Affinage

Showing ART1 — ARTC1 is a alias.

ART1

GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1 · UniProt P52961

Length
327 aa
Mass
36.3 kDa
Annotated
2026-06-09
60 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge faithfulness: 6/7 claims corpus-supported (86%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ART1/ARTC1 is a GPI-anchored, arginine-specific ecto-ADP-ribosyltransferase, predominantly expressed in cardiac and skeletal muscle, that transfers ADP-ribose from extracellular NAD+ onto arginine residues of cell-surface and extracellular target proteins (PMID:9841866, PMID:30110646). In muscle tissue it carries out widespread arginine ADP-ribosylation of hundreds of extracellular and surface proteins, including the validated substrate hemopexin, modifying targets linked to signal transduction, transmembrane transport, and muscle function (PMID:30110646). Muscle-restricted ARTC1 expression is driven by cooperative binding of myogenin to an E-box and MEF-2 to an A/T-rich element in the proximal promoter (PMID:18939989). The closely related ARTC1-family ectoenzyme ART2 ADP-ribosylates the P2X7 purinoceptor on T cells, activating it to trigger calcium flux, pore formation, phosphatidylserine exposure, and NAD-induced cell death (NICD), a pathway abolished in ART2-deficient T cells (PMID:14563321, PMID:12370300); in vivo this axis depletes naive T cells during inflammation and depletes P2X7-high regulatory T cells, with Treg depletion promoting antitumor responses (PMID:17579037, PMID:20975043). On tumor cells, ART1 itself ADP-ribosylates P2X7R+ CD8 T cells to drive their NICD and exclude them from tumors, constituting an immune-evasion mechanism whose genetic or antibody blockade slows tumor growth in a CD8-dependent manner (PMID:35294260). Enzyme activity and substrate specificity are controlled by GPI-anchor-dependent lipid-raft association, which restricts substrate range and supports auto-ADP-ribosylation, and by metalloprotease-mediated ectodomain shedding of active enzyme from the cell surface (PMID:15657180, PMID:11035085). A subset of human ARTC1 localizes atypically to the endoplasmic reticulum where it ADP-ribosylates the chaperone GRP78/BiP during ER stress (PMID:25292337), and it modifies VAPB at Arg50 to influence calcium homeostasis, with hARTC3 binding and stabilizing ARTC1 to promote its activity (PMID:37381178).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1998 Medium

    Established the molecular identity of the gene product: that mouse Art1 is a GPI-anchored ecto-enzyme with intrinsic arginine-specific ADP-ribosyltransferase activity expressed in muscle.

    Evidence Northern/RT-PCR expression profiling and recombinant IgG1-Fc fusion enzymatic activity assay in 293T cells

    PMID:9841866

    Open questions at the time
    • In vitro substrates not physiological
    • No structure or active-site mapping
    • Muscle function of the enzyme not addressed
  2. 2002 High

    Resolved the catalytic architecture and demonstrated, genetically, that family ectoenzymes are required for NAD-mediated T cell regulation.

    Evidence 1.7 Å crystal structure of rat ART2.2 with nicotinamide-analogue ligand, plus ART2.1/2.2 double-KO T cell apoptosis and proliferation assays

    PMID:12270706 PMID:12370300

    Open questions at the time
    • Structure is of ART2.2 ortholog, not ART1 itself
    • Surface substrate driving T cell death not yet identified at this step
  3. 2003 High

    Identified the effector substrate of NAD-induced T cell death, defining the ART2–P2X7 axis as the mechanism converting extracellular NAD+ into a death signal.

    Evidence Flow cytometry, calcium flux, PI uptake, pharmacological inhibition, and ART2-deficient T cells

    PMID:14563321

    Open questions at the time
    • ADP-ribosylated arginine site on P2X7 not mapped here
    • Did not address ART1 enzyme specifically
  4. 2005 High

    Defined how GPI-anchor-dependent lipid raft localization tunes enzyme activity and narrows substrate specificity.

    Evidence GPI- vs transmembrane-anchored ART2.2 transfectants with cholesterol depletion and activity assays

    PMID:15657180

    Open questions at the time
    • Raft composition mediating specificity not defined
    • Generalization to ART1 in muscle not shown
  5. 2000 Medium

    Showed enzyme abundance at the cell surface is dynamically regulated by metalloprotease-mediated shedding, decoupling enzyme location from activity.

    Evidence FACS ADP-ribosylation assay with metalloprotease inhibitor and in vitro activity of shed enzyme

    PMID:11035085

    Open questions at the time
    • Specific protease not genetically confirmed
    • Physiological consequence of shed soluble enzyme unclear
  6. 2006 High

    Revealed catalytic versatility — poly(ADP-ribose) formation and auto-modification at Arg-185 — and linked auto-modification to cell-death resistance, while showing membrane environment reshapes substrate choice.

    Evidence Purified recombinant ART2 with HPLC, MS/MS, PI-PLC and detergent solubilization assays

    PMID:16453289 PMID:16931513

    Open questions at the time
    • Findings on ART2/ART2.1 orthologs, not human ART1
    • Physiological relevance of extracellular poly(ADP-ribose) unresolved
  7. 2007 High

    Demonstrated in vivo that the NAD+/ART2/P2X7 axis controls T cell homeostasis during inflammation and that the enzyme is inducible and redox-regulated in myeloid cells, while establishing blocking antibodies as tools.

    Evidence In vivo inflammation and NAD+ injection in ART2-KO/CD38-KO mice, BMDM induction assays, and VHH single-domain antibody blockade

    PMID:17575259 PMID:17579037 PMID:17947697

    Open questions at the time
    • ART2.1 thiol-redox regulation mechanism at the structural level not resolved
    • Distinction between ART1 and ART2 roles in vivo not parsed
  8. 2008 Medium

    Explained the muscle-restricted expression of ART1 through a defined myogenic transcriptional program.

    Evidence Promoter deletion/mutagenesis, luciferase reporters in myoblasts/myotubes, and EMSA for myogenin and MEF-2

    PMID:18939989

    Open questions at the time
    • Upstream signals controlling promoter activity in vivo not defined
    • Does not connect expression to muscle substrates
  9. 2010 High

    Connected the death axis to immune regulation and cancer: NAD+-driven ART2.2-mediated P2X7 activation depletes Tregs, and this can be exploited for antitumor immunity.

    Evidence NAD+ injection in ART2-KO and P2X7-KO mice, tumor models, and ART2.2-specific single-domain antibody protection

    PMID:20975043

    Open questions at the time
    • Mechanism of preferential Treg susceptibility not fully defined
    • ART2.2 is the mouse ortholog, not human ART1
  10. 2014 Medium

    Uncovered an atypical intracellular role: human ARTC1 in the ER ADP-ribosylates the chaperone GRP78/BiP during ER stress, coupling the enzyme to translational control.

    Evidence Immunofluorescence co-localization, macro-domain pull-down, and ER stress induction with ARTC1 overexpression

    PMID:25292337

    Open questions at the time
    • GRP78 modification site not mapped
    • Functional consequence for the unfolded protein response not established
  11. 2018 High

    Defined the in vivo muscle substrate repertoire, showing ARTC1 mediates arginine ADP-ribosylation of hundreds of surface/extracellular proteins including hemopexin.

    Evidence MS-based ADP-ribosylome profiling of WT vs ARTC1-KO myotubes and tissues with site-specific identification

    PMID:30110646

    Open questions at the time
    • Functional consequence of most modified targets unknown
    • Which substrates drive muscle phenotypes not resolved
  12. 2022 High

    Established tumor-cell ART1 as a direct immune-evasion effector that kills P2X7R+ CD8 T cells, validating it as a therapeutic target.

    Evidence In vitro NICD assay, genetic ART1 KO in tumor lines, antibody blockade in syngeneic NSCLC/melanoma models, and CD8 depletion

    PMID:35294260

    Open questions at the time
    • Whether tumor ART1 acts enzymatically in cis or trans not fully resolved
    • Source of extracellular NAD+ in the tumor microenvironment not defined
  13. 2024 Medium

    Identified VAPB-Arg50 as an intracellular ARTC1 substrate linked to calcium homeostasis and established hARTC3 as a stabilizing binding partner that promotes ARTC1 activity.

    Evidence Co-IP, ADP-ribosylation site mapping, hARTC1 knockdown, and calcium homeostasis assays

    PMID:37381178

    Open questions at the time
    • Mechanism by which VAPB modification alters calcium not defined
    • hARTC3 stabilization mechanism not structurally characterized
  14. 2023 Medium

    Linked ARTC1 to suppression of necroptosis and modulation of spontaneous tumorigenesis and cardiac injury in vivo.

    Evidence Artc1-KO and Artc1/Arh1-double-KO mice, xenografts, ischemia-reperfusion and echocardiography (preprint)

    PMID:36945646

    Open questions at the time
    • Preprint, not yet peer-reviewed
    • Direct substrate coupling ARTC1 to RIP3/necroptosis not identified
    • Tissue-specific contributions not dissected

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how ARTC1's distinct compartments (cell surface, secreted, and ER-luminal) and its mono- vs poly-ADP-ribosylation activities are coordinated, and which specific arginine-modified substrates mediate its muscle, necroptosis-suppressive, and calcium-homeostatic functions.
  • No structure of human ART1 itself
  • Substrate-to-phenotype causality largely uncharted
  • Regulation distinguishing intracellular ER activity from ecto-activity unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 3 GO:0140096 catalytic activity, acting on a protein 3 GO:0140098 catalytic activity, acting on RNA 1
Localization
GO:0005886 plasma membrane 3 GO:0005576 extracellular region 2 GO:0005783 endoplasmic reticulum 1
Pathway
R-HSA-168256 Immune System 4 R-HSA-392499 Metabolism of proteins 2 R-HSA-5357801 Programmed Cell Death 2
Partners
ARTC3GRP78/HSPA5ITGA7P2X7VAPB

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 ART2 (ARTC1 family)-catalyzed ADP-ribosylation of P2X7 purinoceptor on T cell surfaces activates the receptor, causing calcium flux, pore formation, phosphatidylserine exposure, shedding of CD62L, cell shrinkage, and propidium iodide uptake, establishing NAD-induced T cell death (NICD) via the ART2-P2X7 axis. Cell-based assays (flow cytometry, propidium iodide uptake, calcium flux), pharmacological inhibition, and ART2-deficient T cells Immunity High 14563321
2002 Crystal structure of rat ART2.2 (ortholog of ART1 family) determined at 1.7 Å resolution; the active center was identified by a bound nicotinamide analogue revealing induced-fit upon substrate binding, and the NAD+ binding mode was modeled. Two disulfide bridges distant from the active center stabilize the protein. The fold places ART2.2 in a distinct subfamily of ADP-ribosyltransferases. X-ray crystallography (1.7 Å), ligand soaking with nicotinamide analogue, structural modeling of NAD+ binding Journal of molecular biology High 12270706
2002 ART2.1/ART2.2 double-knockout T cells exhibit dramatically reduced ADP-ribosylation of cell surface proteins and are completely resistant to NAD-induced apoptosis and partially resistant to NAD-mediated suppression of proliferation, demonstrating that the ART2 ectoenzymes are essential for NAD-mediated T cell regulation. Genetic knockout mice, flow cytometry-based ADP-ribosylation assay, T cell proliferation and apoptosis assays Molecular and cellular biology High 12370300
1998 Mouse Art1 encodes a GPI-anchored ecto-enzyme predominantly expressed in cardiac and skeletal muscle. Recombinant Art1 expressed as an IgG1-Fc fusion exhibits arginine-specific ADP-ribosyltransferase activity in vitro. Northern blot, RT-PCR, recombinant expression in 293T cells, enzymatic activity assay The Biochemical journal Medium 9841866
2005 ART2.2 activity and substrate specificity depend on its GPI anchor-mediated association with lipid rafts: GPI-anchored ART2.2 showed >10-fold higher activity at limiting NAD concentrations and ADP-ribosylated a restricted set of target proteins compared to transmembrane-anchored ART2.2. Disruption of lipid rafts broadened substrate specificity. Auto-ADP-ribosylation of ART2.2 itself required GPI anchoring and raft association. Lymphoma transfectants expressing GPI- vs. transmembrane-anchored ART2.2, enzymatic activity assays, cholesterol depletion (cyclodextrin), detergent solubilization Blood High 15657180
2000 ART2.2 is shed from the T cell surface in enzymatically active form upon T cell activation via metalloprotease-mediated cleavage close to its membrane anchor (similar to TNF-alpha converting enzyme/ADAM17 cleavage of CD62L). Shed ART2.2 ADP-ribosylates substrates in vitro, and shedding correlates with reduced ADP-ribosylation capacity of the T cell surface. FACS-based ADP-ribosylation assay, metalloprotease inhibitor (Immunex Compound 3), SDS-PAGE, in vitro ADP-ribosylation assay Journal of immunology Medium 11035085
2007 NAD+ released during acute inflammation in vivo induces ART2- and P2X7-dependent depletion of naive T cells in draining lymph nodes, preferentially targeting naive over recently activated/memory T cells. This effect was absent in ART2-deficient mice, demonstrating ART2's essential role in inflammation-induced T cell homeostasis in vivo. In vivo inflammation model (polyacrylamide beads), intravenous NAD+ injection, ART2-KO and CD38-KO mice, flow cytometry, antibody response assay Journal of immunology High 17579037
2010 Regulatory T cells (Treg) express high levels of ART2.2 and P2X7; ART2.2-mediated ADP-ribosylation of P2X7 by extracellular NAD+ depletes Tregs in vivo. Selective depletion of Tregs by systemic NAD+ administration promotes antitumor responses in mouse tumor models. An inhibitory ART2.2-specific single-domain antibody protects Tregs from NAD+-induced effects. Intravenous NAD+ injection in mice, flow cytometry, ART2-KO and P2X7-KO mice, single-domain antibody blocking, tumor models The Journal of experimental medicine High 20975043
2007 Single-domain antibodies (VHH) from llama immunized against ART2.2 specifically block ART2.2 enzymatic and cytotoxic activities in vivo within 15 min of intravenous injection, with blockade reversible within 24 h. The blocking was specific for ART2.2 and did not affect the related enzymes ART1 or ART2.1. Llama immunization, VHH generation, intravenous injection in mice, flow cytometry-based ADP-ribosylation and cell death assays FASEB journal Medium 17575259
2006 ART2 (rat T cell surface isoform) catalyzes formation of extracellular poly(ADP-ribose) rather than solely mono-ADP-ribosylation; auto-ADP-ribosylation at Arg-185 produces ADP-ribose polymer identified by PR-AMP detection via HPLC and MS/MS. Intestinal intraepithelial lymphocyte ART2 undergoes multimeric auto-ADP-ribosylation more efficiently than peripheral T cell ART2, correlating with greater resistance to NAD-induced cell death. Purified recombinant ART2, sequencing gel, HPLC, MS/MS mass spectrometry, site-directed identification of Arg-185 The Journal of biological chemistry High 16931513
2007 LPS, IFN-gamma, and IFN-beta selectively induce expression of ART2.1 (but not ART2.2) as a GPI-anchored cell surface ectoenzyme in bone marrow-derived macrophages. The catalytic function of induced ART2.1 requires extracellular thiol-reducing cofactors (allosteric disulfide bond regulation). Induction is blocked by inhibitors of NF-κB, PI3K, and JAK-STAT pathways but potentiated by ERK1/2 inhibition. BMDM isolation, LPS/IFN stimulation, flow cytometry, pharmacological pathway inhibitors, ADP-ribosylation activity assay with thiol reductants Journal of immunology Medium 17947697
2008 ART1 gene expression in skeletal muscle is driven by cooperative binding of myogenin to an E-box and MEF-2 to an A/T-rich element in the proximal promoter (~1.3 kb upstream of TSS). Mutation of either element nearly abolishes promoter inducibility. Gel mobility shift assays confirmed binding of myogenin and MEF-2 restricted to myotubes. Promoter deletion analysis, luciferase reporter assay in C2C12 and C3H-10T1/2 cells, site-directed mutagenesis of E-box and A/T-rich element, gel mobility shift assay (EMSA) with nuclear extracts BMC molecular biology Medium 18939989
2014 Human ARTC1 localizes to the endoplasmic reticulum (ER) — unlike other GPI-anchored ARTC family members — and ADP-ribosylates the ER luminal chaperone GRP78/BiP. ARTC1 is activated during ER stress, resulting in acute ADP-ribosylation of GRP78/BiP coinciding with translational inhibition. Immunofluorescence co-localization, macro-domain pull-down to identify ADP-ribosylated proteins, overexpression of ARTC1, ER stress induction, co-localization with GRP78/BiP Cellular and molecular life sciences Medium 25292337
2018 ARTC1 ADP-ribosylates hundreds of arginine-containing proteins on the cell surface and in the extracellular space of skeletal muscle and heart tissue, as determined by mass spectrometry comparing wild-type and ARTC1-deficient mice. Hemopexin (HPX) was validated as an ARTC1 substrate. Target proteins are associated with signal transduction, transmembrane transport, and muscle function. Mass spectrometry-based ADP-ribosylome profiling of C2C12 myotubes and tissues from WT vs. ARTC1-KO mice, site-specific identification of arginine ADP-ribosylation Cell reports High 30110646
2005 ARTC1 peptide ligands derived from mutated tumor cell proteins are presented by MHC class II and specifically recognized by tumor-infiltrating CD4+ regulatory T cell clones, which then suppress proliferation and IL-2 secretion of melanoma-reactive T cells. Tumor cells (but not tumor lysate-pulsed B cells) directly activate these Treg clones. Establishment of CD4+ Treg clones from TILs, antigen identification by expression cloning, T cell suppression assay (proliferation, IL-2 secretion), tumor cell co-culture Journal of immunology Medium 15728473
2022 ART1 expressed on tumor cell membranes mediates ADP-ribosylation and NICD of P2X7R+ CD8 T cells, reducing their infiltration into non-small cell lung cancer. Genetic or antibody-mediated ART1 inhibition in murine NSCLC and melanoma models slowed tumor growth in a CD8 T cell-dependent manner and increased P2X7R+CD8 T cell tumor infiltration. In vitro NICD assay with P2X7R+CD8 T cells, genetic ART1 KO in tumor cell lines, antibody-mediated ART1 blockade in syngeneic mouse tumor models, CD8 T cell depletion experiments, flow cytometry Science translational medicine High 35294260
2013 ART1 silencing in mouse CT26 colon carcinoma cells enhances cisplatin-induced apoptosis, correlating with reduced phospho-Akt(Thr308), reduced phospho-IκBα, reduced NF-κB p65 nuclear translocation, decreased Bcl-2 and Bcl-xL expression, and increased Bax expression. Lentiviral shRNA knockdown of ART1, flow cytometry (apoptosis), Western blot (Akt, IκBα, NF-κB, Bcl-2 family proteins) Cellular physiology and biochemistry Medium 24335275
2015 ART1 knockdown or overexpression in CT26 colon carcinoma cells modulates Akt and Erk signaling pathway activity and expression of βIII-tubulin (Tubb3), which acts downstream of both Akt and Erk to influence apoptosis. Inhibiting either Akt or Erk downregulates Tubb3 at protein and mRNA levels, placing Tubb3 as a convergent downstream effector. Lentiviral KD and OE of ART1, in vivo allograft transplant model, Western blot, pathway inhibitors (Akt and Erk inhibitors), mRNA analysis Tumour biology Low 26373733
2015 ART1 overexpression promotes starvation-induced autophagy in CT26 colon carcinoma cells via a pathway involving increased Rac1, NF-κB, PARP-1, LKB1, and p-AMPK and decreased p-P70S6K. ART1 co-immunoprecipitates with integrin α7 in these cells. Lentiviral OE and KD, electron microscopy and LC3B Western blot (autophagy), Co-IP (ART1–integrin α7), pharmacological inhibitors (Rac1, PARP-1) American journal of cancer research Low 25973293
2024 ARTC1 ADP-ribosylates VAPB at Arg50, and knockdown of hARTC1 impairs intracellular calcium homeostasis. hARTC3 interacts with hARTC1 and stabilizes it, promoting its enzymatic activity. Co-IP (hARTC3–hARTC1 interaction), ADP-ribosylation site mapping (Arg50 of VAPB), hARTC1 knockdown, calcium homeostasis assay Journal of molecular cell biology Medium 37381178
2023 In ARTC1-KO and Artc1/Arh1 double-KO mice, spontaneous tumorigenesis is decreased and multi-organ inflammation with TNF-α upregulation is increased. In xenograft models, tumorigenicity of Arh1-KO MEFs was decreased in Artc1-KO hosts, with increased CD8+ T cell and macrophage tumor infiltration and necroptosis. Artc1-KO male mice exhibit reduced myocardial contractility and enhanced susceptibility to ischemia-reperfusion injury with increased RIP3 protein levels, suggesting ARTC1 suppresses necroptosis in heart. Artc1-KO and Artc1/Arh1-double-KO mice, spontaneous tumor monitoring, xenograft model, echocardiography/MRI, ischemia-reperfusion model, Western blot (RIP3, TNF-α), flow cytometry (immune infiltrates) bioRxivpreprint Medium 36945646
2006 ART2.1 substrate specificity is altered by the protein's membrane vs. solution environment: detergent solubilization or GPI-anchor cleavage by PI-PLC changes the spectrum of histone proteins ADP-ribosylated. Soluble ART2.1 in serum ADP-ribosylates albumin and transferrin, identified by mass spectrometry. Phospholipase C treatment, detergent solubilization, in vitro ADP-ribosylation with [32P]-NAD, SDS-PAGE, mass spectrometry identification of substrates Journal of cellular biochemistry Medium 16453289

Source papers

Stage 0 corpus · 60 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2013 Effects of interleukin-1 blockade with anakinra on adverse cardiac remodeling and heart failure after acute myocardial infarction [from the Virginia Commonwealth University-Anakinra Remodeling Trial (2) (VCU-ART2) pilot study]. The American journal of cardiology 329 23453459
2003 NAD-induced T cell death: ADP-ribosylation of cell surface proteins by ART2 activates the cytolytic P2X7 purinoceptor. Immunity 288 14563321
2009 A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. The Plant cell 247 19880795
2010 Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. The Journal of experimental medicine 176 20975043
2014 Comparative safety of interleukin-1 blockade with anakinra in patients with ST-segment elevation acute myocardial infarction (from the VCU-ART and VCU-ART2 pilot studies). The American journal of cardiology 137 25482680
2007 NAD+ released during inflammation participates in T cell homeostasis by inducing ART2-mediated death of naive T cells in vivo. Journal of immunology (Baltimore, Md. : 1950) 136 17579037
2005 Recognition of a new ARTC1 peptide ligand uniquely expressed in tumor cells by antigen-specific CD4+ regulatory T cells. Journal of immunology (Baltimore, Md. : 1950) 122 15728473
2007 Single domain antibodies from llama effectively and specifically block T cell ecto-ADP-ribosyltransferase ART2.2 in vivo. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 98 17575259
2011 Identification of a cis-acting element of ART1, a C2H2-type zinc-finger transcription factor for aluminum tolerance in rice. Plant physiology 82 21502187
2018 Proteomic Characterization of the Heart and Skeletal Muscle Reveals Widespread Arginine ADP-Ribosylation by the ARTC1 Ectoenzyme. Cell reports 59 30110646
2014 ARTC1-mediated ADP-ribosylation of GRP78/BiP: a new player in endoplasmic-reticulum stress responses. Cellular and molecular life sciences : CMLS 55 25292337
2002 Structure of the ecto-ADP-ribosyl transferase ART2.2 from rat. Journal of molecular biology 53 12270706
2005 Activity and specificity of toxin-related mouse T cell ecto-ADP-ribosyltransferase ART2.2 depends on its association with lipid rafts. Blood 52 15657180
2000 Metalloprotease-mediated shedding of enzymatically active mouse ecto-ADP-ribosyltransferase ART2.2 upon T cell activation. Journal of immunology (Baltimore, Md. : 1950) 47 11035085
2002 Generation and characterization of ecto-ADP-ribosyltransferase ART2.1/ART2.2-deficient mice. Molecular and cellular biology 44 12370300
2022 Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancer. Science translational medicine 40 35294260
2018 Functional characterization of an aluminum (Al)-inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice. The New phytologist 40 29888411
2015 Transcription factor ART1 mediates starch hydrolysis and mycotoxin production in Fusarium graminearum and F. verticillioides. Molecular plant pathology 37 26456718
2013 ART1 silencing enhances apoptosis of mouse CT26 cells via the PI3K/Akt/NF-κB pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 28 24335275
2009 A recombinant heavy chain antibody approach blocks ART2 mediated deletion of an iNKT cell population that upon activation inhibits autoimmune diabetes. Journal of autoimmunity 28 19796917
1999 The RT6 (Art2) family of ADP-ribosyltransferases in rat and mouse. Molecular and cellular biochemistry 28 10331639
2019 Methionine triggers Ppz-mediated dephosphorylation of Art1 to promote cargo-specific endocytosis. The Journal of cell biology 25 30610170
2007 Lipopolysaccharide, IFN-gamma, and IFN-beta induce expression of the thiol-sensitive ART2.1 Ecto-ADP-ribosyltransferase in murine macrophages. Journal of immunology (Baltimore, Md. : 1950) 25 17947697
2014 Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis. Molecular biology of the cell 24 25473118
2006 ART2, a T cell surface mono-ADP-ribosyltransferase, generates extracellular poly(ADP-ribose). The Journal of biological chemistry 24 16931513
2023 ART1 and putrescine contribute to rice aluminum resistance via OsMYB30 in cell wall modification. Journal of integrative plant biology 21 36515424
2017 ALUMINUM RESISTANCE TRANSCRIPTION FACTOR 1 (ART1) contributes to natural variation in aluminum resistance in diverse genetic backgrounds of rice (O. sativa). Plant direct 21 31245663
2009 Basal and inducible expression of the thiol-sensitive ART2.1 ecto-ADP-ribosyltransferase in myeloid and lymphoid leukocytes. Purinergic signalling 19 19404775
1998 Molecular characterization and expression of the gene for mouse NAD+:arginine ecto-mono(ADP-ribosyl)transferase, Art1. The Biochemical journal 19 9841866
1980 Biochemical identification of rat ART-1 and Ly-1 alloantigens. Immunogenetics 18 22457937
2015 Tubb3 regulation by the Erk and Akt signaling pathways: a mechanism involved in the effect of arginine ADP-ribosyltransferase 1 (Art1) on apoptosis of colon carcinoma CT26 cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 17 26373733
2019 Yeast α-arrestin Art2 is the key regulator of ubiquitylation-dependent endocytosis of plasma membrane vitamin B1 transporters. PLoS biology 16 31658248
2018 Blocking the ART2.2/P2X7-system is essential to avoid a detrimental bias in functional CD4 T cell studies. European journal of immunology 16 29508376
2017 Effect of ART1 on the proliferation and migration of mouse colon carcinoma CT26 cells in vivo. Molecular medicine reports 15 28138708
2015 Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets. Immunobiology 15 26307000
2001 A hematopoietic-specific transmembrane protein, Art-1, is possibly regulated by AML1. Biochemical and biophysical research communications 15 11396961
2015 ART1 promotes starvation-induced autophagy: a possible protective role in the development of colon carcinoma. American journal of cancer research 14 25973293
2012 NAD induces astrocyte calcium flux and cell death by ART2 and P2X7 pathway. The American journal of pathology 14 22781627
2008 Identification of two regulatory binding sites which confer myotube specific expression of the mono-ADP-ribosyltransferase ART1 gene. BMC molecular biology 12 18939989
2003 Triggering of T-cell apoptosis by toxin-related ecto-ADP-ribosyltransferase ART2. Annals of the New York Academy of Sciences 9 15033737
2024 ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells. BMC cancer 8 38504172
2002 Expression, purification, crystallization and preliminary X-ray analysis of rat ecto-ADP-ribosyltransferase 2 (ART2.2). Acta crystallographica. Section D, Biological crystallography 8 12077446
2001 Levels of Art2+ cells but not soluble Art2 protein correlate with expression of autoimmune diabetes in the BB rat. Autoimmunity 8 11683379
2023 Overexpression of an ART1-Interacting Gene OsNAC016 Improves Al Tolerance in Rice. International journal of molecular sciences 7 38069359
2018 Investigation of Ldb19/Art1 localization and function at the late Golgi. PloS one 7 30403748
2008 Articulospora sp. produces Art1, an inhibitor of bacterial histidine kinase. Bioscience, biotechnology, and biochemistry 7 18838822
2023 Art2 mediates selective endocytosis of methionine transporters during adaptation to sphingolipid depletion. Journal of cell science 6 37337792
2001 Changing patterns of cell surface mono (ADP-ribosyl) transferase antigen ART2.2 on resting versus cytopathically-activated T cells in NOD/Lt mice. Diabetologia 6 11508269
2024 Pan-Cancer Analysis of ART1 and its Potential Value in Gastric Cancer. Journal of Cancer 5 38911388
2011 Transgenic overexpression of toxin-related ecto-ADP-ribosyltransferase ART2.2 sensitizes T cells but not B cells to NAD-induced cell death. Molecular immunology 4 21641039
2025 Computational Exploration of Bacterial Compounds Targeting Arginine-Specific Mono-Adp-Ribosyl-Transferase 1 (Art1): A Pathway to Novel Therapeutic Anticancer Strategies. Current issues in molecular biology 3 40864788
2024 ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis. Journal of molecular cell biology 3 37381178
2015 Evidence of a role for S. cerevisiae α-arrestin Art1 (Ldb19) in mating projection and zygote formations. Cell biology international 3 26314564
2001 Fetal thymi from diabetes-prone but not diabetes-resistant BB/Wor rats fail to generate mature ART2+ T-cells in organ culture. Cellular and molecular biology (Noisy-le-Grand, France) 2 11292261
2025 Comparative Transcriptome Reveals ART1-Dependent Regulatory Pathways for Fe Toxicity Response in Rice Roots. Physiologia plantarum 1 40653961
2025 Effect of ART1 on the efficacy of oxaliplatin in colorectal cancer under high-cholesterol conditions. Histology and histopathology 0 41346334
2025 Role of Art1 in Sperm Damage Induced by Type 2 Diabetes Mellitus. Molecular reproduction and development 0 41361964
2023 Mono-ADP-ribosyltransferase 1 ( Artc1 )-deficiency decreases tumorigenesis, increases inflammation, decreases cardiac contractility, and reduces survival. bioRxiv : the preprint server for biology 0 36945646
2006 Substrate specificity of soluble and membrane-associated ADP-ribosyltransferase ART2.1. Journal of cellular biochemistry 0 16453289
2005 Characterization of multiple alleles of the T-cell differentiation marker ART2 (RT6) in inbred and wild rats. Immunogenetics 0 16195877

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