{"gene":"ZNF536","run_date":"2026-06-11T09:02:07","timeline":{"discoveries":[{"year":2009,"finding":"ZNF536 is a zinc finger protein that negatively regulates neuronal differentiation: overexpression inhibits retinoic acid (RA)-induced neuronal differentiation in P19 cells, while depletion or mutation enhances differentiation. It acts by inhibiting RA response element-mediated transcriptional activity, and overexpression of RA receptor alpha rescues the inhibitory effect of ZNF536.","method":"Overexpression and knockdown/mutation in P19 cells with RA-induced differentiation; RARE-reporter assays; rescue by RARα overexpression","journal":"Molecular and cellular biology","confidence":"High","confidence_rationale":"Tier 2 / Strong — multiple orthogonal methods (gain-of-function, loss-of-function, reporter assay, rescue experiment) in a single focused mechanistic study","pmids":["19398580"],"is_preprint":false},{"year":2009,"finding":"ZNF536 is predominantly expressed in the brain, specifically in the developing central nervous system (cerebral cortex, hippocampus, hypothalamic area, dorsal root ganglia), and its expression increases at early stages of RA-induced neuronal differentiation in P19 cells.","method":"Expression profiling during P19 differentiation; regional brain localization studies","journal":"Molecular and cellular biology","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — direct localization data from a single focused study, replicated conceptually across multiple papers","pmids":["19398580"],"is_preprint":false},{"year":2003,"finding":"The KIAA0390 protein (ZNF536 alias) binds specifically to the DNA core sequence CCCCC(A), the same consensus recognized by ZNF219, as demonstrated by random oligonucleotide selection assay and electromobility gel shift assay.","method":"Random oligonucleotide selection assay (SELEX) and electromobility gel shift assay (EMSA) with expressed KIAA0390 protein","journal":"DNA research","confidence":"Medium","confidence_rationale":"Tier 1 / Weak — in vitro biochemical binding assay, single lab, single study","pmids":["14621294"],"is_preprint":false},{"year":2019,"finding":"znf536 (zebrafish ortholog) is required for the development of forebrain neurons implicated in social behavior and stress, as revealed by single-cell RNA sequencing and mutant phenotyping in zebrafish.","method":"Zebrafish mutagenesis screen; whole-brain activity mapping; single-cell RNA sequencing; behavioral profiling","journal":"Cell","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — loss-of-function in zebrafish with defined anatomical and cellular phenotype, orthogonal methods (scRNA-seq + behavioral + brain imaging)","pmids":["30929901"],"is_preprint":false},{"year":2024,"finding":"znf536 knockout zebrafish display decreased cerebellar volume with reduced populations of specific neuronal cells, especially in the valvular cerebelli (Va), and disorganization of a myelin structure within the Va, establishing ZNF536 as required for cerebellar development.","method":"CRISPR/Cas9 knockout zebrafish; brain volumetric analysis; transgenic myelin reporter line Tg[mbp:mgfp]; behavioral assays (anxiety, social interaction)","journal":"Translational psychiatry","confidence":"High","confidence_rationale":"Tier 2 / Strong — clean KO with multiple orthogonal readouts (anatomy, cell populations, myelin structure, behavior) in a dedicated mechanistic study","pmids":["38331943"],"is_preprint":false},{"year":2025,"finding":"CRISPR/Cas9 knockout of ZNF536 in SH-SY5Y cells impairs activation of retinoic acid receptor (RAR) target genes during ATRA-induced differentiation, reduces neurite outgrowth, causes failure of neuronal maturation, and dysregulates E2F4-mediated cell cycle pathways. An intronic deletion within ZNF536 also altered expression of multiple schizophrenia-associated genes, indicating cis-regulatory function.","method":"CRISPR/Cas9 dual-allelic knockout (103 kb intronic deletion + zinc finger domain disruption); transcriptome profiling (RNA-seq); GSEA; neurite outgrowth measurement","journal":"Frontiers in molecular neuroscience","confidence":"High","confidence_rationale":"Tier 2 / Moderate — clean KO with multiple orthogonal methods (transcriptomics, cell morphology, pathway analysis) in a single dedicated mechanistic study","pmids":["41163642"],"is_preprint":false}],"current_model":"ZNF536 is a brain-enriched C2H2 zinc finger transcriptional repressor that binds the DNA consensus sequence CCCCC(A) and negatively regulates neuronal differentiation by suppressing retinoic acid response element-mediated transcription; loss of ZNF536 in both zebrafish and human neuronal cell models impairs RAR target gene activation, reduces neurite outgrowth, disrupts cerebellar development (particularly in the valvular cerebelli), and affects forebrain neurons involved in social behavior and stress responses."},"narrative":{"mechanistic_narrative":"ZNF536 is a brain-enriched C2H2 zinc finger transcription factor that acts as a negative regulator of neuronal differentiation [PMID:19398580]. It binds the DNA core consensus CCCCC(A) [PMID:14621294] and suppresses retinoic acid response element (RARE)-mediated transcription, with overexpression inhibiting RA-induced neuronal differentiation in P19 cells while depletion or mutation enhances it; restoring RARα rescues this repressive effect, placing ZNF536 upstream of retinoic acid receptor signaling [PMID:19398580]. Consistent with this role, ZNF536 loss in human SH-SY5Y neurons impairs activation of RAR target genes during ATRA-induced differentiation, reduces neurite outgrowth, blocks neuronal maturation, and dysregulates E2F4-linked cell cycle pathways [PMID:41163642]. In zebrafish, loss of the znf536 ortholog disrupts development of forebrain neurons implicated in social behavior and stress [PMID:30929901] and reduces cerebellar volume with loss of specific neuronal populations and myelin disorganization in the valvular cerebelli [PMID:38331943], establishing a conserved requirement in brain development. The gene also harbors cis-regulatory sequence influencing expression of schizophrenia-associated genes [PMID:41163642].","teleology":[{"year":2003,"claim":"Established the DNA sequence specificity of the ZNF536 protein, defining how it could engage target promoters.","evidence":"SELEX and EMSA with expressed KIAA0390 (ZNF536) protein","pmids":["14621294"],"confidence":"Medium","gaps":["In vitro binding only; no endogenous genomic targets identified","Functional consequence of CCCCC(A) binding not tested","Overlap with ZNF219 specificity leaves target discrimination unresolved"]},{"year":2009,"claim":"Defined ZNF536 as a repressor of RA-driven neuronal differentiation, linking its zinc finger activity to a specific developmental signaling pathway.","evidence":"Overexpression, knockdown/mutation, RARE-reporter assays, and RARα rescue in RA-differentiated P19 cells; brain expression profiling","pmids":["19398580"],"confidence":"High","gaps":["Direct target genes repressed by ZNF536 not mapped","Mechanism of RARE suppression (co-repressor recruitment vs. direct competition) unknown","Link between CCCCC(A) binding and RARE regulation not established"]},{"year":2019,"claim":"Extended ZNF536 function to in vivo brain development, showing it is required for forebrain neurons governing social behavior and stress.","evidence":"Zebrafish mutagenesis, whole-brain activity mapping, single-cell RNA-seq, and behavioral profiling","pmids":["30929901"],"confidence":"Medium","gaps":["Molecular targets in forebrain neurons not identified","Connection to RA signaling in vivo not tested","Cell-autonomy of the requirement unresolved"]},{"year":2024,"claim":"Pinpointed a discrete anatomical requirement for ZNF536 in cerebellar development and myelin organization.","evidence":"CRISPR/Cas9 knockout zebrafish with volumetric analysis, neuronal population quantification, myelin reporter imaging, and behavioral assays","pmids":["38331943"],"confidence":"High","gaps":["Transcriptional program driving cerebellar phenotype not defined","Mechanism linking ZNF536 to myelin structure unknown","Direct neuronal targets in the valvular cerebelli not identified"]},{"year":2025,"claim":"Demonstrated conserved repressor function in human neurons and revealed a cis-regulatory dimension relevant to disease.","evidence":"CRISPR/Cas9 knockout and intronic deletion in SH-SY5Y cells with RNA-seq, GSEA, and neurite outgrowth measurement","pmids":["41163642"],"confidence":"High","gaps":["Direct vs. indirect regulation of RAR target genes not distinguished","Mechanism of E2F4 pathway dysregulation unresolved","Causal contribution of the intronic deletion to schizophrenia phenotypes not established"]},{"year":null,"claim":"The genome-wide direct target repertoire of ZNF536 and the co-regulatory machinery through which it represses RARE-mediated transcription remain undefined.","evidence":"No ChIP-based occupancy or co-repressor interaction data in the available corpus","pmids":[],"confidence":"Low","gaps":["No endogenous genomic binding sites mapped","No protein partners or co-repressors identified","Structural basis of DNA recognition not determined"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0003677","term_label":"DNA binding","supporting_discovery_ids":[2]},{"term_id":"GO:0140110","term_label":"transcription regulator activity","supporting_discovery_ids":[0,5]}],"localization":[{"term_id":"GO:0005634","term_label":"nucleus","supporting_discovery_ids":[0]}],"pathway":[{"term_id":"R-HSA-1266738","term_label":"Developmental Biology","supporting_discovery_ids":[0,3,4]},{"term_id":"R-HSA-74160","term_label":"Gene expression (Transcription)","supporting_discovery_ids":[0,5]}],"complexes":[],"partners":[],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"O15090","full_name":"Zinc finger protein 536","aliases":[],"length_aa":1300,"mass_kda":141.4,"function":"Transcriptional repressor that negatively regulates neuron differentiation by repressing retinoic acid-induced gene transcription (PubMed:19398580). Binds and interrupts RARA from binding to retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (PubMed:19398580). Recognizes and binds 2 copies of the core DNA sequence 5'-CCCCCA-3' (PubMed:14621294)","subcellular_location":"Nucleus","url":"https://www.uniprot.org/uniprotkb/O15090/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/ZNF536","classification":"Not Classified","n_dependent_lines":29,"n_total_lines":1208,"dependency_fraction":0.024006622516556293},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"CALM2","stoichiometry":0.2},{"gene":"CALM3","stoichiometry":0.2},{"gene":"DBNL","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/ZNF536","total_profiled":1310},"omim":[{"mim_id":"618037","title":"ZINC FINGER PROTEIN 536; ZNF536","url":"https://www.omim.org/entry/618037"},{"mim_id":"605784","title":"TWEETY FAMILY MEMBER 1; TTYH1","url":"https://www.omim.org/entry/605784"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Nucleoplasm","reliability":"Approved"},{"location":"Cell Junctions","reliability":"Approved"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in many","driving_tissues":[{"tissue":"brain","ntpm":30.4},{"tissue":"retina","ntpm":8.8}],"url":"https://www.proteinatlas.org/search/ZNF536"},"hgnc":{"alias_symbol":["KIAA0390"],"prev_symbol":[]},"alphafold":{"accession":"O15090","domains":[{"cath_id":"3.30.160.60","chopping":"126-185","consensus_level":"medium","plddt":82.0032,"start":126,"end":185},{"cath_id":"3.30.160.60","chopping":"749-805","consensus_level":"medium","plddt":84.8468,"start":749,"end":805}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/O15090","model_url":"https://alphafold.ebi.ac.uk/files/AF-O15090-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-O15090-F1-predicted_aligned_error_v6.png","plddt_mean":46.84},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=ZNF536","jax_strain_url":"https://www.jax.org/strain/search?query=ZNF536"},"sequence":{"accession":"O15090","fasta_url":"https://rest.uniprot.org/uniprotkb/O15090.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/O15090/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/O15090"}},"corpus_meta":[{"pmid":"30929901","id":"PMC_30929901","title":"Phenotypic 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Follicle Profiles of microRNAs and mRNAs to Reveal the Pattern Formation of Hu Sheep Lambskin.","date":"2022","source":"Genes","url":"https://pubmed.ncbi.nlm.nih.gov/35205386","citation_count":16,"is_preprint":false},{"pmid":"37460928","id":"PMC_37460928","title":"Genomic analyses of germline and somatic variation in high-grade serous ovarian cancer.","date":"2023","source":"Journal of ovarian research","url":"https://pubmed.ncbi.nlm.nih.gov/37460928","citation_count":10,"is_preprint":false},{"pmid":"21893220","id":"PMC_21893220","title":"A girl with an atypical form of ataxia telangiectasia and an additional de novo 3.14 Mb microduplication in region 19q12.","date":"2011","source":"European journal of medical genetics","url":"https://pubmed.ncbi.nlm.nih.gov/21893220","citation_count":10,"is_preprint":false},{"pmid":"38331943","id":"PMC_38331943","title":"Impairments of cerebellar structure and function in a zebrafish KO of neuropsychiatric risk gene 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It acts by inhibiting RA response element-mediated transcriptional activity, and overexpression of RA receptor alpha rescues the inhibitory effect of ZNF536.\",\n      \"method\": \"Overexpression and knockdown/mutation in P19 cells with RA-induced differentiation; RARE-reporter assays; rescue by RARα overexpression\",\n      \"journal\": \"Molecular and cellular biology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — multiple orthogonal methods (gain-of-function, loss-of-function, reporter assay, rescue experiment) in a single focused mechanistic study\",\n      \"pmids\": [\"19398580\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2009,\n      \"finding\": \"ZNF536 is predominantly expressed in the brain, specifically in the developing central nervous system (cerebral cortex, hippocampus, hypothalamic area, dorsal root ganglia), and its expression increases at early stages of RA-induced neuronal differentiation in P19 cells.\",\n      \"method\": \"Expression profiling during P19 differentiation; regional brain localization studies\",\n      \"journal\": \"Molecular and cellular biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — direct localization data from a single focused study, replicated conceptually across multiple papers\",\n      \"pmids\": [\"19398580\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2003,\n      \"finding\": \"The KIAA0390 protein (ZNF536 alias) binds specifically to the DNA core sequence CCCCC(A), the same consensus recognized by ZNF219, as demonstrated by random oligonucleotide selection assay and electromobility gel shift assay.\",\n      \"method\": \"Random oligonucleotide selection assay (SELEX) and electromobility gel shift assay (EMSA) with expressed KIAA0390 protein\",\n      \"journal\": \"DNA research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 1 / Weak — in vitro biochemical binding assay, single lab, single study\",\n      \"pmids\": [\"14621294\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"znf536 (zebrafish ortholog) is required for the development of forebrain neurons implicated in social behavior and stress, as revealed by single-cell RNA sequencing and mutant phenotyping in zebrafish.\",\n      \"method\": \"Zebrafish mutagenesis screen; whole-brain activity mapping; single-cell RNA sequencing; behavioral profiling\",\n      \"journal\": \"Cell\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — loss-of-function in zebrafish with defined anatomical and cellular phenotype, orthogonal methods (scRNA-seq + behavioral + brain imaging)\",\n      \"pmids\": [\"30929901\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"znf536 knockout zebrafish display decreased cerebellar volume with reduced populations of specific neuronal cells, especially in the valvular cerebelli (Va), and disorganization of a myelin structure within the Va, establishing ZNF536 as required for cerebellar development.\",\n      \"method\": \"CRISPR/Cas9 knockout zebrafish; brain volumetric analysis; transgenic myelin reporter line Tg[mbp:mgfp]; behavioral assays (anxiety, social interaction)\",\n      \"journal\": \"Translational psychiatry\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — clean KO with multiple orthogonal readouts (anatomy, cell populations, myelin structure, behavior) in a dedicated mechanistic study\",\n      \"pmids\": [\"38331943\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"CRISPR/Cas9 knockout of ZNF536 in SH-SY5Y cells impairs activation of retinoic acid receptor (RAR) target genes during ATRA-induced differentiation, reduces neurite outgrowth, causes failure of neuronal maturation, and dysregulates E2F4-mediated cell cycle pathways. An intronic deletion within ZNF536 also altered expression of multiple schizophrenia-associated genes, indicating cis-regulatory function.\",\n      \"method\": \"CRISPR/Cas9 dual-allelic knockout (103 kb intronic deletion + zinc finger domain disruption); transcriptome profiling (RNA-seq); GSEA; neurite outgrowth measurement\",\n      \"journal\": \"Frontiers in molecular neuroscience\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO with multiple orthogonal methods (transcriptomics, cell morphology, pathway analysis) in a single dedicated mechanistic study\",\n      \"pmids\": [\"41163642\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"ZNF536 is a brain-enriched C2H2 zinc finger transcriptional repressor that binds the DNA consensus sequence CCCCC(A) and negatively regulates neuronal differentiation by suppressing retinoic acid response element-mediated transcription; loss of ZNF536 in both zebrafish and human neuronal cell models impairs RAR target gene activation, reduces neurite outgrowth, disrupts cerebellar development (particularly in the valvular cerebelli), and affects forebrain neurons involved in social behavior and stress responses.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"ZNF536 is a brain-enriched C2H2 zinc finger transcription factor that acts as a negative regulator of neuronal differentiation [#0]. It binds the DNA core consensus CCCCC(A) [#2] and suppresses retinoic acid response element (RARE)-mediated transcription, with overexpression inhibiting RA-induced neuronal differentiation in P19 cells while depletion or mutation enhances it; restoring RAR\\u03b1 rescues this repressive effect, placing ZNF536 upstream of retinoic acid receptor signaling [#0]. Consistent with this role, ZNF536 loss in human SH-SY5Y neurons impairs activation of RAR target genes during ATRA-induced differentiation, reduces neurite outgrowth, blocks neuronal maturation, and dysregulates E2F4-linked cell cycle pathways [#5]. In zebrafish, loss of the znf536 ortholog disrupts development of forebrain neurons implicated in social behavior and stress [#3] and reduces cerebellar volume with loss of specific neuronal populations and myelin disorganization in the valvular cerebelli [#4], establishing a conserved requirement in brain development. The gene also harbors cis-regulatory sequence influencing expression of schizophrenia-associated genes [#5].\",\n  \"teleology\": [\n    {\n      \"year\": 2003,\n      \"claim\": \"Established the DNA sequence specificity of the ZNF536 protein, defining how it could engage target promoters.\",\n      \"evidence\": \"SELEX and EMSA with expressed KIAA0390 (ZNF536) protein\",\n      \"pmids\": [\"14621294\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"In vitro binding only; no endogenous genomic targets identified\", \"Functional consequence of CCCCC(A) binding not tested\", \"Overlap with ZNF219 specificity leaves target discrimination unresolved\"]\n    },\n    {\n      \"year\": 2009,\n      \"claim\": \"Defined ZNF536 as a repressor of RA-driven neuronal differentiation, linking its zinc finger activity to a specific developmental signaling pathway.\",\n      \"evidence\": \"Overexpression, knockdown/mutation, RARE-reporter assays, and RAR\\u03b1 rescue in RA-differentiated P19 cells; brain expression profiling\",\n      \"pmids\": [\"19398580\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Direct target genes repressed by ZNF536 not mapped\", \"Mechanism of RARE suppression (co-repressor recruitment vs. direct competition) unknown\", \"Link between CCCCC(A) binding and RARE regulation not established\"]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"Extended ZNF536 function to in vivo brain development, showing it is required for forebrain neurons governing social behavior and stress.\",\n      \"evidence\": \"Zebrafish mutagenesis, whole-brain activity mapping, single-cell RNA-seq, and behavioral profiling\",\n      \"pmids\": [\"30929901\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Molecular targets in forebrain neurons not identified\", \"Connection to RA signaling in vivo not tested\", \"Cell-autonomy of the requirement unresolved\"]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Pinpointed a discrete anatomical requirement for ZNF536 in cerebellar development and myelin organization.\",\n      \"evidence\": \"CRISPR/Cas9 knockout zebrafish with volumetric analysis, neuronal population quantification, myelin reporter imaging, and behavioral assays\",\n      \"pmids\": [\"38331943\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Transcriptional program driving cerebellar phenotype not defined\", \"Mechanism linking ZNF536 to myelin structure unknown\", \"Direct neuronal targets in the valvular cerebelli not identified\"]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Demonstrated conserved repressor function in human neurons and revealed a cis-regulatory dimension relevant to disease.\",\n      \"evidence\": \"CRISPR/Cas9 knockout and intronic deletion in SH-SY5Y cells with RNA-seq, GSEA, and neurite outgrowth measurement\",\n      \"pmids\": [\"41163642\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Direct vs. indirect regulation of RAR target genes not distinguished\", \"Mechanism of E2F4 pathway dysregulation unresolved\", \"Causal contribution of the intronic deletion to schizophrenia phenotypes not established\"]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The genome-wide direct target repertoire of ZNF536 and the co-regulatory machinery through which it represses RARE-mediated transcription remain undefined.\",\n      \"evidence\": \"No ChIP-based occupancy or co-repressor interaction data in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No endogenous genomic binding sites mapped\", \"No protein partners or co-repressors identified\", \"Structural basis of DNA recognition not determined\"]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003677\", \"supporting_discovery_ids\": [2]},\n      {\"term_id\": \"GO:0140110\", \"supporting_discovery_ids\": [0, 5]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-1266738\", \"supporting_discovery_ids\": [0, 3, 4]},\n      {\"term_id\": \"R-HSA-74160\", \"supporting_discovery_ids\": [0, 5]}\n    ],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":4,"faith_total":5,"faith_pct":80.0}}