular senescence in ovarian cancer cells. Co-immunoprecipitation, pull-down assay, MTT assay, anchorage-independent growth assay, senescence assay, western blot, immunohistochemistry Molecular medicine reports Medium 31059057
2022 CALB1 expression is upregulated in senescent cells through the Ca2+-dependent calcineurin/NFAT pathway, and overexpression of CALB1 buffers the rise in intracellular Ca2+ levels observed in senescent cells, establishing CALB1 as a calcium buffer that modulates intracellular Ca2+ during cellular senescence. Live-cell calcium measurements, overexpression/knockdown experiments in immortalized human mammary epithelial cells, pathway inhibitor analysis (calcineurin/NFAT), multiple senescence inducers International journal of molecular sciences Medium 36012633
2025 Knockdown of CALB1 in mouse oocytes leads to reduced calcium ion levels in the endoplasmic reticulum and mitochondria, resulting in mitochondrial dysfunction and meiotic defects; overexpression of CALB1 in aging oocytes partially rescues age-related defective phenotypes, establishing CALB1 as a regulator of calcium homeostasis essential for oocyte quality. Single-cell transcriptome sequencing, siRNA knockdown, overexpression in oocytes, calcium ion measurement, mitochondrial function assays, spindle assembly analysis Aging cell Medium 39748132
2025 CALB1 silencing in prostate cancer cells disrupts calcium homeostasis, induces calcium overload, and causes mitochondrial dysfunction and oxidative stress, leading to cellular senescence and enhanced radiosensitivity; these effects are partially rescued by calcium chelation or mitochondrial interventions, placing CALB1 upstream of calcium-mediated mitochondrial pathways in radiation response. CALB1 knockdown, calcium chelation, mitochondrial rescue interventions, xenograft models, proliferation and senescence assays, miR-186-5p regulatory validation Cell calcium Medium 40902499
1991 The human CALB1 gene encoding calbindin D28k was mapped by in situ hybridization to chromosomal region 8q21.3–q22.1, colocalizing with the carbonic anhydrase isozyme gene cluster (CA1, CA2, CA3), suggesting a common ancestral duplication event. In situ hybridization chromosomal mapping Cytogenetics and cell genetics Medium 1906795
2024 In primate dorsolateral prefrontal cortex layer III pyramidal neurons, CALB1 (calbindin) is selectively coexpressed with CACNA1C (Cav1.2 L-type calcium channels), GRIN2B (GluN2B-NMDA receptors), and KCNN3 (SK3 potassium channels) concentrated in dendritic spines near the calcium-storing smooth endoplasmic reticulum; L-type calcium channel blockade or excessive activation both reduced neuronal firing needed for working memory, with the latter acting via SK potassium channel opening, indicating that CALB1-expressing neurons form a distinct calcium-regulatory hub in prefrontal circuits. Transcriptomic analysis, protein expression by light and electron microscopy, in vivo neuronal firing recordings during spatial working memory task, pharmacological manipulations in macaques JAMA psychiatry High 38776078
2021 MiR-34b-5p directly targets both MIAT (lncRNA) and CALB1 mRNA (validated by dual-luciferase assay), and MIAT-containing exosomes from hippocampal neural stem cells upregulate CALB1 expression in hippocampal neuronal cells, with CALB1 overexpression increasing cell viability and reducing apoptosis, establishing a MIAT/miR-34b-5p/CALB1 regulatory axis. Dual-luciferase reporter assay, western blot, CCK-8 viability assay, Annexin V-FITC/PI apoptosis assay, qRT-PCR, Morris water maze American journal of translational research Medium 34650682
2023 CALB1 protein isolated from chicken ileal mucus suppresses avian virus replication, possibly by binding calcium ions and/or inducing autophagy, as demonstrated by experiments with both eukaryotically and prokaryotically expressed recombinant CALB1. Protein isolation from intestinal mucus, LC-MS identification, recombinant protein expression (eukaryotic and prokaryotic), viral replication assays International journal of biological macromolecules Low 37734520
2025 CALB1-expressing (Calb1+) neurons in the dorsomedial hypothalamus that are also VGLUT2-positive selectively initiate cold-evoked shivering thermogenesis; their cold-dependent firing relies on TRPM8 receptor activation, and knockdown of TRPM8 in these neurons reduces cold-evoked shivering in vivo. RNA sequencing of retrogradely labeled neurons, Cre-dependent chemogenetics, optogenetics, in vivo electrophysiology, TRPM8 knockdown, pharmacological TRPM8 activation/inhibition, c-Fos mapping bioRxivpreprint Medium
2024 CALB1-expressing (Calb1+) neurons in the preoptic area of the hypothalamus are specifically activated during ejaculation in male mice; inhibiting these neurons prolongs mating and delays ejaculation, and they transmit the ejaculation signal to activate POMC-expressing neurons in the arcuate nucleus, which drives β-endorphin release post-ejaculation. In vivo calcium imaging, chemogenetic inhibition, optogenetic activation, β-endorphin measurement, conditioned place preference, intracerebroventricular drug infusion bioRxivpreprint Medium
2025 Selective ablation of CALB1+ dopaminergic neurons in the mouse midbrain impairs initiation and vigor of voluntary movements and disrupts retention of acquired motor skills; conversely, ablation of CALB1- dopaminergic neurons disrupts locomotor learning acquisition, demonstrating functional specialization of CALB1+ vs CALB1- midbrain DA neuron subtypes in motor control. Intersectional genetic ablation, chemogenetic inhibition (DREADD hM4Di), locomotor behavioral assays, immunohistochemistry bioRxivpreprint Medium
2025 Selective chemogenetic inhibition of Calb1+ nigrostriatal dopaminergic neurons impairs voluntary movement, motor skill learning retention, and early associative learning behavior, while Aldh1a1+ DAN inhibition also impairs movement and motor learning but not associative learning, establishing Calb1+ neurons as regulators of both motor and reward-based associative learning. Intersectional genetics with AAV-CreOn-FlpOn-hM4Di delivery, DREADD chemogenetic inhibition in Th-Flp;Calb1-IRESCre double knock-in mice, behavioral assays bioRxivpreprint Medium
2025 FGF10 treatment restores decreased CALB1 levels in the hippocampal dentate gyrus of epileptic mice and exerts neuroprotective effects; this action is abolished in FGFR2 conditional knockout mice, placing CALB1 downstream of FGF10-FGFR2 signaling in neuronal protection during epilepsy. KA-induced epilepsy mouse model, intranasal FGF10 administration, AAV overexpression, FGFR2 conditional knockout, RNA sequencing, Western blotting, qRT-PCR, EEG monitoring, behavioral tests Journal of translational medicine Medium 41121240

Source papers

Stage 0 corpus · 82 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Ceramide binds to the CaLB domain of cytosolic phospholipase A2 and facilitates its membrane docking and arachidonic acid release. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 110 11099485
2010 Selection of CalB immobilization method to be used in continuous oil transesterification: analysis of the economical impact. Enzyme and microbial technology 61 22112772
2017 Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide. Biotechnology reports (Amsterdam, Netherlands) 44 28459004
1995 CaLB: a 43 amino acid calcium-dependent membrane/phospholipid binding domain in p120 Ras GTPase-activating protein. Oncogene 42 7898923
1991 The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3----q22.1 and 16q22----q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci. Cytogenetics and cell genetics 30 1906795
2024 Key Roles of CACNA1C/Cav1.2 and CALB1/Calbindin in Prefrontal Neurons Altered in Cognitive Disorders. JAMA psychiatry 29 38776078
2012 Production of flavor esters catalyzed by CALB-displaying Pichia pastoris whole-cells in a batch reactor. Journal of biotechnology 29 22410080
2021 HNSC exosome-derived MIAT improves cognitive disorders in rats with vascular dementia via the miR-34b-5p/CALB1 axis. American journal of translational research 26 34650682
2019 CALB1 enhances the interaction between p53 and MDM2, and inhibits the senescence of ovarian cancer cells. Molecular medicine reports 26 31059057
1995 Mutation-deletion analysis of a Ca(2+)-dependent phospholipid binding (CaLB) domain within p120 GAP, a GTPase-activating protein for p21 ras. The Biochemical journal 26 7733887
2014 Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALB. Molecules (Basel, Switzerland) 24 25004067
2013 AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15(+)/CalB(+) leukocytes. PloS one 24 24086629
2015 Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate. Bioprocess and biosystems engineering 23 26037641
2014 Immobilization and stabilization of lipase (CaLB) through hierarchical interfacial assembly. Biomacromolecules 22 25252004
2022 Expression of the Calcium-Binding Protein CALB1 Is Induced and Controls Intracellular Ca2+ Levels in Senescent Cells. International journal of molecular sciences 21 36012633
2019 CALB Immobilized onto Magnetic Nanoparticles for Efficient Kinetic Resolution of Racemic Secondary Alcohols: Long-Term Stability and Reusability. Molecules (Basel, Switzerland) 21 30704049
2016 Stabilization of Candida antarctica Lipase B (CALB) Immobilized on Octyl Agarose by Treatment with Polyethyleneimine (PEI). Molecules (Basel, Switzerland) 21 27338317
2021 Brain CHID1 Expression Correlates with NRGN and CALB1 in Healthy Subjects and AD Patients. Cells 20 33924468
2009 Efficient synthesis of enantiomeric ethyl lactate by Candida antarctica lipase B (CALB)-displaying yeasts. Applied microbiology and biotechnology 20 19288094
2018 Structural characterization and immunomodulatory activity of a pectic polysaccharide (CALB-4) from Fructus aurantii. International journal of biological macromolecules 18 29409765
2010 Kinetic resolution of (R/S)-propranolol (1-isopropylamino-3-(1-naphtoxy)-2-propanolol) catalyzed by immobilized preparations of Candida antarctica lipase B (CAL-B). New biotechnology 18 20667519
2017 Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter. Applied microbiology and biotechnology 17 28130631
2019 Chemoenzymatic Dynamic Kinetic Resolution of Primary Benzylic Amines using Pd0 -CalB CLEA as a Biohybrid Catalyst. Chemistry (Weinheim an der Bergstrasse, Germany) 16 31050831
2010 Cell-free synthesis and multifold screening of Candida antarctica lipase B (CalB) variants after combinatorial mutagenesis of hot spots. Biotechnology progress 16 21312354
2000 Molecular characterization of a calmodulin-like dictyostelium protein CalB. FEBS letters 16 10818233
2016 Quantification of immobilized Candida antarctica lipase B (CALB) using ICP-AES combined with Bradford method. Enzyme and microbial technology 15 28010778
2017 Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and adenylate kinase. PloS one 14 28715469
2016 CaLB Catalyzed Conversion of ε-Caprolactone in Aqueous Medium. Part 1: Immobilization of CaLB to Microgels. Polymers 14 30974648
2024 Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation. Nature communications 13 38898014
1999 Investigating the role played by protein-lipid and protein-protein interactions in the membrane association of the p120GAP CaLB domain. Cellular signalling 13 10400317
2021 Reviewing research on the synthesis of CALB-catalyzed sugar esters incorporating systematic mapping principles. Critical reviews in biotechnology 12 33645353
2008 Functional fusion mutant of Candida antarctica lipase B (CalB) expressed in Escherichia coli. Biochimica et biophysica acta 12 19159700
2022 Synthesis of organic-inorganic hybrid nanoflowers of lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL): Improvement of thermal stability and reusability. Enzyme and microbial technology 11 36493680
2014 Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity. Applied microbiology and biotechnology 10 24469105
2022 Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure. Scientific reports 9 35882890
2017 Strep-tag II fusion technology for the modification and immobilization of lipase B from Candida antarctica (CALB). Journal, genetic engineering & biotechnology 8 30647674
2009 Recognition of acyl donors by lipase CAL-B in the acylation of 6-azauridine. Biotechnology progress 8 19452525
2019 Dissecting the evolvability landscape of the CalB active site toward aromatic substrates. Scientific reports 7 31666622
2015 Scaling-up the synthesis of myristate glucose ester catalyzed by a CALB-displaying Pichia pastoris whole-cell biocatalyst. Enzyme and microbial technology 7 26047913
2025 Immobilized Candida antarctica lipase B (CALB) for biodiesel production from rapeseed oil; evaluation of the effect of immobilization protocol. International journal of biological macromolecules 6 39809399
2022 Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification. International journal of molecular sciences 6 35269598
2022 Sortase-mediated immobilization of Candida antarctica lipase B (CalB) on graphene oxide; comparison with chemical approach. Biotechnology reports (Amsterdam, Netherlands) 6 35686009
2022 Atomevo: a web server combining protein modelling, docking, molecular dynamic simulation and MMPBSA analysis of Candida antarctica lipase B (CalB) fusion protein. Bioresources and bioprocessing 6 38647745
2019 Fast Degradation of Bisphenol A in Water by Nanostructured CuNPs@CALB Biohybrid Catalysts. Nanomaterials (Basel, Switzerland) 6 31861354
2025 CALB1 and RPL23 Are Essential for Maintaining Oocyte Quality and Function During Aging. Aging cell 5 39748132
2025 The segregation of Calb1, Calb2, and Prph neurons reveals distinct and mixed neuronal populations and projections to hair cells in the inner ear and central nuclei. Developmental dynamics : an official publication of the American Association of Anatomists 5 41165193
2023 Thermally Stable and Reusable Silica and Nano-Fructosome Encapsulated CalB Enzyme Particles for Rapid Enzymatic Hydrolysis and Acylation. International journal of molecular sciences 5 37372985
2023 Do they make a good match? Molecular dynamics studies on CALB-catalyzed esterification of 3-phenylpropionic and cinnamic acids. Archives of biochemistry and biophysics 5 37923242
2022 The role of conformational dynamics in the activity of polymer-conjugated CalB in organic solvents. Physical chemistry chemical physics : PCCP 5 36069567
2021 A mild and chemoselective CALB biocatalysed synthesis of sulfoxides exploiting the dual role of AcOEt as solvent and reagent. Organic & biomolecular chemistry 5 33179689
2021 Encapsulation of CALB by nucleotide/metal ions coordination nanoparticles: highly selective catalysis of esterification while poor performance in glycerolysis reaction. Journal of the science of food and agriculture 5 34460944
2021 Molecular Mechanism of Methanol Inhibition in CALB-Catalyzed Alcoholysis: Analyzing Molecular Dynamics Simulations by a Markov State Model. Journal of chemical theory and computation 5 34494846
2015 Is Promiscuous CALB a Good Scaffold for Designing New Epoxidases? Molecules (Basel, Switzerland) 5 26404218
2022 Immobilization of Lipase from Candida antarctica B (CALB) by Sol-Gel Technique Using Rice Husk Ash as Silic Source and Ionic Liquid as Additive. Applied biochemistry and biotechnology 4 35907063
2023 CALB1: Anovel antiviral factor in chicken ileal mucus. International journal of biological macromolecules 3 37734520
2021 In Situ Calb Enzyme Immobilization in Mesoporous Material Type MCM-48 Synthesis Using Ionic Solid [C14MI]Cl as Structure-Directing Agent. Applied biochemistry and biotechnology 3 34524635
2013 CAL-B catalyzed desymmetrization of 3-alkylglutarate: "olefin effect" and asymmetric synthesis of pregabalin. Organic & biomolecular chemistry 3 23525234
2009 Ester synthesis reaction with CALB displaying yeast whole cell biocatalyst: effect of organic solvent and initial water content. Journal of bioscience and bioengineering 3 19804858
2025 Comparative Study of Enzymatic Lipolysis Using Nanofructosome-Coated CalB Lipase Encapsulated in Silica and Immobilized on Silica-Coated Magnetic Nanoparticles. ACS omega 2 40224481
2025 Cell surface display of CAL-B in Escherichia coli using the split GFP system. Enzyme and microbial technology 2 40752234
2024 Long chain capsaicin analogues synthetized by CALB-CLEAs show cytotoxicity on glioblastoma cell lines. Applied microbiology and biotechnology 2 38217255
2013 In silico screening of 393 mutants facilitates enzyme engineering of amidase activity in CalB. PeerJ 2 24010022
2024 Unveiling six novel CALB-like lipases using genome-centric and patent-driven prospection. Enzyme and microbial technology 1 39405779
2023 Novel CaLB-like Lipase Found Using ProspectBIO, a Software for Genome-Based Bioprospection. Biotech (Basel (Switzerland)) 1 36648832
2023 Preparation of Coupling Catalyst HamZIF-90@Pd@CALB with Tunable Hollow Structure for Efficient Dynamic Kinetic Resolution of 1-Phenylethylamine. Molecules (Basel, Switzerland) 1 36770588
2023 Biosynthesis of diisooctyl 2,5-furandicarboxylate by Candida antarctica lipase B (CALB) immobilized on a macroporous epoxy resin. Biotechnology and applied biochemistry 1 37264706
2023 Membrane-specific and calcium-dependent binding of the Arabidopsis C2 domain protein CaLB revealed by ATR-FTIR spectroscopy. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 1 37995652
2023 Theoretical calculation on degradation mechanism of novel copolyesters under CALB enzyme. Journal of environmental sciences (China) 1 39181639
2017 CALB-Catalyzed Two-Step Alcoholytic Desymmetrization of 3-Methylglutaric Diazolides in MTBE. Applied biochemistry and biotechnology 1 29243042
2009 [Synthesis of flavor esters catalyzed by CALB-displaying Pichia pastoris whole-cells in non-aqueous phase]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology 1 20352970
2008 [Expression of Candida antarctica lipase B on yeast surface and synthesis of ethyl hexanoate catalyzed by CALB]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology 1 18616181
1999 A polymorphic CA repeat marker at the human 27-kD calbindin (CALB1) locus. Journal of human genetics 1 10570916
2026 Intramolecular Interactions of Twenty-Eight Amide Derivatives with the C-ALB Kinase using a Theoretical Model as a Therapeutic Alternative to Treat Cancer. Drug research 0 41633373
2025 A dataset for large prismatic lithium-ion battery cells (CALB L148N58A): Comprehensive characterization and real-world driving cycles. Data in brief 0 39911271
2025 ActiMut-XGB: Predicting thermodynamic stability of point mutations for CALB with protein language model. International journal of biological macromolecules 0 40414395
2025 Ethyl lactate synthesis in organic media using a magnetic supported CALB. Enzyme and microbial technology 0 40812212
2025 Silencing CALB1 enhances prostate cancer radiosensitivity via calcium-mediated mitochondrial dysfunction and cellular senescence. Cell calcium 0 40902499
2025 Co-immobilization of lipase from Thermomyces lanuginosus (TLL) and lipase B from Candida antarctica (CALB) on functionalized epoxy resin for enhanced stability, reusability, and biocatalytic efficiency. International journal of biological macromolecules 0 41057063
2025 FGF10 ameliorates epileptic seizures and related cognitive dysfunction via the FGFR2/CALB1 signaling pathway. Journal of translational medicine 0 41121240
2025 A CALB-like Cold-Active Lipolytic Enzyme from Pseudonocardia antarctica: Expression, Biochemical Characterization, and AlphaFold-Guided Dynamics. Marine drugs 0 41440913
2025 Solid/Gas Synthesis of Isobutyl Propionate Catalyzed by Packed-Bed CalB Cross-Linked Enzyme Aggregates (CLEA). ACS omega 0 41502762
2024 Molecular insight into the enhanced performance of CALB toward PBDF degradation. International journal of biological macromolecules 0 38360240