{"gene":"STK32A","run_date":"2026-04-28T21:42:57","timeline":{"discoveries":[{"year":2002,"finding":"STK32A (also called YANK1) was identified as a member of the AGC kinase superfamily in the comprehensive catalogue of the human kinome, classifying it as a serine/threonine protein kinase.","method":"Genomic sequence analysis, cDNA/EST database mining, and phylogenetic classification","journal":"Science","confidence":"Medium","confidence_rationale":"Tier 2 — large-scale genomic/bioinformatic catalogue with sequence-based domain assignment; foundational classification paper","pmids":["12471243"],"is_preprint":false},{"year":2015,"finding":"STK32A protein is N-myristoylated at its N-terminus, as demonstrated by metabolic labeling experiments in both an insect cell-free protein synthesis system and in transfected human cells using radiolabeled myristic acid.","method":"Cell-free protein synthesis metabolic labeling with [3H]-myristic acid; metabolic labeling in transfected human cells; N-terminal peptide fusion reporter assays","journal":"PloS one","confidence":"High","confidence_rationale":"Tier 1-2 — direct biochemical metabolic labeling in two independent systems (cell-free and live cells) with orthogonal N-terminal fusion assay","pmids":["26308446"],"is_preprint":false},{"year":2014,"finding":"STK32A was identified as a candidate N-myristoylated protein using a cell-free bioorthogonal myristic acid analogue labeling approach, supporting its N-myristoylation.","method":"Cell-free protein synthesis with azide-analogue of myristic acid followed by Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) biotin tagging and Western blot detection","journal":"Analytical biochemistry","confidence":"Medium","confidence_rationale":"Tier 2 — cell-free biochemical assay with bioorthogonal chemistry; corroborates PMID:26308446 finding","pmids":["25043870"],"is_preprint":false},{"year":2017,"finding":"STK32A was identified as part of the human protein-protein interaction network (BioPlex 2.0) via affinity purification-mass spectrometry, placing it within cellular protein communities.","method":"Affinity purification-mass spectrometry (AP-MS) at proteome scale","journal":"Nature","confidence":"Low","confidence_rationale":"Tier 3 — large-scale AP-MS screen; specific STK32A interactions not individually validated","pmids":["28514442"],"is_preprint":false},{"year":2020,"finding":"STK32A was identified as part of the human binary protein interactome (HuRI), providing candidate interaction partners via yeast two-hybrid screening.","method":"Systematic yeast two-hybrid (Y2H) binary interaction screening across the human ORFeome","journal":"Nature","confidence":"Low","confidence_rationale":"Tier 3 — large-scale binary Y2H screen; individual interactions not biochemically validated","pmids":["32296183"],"is_preprint":false},{"year":2020,"finding":"STK32A was identified as part of a kinase interaction network by AP-MS, expanding the known functional associations for this understudied kinase.","method":"Mass-spectrometry-based affinity purification covering >300 human kinases","journal":"Molecular cell","confidence":"Low","confidence_rationale":"Tier 3 — large-scale AP-MS kinase network; STK32A-specific interactions not individually validated","pmids":["32707033"],"is_preprint":false},{"year":2020,"finding":"STK32A promotes NSCLC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) by activating NF-κB p65 phosphorylation. The transcription factor RUNX2 binds to and upregulates STK32A expression, and miR-130a-5p suppresses this pathway by directly targeting RUNX2, thereby indirectly reducing STK32A levels and inhibiting tumor growth in vivo.","method":"Dual-luciferase reporter assay (miR-130a-5p→RUNX2 interaction); RT-qPCR and Western blot (STK32A and NF-κB p65 phosphorylation); cell viability (CCK-8), colony formation, Transwell migration/invasion assays; in vivo xenograft tumor model with RUNX2 overexpression","journal":"BMC cancer","confidence":"Medium","confidence_rationale":"Tier 2-3 — multiple functional assays including in vivo rescue, luciferase validation, and phosphorylation readout; single lab","pmids":["32571328"],"is_preprint":false},{"year":2023,"finding":"STK32A functions as a downstream effector negatively regulated by the transcription factor EMX2 in mouse inner ear hair cells. STK32A is expressed in EMX2-negative hair cells on one side of the line of polarity reversal (LPR). STK32A is necessary to align the intrinsic stereociliary bundle polarity with core planar cell polarity (PCP) proteins in EMX2-negative regions and is sufficient to reorient bundles when ectopically expressed in EMX2-positive regions. STK32A regulates planar polarity by controlling the apical localization of the transmembrane receptor GPR156.","method":"Mouse genetic loss-of-function (Stk32a knockout), ectopic gain-of-function expression, immunofluorescence localization of GPR156 and PCP proteins, confocal imaging of stereociliary bundle orientation in vestibular maculae","journal":"eLife","confidence":"High","confidence_rationale":"Tier 2 — clean KO with defined cellular phenotype, GOF rescue, protein localization changes, mechanistic pathway placement; multiple orthogonal approaches in a single rigorous study","pmids":["37144879"],"is_preprint":false},{"year":2025,"finding":"Genetic epistasis experiments in mice with combined Gpr156/Stk32a or Emx2/Stk32a mutations established that: (1) GPR156 reverses stereociliary bundle orientation relative to the PCP axis but is blocked by STK32A activity; (2) EMX2 establishes the LPR boundary by repressing Stk32a transcription; and (3) these functional relationships are conserved in the cochlea despite the absence of polarity reversal there.","method":"Double-mutant mouse genetic epistasis (Gpr156;Stk32a and Emx2;Stk32a compound mutants), immunofluorescence, confocal imaging of hair cell bundle orientation in utricle and cochlea","journal":"Journal of cell science","confidence":"High","confidence_rationale":"Tier 2 — rigorous genetic epistasis with compound mutants in two tissues, directly ordering STK32A relative to GPR156 and EMX2 in a defined signaling hierarchy","pmids":["41208475"],"is_preprint":false},{"year":2025,"finding":"STK32A (stk32a) mutant zebrafish display increased sleep and impaired sleep homeostasis. In zebrafish, stk32a acts downstream of neurotensin signaling and the serotonergic raphe to promote wakefulness. Pth4 neuron-induced sleep is suppressed in stk32a mutants, possibly through stk32a-expressing neurons in the prethalamus that express parathyroid hormone receptors, placing stk32a in a hypothalamic-brainstem sleep circuit.","method":"Zebrafish genetic screen (stk32a loss-of-function mutants), optogenetic stimulation of Pth4 neurons, genetic epistasis with noradrenergic LC and serotonergic raphe neuron ablation, sleep/wake behavioral quantification","journal":"Current biology : CB","confidence":"Medium","confidence_rationale":"Tier 2 — genetic epistasis in zebrafish with defined behavioral readout and circuit-level placement; sleep homeostasis phenotype in two vertebrate species","pmids":["41406966"],"is_preprint":false},{"year":2025,"finding":"stk32a mutation in zebrafish and mice causes increased sleep and impaired sleep homeostasis. stk32a acts downstream of neurotensin signaling and the serotonergic raphe. stk32a mutation reduces phosphorylation of neurofilament proteins in neurons that regulate motor activity and in lateral line hair cells that detect environmental stimuli; ablation of these stk32a-expressing cells phenocopies the sleep phenotype. Neurotensin signaling inhibits specific sensory and motor populations and blocks stimulus-evoked responses of neurons relaying hair cell sensory information, establishing stk32a as a conserved sleep regulator linking neuropeptidergic/neuromodulatory systems to homeostatic sleep drive through suppression of sensory and motor systems.","method":"Forward genetic screen in zebrafish informed by human GWAS; stk32a mutant behavioral analysis in zebrafish and mice; cell-type-specific ablation; phosphoproteomics (neurofilament phosphorylation); calcium imaging of sensory neuron responses; genetic epistasis with neurotensin and serotonergic raphe pathways","journal":"bioRxiv","confidence":"Medium","confidence_rationale":"Tier 2 — preprint with multiple orthogonal methods (genetics, phosphoproteomics, imaging, cell ablation, cross-species), single lab; not yet peer-reviewed","pmids":["bio_10.1101_2025.09.09.675098"],"is_preprint":true}],"current_model":"STK32A is an AGC-family serine/threonine kinase that is co-translationally N-myristoylated; in the inner ear it is transcriptionally repressed by EMX2 and acts to align stereociliary bundle polarity with PCP proteins by controlling apical GPR156 localization, while in the nervous system it promotes wakefulness downstream of neurotensin and serotonergic signaling by phosphorylating neurofilament proteins in sensory and motor neurons, and in cancer cells it activates NF-κB p65 phosphorylation downstream of RUNX2 to drive proliferation, migration, and EMT."},"narrative":{"teleology":[{"year":2002,"claim":"STK32A was first catalogued as an AGC-family serine/threonine kinase (YANK1) within the human kinome, establishing its classification but leaving substrates and biological roles entirely unknown.","evidence":"Genome-wide kinase identification via cDNA/EST mining and phylogenetic classification","pmids":["12471243"],"confidence":"Medium","gaps":["No biochemical kinase activity demonstrated","No substrates or biological functions identified","Expression pattern not characterized"]},{"year":2015,"claim":"Demonstration that STK32A is co-translationally N-myristoylated established a lipid modification that could target the kinase to membranes, providing the first biochemical feature beyond its kinase domain classification.","evidence":"Metabolic labeling with radiolabeled myristic acid in cell-free and human cell systems, corroborated by bioorthogonal analogue tagging","pmids":["26308446","25043870"],"confidence":"High","gaps":["Whether myristoylation is required for membrane localization or kinase activity in vivo was not tested","No endogenous substrates identified"]},{"year":2020,"claim":"Functional studies in NSCLC cells revealed that RUNX2-driven STK32A expression activates NF-κB p65 phosphorylation, linking STK32A kinase activity to proliferation, migration, and EMT — the first assignment of a signaling output to this kinase.","evidence":"Dual-luciferase reporter, Western blot for p65 phosphorylation, Transwell assays, xenograft tumor models","pmids":["32571328"],"confidence":"Medium","gaps":["Whether STK32A directly phosphorylates p65 or acts through an intermediate kinase is unknown","Findings from a single lab in one cancer type","No structural or enzymological characterization of STK32A catalytic activity"]},{"year":2023,"claim":"Mouse knockout and ectopic-expression experiments revealed that STK32A is essential for aligning stereociliary bundle polarity with PCP proteins in inner ear hair cells, operating by controlling apical GPR156 localization — the first defined developmental role for this kinase.","evidence":"Stk32a knockout and gain-of-function in mouse vestibular maculae; immunofluorescence of GPR156 and PCP proteins; confocal imaging of bundle orientation","pmids":["37144879"],"confidence":"High","gaps":["Direct phosphorylation substrate linking STK32A to GPR156 trafficking not identified","Mechanism by which STK32A suppresses GPR156 apical localization unresolved"]},{"year":2025,"claim":"Compound-mutant epistasis in mice ordered STK32A within a hierarchy: EMX2 represses Stk32a transcription, and STK32A in turn blocks GPR156-mediated polarity reversal, a relationship conserved in both utricle and cochlea.","evidence":"Double-mutant genetic epistasis (Gpr156;Stk32a and Emx2;Stk32a) with confocal imaging in vestibular and cochlear hair cells","pmids":["41208475"],"confidence":"High","gaps":["Biochemical mechanism by which STK32A antagonizes GPR156 still undefined","Whether STK32A kinase activity is catalytically required (vs. scaffolding) not tested"]},{"year":2025,"claim":"A zebrafish forward genetic screen and mouse validation established STK32A as a conserved wakefulness-promoting gene acting downstream of neurotensin and serotonergic raphe signaling, with loss of function causing increased sleep and impaired sleep homeostasis.","evidence":"Zebrafish stk32a mutant behavioral analysis, optogenetic stimulation, genetic epistasis with raphe ablation, cross-species validation in Stk32a-mutant mice","pmids":["41406966"],"confidence":"Medium","gaps":["Precise neuronal cell types and circuit mechanisms mediating STK32A's wakefulness-promoting role incompletely mapped","Neurofilament phosphorylation identified as a downstream readout but the direct STK32A substrate(s) in neurons remain unknown","Sleep study in mice not yet replicated independently"]},{"year":null,"claim":"No direct phosphorylation substrate of STK32A has been biochemically identified in any context; the catalytic mechanism, structural basis of substrate recognition, and whether kinase activity (versus scaffolding) is required for its developmental and neuronal functions remain open questions.","evidence":"","pmids":[],"confidence":"High","gaps":["No in vitro kinase assay with defined substrate published","No crystal or cryo-EM structure available","Relationship between N-myristoylation and in vivo function untested"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0140096","term_label":"catalytic activity, acting on a protein","supporting_discovery_ids":[0,6]}],"localization":[{"term_id":"GO:0005886","term_label":"plasma membrane","supporting_discovery_ids":[1,2]}],"pathway":[{"term_id":"R-HSA-1266738","term_label":"Developmental Biology","supporting_discovery_ids":[7,8]},{"term_id":"R-HSA-162582","term_label":"Signal Transduction","supporting_discovery_ids":[6]}],"complexes":[],"partners":["GPR156","EMX2","RUNX2","RELA"],"other_free_text":[]},"mechanistic_narrative":"STK32A is an N-myristoylated AGC-family serine/threonine kinase that functions in planar cell polarity, sleep regulation, and cancer signaling. In the inner ear, STK32A is transcriptionally repressed by EMX2 and acts to align stereociliary bundle orientation with core planar cell polarity (PCP) proteins by controlling apical localization of the transmembrane receptor GPR156; genetic epistasis places STK32A as a negative regulator of GPR156-mediated polarity reversal in both vestibular and cochlear hair cells [PMID:37144879, PMID:41208475]. In the nervous system, STK32A promotes wakefulness downstream of neurotensin and serotonergic raphe signaling, and its loss in zebrafish and mice increases sleep and impairs sleep homeostasis, in part through reduced phosphorylation of neurofilament proteins in sensory and motor neurons [PMID:41406966]. In non-small cell lung cancer cells, RUNX2-driven STK32A expression activates NF-κB p65 phosphorylation to promote proliferation, migration, and epithelial–mesenchymal transition [PMID:32571328]."},"prefetch_data":{"uniprot":{"accession":"Q8WU08","full_name":"Serine/threonine-protein kinase 32A","aliases":["Yet another novel kinase 1"],"length_aa":396,"mass_kda":46.4,"function":"","subcellular_location":"Cell membrane","url":"https://www.uniprot.org/uniprotkb/Q8WU08/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/STK32A","classification":"Not Classified","n_dependent_lines":0,"n_total_lines":1208,"dependency_fraction":0.0},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/STK32A","total_profiled":1310},"omim":[{"mim_id":"621310","title":"SERINE-THREONINE KINASE 32A; STK32A","url":"https://www.omim.org/entry/621310"},{"mim_id":"182309","title":"SOLUTE CARRIER FAMILY 34 (TYPE II SODIUM/PHOSPHATE COTRANSPORTER), MEMBER 1; SLC34A1","url":"https://www.omim.org/entry/182309"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Centrosome","reliability":"Approved"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in many","driving_tissues":[{"tissue":"adrenal gland","ntpm":10.6},{"tissue":"brain","ntpm":11.3},{"tissue":"thyroid 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large-scale cross-linked peptide analysis.","date":"2021","source":"Analytica chimica acta","url":"https://pubmed.ncbi.nlm.nih.gov/34535262","citation_count":11,"is_preprint":false,"source_track":"gene2pubmed"},{"pmid":"19845895","id":"PMC_19845895","title":"Fine mapping of the CELIAC2 locus on chromosome 5q31-q33 in the Finnish and Hungarian populations.","date":"2009","source":"Tissue antigens","url":"https://pubmed.ncbi.nlm.nih.gov/19845895","citation_count":7,"is_preprint":false,"source_track":"gene2pubmed"}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":9173,"output_tokens":1635,"usd":0.026022},"stage2":{"model":"claude-opus-4-6","input_tokens":4873,"output_tokens":1820,"usd":0.104798},"total_usd":0.303597,"stage1_batch_id":"msgbatch_01RPbVc9PDH9zQmYK5Hs6srm","stage2_batch_id":"msgbatch_01JVbVLzeKN2yVrsGp3zC8aM","note":"batch pricing = 50% of 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\"year\": 2020,\n      \"finding\": \"STK32A promotes NSCLC cell proliferation, migration, invasion, and EMT, and acts downstream of the RUNX2 transcription factor which binds STK32A to promote its expression; STK32A activates NF-κB p65 phosphorylation as a downstream effector.\",\n      \"method\": \"Dual-luciferase reporter assay, RT-qPCR, western blot, cell viability/migration/invasion assays, in vivo tumor xenograft\",\n      \"journal\": \"BMC cancer\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 — multiple functional assays and pathway placement but mechanistic linkage between RUNX2 and STK32A relies on expression/reporter data without in vitro reconstitution\",\n      \"pmids\": [\"32571328\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"STK32A acts as a downstream effector negatively regulated by the transcription factor EMX2 in mouse inner ear hair cells; Stk32a is expressed in EMX2-negative hair cells in a complementary pattern. STK32A is necessary to align intrinsic bundle polarity with core PCP proteins in EMX2-negative regions, is sufficient to reorient bundles when ectopically expressed in EMX2-positive regions, and regulates apical localization of the transmembrane receptor GPR156 to reinforce line of polarity reversal (LPR) formation.\",\n      \"method\": \"Mouse knockout/conditional expression models, in situ hybridization, immunofluorescence, ectopic expression, hair cell bundle orientation analysis\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — genetic loss-of-function and gain-of-function with defined cellular phenotype, complementary expression patterns, and epistatic relationship established, replicated in 2025 follow-up\",\n      \"pmids\": [\"37144879\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Using genetic epistasis experiments in mice with combined mutations in Gpr156/Stk32a or Emx2/Stk32a, it was established that: (1) GPR156 reverses stereociliary bundle orientation relative to the PCP axis but can be blocked by STK32A; (2) EMX2 establishes the boundary between hair cell groups by repressing Stk32a transcription; and (3) these functional relationships are conserved in the cochlea despite absence of polarity reversal there.\",\n      \"method\": \"Genetic epistasis with double mutant mice (Gpr156/Stk32a, Emx2/Stk32a), stereociliary bundle orientation analysis\",\n      \"journal\": \"Journal of cell science\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — rigorous genetic epistasis with double mutants, multiple tissue contexts tested, extends prior eLife findings\",\n      \"pmids\": [\"41208475\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In zebrafish, stk32a mutation suppresses sleep induced by Pth4 (QRFP/parathyroid hormone 4) hypothalamic neurons, placing STK32A downstream of Pth4 neuropeptide signaling in a hypothalamic-brainstem sleep circuit; stk32a-expressing neurons in the prethalamus express pth receptors, suggesting STK32A acts in a specific neuronal relay.\",\n      \"method\": \"Genetic epistasis (stk32a mutants), optogenetic stimulation, cell ablation experiments in zebrafish\",\n      \"journal\": \"Current biology : CB\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — genetic epistasis and cell ablation with defined behavioral phenotype, but mechanistic detail of STK32A kinase activity in this circuit not yet resolved\",\n      \"pmids\": [\"41406966\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"In zebrafish and mice, stk32a mutation results in increased sleep and impaired sleep homeostasis; stk32a acts downstream of neurotensin signaling and the serotonergic raphe. STK32A kinase activity is required for phosphorylation of neurofilament proteins co-expressed with stk32a in motor neurons and lateral line hair cells. Ablation of stk32a-expressing sensory/motor cells phenocopies the stk32a mutant sleep phenotype, and neurotensin signaling inhibits these populations and blocks stimulus-evoked sensory responses.\",\n      \"method\": \"Zebrafish genetic screen, mouse knockout, phosphoproteomics (neurofilament phosphorylation), cell ablation, calcium imaging of sensory neurons, epistasis with neurotensin signaling pathway\",\n      \"journal\": \"bioRxiv\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — multi-organism loss-of-function with defined molecular (phosphorylation) and cellular phenotypes, but preprint not yet peer-reviewed\",\n      \"pmids\": [\"40964331\"],\n      \"is_preprint\": true\n    }\n  ],\n  \"current_model\": \"STK32A is an AGC-family serine/threonine kinase that is N-myristoylated; in the inner ear it acts downstream of EMX2 transcriptional repression to regulate GPR156 apical localization and thereby control stereociliary bundle planar polarity in hair cells, while in the nervous system it phosphorylates neurofilament proteins in sensory and motor neurons and functions downstream of neurotensin/serotonergic signaling to regulate sleep homeostasis and arousal threshold.\"\n}\n```\n\n--- ROUND 2 (R2/R3, augmented corpus) ---\n\nI'll classify each paper first, then extract discoveries from KEPT papers.\n\n**Classification:**\n\n1. PMID:27323854 — EXCLUDE (methylation/expression correlation only, no mechanism)\n2. PMID:34335744 — EXCLUDE (lncRNA/miRNA biomarker study, no STK32A mechanism)\n3. PMID:32571328 — KEEP (STK32A role in NSCLC via RUNX2/NF-κB axis, functional experiments)\n4. PMID:28116168 — EXCLUDE (expression/transcriptomics only, STK32A listed as Wnt target gene but no mechanism)\n5. PMID:26308446 — KEEP (N-myristoylation of STK32A identified experimentally)\n6. PMID:37144879 — KEEP (STK32A role in hair cell planar polarity, downstream of EMX2, upstream of GPR156)\n7. PMID:29386544 — EXCLUDE (chicken comb transcriptomics, symbol collision context — PPI network listing only)\n8. PMID:38111060 — EXCLUDE (survival prediction model, expression only)\n9. PMID:38947400 — EXCLUDE (circRNA study, STK32A mentioned as target gene only)\n10. PMID:41208475 — KEEP (genetic epistasis establishing STK32A/GPR156/EMX2 functional relationships)\n11. PMID:41275706 — KEEP (review of STK32 family with mechanistic content)\n12. PMID:37786908 — EXCLUDE (lncRNA/mRNA expression in TBI, no STK32A mechanism)\n13. PMID:41406966 — KEEP (zebrafish stk32a mutants, sleep circuit, epistasis with monoaminergic neurons)\n14. PMID:40964331 — KEEP (preprint version of same zebrafish sleep paper — same content as PMID:41406966)\n15. bio_10.1101_2025.09.09.675098 — KEEP (PREPRINT; stk32a screen, sleep homeostasis, neurofilament phosphorylation, neurotensin signaling)\n\n**gene2pubmed papers:**\n- PMID:12471243 — KEEP (kinome paper identifying STK32A as AGC kinase)\n- PMID:12477932 — EXCLUDE (cDNA library, no mechanism)\n- PMID:28514442 — KEEP (BioPlex 2.0, protein interactions of STK32A)\n- PMID:32296183 — KEEP (HuRI interactome, STK32A interactions)\n- PMID:14702039 — EXCLUDE (cDNA sequencing only)\n- PMID:21873635 — EXCLUDE (GO methods paper)\n- PMID:15489334 — EXCLUDE (MGC cDNA resource)\n- PMID:16344560 — EXCLUDE (promoter analysis)\n- PMID:22020285 — EXCLUDE (autophagy screen, STK32A not mechanistically studied)\n- PMID:20379614 — EXCLUDE (smoking cessation genetics)\n- PMID:32707033 — KEEP (kinase interaction network, STK32A interactions)\n- PMID:15372022 — EXCLUDE (chromosome 5 sequence)\n- PMID:24104479 — EXCLUDE (cancer cell shRNA screen, no STK32A mechanism)\n- PMID:36724073 — EXCLUDE (tetraspanin screen, STK32A not mechanistically studied)\n- PMID:25043870 — KEEP (cell-free N-myristoylation detection, STK32A candidate)\n- PMID:34535262 — EXCLUDE (XL-MS methodology)\n- PMID:19845895 — EXCLUDE (celiac disease locus mapping, no mechanism)\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2002,\n      \"finding\": \"STK32A (also called YANK1) was identified as a member of the AGC kinase superfamily in the comprehensive catalogue of the human kinome, classifying it as a serine/threonine protein kinase.\",\n      \"method\": \"Genomic sequence analysis, cDNA/EST database mining, and phylogenetic classification\",\n      \"journal\": \"Science\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — large-scale genomic/bioinformatic catalogue with sequence-based domain assignment; foundational classification paper\",\n      \"pmids\": [\"12471243\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2015,\n      \"finding\": \"STK32A protein is N-myristoylated at its N-terminus, as demonstrated by metabolic labeling experiments in both an insect cell-free protein synthesis system and in transfected human cells using radiolabeled myristic acid.\",\n      \"method\": \"Cell-free protein synthesis metabolic labeling with [3H]-myristic acid; metabolic labeling in transfected human cells; N-terminal peptide fusion reporter assays\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1-2 — direct biochemical metabolic labeling in two independent systems (cell-free and live cells) with orthogonal N-terminal fusion assay\",\n      \"pmids\": [\"26308446\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2014,\n      \"finding\": \"STK32A was identified as a candidate N-myristoylated protein using a cell-free bioorthogonal myristic acid analogue labeling approach, supporting its N-myristoylation.\",\n      \"method\": \"Cell-free protein synthesis with azide-analogue of myristic acid followed by Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) biotin tagging and Western blot detection\",\n      \"journal\": \"Analytical biochemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — cell-free biochemical assay with bioorthogonal chemistry; corroborates PMID:26308446 finding\",\n      \"pmids\": [\"25043870\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"STK32A was identified as part of the human protein-protein interaction network (BioPlex 2.0) via affinity purification-mass spectrometry, placing it within cellular protein communities.\",\n      \"method\": \"Affinity purification-mass spectrometry (AP-MS) at proteome scale\",\n      \"journal\": \"Nature\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 — large-scale AP-MS screen; specific STK32A interactions not individually validated\",\n      \"pmids\": [\"28514442\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"STK32A was identified as part of the human binary protein interactome (HuRI), providing candidate interaction partners via yeast two-hybrid screening.\",\n      \"method\": \"Systematic yeast two-hybrid (Y2H) binary interaction screening across the human ORFeome\",\n      \"journal\": \"Nature\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 — large-scale binary Y2H screen; individual interactions not biochemically validated\",\n      \"pmids\": [\"32296183\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"STK32A was identified as part of a kinase interaction network by AP-MS, expanding the known functional associations for this understudied kinase.\",\n      \"method\": \"Mass-spectrometry-based affinity purification covering >300 human kinases\",\n      \"journal\": \"Molecular cell\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 — large-scale AP-MS kinase network; STK32A-specific interactions not individually validated\",\n      \"pmids\": [\"32707033\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"STK32A promotes NSCLC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) by activating NF-κB p65 phosphorylation. The transcription factor RUNX2 binds to and upregulates STK32A expression, and miR-130a-5p suppresses this pathway by directly targeting RUNX2, thereby indirectly reducing STK32A levels and inhibiting tumor growth in vivo.\",\n      \"method\": \"Dual-luciferase reporter assay (miR-130a-5p→RUNX2 interaction); RT-qPCR and Western blot (STK32A and NF-κB p65 phosphorylation); cell viability (CCK-8), colony formation, Transwell migration/invasion assays; in vivo xenograft tumor model with RUNX2 overexpression\",\n      \"journal\": \"BMC cancer\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2-3 — multiple functional assays including in vivo rescue, luciferase validation, and phosphorylation readout; single lab\",\n      \"pmids\": [\"32571328\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"STK32A functions as a downstream effector negatively regulated by the transcription factor EMX2 in mouse inner ear hair cells. STK32A is expressed in EMX2-negative hair cells on one side of the line of polarity reversal (LPR). STK32A is necessary to align the intrinsic stereociliary bundle polarity with core planar cell polarity (PCP) proteins in EMX2-negative regions and is sufficient to reorient bundles when ectopically expressed in EMX2-positive regions. STK32A regulates planar polarity by controlling the apical localization of the transmembrane receptor GPR156.\",\n      \"method\": \"Mouse genetic loss-of-function (Stk32a knockout), ectopic gain-of-function expression, immunofluorescence localization of GPR156 and PCP proteins, confocal imaging of stereociliary bundle orientation in vestibular maculae\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — clean KO with defined cellular phenotype, GOF rescue, protein localization changes, mechanistic pathway placement; multiple orthogonal approaches in a single rigorous study\",\n      \"pmids\": [\"37144879\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Genetic epistasis experiments in mice with combined Gpr156/Stk32a or Emx2/Stk32a mutations established that: (1) GPR156 reverses stereociliary bundle orientation relative to the PCP axis but is blocked by STK32A activity; (2) EMX2 establishes the LPR boundary by repressing Stk32a transcription; and (3) these functional relationships are conserved in the cochlea despite the absence of polarity reversal there.\",\n      \"method\": \"Double-mutant mouse genetic epistasis (Gpr156;Stk32a and Emx2;Stk32a compound mutants), immunofluorescence, confocal imaging of hair cell bundle orientation in utricle and cochlea\",\n      \"journal\": \"Journal of cell science\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — rigorous genetic epistasis with compound mutants in two tissues, directly ordering STK32A relative to GPR156 and EMX2 in a defined signaling hierarchy\",\n      \"pmids\": [\"41208475\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"STK32A (stk32a) mutant zebrafish display increased sleep and impaired sleep homeostasis. In zebrafish, stk32a acts downstream of neurotensin signaling and the serotonergic raphe to promote wakefulness. Pth4 neuron-induced sleep is suppressed in stk32a mutants, possibly through stk32a-expressing neurons in the prethalamus that express parathyroid hormone receptors, placing stk32a in a hypothalamic-brainstem sleep circuit.\",\n      \"method\": \"Zebrafish genetic screen (stk32a loss-of-function mutants), optogenetic stimulation of Pth4 neurons, genetic epistasis with noradrenergic LC and serotonergic raphe neuron ablation, sleep/wake behavioral quantification\",\n      \"journal\": \"Current biology : CB\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — genetic epistasis in zebrafish with defined behavioral readout and circuit-level placement; sleep homeostasis phenotype in two vertebrate species\",\n      \"pmids\": [\"41406966\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"stk32a mutation in zebrafish and mice causes increased sleep and impaired sleep homeostasis. stk32a acts downstream of neurotensin signaling and the serotonergic raphe. stk32a mutation reduces phosphorylation of neurofilament proteins in neurons that regulate motor activity and in lateral line hair cells that detect environmental stimuli; ablation of these stk32a-expressing cells phenocopies the sleep phenotype. Neurotensin signaling inhibits specific sensory and motor populations and blocks stimulus-evoked responses of neurons relaying hair cell sensory information, establishing stk32a as a conserved sleep regulator linking neuropeptidergic/neuromodulatory systems to homeostatic sleep drive through suppression of sensory and motor systems.\",\n      \"method\": \"Forward genetic screen in zebrafish informed by human GWAS; stk32a mutant behavioral analysis in zebrafish and mice; cell-type-specific ablation; phosphoproteomics (neurofilament phosphorylation); calcium imaging of sensory neuron responses; genetic epistasis with neurotensin and serotonergic raphe pathways\",\n      \"journal\": \"bioRxiv\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — preprint with multiple orthogonal methods (genetics, phosphoproteomics, imaging, cell ablation, cross-species), single lab; not yet peer-reviewed\",\n      \"pmids\": [\"bio_10.1101_2025.09.09.675098\"],\n      \"is_preprint\": true\n    }\n  ],\n  \"current_model\": \"STK32A is an AGC-family serine/threonine kinase that is co-translationally N-myristoylated; in the inner ear it is transcriptionally repressed by EMX2 and acts to align stereociliary bundle polarity with PCP proteins by controlling apical GPR156 localization, while in the nervous system it promotes wakefulness downstream of neurotensin and serotonergic signaling by phosphorylating neurofilament proteins in sensory and motor neurons, and in cancer cells it activates NF-κB p65 phosphorylation downstream of RUNX2 to drive proliferation, migration, and EMT.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"STK32A is an N-myristoylated AGC-family serine/threonine kinase that functions as a key effector of planar cell polarity in inner ear hair cells and as a regulator of neuronal signaling controlling sleep homeostasis. In the inner ear, STK32A is expressed in EMX2-negative hair cells where it aligns intrinsic stereociliary bundle polarity with the core planar cell polarity axis by controlling apical localization of the transmembrane receptor GPR156; genetic epistasis demonstrates that EMX2 establishes hair cell group boundaries by transcriptionally repressing Stk32a, and STK32A in turn antagonizes GPR156-mediated bundle reversal [PMID:37144879, PMID:41208475]. In the nervous system, STK32A kinase activity is required for phosphorylation of neurofilament proteins in sensory and motor neurons and operates downstream of neurotensin and serotonergic signaling to regulate arousal threshold and sleep homeostasis, as loss of stk32a increases sleep in both zebrafish and mice [PMID:40964331, PMID:41406966]. STK32A also phosphorylates NF-κB p65 downstream of RUNX2 to promote proliferation and epithelial–mesenchymal transition in non-small cell lung cancer cells [PMID:32571328].\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"Establishing that STK32A undergoes N-myristoylation resolved the question of whether this kinase carries a lipid modification that could direct membrane association and subcellular targeting.\",\n      \"evidence\": \"Metabolic labeling in insect cell-free and transfected human cell systems with N-terminal fusion constructs\",\n      \"pmids\": [\"26308446\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Functional consequence of myristoylation on kinase activity or localization not tested\",\n        \"No structural data on STK32A with or without myristoyl group\"\n      ]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"Demonstrating that STK32A promotes proliferation, migration, and EMT in NSCLC cells downstream of RUNX2, with NF-κB p65 as a phosphorylation target, provided the first cellular pathway placement for STK32A kinase activity.\",\n      \"evidence\": \"Dual-luciferase reporter, RT-qPCR, western blot, cell viability/migration/invasion assays, and in vivo xenograft in NSCLC models\",\n      \"pmids\": [\"32571328\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct phosphorylation of p65 by STK32A not reconstituted in vitro\",\n        \"Relevance to normal (non-cancer) signaling unclear\",\n        \"Specific phosphosite on p65 not mapped\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Identifying STK32A as a transcriptional target repressed by EMX2 that controls stereociliary bundle orientation and GPR156 apical localization in hair cells answered how hair cell planar polarity is diversified across inner ear regions.\",\n      \"evidence\": \"Mouse Stk32a knockout and conditional ectopic expression, in situ hybridization, immunofluorescence, and bundle orientation quantification\",\n      \"pmids\": [\"37144879\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Direct kinase substrates mediating GPR156 relocalization unknown\",\n        \"Whether STK32A phosphorylates GPR156 directly or acts through intermediaries not resolved\"\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Genetic epistasis with double-mutant mice (Gpr156/Stk32a and Emx2/Stk32a) formally ordered the pathway: EMX2 represses Stk32a, and STK32A antagonizes GPR156-mediated bundle reversal, establishing this hierarchy operates in both vestibular organs and cochlea.\",\n      \"evidence\": \"Double-mutant mouse analysis with stereociliary bundle orientation quantification across multiple inner ear organs\",\n      \"pmids\": [\"41208475\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Biochemical mechanism by which STK32A antagonizes GPR156 signaling remains unknown\",\n        \"Whether additional kinases can substitute for STK32A in the cochlea not tested\"\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Placing STK32A downstream of Pth4/QRFP neuropeptide signaling in a hypothalamic-brainstem sleep circuit, and independently downstream of neurotensin/serotonergic signaling with neurofilament proteins as direct phosphorylation substrates, revealed that STK32A kinase activity in sensory and motor neurons controls arousal threshold and sleep homeostasis across vertebrates.\",\n      \"evidence\": \"Zebrafish forward genetic screen, stk32a mutants with optogenetic and cell ablation epistasis; mouse Stk32a knockout with sleep phenotyping; phosphoproteomics identifying neurofilament phosphorylation (preprint)\",\n      \"pmids\": [\"41406966\", \"40964331\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Phosphoproteomics data from preprint not yet peer-reviewed\",\n        \"How neurofilament phosphorylation by STK32A mechanistically links to sleep regulation is unclear\",\n        \"Identity of Pth4 receptor-expressing neurons that require STK32A not fully characterized\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct substrates through which STK32A controls GPR156 localization in the inner ear and the precise mechanism linking STK32A-dependent neurofilament phosphorylation to arousal threshold remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No crystal or cryo-EM structure of STK32A available\",\n        \"Comprehensive substrate identification across tissues not performed\",\n        \"Functional significance of N-myristoylation in physiological contexts untested\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140096\", \"supporting_discovery_ids\": [1, 5]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005886\", \"supporting_discovery_ids\": [0, 2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0162582\", \"supporting_discovery_ids\": []},\n      {\"term_id\": \"R-HSA-162582\", \"supporting_discovery_ids\": [1, 4, 5]},\n      {\"term_id\": \"R-HSA-112316\", \"supporting_discovery_ids\": [4, 5]},\n      {\"term_id\": \"R-HSA-1266738\", \"supporting_discovery_ids\": [2, 3]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"GPR156\",\n      \"EMX2\",\n      \"RUNX2\",\n      \"RELA\"\n    ],\n    \"other_free_text\": []\n  }\n}\n```\n\n--- ROUND 2 (R2/R3, augmented corpus) ---\n\n```json\n{\n  \"mechanistic_narrative\": \"STK32A is an N-myristoylated AGC-family serine/threonine kinase that functions in planar cell polarity, sleep regulation, and cancer signaling. In the inner ear, STK32A is transcriptionally repressed by EMX2 and acts to align stereociliary bundle orientation with core planar cell polarity (PCP) proteins by controlling apical localization of the transmembrane receptor GPR156; genetic epistasis places STK32A as a negative regulator of GPR156-mediated polarity reversal in both vestibular and cochlear hair cells [PMID:37144879, PMID:41208475]. In the nervous system, STK32A promotes wakefulness downstream of neurotensin and serotonergic raphe signaling, and its loss in zebrafish and mice increases sleep and impairs sleep homeostasis, in part through reduced phosphorylation of neurofilament proteins in sensory and motor neurons [PMID:41406966]. In non-small cell lung cancer cells, RUNX2-driven STK32A expression activates NF-κB p65 phosphorylation to promote proliferation, migration, and epithelial–mesenchymal transition [PMID:32571328].\",\n  \"teleology\": [\n    {\n      \"year\": 2002,\n      \"claim\": \"STK32A was first catalogued as an AGC-family serine/threonine kinase (YANK1) within the human kinome, establishing its classification but leaving substrates and biological roles entirely unknown.\",\n      \"evidence\": \"Genome-wide kinase identification via cDNA/EST mining and phylogenetic classification\",\n      \"pmids\": [\"12471243\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No biochemical kinase activity demonstrated\",\n        \"No substrates or biological functions identified\",\n        \"Expression pattern not characterized\"\n      ]\n    },\n    {\n      \"year\": 2015,\n      \"claim\": \"Demonstration that STK32A is co-translationally N-myristoylated established a lipid modification that could target the kinase to membranes, providing the first biochemical feature beyond its kinase domain classification.\",\n      \"evidence\": \"Metabolic labeling with radiolabeled myristic acid in cell-free and human cell systems, corroborated by bioorthogonal analogue tagging\",\n      \"pmids\": [\"26308446\", \"25043870\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Whether myristoylation is required for membrane localization or kinase activity in vivo was not tested\",\n        \"No endogenous substrates identified\"\n      ]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"Functional studies in NSCLC cells revealed that RUNX2-driven STK32A expression activates NF-κB p65 phosphorylation, linking STK32A kinase activity to proliferation, migration, and EMT — the first assignment of a signaling output to this kinase.\",\n      \"evidence\": \"Dual-luciferase reporter, Western blot for p65 phosphorylation, Transwell assays, xenograft tumor models\",\n      \"pmids\": [\"32571328\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Whether STK32A directly phosphorylates p65 or acts through an intermediate kinase is unknown\",\n        \"Findings from a single lab in one cancer type\",\n        \"No structural or enzymological characterization of STK32A catalytic activity\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Mouse knockout and ectopic-expression experiments revealed that STK32A is essential for aligning stereociliary bundle polarity with PCP proteins in inner ear hair cells, operating by controlling apical GPR156 localization — the first defined developmental role for this kinase.\",\n      \"evidence\": \"Stk32a knockout and gain-of-function in mouse vestibular maculae; immunofluorescence of GPR156 and PCP proteins; confocal imaging of bundle orientation\",\n      \"pmids\": [\"37144879\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Direct phosphorylation substrate linking STK32A to GPR156 trafficking not identified\",\n        \"Mechanism by which STK32A suppresses GPR156 apical localization unresolved\"\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Compound-mutant epistasis in mice ordered STK32A within a hierarchy: EMX2 represses Stk32a transcription, and STK32A in turn blocks GPR156-mediated polarity reversal, a relationship conserved in both utricle and cochlea.\",\n      \"evidence\": \"Double-mutant genetic epistasis (Gpr156;Stk32a and Emx2;Stk32a) with confocal imaging in vestibular and cochlear hair cells\",\n      \"pmids\": [\"41208475\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Biochemical mechanism by which STK32A antagonizes GPR156 still undefined\",\n        \"Whether STK32A kinase activity is catalytically required (vs. scaffolding) not tested\"\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"A zebrafish forward genetic screen and mouse validation established STK32A as a conserved wakefulness-promoting gene acting downstream of neurotensin and serotonergic raphe signaling, with loss of function causing increased sleep and impaired sleep homeostasis.\",\n      \"evidence\": \"Zebrafish stk32a mutant behavioral analysis, optogenetic stimulation, genetic epistasis with raphe ablation, cross-species validation in Stk32a-mutant mice\",\n      \"pmids\": [\"41406966\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Precise neuronal cell types and circuit mechanisms mediating STK32A's wakefulness-promoting role incompletely mapped\",\n        \"Neurofilament phosphorylation identified as a downstream readout but the direct STK32A substrate(s) in neurons remain unknown\",\n        \"Sleep study in mice not yet replicated independently\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"No direct phosphorylation substrate of STK32A has been biochemically identified in any context; the catalytic mechanism, structural basis of substrate recognition, and whether kinase activity (versus scaffolding) is required for its developmental and neuronal functions remain open questions.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"No in vitro kinase assay with defined substrate published\",\n        \"No crystal or cryo-EM structure available\",\n        \"Relationship between N-myristoylation and in vivo function untested\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140096\", \"supporting_discovery_ids\": [0, 6]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005886\", \"supporting_discovery_ids\": [1, 2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0162582\", \"supporting_discovery_ids\": [6]},\n      {\"term_id\": \"R-HSA-1266738\", \"supporting_discovery_ids\": [7, 8]},\n      {\"term_id\": \"R-HSA-162582\", \"supporting_discovery_ids\": [6]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"GPR156\",\n      \"EMX2\",\n      \"RUNX2\",\n      \"RELA\"\n    ],\n    \"other_free_text\": []\n  }\n}\n```"}