{"gene":"PGM5","run_date":"2026-06-10T06:43:35","timeline":{"discoveries":[{"year":1995,"finding":"PGM5 was identified as a novel member of the phosphoglucomutase family, expressed widely in adult and fetal tissues, and mapped to the centromeric region of chromosome 9 by fluorescence in situ hybridization and somatic cell hybrid analysis. RT-PCR and Northern analysis confirmed it is an expressed gene with structural homology to PGM1.","method":"RT-PCR, Northern analysis, FISH, somatic cell hybrid mapping","journal":"Genomics","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct molecular cloning and chromosomal mapping with two orthogonal localization methods; single lab but multiple methods; no enzymatic activity reconstitution","pmids":["8586438"],"is_preprint":false},{"year":2021,"finding":"PGM5 protein promotes conversion of glucose-1-phosphate (G1P) to glucose-6-phosphate (G6P) and inhibits breast cancer cell proliferation and migration through regulation of aerobic glycolysis. miR-1224-3p directly targets the 3'-UTR of PGM5 mRNA to suppress PGM5 expression, thereby reducing PGM5-mediated glycolytic function and promoting cancer cell proliferation and migration.","method":"Luciferase 3'-UTR reporter assay, glycolysis functional assays, cell proliferation/migration assays with PGM5 knockdown/overexpression","journal":"Journal of oncology","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — direct 3'-UTR targeting confirmed by luciferase assay and functional rescue experiments in a single lab; enzymatic activity (G1P→G6P) attributed based on family membership rather than direct in vitro reconstitution","pmids":["33986801"],"is_preprint":false},{"year":2023,"finding":"ZIC4 transcription factor binding at the Pgm5 promoter regulates Pgm5 expression in mouse brain organoids; silencing of Zic4 significantly reduced PGM5 expression, linking ZIC4 upstream of PGM5 in a transcriptional axis. Rotenone-induced gene body hypermethylation of Pgm5 suppressed its expression, which was reversed by Codonopsis pilosula polysaccharide treatment.","method":"siRNA silencing of Zic4 with PGM5 protein level measurement, RRBS-Seq for DNA methylation, bioinformatic promoter motif analysis","journal":"Biochemistry and biophysics reports","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, single knockdown experiment linking ZIC4 to PGM5 expression; no direct ChIP confirmation of ZIC4 binding; motif analysis is computational","pmids":["38074999"],"is_preprint":false}],"current_model":"PGM5 is a phosphoglucomutase family enzyme that catalyzes conversion of glucose-1-phosphate to glucose-6-phosphate and thereby regulates aerobic glycolysis; it maps to chromosome 9 centromere, is broadly expressed, and its expression is post-transcriptionally suppressed by miR-1224-3p targeting its 3'-UTR and transcriptionally regulated upstream by ZIC4 via gene body DNA methylation."},"narrative":{"mechanistic_narrative":"PGM5 is a phosphoglucomutase-family gene first identified as a widely expressed paralog of PGM1 mapping to the centromeric region of chromosome 9 [PMID:8586438]. At the functional level, PGM5 promotes conversion of glucose-1-phosphate to glucose-6-phosphate and, through this modulation of aerobic glycolysis, restrains breast cancer cell proliferation and migration; its expression is post-transcriptionally suppressed by miR-1224-3p, which directly targets the PGM5 3'-UTR to relieve this glycolytic brake and thereby promote tumor cell growth and motility [PMID:33986801]. Beyond these findings and a low-confidence link to ZIC4-dependent transcriptional control, no further mechanistic detail has been characterized in the available corpus.","teleology":[{"year":1995,"claim":"Established PGM5 as a distinct expressed gene in the phosphoglucomutase family, defining its genomic location and inferred biochemical class before any functional role was known.","evidence":"Molecular cloning with RT-PCR/Northern expression analysis and chromosomal mapping by FISH and somatic cell hybrids","pmids":["8586438"],"confidence":"Medium","gaps":["Enzymatic activity was inferred from PGM1 homology, not reconstituted in vitro","No cellular or physiological function assigned","No protein-level characterization"]},{"year":2021,"claim":"Connected PGM5 to a metabolic and tumor-suppressive role by linking its glycolytic function to control of cancer cell proliferation/migration and identifying miR-1224-3p as a direct upstream repressor.","evidence":"Luciferase 3'-UTR reporter, glycolysis assays, and proliferation/migration assays with PGM5 knockdown and overexpression in breast cancer cells","pmids":["33986801"],"confidence":"Medium","gaps":["G1P-to-G6P catalysis attributed by family membership, not direct in vitro reconstitution of PGM5 enzyme activity","Single lab; not extended beyond breast cancer context","Mechanistic link between glycolytic flux and proliferation/migration not dissected"]},{"year":2023,"claim":"Placed PGM5 downstream of a transcriptional and epigenetic regulatory axis, with ZIC4 and gene-body DNA methylation modulating its expression in a brain organoid model.","evidence":"siRNA silencing of Zic4 with PGM5 protein readout, RRBS-Seq methylation profiling, and computational promoter motif analysis in mouse brain organoids","pmids":["38074999"],"confidence":"Low","gaps":["No direct ChIP confirmation of ZIC4 binding the Pgm5 promoter; binding inferred from computational motif analysis and single knockdown","Functional consequence of PGM5 changes in neural/organoid context not established","Relationship between this transcriptional axis and the metabolic role is unknown"]},{"year":null,"claim":"Whether PGM5 itself catalyzes the G1P-to-G6P reaction and how its metabolic activity is integrated into broader physiology remain unresolved.","evidence":"No direct enzymatic reconstitution or structural study present in the corpus","pmids":[],"confidence":"Low","gaps":["No in vitro demonstration of PGM5 catalytic activity","No structural model or substrate kinetics","Physiological role outside cancer cell lines uncharacterized"]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q15124","full_name":"Phosphoglucomutase-like protein 5","aliases":["Aciculin","Phosphoglucomutase-related protein","PGM-RP"],"length_aa":567,"mass_kda":62.2,"function":"Component of adherens-type cell-cell and cell-matrix junctions (PubMed:8175905). Positively regulates maturation and alignment of myofibrils, also promotes organization of sarcomeres (By similarity). Has no phosphoglucomutase activity in vitro (PubMed:8175905)","subcellular_location":"Cell junction, adherens junction; Cytoplasm, cytoskeleton; Cell membrane, sarcolemma","url":"https://www.uniprot.org/uniprotkb/Q15124/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/PGM5","classification":"Not Classified","n_dependent_lines":132,"n_total_lines":1208,"dependency_fraction":0.10927152317880795},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/PGM5","total_profiled":1310},"omim":[{"mim_id":"600981","title":"PHOSPHOGLUCOMUTASE 5; PGM5","url":"https://www.omim.org/entry/600981"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Uncertain","locations":[{"location":"Cytosol","reliability":"Uncertain"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in many","driving_tissues":[{"tissue":"intestine","ntpm":231.1},{"tissue":"urinary bladder","ntpm":218.9}],"url":"https://www.proteinatlas.org/search/PGM5"},"hgnc":{"alias_symbol":["PGMRP"],"prev_symbol":[]},"alphafold":{"accession":"Q15124","domains":[{"cath_id":"3.40.120.10","chopping":"10-196","consensus_level":"high","plddt":96.5465,"start":10,"end":196},{"cath_id":"3.40.120.10","chopping":"201-426","consensus_level":"medium","plddt":98.0882,"start":201,"end":426},{"cath_id":"3.30.310.50","chopping":"428-567","consensus_level":"high","plddt":94.1891,"start":428,"end":567}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q15124","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q15124-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q15124-F1-predicted_aligned_error_v6.png","plddt_mean":96.38},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=PGM5","jax_strain_url":"https://www.jax.org/strain/search?query=PGM5"},"sequence":{"accession":"Q15124","fasta_url":"https://rest.uniprot.org/uniprotkb/Q15124.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q15124/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q15124"}},"corpus_meta":[{"pmid":"34463962","id":"PMC_34463962","title":"Engineered exosomes for co-delivery of PGM5-AS1 and oxaliplatin to reverse drug resistance in colon cancer.","date":"2021","source":"Journal of cellular physiology","url":"https://pubmed.ncbi.nlm.nih.gov/34463962","citation_count":67,"is_preprint":false},{"pmid":"32412676","id":"PMC_32412676","title":"Long non-coding RNA PGM5-AS1 promotes epithelial-mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR-140-5p-mediated FBN1 inhibition.","date":"2020","source":"Molecular oncology","url":"https://pubmed.ncbi.nlm.nih.gov/32412676","citation_count":34,"is_preprint":false},{"pmid":"8586438","id":"PMC_8586438","title":"A novel human phosphoglucomutase (PGM5) maps to the centromeric region of chromosome 9.","date":"1995","source":"Genomics","url":"https://pubmed.ncbi.nlm.nih.gov/8586438","citation_count":32,"is_preprint":false},{"pmid":"33407592","id":"PMC_33407592","title":"PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer.","date":"2021","source":"Journal of translational medicine","url":"https://pubmed.ncbi.nlm.nih.gov/33407592","citation_count":28,"is_preprint":false},{"pmid":"31185143","id":"PMC_31185143","title":"p53-induced long non-coding RNA PGM5-AS1 inhibits the progression of esophageal squamous cell carcinoma through regulating miR-466/PTEN axis.","date":"2019","source":"IUBMB life","url":"https://pubmed.ncbi.nlm.nih.gov/31185143","citation_count":22,"is_preprint":false},{"pmid":"34212174","id":"PMC_34212174","title":"Circulating lncRNA UCA1 and lncRNA PGM5-AS1 act as potential diagnostic biomarkers for early-stage colorectal cancer.","date":"2021","source":"Bioscience reports","url":"https://pubmed.ncbi.nlm.nih.gov/34212174","citation_count":19,"is_preprint":false},{"pmid":"33986801","id":"PMC_33986801","title":"miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis.","date":"2021","source":"Journal of oncology","url":"https://pubmed.ncbi.nlm.nih.gov/33986801","citation_count":18,"is_preprint":false},{"pmid":"32767323","id":"PMC_32767323","title":"Reduced long noncoding RNA PGM5-AS1 facilitated proliferation and invasion of colorectal cancer through sponging miR-100-5p.","date":"2020","source":"European review for medical and pharmacological sciences","url":"https://pubmed.ncbi.nlm.nih.gov/32767323","citation_count":16,"is_preprint":false},{"pmid":"32354224","id":"PMC_32354224","title":"The Downregulation of lncRNA pgm5-as1 Inhibits the Proliferation and Metastasis Via Increasing miR-484 Expression in Colorectal Cancer.","date":"2020","source":"Cancer biotherapy & radiopharmaceuticals","url":"https://pubmed.ncbi.nlm.nih.gov/32354224","citation_count":15,"is_preprint":false},{"pmid":"35420507","id":"PMC_35420507","title":"Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284.","date":"2022","source":"Bioengineered","url":"https://pubmed.ncbi.nlm.nih.gov/35420507","citation_count":13,"is_preprint":false},{"pmid":"38902704","id":"PMC_38902704","title":"LncRNA PGM5-AS1 inhibits non-small cell lung cancer progression by targeting miRNA-423-5p/SLIT2 axis.","date":"2024","source":"Cancer cell international","url":"https://pubmed.ncbi.nlm.nih.gov/38902704","citation_count":12,"is_preprint":false},{"pmid":"39733221","id":"PMC_39733221","title":"LncRNA PGM5-AS1 Impairs the Resistance of Cervical Cancer to Cisplatin by Regulating the Hippo and PI3K-AKT Pathways.","date":"2024","source":"Biochemical genetics","url":"https://pubmed.ncbi.nlm.nih.gov/39733221","citation_count":8,"is_preprint":false},{"pmid":"36017912","id":"PMC_36017912","title":"LncRNA PGM5-AS1 Inhibits the Progression of Bladder Cancer by Regulating miR-587/SLIT3 Axis.","date":"2022","source":"Critical reviews in eukaryotic gene expression","url":"https://pubmed.ncbi.nlm.nih.gov/36017912","citation_count":7,"is_preprint":false},{"pmid":"38973995","id":"PMC_38973995","title":"PGM5-AS1 Promotes Progression of Diffuse Large B-Cell Lymphoma and Immune Escape by Regulating miR-503-5p.","date":"2024","source":"Journal of inflammation research","url":"https://pubmed.ncbi.nlm.nih.gov/38973995","citation_count":4,"is_preprint":false},{"pmid":"38074999","id":"PMC_38074999","title":"Codonopsis pilosula polysaccharide alleviates rotenone-induced murine brain organoids death through downregulation of gene body DNA methylation modification in the ZIC4/PGM5/CAMTA1 axis.","date":"2023","source":"Biochemistry and biophysics reports","url":"https://pubmed.ncbi.nlm.nih.gov/38074999","citation_count":4,"is_preprint":false},{"pmid":"40035308","id":"PMC_40035308","title":"Tumour protein p53-activated lncRNA PGM5-AS1 suppresses lung cancer growth and stemness by targeting R-spondin1 via microRNA-1247-5p.","date":"2025","source":"Archives of physiology and biochemistry","url":"https://pubmed.ncbi.nlm.nih.gov/40035308","citation_count":2,"is_preprint":false},{"pmid":"41086958","id":"PMC_41086958","title":"PGM5 - AS1 regulates ferroptosis - Mediated gastric cancer cell behavior via ERK signaling pathway.","date":"2025","source":"Cellular signalling","url":"https://pubmed.ncbi.nlm.nih.gov/41086958","citation_count":0,"is_preprint":false},{"pmid":"40298102","id":"PMC_40298102","title":"LncRNA PGM5-AS1 inhibits the progression of breast cancer by inhibiting miR-182-5p.","date":"2025","source":"Nucleosides, nucleotides & nucleic acids","url":"https://pubmed.ncbi.nlm.nih.gov/40298102","citation_count":0,"is_preprint":false},{"pmid":"37471281","id":"PMC_37471281","title":"Retraction statement: Long non-coding RNA PGM5-AS1 promotes epithelial-mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR-140-5p-mediated FBN1 inhibition.","date":"2023","source":"Molecular oncology","url":"https://pubmed.ncbi.nlm.nih.gov/37471281","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":11775,"output_tokens":1180,"usd":0.026513,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7416,"output_tokens":1408,"usd":0.03614,"stage2_stop_reason":"end_turn"},"total_usd":0.062653,"stage1_batch_id":"msgbatch_01CNoDbkChHEkgrJnL7BwtGJ","stage2_batch_id":"msgbatch_016Y4x9E9Wg5VQDANj9quSQD","note":"batch pricing = 50% of standard"},"stage1_raw":"Looking at the corpus, I need to distinguish between:\n1. **PGM5** - the canonical protein-coding gene (phosphoglucomutase 5)\n2. **PGM5-AS1** - an antisense lncRNA from the PGM5 locus (NOT the PGM5 protein)\n\nPapers [1]-[14], [16]-[18] are about PGM5-AS1 (the lncRNA), not the PGM5 protein itself. These are non-protein products of the locus and must be excluded per the rules.\n\nPaper [3] (PMID:8586438) is about the PGM5 protein itself - its identification, mapping, and characterization.\n\nPaper [7] (PMID:33986801) mentions the PGM5 protein directly - it describes PGM5 promoting conversion of glucose-1-phosphate to glucose-6-phosphate and its role in aerobic glycolysis, regulated by miR-1224-3p.\n\nPaper [15] (PMID:38074999) mentions Pgm5 protein expression being regulated by ZIC4 via DNA methylation in mouse brain organoids.\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 1995,\n      \"finding\": \"PGM5 was identified as a novel member of the phosphoglucomutase family, expressed widely in adult and fetal tissues, and mapped to the centromeric region of chromosome 9 by fluorescence in situ hybridization and somatic cell hybrid analysis. RT-PCR and Northern analysis confirmed it is an expressed gene with structural homology to PGM1.\",\n      \"method\": \"RT-PCR, Northern analysis, FISH, somatic cell hybrid mapping\",\n      \"journal\": \"Genomics\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct molecular cloning and chromosomal mapping with two orthogonal localization methods; single lab but multiple methods; no enzymatic activity reconstitution\",\n      \"pmids\": [\"8586438\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"PGM5 protein promotes conversion of glucose-1-phosphate (G1P) to glucose-6-phosphate (G6P) and inhibits breast cancer cell proliferation and migration through regulation of aerobic glycolysis. miR-1224-3p directly targets the 3'-UTR of PGM5 mRNA to suppress PGM5 expression, thereby reducing PGM5-mediated glycolytic function and promoting cancer cell proliferation and migration.\",\n      \"method\": \"Luciferase 3'-UTR reporter assay, glycolysis functional assays, cell proliferation/migration assays with PGM5 knockdown/overexpression\",\n      \"journal\": \"Journal of oncology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — direct 3'-UTR targeting confirmed by luciferase assay and functional rescue experiments in a single lab; enzymatic activity (G1P→G6P) attributed based on family membership rather than direct in vitro reconstitution\",\n      \"pmids\": [\"33986801\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"ZIC4 transcription factor binding at the Pgm5 promoter regulates Pgm5 expression in mouse brain organoids; silencing of Zic4 significantly reduced PGM5 expression, linking ZIC4 upstream of PGM5 in a transcriptional axis. Rotenone-induced gene body hypermethylation of Pgm5 suppressed its expression, which was reversed by Codonopsis pilosula polysaccharide treatment.\",\n      \"method\": \"siRNA silencing of Zic4 with PGM5 protein level measurement, RRBS-Seq for DNA methylation, bioinformatic promoter motif analysis\",\n      \"journal\": \"Biochemistry and biophysics reports\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, single knockdown experiment linking ZIC4 to PGM5 expression; no direct ChIP confirmation of ZIC4 binding; motif analysis is computational\",\n      \"pmids\": [\"38074999\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"PGM5 is a phosphoglucomutase family enzyme that catalyzes conversion of glucose-1-phosphate to glucose-6-phosphate and thereby regulates aerobic glycolysis; it maps to chromosome 9 centromere, is broadly expressed, and its expression is post-transcriptionally suppressed by miR-1224-3p targeting its 3'-UTR and transcriptionally regulated upstream by ZIC4 via gene body DNA methylation.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PGM5 is a phosphoglucomutase-family gene first identified as a widely expressed paralog of PGM1 mapping to the centromeric region of chromosome 9 [#0]. At the functional level, PGM5 promotes conversion of glucose-1-phosphate to glucose-6-phosphate and, through this modulation of aerobic glycolysis, restrains breast cancer cell proliferation and migration; its expression is post-transcriptionally suppressed by miR-1224-3p, which directly targets the PGM5 3'-UTR to relieve this glycolytic brake and thereby promote tumor cell growth and motility [#1]. Beyond these findings and a low-confidence link to ZIC4-dependent transcriptional control, no further mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 1995,\n      \"claim\": \"Established PGM5 as a distinct expressed gene in the phosphoglucomutase family, defining its genomic location and inferred biochemical class before any functional role was known.\",\n      \"evidence\": \"Molecular cloning with RT-PCR/Northern expression analysis and chromosomal mapping by FISH and somatic cell hybrids\",\n      \"pmids\": [\"8586438\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Enzymatic activity was inferred from PGM1 homology, not reconstituted in vitro\",\n        \"No cellular or physiological function assigned\",\n        \"No protein-level characterization\"\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"Connected PGM5 to a metabolic and tumor-suppressive role by linking its glycolytic function to control of cancer cell proliferation/migration and identifying miR-1224-3p as a direct upstream repressor.\",\n      \"evidence\": \"Luciferase 3'-UTR reporter, glycolysis assays, and proliferation/migration assays with PGM5 knockdown and overexpression in breast cancer cells\",\n      \"pmids\": [\"33986801\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"G1P-to-G6P catalysis attributed by family membership, not direct in vitro reconstitution of PGM5 enzyme activity\",\n        \"Single lab; not extended beyond breast cancer context\",\n        \"Mechanistic link between glycolytic flux and proliferation/migration not dissected\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Placed PGM5 downstream of a transcriptional and epigenetic regulatory axis, with ZIC4 and gene-body DNA methylation modulating its expression in a brain organoid model.\",\n      \"evidence\": \"siRNA silencing of Zic4 with PGM5 protein readout, RRBS-Seq methylation profiling, and computational promoter motif analysis in mouse brain organoids\",\n      \"pmids\": [\"38074999\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No direct ChIP confirmation of ZIC4 binding the Pgm5 promoter; binding inferred from computational motif analysis and single knockdown\",\n        \"Functional consequence of PGM5 changes in neural/organoid context not established\",\n        \"Relationship between this transcriptional axis and the metabolic role is unknown\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether PGM5 itself catalyzes the G1P-to-G6P reaction and how its metabolic activity is integrated into broader physiology remain unresolved.\",\n      \"evidence\": \"No direct enzymatic reconstitution or structural study present in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No in vitro demonstration of PGM5 catalytic activity\",\n        \"No structural model or substrate kinetics\",\n        \"Physiological role outside cancer cell lines uncharacterized\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"tie","faith_supported":2,"faith_total":2,"faith_pct":100.0}}