{"gene":"SSR1","run_date":"2026-06-10T07:46:41","timeline":{"discoveries":[{"year":2006,"finding":"TRAPalpha (SSR1) is required for efficient substrate-specific protein translocation across the endoplasmic reticulum membrane in vivo. Homozygous Trapalpha mutant mice (producing a truncated TRAPalpha fused to ShBle-beta-galactosidase) showed impaired protein secretion in transfected embryonic fibroblasts, confirming that the TRAP complex does not function properly without intact TRAPalpha. Mutant pups died at birth with severe cardiac defects including absence of outflow tract septation (double-outlet right ventricle), establishing that TRAPalpha plays a crucial role in mammalian heart development, likely by enabling translocation of factors required for endocardial cushion maturation.","method":"Gene targeting/knock-in mouse model producing truncated TRAPalpha; protein secretion assay in transfected embryonic fibroblasts; histological analysis of cardiac morphology","journal":"Molecular and cellular biology","confidence":"High","confidence_rationale":"Tier 2 / Moderate — clean KO/knock-in with defined cellular (secretion assay) and developmental phenotype, multiple orthogonal readouts (fibroblast secretion, cardiac histology) in a single rigorous study","pmids":["17015483"],"is_preprint":false},{"year":2021,"finding":"SSR1-derived peptides are presented on HLA class I in a TAP-independent manner. A T cell tool recognizing a TAP-independent SSR1 signal-sequence-derived antigen was established, and monitoring of multiple healthy cell types showed that most (but not all) healthy cells present this antigen under normal and inflammatory conditions, demonstrating that TAP-independent SSR1 antigen presentation is variable across cell types.","method":"T cell recognition assay using a T cell tool specific for a TAP-independent SSR1-derived HLA-I antigen; flow cytometry; testing across multiple healthy cell types under normal and inflammatory conditions","journal":"iScience","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — defined T cell tool with functional readout across multiple cell types, single lab, two orthogonal methods (antigen presentation assay + inflammatory stimulation)","pmids":["33554062"],"is_preprint":false},{"year":2024,"finding":"CircSSR1 (a circular RNA from the SSR1 locus) promotes SSR1 protein translation via m6A modification (through interaction with the m6A reader YTHDF1), thereby activating endoplasmic reticulum stress and leading to pyroptosis in pulmonary artery smooth muscle cells. Knockdown of circSSR1 inhibited hypoxia-induced PASMCs pyroptosis in vivo and in vitro. G3BP1 was identified as an upstream regulator that induces circSSR1 degradation under hypoxia. NOTE: This finding relates to a circRNA product of the SSR1 locus and its regulatory effect on SSR1 protein levels, not a direct mechanistic discovery about the SSR1 protein itself.","method":"RNA pull-down, RIP (RNA immunoprecipitation), Western blotting, PI staining, LDH release assay, RNA-FISH, qRT-PCR; in vivo and in vitro hypoxia models","journal":"Respiratory research","confidence":"Low","confidence_rationale":"Tier 3 / Weak — finding primarily concerns a circRNA regulatory layer upstream of SSR1 protein; the mechanistic claim about SSR1 protein itself (ERS activation) is inferred indirectly from circRNA overexpression, single lab","pmids":["39354535"],"is_preprint":false},{"year":2026,"finding":"The TRAP complex (SSR1, SSR2, SSR3, SSR4) is essential for protein translocation, folding, and quality control at the ER. SSR1 and SSR3 are described as essential for glucose metabolism and are associated with diabetes risk. TRAP subunit genes are upregulated in various cancers, and interaction between TRAP and ER stress pathways has been identified as a potential therapeutic target. NOTE: This is a review synthesizing evidence; mechanistic claims are attributed to the broader literature rather than new experiments.","method":"Review/synthesis; pan-cancer analysis; no new primary experiments described in the abstract","journal":"Annals of medicine","confidence":"Low","confidence_rationale":"Tier 4 / Weak — review paper with no new primary experiments; mechanistic claims are synthesized from existing literature without new experimental validation","pmids":["41649879"],"is_preprint":false}],"current_model":"SSR1 (TRAPalpha) encodes the alpha subunit of the translocon-associated protein (TRAP) complex at the ER membrane, where it is required for substrate-specific protein translocation across the ER; loss-of-function in mice causes impaired protein secretion in fibroblasts and lethal cardiac outflow tract defects, while SSR1-derived peptides are presented on HLA class I via a TAP-independent pathway in healthy cells."},"narrative":{"mechanistic_narrative":"SSR1 (TRAPalpha) encodes the alpha subunit of the ER membrane translocon-associated protein (TRAP) complex and is required for efficient substrate-specific protein translocation across the endoplasmic reticulum membrane in vivo [PMID:17015483]. Loss of intact TRAPalpha impairs protein secretion in embryonic fibroblasts and causes lethal cardiac outflow tract defects in mice, including absent outflow tract septation, linking TRAP-dependent translocation to mammalian heart development [PMID:17015483]. Beyond its translocation role, an SSR1 signal-sequence-derived peptide is presented on HLA class I through a TAP-independent pathway, with presentation varying across healthy cell types under normal and inflammatory conditions [PMID:33554062]. No further mechanistic detail about the SSR1 protein itself has been characterized in the available corpus.","teleology":[{"year":2006,"claim":"Established that SSR1/TRAPalpha is functionally required for ER protein translocation in vivo and links this molecular role to a developmental phenotype, answering whether the TRAP complex can operate without intact TRAPalpha.","evidence":"Gene-targeted/knock-in mouse producing truncated TRAPalpha, with fibroblast secretion assay and cardiac histology","pmids":["17015483"],"confidence":"High","gaps":["Specific secreted substrates whose mistranslocation drives the cardiac defect were not identified","Molecular contribution of TRAPalpha within the TRAP complex (vs. other subunits) not dissected","No structural mechanism of substrate selectivity provided"]},{"year":2021,"claim":"Demonstrated that an SSR1 signal-sequence-derived peptide can reach HLA class I independently of TAP, revealing a non-canonical antigen-presentation route and its cell-type variability.","evidence":"T cell tool specific for a TAP-independent SSR1-derived HLA-I antigen, with flow cytometry across multiple healthy cell types under normal and inflammatory conditions","pmids":["33554062"],"confidence":"Medium","gaps":["Mechanism generating the TAP-independent peptide not defined","Functional/immunological consequence of presentation variability unknown","Relationship between SSR1 translocation function and its antigen generation unaddressed"]},{"year":2024,"claim":"Addressed an upstream regulatory layer, indicating that a circular RNA from the SSR1 locus can modulate SSR1 protein translation and ER stress in pulmonary artery smooth muscle cells.","evidence":"RNA pull-down, RIP, RNA-FISH, Western blotting and hypoxia models in vitro and in vivo","pmids":["39354535"],"confidence":"Low","gaps":["The mechanistic claim concerns circSSR1 regulation, not the SSR1 protein directly; ER-stress activation by SSR1 is inferred indirectly","Single lab without independent confirmation","Direct role of SSR1 protein in pyroptosis not separated from circRNA effects"]},{"year":2026,"claim":"Synthesized existing evidence positioning the full TRAP complex in ER translocation, folding, and quality control, and proposed associations with glucose metabolism, diabetes risk, and cancer.","evidence":"Review/synthesis with pan-cancer analysis; no new primary experiments","pmids":["41649879"],"confidence":"Low","gaps":["No new primary experimental validation; claims drawn from broader literature","Causal mechanism linking SSR1 to glucose metabolism or cancer not established","Therapeutic targeting of TRAP-ER stress interaction untested"]},{"year":null,"claim":"The specific protein substrates whose translocation depends on TRAPalpha, and the structural basis for its substrate selectivity, remain undefined.","evidence":"","pmids":[],"confidence":"High","gaps":["No substrate repertoire of TRAPalpha-dependent translocation identified","No structural model of TRAPalpha within the translocon","Mechanistic link between translocation defect and cardiac developmental phenotype unresolved"]}],"mechanism_profile":{"molecular_activity":[],"localization":[{"term_id":"GO:0005783","term_label":"endoplasmic reticulum","supporting_discovery_ids":[0]}],"pathway":[{"term_id":"R-HSA-392499","term_label":"Metabolism of proteins","supporting_discovery_ids":[0]}],"complexes":["TRAP complex"],"partners":[],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"P43307","full_name":"Translocon-associated protein subunit alpha","aliases":["Signal sequence receptor subunit alpha","SSR-alpha"],"length_aa":286,"mass_kda":32.2,"function":"TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins","subcellular_location":"Endoplasmic reticulum membrane","url":"https://www.uniprot.org/uniprotkb/P43307/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/SSR1","classification":"Not Classified","n_dependent_lines":33,"n_total_lines":1208,"dependency_fraction":0.027317880794701987},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"CTCF","stoichiometry":4.0},{"gene":"RPL13","stoichiometry":4.0},{"gene":"RPL19","stoichiometry":4.0},{"gene":"RPL35","stoichiometry":4.0},{"gene":"RPL4","stoichiometry":4.0},{"gene":"SEC61B","stoichiometry":4.0},{"gene":"BCAP31","stoichiometry":0.2},{"gene":"CANX","stoichiometry":0.2},{"gene":"CAPRIN1","stoichiometry":0.2},{"gene":"DDOST","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/SSR1","total_profiled":1310},"omim":[{"mim_id":"614138","title":"TRAFFICKING PROTEIN PARTICLE COMPLEX, SUBUNIT 11; TRAPPC11","url":"https://www.omim.org/entry/614138"},{"mim_id":"612582","title":"CHROMOSOME 6pter-p24 DELETION SYNDROME","url":"https://www.omim.org/entry/612582"},{"mim_id":"606213","title":"SIGNAL SEQUENCE RECEPTOR, GAMMA; SSR3","url":"https://www.omim.org/entry/606213"},{"mim_id":"600868","title":"SIGNAL SEQUENCE RECEPTOR, ALPHA; SSR1","url":"https://www.omim.org/entry/600868"},{"mim_id":"600867","title":"SIGNAL SEQUENCE RECEPTOR, BETA; SSR2","url":"https://www.omim.org/entry/600867"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Supported","locations":[{"location":"Endoplasmic reticulum","reliability":"Supported"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/SSR1"},"hgnc":{"alias_symbol":["TRAPA"],"prev_symbol":[]},"alphafold":{"accession":"P43307","domains":[{"cath_id":"2.60.40.10","chopping":"84-198","consensus_level":"high","plddt":95.0776,"start":84,"end":198}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/P43307","model_url":"https://alphafold.ebi.ac.uk/files/AF-P43307-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-P43307-F1-predicted_aligned_error_v6.png","plddt_mean":76.12},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=SSR1","jax_strain_url":"https://www.jax.org/strain/search?query=SSR1"},"sequence":{"accession":"P43307","fasta_url":"https://rest.uniprot.org/uniprotkb/P43307.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/P43307/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/P43307"}},"corpus_meta":[{"pmid":"21736910","id":"PMC_21736910","title":"Identification of an antifungal peptide 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Part B, Resources","url":"https://pubmed.ncbi.nlm.nih.gov/33366009","citation_count":2,"is_preprint":false},{"pmid":"35519496","id":"PMC_35519496","title":"Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa.","date":"2019","source":"RSC advances","url":"https://pubmed.ncbi.nlm.nih.gov/35519496","citation_count":2,"is_preprint":false},{"pmid":"31742429","id":"PMC_31742429","title":"Protective and Modulatory Effects of Trapa bispinosa and Trigonella foenum-graecum on Neuroblastoma Cells Through Neuronal Nitric Oxide Synthase.","date":"2019","source":"Assay and drug development technologies","url":"https://pubmed.ncbi.nlm.nih.gov/31742429","citation_count":1,"is_preprint":false},{"pmid":"38333092","id":"PMC_38333092","title":"Origin of domesticated water chestnuts (Trapa bispinosa Roxb.) and genetic variation in wild water chestnuts.","date":"2024","source":"Ecology and evolution","url":"https://pubmed.ncbi.nlm.nih.gov/38333092","citation_count":1,"is_preprint":false},{"pmid":"37511522","id":"PMC_37511522","title":"Trapa bispinosa Roxb. Pericarp Extract Exerts 5α-Reductase Inhibitory Activity in Castrated Benign Prostatic Hyperplasia Model Mice.","date":"2023","source":"International journal of molecular sciences","url":"https://pubmed.ncbi.nlm.nih.gov/37511522","citation_count":1,"is_preprint":false},{"pmid":"39886549","id":"PMC_39886549","title":"Trapa natans L. Extract Attenuates Inflammation and Oxidative Damage in Cisplatin-Induced Cardiotoxicity in Rats by Promoting M2 Macrophage Polarization.","date":"2025","source":"Mediators of inflammation","url":"https://pubmed.ncbi.nlm.nih.gov/39886549","citation_count":1,"is_preprint":false},{"pmid":"41649879","id":"PMC_41649879","title":"The TRAP complex (SSR1-SSR4): mechanistic roles and therapeutic opportunities.","date":"2026","source":"Annals of medicine","url":"https://pubmed.ncbi.nlm.nih.gov/41649879","citation_count":0,"is_preprint":false},{"pmid":"40199161","id":"PMC_40199161","title":"The functional conservation of SSR1 homologs in plants.","date":"2025","source":"Plant physiology and biochemistry : PPB","url":"https://pubmed.ncbi.nlm.nih.gov/40199161","citation_count":0,"is_preprint":false},{"pmid":"41550252","id":"PMC_41550252","title":"Inhibitory effects of water chestnut (Trapa bispinosa Roxb.) extract plus lutein on the progression of cataract and their impact on the retina and aorta in diabetic rats.","date":"2026","source":"Journal of diabetes and metabolic disorders","url":"https://pubmed.ncbi.nlm.nih.gov/41550252","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":30996,"output_tokens":1338,"usd":0.056529,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":8053,"output_tokens":1745,"usd":0.041945,"stage2_stop_reason":"end_turn"},"total_usd":0.098474,"stage1_batch_id":"msgbatch_013LWZZT6MWaqB9qyTSeQBmU","stage2_batch_id":"msgbatch_0194ZzQaTGNpruoNJBbEMbt3","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2006,\n      \"finding\": \"TRAPalpha (SSR1) is required for efficient substrate-specific protein translocation across the endoplasmic reticulum membrane in vivo. Homozygous Trapalpha mutant mice (producing a truncated TRAPalpha fused to ShBle-beta-galactosidase) showed impaired protein secretion in transfected embryonic fibroblasts, confirming that the TRAP complex does not function properly without intact TRAPalpha. Mutant pups died at birth with severe cardiac defects including absence of outflow tract septation (double-outlet right ventricle), establishing that TRAPalpha plays a crucial role in mammalian heart development, likely by enabling translocation of factors required for endocardial cushion maturation.\",\n      \"method\": \"Gene targeting/knock-in mouse model producing truncated TRAPalpha; protein secretion assay in transfected embryonic fibroblasts; histological analysis of cardiac morphology\",\n      \"journal\": \"Molecular and cellular biology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO/knock-in with defined cellular (secretion assay) and developmental phenotype, multiple orthogonal readouts (fibroblast secretion, cardiac histology) in a single rigorous study\",\n      \"pmids\": [\"17015483\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"SSR1-derived peptides are presented on HLA class I in a TAP-independent manner. A T cell tool recognizing a TAP-independent SSR1 signal-sequence-derived antigen was established, and monitoring of multiple healthy cell types showed that most (but not all) healthy cells present this antigen under normal and inflammatory conditions, demonstrating that TAP-independent SSR1 antigen presentation is variable across cell types.\",\n      \"method\": \"T cell recognition assay using a T cell tool specific for a TAP-independent SSR1-derived HLA-I antigen; flow cytometry; testing across multiple healthy cell types under normal and inflammatory conditions\",\n      \"journal\": \"iScience\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — defined T cell tool with functional readout across multiple cell types, single lab, two orthogonal methods (antigen presentation assay + inflammatory stimulation)\",\n      \"pmids\": [\"33554062\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"CircSSR1 (a circular RNA from the SSR1 locus) promotes SSR1 protein translation via m6A modification (through interaction with the m6A reader YTHDF1), thereby activating endoplasmic reticulum stress and leading to pyroptosis in pulmonary artery smooth muscle cells. Knockdown of circSSR1 inhibited hypoxia-induced PASMCs pyroptosis in vivo and in vitro. G3BP1 was identified as an upstream regulator that induces circSSR1 degradation under hypoxia. NOTE: This finding relates to a circRNA product of the SSR1 locus and its regulatory effect on SSR1 protein levels, not a direct mechanistic discovery about the SSR1 protein itself.\",\n      \"method\": \"RNA pull-down, RIP (RNA immunoprecipitation), Western blotting, PI staining, LDH release assay, RNA-FISH, qRT-PCR; in vivo and in vitro hypoxia models\",\n      \"journal\": \"Respiratory research\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — finding primarily concerns a circRNA regulatory layer upstream of SSR1 protein; the mechanistic claim about SSR1 protein itself (ERS activation) is inferred indirectly from circRNA overexpression, single lab\",\n      \"pmids\": [\"39354535\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"The TRAP complex (SSR1, SSR2, SSR3, SSR4) is essential for protein translocation, folding, and quality control at the ER. SSR1 and SSR3 are described as essential for glucose metabolism and are associated with diabetes risk. TRAP subunit genes are upregulated in various cancers, and interaction between TRAP and ER stress pathways has been identified as a potential therapeutic target. NOTE: This is a review synthesizing evidence; mechanistic claims are attributed to the broader literature rather than new experiments.\",\n      \"method\": \"Review/synthesis; pan-cancer analysis; no new primary experiments described in the abstract\",\n      \"journal\": \"Annals of medicine\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — review paper with no new primary experiments; mechanistic claims are synthesized from existing literature without new experimental validation\",\n      \"pmids\": [\"41649879\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"SSR1 (TRAPalpha) encodes the alpha subunit of the translocon-associated protein (TRAP) complex at the ER membrane, where it is required for substrate-specific protein translocation across the ER; loss-of-function in mice causes impaired protein secretion in fibroblasts and lethal cardiac outflow tract defects, while SSR1-derived peptides are presented on HLA class I via a TAP-independent pathway in healthy cells.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"SSR1 (TRAPalpha) encodes the alpha subunit of the ER membrane translocon-associated protein (TRAP) complex and is required for efficient substrate-specific protein translocation across the endoplasmic reticulum membrane in vivo [#0]. Loss of intact TRAPalpha impairs protein secretion in embryonic fibroblasts and causes lethal cardiac outflow tract defects in mice, including absent outflow tract septation, linking TRAP-dependent translocation to mammalian heart development [#0]. Beyond its translocation role, an SSR1 signal-sequence-derived peptide is presented on HLA class I through a TAP-independent pathway, with presentation varying across healthy cell types under normal and inflammatory conditions [#1]. No further mechanistic detail about the SSR1 protein itself has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2006,\n      \"claim\": \"Established that SSR1/TRAPalpha is functionally required for ER protein translocation in vivo and links this molecular role to a developmental phenotype, answering whether the TRAP complex can operate without intact TRAPalpha.\",\n      \"evidence\": \"Gene-targeted/knock-in mouse producing truncated TRAPalpha, with fibroblast secretion assay and cardiac histology\",\n      \"pmids\": [\"17015483\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Specific secreted substrates whose mistranslocation drives the cardiac defect were not identified\",\n        \"Molecular contribution of TRAPalpha within the TRAP complex (vs. other subunits) not dissected\",\n        \"No structural mechanism of substrate selectivity provided\"\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"Demonstrated that an SSR1 signal-sequence-derived peptide can reach HLA class I independently of TAP, revealing a non-canonical antigen-presentation route and its cell-type variability.\",\n      \"evidence\": \"T cell tool specific for a TAP-independent SSR1-derived HLA-I antigen, with flow cytometry across multiple healthy cell types under normal and inflammatory conditions\",\n      \"pmids\": [\"33554062\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Mechanism generating the TAP-independent peptide not defined\",\n        \"Functional/immunological consequence of presentation variability unknown\",\n        \"Relationship between SSR1 translocation function and its antigen generation unaddressed\"\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Addressed an upstream regulatory layer, indicating that a circular RNA from the SSR1 locus can modulate SSR1 protein translation and ER stress in pulmonary artery smooth muscle cells.\",\n      \"evidence\": \"RNA pull-down, RIP, RNA-FISH, Western blotting and hypoxia models in vitro and in vivo\",\n      \"pmids\": [\"39354535\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"The mechanistic claim concerns circSSR1 regulation, not the SSR1 protein directly; ER-stress activation by SSR1 is inferred indirectly\",\n        \"Single lab without independent confirmation\",\n        \"Direct role of SSR1 protein in pyroptosis not separated from circRNA effects\"\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Synthesized existing evidence positioning the full TRAP complex in ER translocation, folding, and quality control, and proposed associations with glucose metabolism, diabetes risk, and cancer.\",\n      \"evidence\": \"Review/synthesis with pan-cancer analysis; no new primary experiments\",\n      \"pmids\": [\"41649879\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No new primary experimental validation; claims drawn from broader literature\",\n        \"Causal mechanism linking SSR1 to glucose metabolism or cancer not established\",\n        \"Therapeutic targeting of TRAP-ER stress interaction untested\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific protein substrates whose translocation depends on TRAPalpha, and the structural basis for its substrate selectivity, remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"No substrate repertoire of TRAPalpha-dependent translocation identified\",\n        \"No structural model of TRAPalpha within the translocon\",\n        \"Mechanistic link between translocation defect and cardiac developmental phenotype unresolved\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0005783\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-392499\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\"TRAP complex\"],\n    \"partners\": [],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":3,"faith_total":3,"faith_pct":100.0}}