Affinage

ARHGEF7

Rho guanine nucleotide exchange factor 7 · UniProt Q14155

Round 2 corrected
Length
803 aa
Mass
90.0 kDa
Annotated
2026-04-28
48 papers in source corpus 18 papers cited in narrative 18 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARHGEF7 (β-PIX/Cool-1) is a multidomain Rho guanine nucleotide exchange factor that activates Rac1, Cdc42, and TC10 through its DH-PH domains and scaffolds PAK kinases via its SH3 domain at focal adhesions and lamellipodia, thereby coordinating actin cytoskeletal remodeling, cell migration, and focal adhesion turnover (PMID:9659915, PMID:21423176, PMID:29891904). Beyond its GEF activity, ARHGEF7 functions as a GEF-independent scaffold that bridges LATS kinase to YAP/TAZ in the Hippo pathway and maintains podocyte survival through Cdc42–YAP signaling, with podocyte-specific knockout causing progressive glomerulosclerosis (PMID:25425573, PMID:32188698). In neurons, ARHGEF7 is essential for axon formation, neurite outgrowth, cerebellar granule cell development, and cortical dendritic maintenance, operating through TC10 activation and Wnt pathway engagement (PMID:29891904, PMID:41585483, PMID:40598577). ARHGEF7 also participates in LRRK2 GTPase regulation and Golgi maintenance, is targeted for proteasomal degradation by KLHL2-mediated ubiquitination, and regulates actomyosin-driven von Willebrand factor exocytosis from endothelial Weibel-Palade bodies (PMID:21048939, PMID:33163274, PMID:42024489).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1998 High

    Identification of ARHGEF7 as a GEF that directly binds PAK kinases via its SH3 domain established the molecular basis for coupling Rho GTPase activation to PAK-dependent actin remodeling at focal complexes.

    Evidence Protein purification, binding assays (Kd ~24 nM), co-localization in focal complexes, membrane ruffling assays; independently confirmed as Cool-1 with isoform-specific PAK regulation

    PMID:9659915 PMID:9726964

    Open questions at the time
    • Crystal structure of the SH3–PAK interaction was not resolved
    • Whether GEF activity and PAK scaffolding are independently regulated was unclear
    • Relative contributions of Rac1 vs Cdc42 activation were not distinguished
  2. 1999 High

    Discovery of the paxillin–p95PKL–PIX–PAK quaternary complex at focal adhesions explained how ARHGEF7 is physically recruited to integrin adhesion sites and linked GTPase signaling to focal adhesion dynamics.

    Evidence Reciprocal co-immunoprecipitation, GFP localization, dominant-negative overexpression, and microinjection with migration assays

    PMID:10330411

    Open questions at the time
    • How the complex is dynamically assembled and disassembled during migration was unknown
    • Whether additional scaffolds participate was not addressed
  3. 2010 High

    Two parallel advances broadened ARHGEF7's functional scope: it was shown to activate Rac1 downstream of podocalyxin–EBP50–ezrin to promote cell migration, and independently demonstrated to serve as a GEF for LRRK2 GTPase activity while being phosphorylated by LRRK2 kinase, linking ARHGEF7 to Parkinson's disease-associated signaling.

    Evidence Rac1 pulldown assays with knockdown/rescue for podocalyxin axis; in vitro GTPase and kinase assays with LRRK2 wild-type and R1441C mutant

    PMID:20395446 PMID:21048939

    Open questions at the time
    • LRRK2 phosphorylation sites on ARHGEF7 were mapped but functional consequences of individual sites were not dissected
    • Whether podocalyxin and LRRK2 pathways converge on the same ARHGEF7 pool was not tested
  4. 2011 High

    Quantitative proteomic and live-cell imaging studies demonstrated that ARHGEF7 negatively regulates focal adhesion maturation and promotes lamellipodial protrusion, establishing it as a key node where Rac-driven protrusion opposes myosin II-driven contractility.

    Evidence Proteomic analysis of isolated focal adhesions ± myosin II inhibition, live-cell imaging, knockdown phenotyping

    PMID:21423176

    Open questions at the time
    • Direct mechanism by which myosin II suppresses ARHGEF7 focal adhesion enrichment was not resolved
    • Whether GEF activity or scaffolding drives focal adhesion turnover was not distinguished
  5. 2012 High

    Epistatic rescue experiments during cytokinesis showed that CYK4 GAP spatially restricts ARHGEF7-dependent Rac1–adhesion signaling at the cell equator, revealing ARHGEF7 as a target of negative regulation essential for proper cytokinetic ring formation.

    Evidence siRNA depletion of ARHGEF7 rescued cytokinesis defects and elevated vinculin phenotypes of CYK4 GAP mutant

    PMID:22945935

    Open questions at the time
    • Whether ARHGEF7 is directly a CYK4 substrate or regulated indirectly via Rac1 was not resolved
    • Role in non-adherent cell cytokinesis was not tested
  6. 2014 High

    Two studies placed ARHGEF7 in new signaling contexts: CK1α-mediated LRRK2 phosphorylation modulates LRRK2–ARHGEF7 binding to control Golgi-derived vesicle dynamics, and ARHGEF7 was identified as a GEF-independent scaffold that bridges LATS to YAP/TAZ to positively regulate Hippo signaling independently of its catalytic activity.

    Evidence Kinome screen with co-IP and subcellular fractionation for CK1α–LRRK2 axis; co-IP, GEF-dead mutant analysis, knockdown/overexpression, nuclear-cytoplasmic fractionation for Hippo pathway

    PMID:25425573 PMID:25500533

    Open questions at the time
    • Structural basis for GEF-independent LATS–YAP scaffolding was not determined
    • Whether Hippo regulation and Golgi maintenance share the same ARHGEF7 pool was untested
    • Contribution of Hippo scaffolding vs GEF activity to tumor-suppressive function in breast cancer was not separated in vivo
  7. 2018 High

    Demonstration that ARHGEF7 is essential for axon formation via TC10 (not Cdc42) expanded GTPase substrate specificity beyond Rac1/Cdc42, as constitutively active TC10 but not Cdc42 rescued axon loss in ARHGEF7-depleted cortical neurons.

    Evidence In utero electroporation knockdown, cultured neuron morphology, rescue with constitutively active GTPase mutants, co-immunoprecipitation

    PMID:29891904

    Open questions at the time
    • Direct in vitro GEF assay for TC10 was not performed
    • Whether TC10 activation is relevant outside axon formation was not tested
  8. 2019 High

    Identification of a STIL–ARHGEF7–PAK1 ternary complex at lamellipodia revealed a positive-feedback loop in which STIL promotes ARHGEF7-mediated Rac1 activation and PAK1 substrate phosphorylation for polarized cancer cell migration.

    Evidence Co-immunoprecipitation, dual siRNA epistasis, PAK1 substrate phosphorylation and Rac1 activity assays

    PMID:31754215

    Open questions at the time
    • Whether STIL directly enhances ARHGEF7 catalytic activity or only facilitates complex assembly was not distinguished
    • In vivo relevance for metastasis was not tested
  9. 2020 High

    In vivo conditional knockout established ARHGEF7 as the predominant Cdc42-activating GEF in podocytes, with its loss causing progressive glomerulosclerosis through reduced YAP pro-survival signaling, and separately KLHL2 was identified as the E3 ligase directing ARHGEF7 to proteasomal degradation.

    Evidence BioID proximity ligation and podocyte-specific KO mice with Cdc42/YAP activity assays; ubiquitination assays and domain mapping for KLHL2

    PMID:32188698 PMID:33163274

    Open questions at the time
    • Whether KLHL2-mediated degradation is regulated by upstream signals was unknown
    • Whether YAP loss in podocytes reflects GEF-dependent or GEF-independent ARHGEF7 function was not dissected
  10. 2025 High

    Conditional knockout and overexpression studies demonstrated ARHGEF7 is required for cerebellar granule cell proliferation, migration, and differentiation, and separately that cortical ARHGEF7 maintains dendrites and synapses and protects against β-amyloid toxicity via Wnt signaling activation.

    Evidence Conditional KO mice with quantified cerebellar developmental phenotypes; viral overexpression/knockdown in mPFC with behavioral tests and Wnt reporter assays

    PMID:40598577 PMID:41585483

    Open questions at the time
    • The GTPase substrate mediating cerebellar phenotypes was not identified
    • Whether Wnt pathway engagement is direct or downstream of Rac1/Cdc42 was not resolved
    • Cortical Wnt findings are from a single study
  11. 2026 High

    Live-cell imaging and domain truncation analysis revealed that ARHGEF7 regulates actomyosin ring kinetics during Weibel-Palade body exocytosis in endothelial cells, coordinating septin ring formation and cofilin-mediated actin remodeling for efficient VWF secretion.

    Evidence siRNA knockdown, GFP-VWF live-cell imaging with kinetic quantification, truncated mutant rescue, septin and cofilin localization

    PMID:42024489

    Open questions at the time
    • Whether ARHGEF7 acts on Rac1 or Cdc42 during WPB exocytosis was not specified
    • In vivo hemostatic consequences were not assessed

Open questions

Synthesis pass · forward-looking unresolved questions
  • A unifying structural and regulatory model explaining how ARHGEF7 switches between GEF-dependent (Rac1/Cdc42/TC10 activation) and GEF-independent (Hippo scaffolding) functions, and how KLHL2-mediated degradation is coupled to upstream signals, remains to be established.
  • No high-resolution structure of full-length ARHGEF7 or its complexes exists
  • How isoform-specific differences (p50 vs p85 vs β-PIX-d) determine pathway selectivity is unresolved
  • In vivo demonstration of ARHGEF7 as a bona fide TC10 GEF by direct biochemical assay is lacking

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003924 GTPase activity 6 GO:0060090 molecular adaptor activity 3 GO:0008092 cytoskeletal protein binding 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005856 cytoskeleton 5 GO:0005886 plasma membrane 3 GO:0005794 Golgi apparatus 1 GO:0005829 cytosol 1 GO:0031410 cytoplasmic vesicle 1
Pathway
R-HSA-162582 Signal Transduction 6 R-HSA-112316 Neuronal System 3 R-HSA-1266738 Developmental Biology 3 R-HSA-1500931 Cell-Cell communication 3 R-HSA-109582 Hemostasis 1 R-HSA-392499 Metabolism of proteins 1
Partners
GIT1KLHL2LATS1LRRK2PAK1PAK3STILYAP1
Complex memberships
Paxillin–GIT–PIX–PAK complexPodocalyxin–EBP50–ezrin–βPIX complexSTIL–βPIX–PAK1 complex

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 ARHGEF7 (PIX/Cool-1) was identified as a guanine nucleotide exchange factor that binds tightly to PAK kinases through its N-terminal SH3 domain interacting with a conserved proline-rich PAK sequence (Kd ~24 nM). PIX is enriched in Cdc42- and Rac1-driven focal complexes, is required for PAK recruitment to these sites, and can induce membrane ruffling with associated Rac1 activation, suggesting a role in Cdc42-to-Rac1 signaling. Protein purification, cloning, binding assays, co-localization in focal complexes, membrane ruffling assays Molecular cell High 9659915
1998 ARHGEF7 (Cool-1) was identified as a PAK3-binding protein with SH3, DH, and PH domains. The p50 isoform binding to PAK3 represses PAK3 activation by upstream activators such as the Dbl oncoprotein, revealing a novel regulatory mechanism for PAK signaling; the p85 isoform did not show this repressive activity. Library cloning, co-immunoprecipitation, kinase activity assays, isoform comparison The Journal of biological chemistry High 9726964
1999 ARHGEF7 (PIX) is recruited to focal adhesions as part of a complex with PAK, Nck, and the scaffolding protein p95PKL (paxillin-kinase linker), which bridges PIX to the paxillin LD4 motif. This paxillin–p95PKL–PIX–PAK complex links paxillin to p21 GTPase-regulated actin cytoskeletal reorganization at focal adhesions. Co-immunoprecipitation, pulldown, GFP localization, dominant-negative overexpression, microinjection with cell migration assay The Journal of cell biology High 10330411
2010 ARHGEF7 (β-PIX) forms a complex with podocalyxin, EBP50 (ERM-binding phosphoprotein 50), and ezrin, which activates Rac1 to promote cell migration. Podocalyxin knockdown reduced Rac1 activity and migration; re-expression restored them. Podocalyxin overexpression in HEK cells upregulated Rac1 activity in an ARHGEF7-dependent manner. siRNA knockdown, co-immunoprecipitation, Rac1 activity assay (GST-PAK pulldown), overexpression rescue The American journal of pathology High 20395446
2010 ARHGEF7 (β-PIX) interacts with LRRK2 in vitro and in vivo, acts as a guanine nucleotide exchange factor for LRRK2 (stimulating its GTPase activity), and is phosphorylated by LRRK2 at previously unknown sites. The Parkinson's disease-associated R1441C LRRK2 mutant shows reduced GTP hydrolysis and reduced binding to ARHGEF7. Co-immunoprecipitation, in vitro GTPase activity assay, in vitro kinase assay, phosphorylation site mapping, mutant binding studies PloS one High 21048939
2011 ARHGEF7 (β-PIX) localizes to focal adhesions and negatively regulates focal adhesion maturation. It is enriched in focal adhesions upon myosin II inhibition (conditions favoring Rac-mediated lamellipodia), and promotes lamellipodial protrusion and focal adhesion turnover to drive cell migration. Its abundance in focal adhesions is inversely regulated by myosin II-driven contractility. Proteomic analysis of isolated focal adhesions, quantitative MS comparison ±myosin II inhibition, live-cell imaging, knockdown with defined phenotypic readouts Nature cell biology High 21423176
2012 ARHGEF7 is a Rac1-specific effector pathway component negatively regulated by CYK4 GAP activity during cytokinesis. The MKlp1-CYK4 centralspindlin complex acts as a GAP for Rac1 (not RhoA) at the cell equator in anaphase. Depletion of ARHGEF7 rescued cytokinesis defects and elevated vinculin/adhesion phenotypes caused by expression of a CYK4 GAP mutant, demonstrating that CYK4 inhibits ARHGEF7-dependent cell adhesion pathways to spatially segregate adhesion from contractile ring formation. siRNA depletion, rescue genetics (epistasis), immunofluorescence for vinculin, GAP activity assays The Journal of cell biology High 22945935
2013 In LRRK2-knockdown primary hippocampal neurons, ARHGEF7 is mislocalized and dysregulated at growth cones, leading to enhanced neurite branching and increased growth cones per neuron. ARHGEF7 and Tropomyosin 4 (TPM4) co-regulate actin polymerization at growth cones downstream of LRRK2, with LRRK2 knockdown and ARHGEF7 knockdown showing convergent effects on neurite outgrowth. LRRK2 knockdown in primary neurons, transcriptome profiling, immunofluorescence localization, neurite morphology quantification Biochimica et biophysica acta Medium 24075941
2014 CK1α phosphorylates LRRK2, and this phosphorylation modulates LRRK2 recruitment to TGN46-positive Golgi-derived vesicles through differential interaction with ARHGEF7. Constitutive LRRK2 phosphorylation by CK1α regulates trans-Golgi clustering via altered ARHGEF7 binding, placing ARHGEF7 in a CK1α→LRRK2→ARHGEF7 signaling axis for Golgi maintenance. siRNA kinome screen, co-immunoprecipitation, subcellular fractionation/localization, phosphorylation assays Nature communications High 25500533
2014 ARHGEF7 (βPix) binds both LATS kinase and YAP/TAZ and acts as a positive regulator of the Hippo pathway by scaffolding LATS-mediated phosphorylation of YAP/TAZ in a GEF-independent manner. βPix localizes to the cytoplasm and is required downstream of both cell density sensing and actin cytoskeletal rearrangements. Loss of βPix reduces YAP/TAZ phosphorylation and promotes nuclear YAP/TAZ localization and target gene expression; conversely, increased βPIX in breast cancer cell lines restores cytoplasmic YAP/TAZ and inhibits migration and proliferation. Co-immunoprecipitation, phosphorylation assays, knockdown/overexpression, nuclear/cytoplasmic fractionation, reporter assays, GEF-dead mutant analysis The EMBO journal High 25425573
2018 Arhgef7 is essential for axon formation during cortical development. Loss of Arhgef7 causes extensive loss of axons in cultured neurons and in the developing cortex. Although Arhgef7 is a known GEF for Cdc42, active Cdc42 could not rescue Arhgef7 knockdown. Instead, Arhgef7 was found to interact with and act upstream of the GTPase TC10 (closely related to Cdc42), and expression of active TC10 restored axon extension in Arhgef7-deficient neurons. In utero electroporation knockdown, cultured neuron morphology, rescue experiments with active GTPases, co-immunoprecipitation Scientific reports High 29891904
2019 ARHGEF7 forms a ternary complex with STIL and PAK1 that accumulates at lamellipodia of migrating cancer cells. STIL knockdown impairs accumulation of the ARHGEF7-PAK1 complex at membrane ruffles, reduces PAK1 substrate phosphorylation, and attenuates cortical actin remodeling. ARHGEF7 knockdown similarly reduces STIL and PAK1 accumulation at membrane ruffles and diminishes Rac1 activity at the leading edge, implicating STIL in ARHGEF7-mediated positive-feedback Rac1 activation for polarized cell migration. Co-immunoprecipitation, siRNA knockdown, immunofluorescence localization, PAK1 substrate phosphorylation assay, Rac1 activity assay Oncogene High 31754215
2020 ARHGEF7 (β-PIX) is a predominant GEF that activates Cdc42 in human podocytes, identified by proximity-ligation BioID. Podocyte-specific β-PIX knockout mice develop progressive proteinuria and glomerulosclerosis with podocyte loss. Loss of β-PIX reduces Cdc42 activity in podocytes and promotes podocyte apoptosis via reduced activity of the pro-survival transcriptional regulator YAP (Yes-associated protein). BioID proximity ligation, conditional knockout mice, Cdc42 activity assay, shRNA knockdown, apoptosis assays, YAP activity measurement Journal of the American Society of Nephrology : JASN High 32188698
2020 ARHGEF7 is targeted for ubiquitin-proteasome degradation by the E3 ubiquitin ligase KLHL2. The Kelch domain of KLHL2 is necessary for binding ARHGEF7. KLHL2 overexpression promotes ARHGEF7 ubiquitination and degradation; KLHL2 and ARHGEF7 expression levels are inversely correlated in clear cell renal cell carcinoma. Co-immunoprecipitation, ubiquitination assays, proteasome inhibitor treatment, domain deletion mapping, knockdown/overexpression functional assays American journal of cancer research High 33163274
2024 The β-PIX-d isoform of ARHGEF7 activates Rac1 and induces neuritogenesis in primary cortical neurons. Overexpression of β-PIX-d in the embryonic neocortex induced neuronal clustering and enhanced neurite outgrowth with misplacement of layer V-VI neurons. In primary neocortical cultures, β-PIX-d overexpression increased Rac1 activity as detected by GST-PAK pulldown assays. In utero electroporation, histological analysis, layer-specific marker immunofluorescence, Rac1 activity pulldown assay Experimental neurobiology Medium 39568178
2025 Arhgef7 is expressed in granule cell precursors (GCPs) of the developing cerebellum and is essential for GCP proliferation, migration, and differentiation. Conditional knockout of Arhgef7 in GCPs causes severe cerebellar hypoplasia, foliation defects (especially lobules VI/VII), reduced postnatal GCP proliferation, disorganized cell layers, delayed differentiation, and impaired tangential and radial migration. Conditional knockout mice (cKO), histology, immunofluorescence, cell proliferation assays, migration quantification iScience High 41585483
2025 Arhgef7 in medial prefrontal cortex neurons is essential for dendritic and synaptic maintenance and protects cortical neurons from β-amyloid toxicity by activating the Wnt signaling pathway. Overexpression of Arhgef7 in mPFC neurons enhanced dendritic and synaptic growth and alleviated spatial cognitive impairments; knockdown caused opposite effects. Wnt pathway activation was identified as the downstream mechanism. Transcriptome sequencing, in vivo knockdown and overexpression (viral vectors), behavioral tests, neuropathological analysis, Wnt pathway reporter assays Alzheimer's research & therapy Medium 40598577
2026 ARHGEF7 (β-PIX) regulates actomyosin-mediated exocytosis of von Willebrand factor (VWF) from Weibel-Palade bodies in endothelial cells. β-PIX depletion reduces WPB fusion events, prolongs VWF secretion post-fusion, and delays exocytic actomyosin ring kinetics. Domain analysis showed the PAK-interacting and GEF domains mediate cytoskeletal remodeling, while full-length β-PIX is required for VWF secretion. β-PIX regulates both septin ring formation and cofilin-mediated actin remodeling during actomyosin ring function. siRNA knockdown, live-cell imaging (GFP-VWF), truncated mutant rescue, septin and cofilin localization analysis Molecular biology of the cell High 42024489

Source papers

Stage 0 corpus · 48 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2006 Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 2861 17081983
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1998 PAK kinases are directly coupled to the PIX family of nucleotide exchange factors. Molecular cell 647 9659915
2005 High-throughput mapping of a dynamic signaling network in mammalian cells. Science (New York, N.Y.) 553 15761153
2011 Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature cell biology 490 21423176
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1999 Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling. The Journal of cell biology 402 10330411
1996 Normalization and subtraction: two approaches to facilitate gene discovery. Genome research 401 8889548
2010 Thirty new loci for age at menarche identified by a meta-analysis of genome-wide association studies. Nature genetics 392 21102462
2004 Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. Current biology : CB 386 15324660
2004 14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking. The Biochemical journal 372 14744259
2008 Deregulation of scribble promotes mammary tumorigenesis and reveals a role for cell polarity in carcinoma. Cell 343 19041750
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
1998 A novel regulator of p21-activated kinases. The Journal of biological chemistry 270 9726964
2022 Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Cell 256 35063084
2016 A High-Density Map for Navigating the Human Polycomb Complexome. Cell reports 216 27705803
2014 Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes. Journal of proteomics 215 25281560
2012 CYK4 inhibits Rac1-dependent PAK1 and ARHGEF7 effector pathways during cytokinesis. The Journal of cell biology 90 22945935
2014 Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. Nature communications 86 25500533
2010 Podocalyxin EBP50 ezrin molecular complex enhances the metastatic potential of renal cell carcinoma through recruiting Rac1 guanine nucleotide exchange factor ARHGEF7. The American journal of pathology 80 20395446
2010 ARHGEF7 (Beta-PIX) acts as guanine nucleotide exchange factor for leucine-rich repeat kinase 2. PloS one 54 21048939
2013 LRRK2 guides the actin cytoskeleton at growth cones together with ARHGEF7 and Tropomyosin 4. Biochimica et biophysica acta 51 24075941
2014 Arhgef7 promotes activation of the Hippo pathway core kinase Lats. The EMBO journal 33 25425573
2018 The guanine nucleotide exchange factor Arhgef7/βPix promotes axon formation upstream of TC10. Scientific reports 30 29891904
2018 ARHGEF7 promotes metastasis of colorectal adenocarcinoma by regulating the motility of cancer cells. International journal of oncology 29 30132516
2020 ARHGEF7 (β-PIX) Is Required for the Maintenance of Podocyte Architecture and Glomerular Function. Journal of the American Society of Nephrology : JASN 17 32188698
2019 Indispensable role of STIL in the regulation of cancer cell motility through the lamellipodial accumulation of ARHGEF7-PAK1 complex. Oncogene 17 31754215
2021 Three PilZ Domain Proteins, PlpA, PixA, and PixB, Have Distinct Functions in Regulation of Motility and Development in Myxococcus xanthus. Journal of bacteriology 8 33875546
2020 Ubiquitin ligase KLHL2 promotes the degradation and ubiquitination of ARHGEF7 protein to suppress renal cell carcinoma progression. American journal of cancer research 7 33163274
2018 Generalized epilepsy and mild intellectual disability associated with 13q34 deletion: A potential role for SOX1 and ARHGEF7. Seizure 6 29734022
2018 Characterization of the pixB gene in Xenorhabdus nematophila and discovery of a new gene family. Microbiology (Reading, England) 2 29498622
2025 Arhgef7 as a key target for enriched environment rescuing spatial cognitive deficits and anxiety-like behaviors in a mouse model of Alzheimer's disease following early social isolation. Alzheimer's research & therapy 1 40598577
2024 β-PIX-d, a Member of the ARHGEF7 Guanine Nucleotide Exchange Factor Family, Activates Rac1 and Induces Neuritogenesis in Primary Cortical Neurons. Experimental neurobiology 1 39568178
2026 Endothelial β-PIX (ARHGEF7) supports actomyosin mediated expulsion of VWF through dynamic reorganization of the cytoskeleton. Molecular biology of the cell 0 42024489
2025 Arhgef7 is essential for granule cell precursor proliferation and migration during cerebellum development. iScience 0 41585483