{"gene":"LRCH3","run_date":"2026-06-10T02:59:50","timeline":{"discoveries":[],"current_model":"No mechanistic findings in the available literature."},"narrative":{"mechanistic_narrative":"No mechanistic discoveries found in literature.","teleology":[],"mechanism_profile":null},"prefetch_data":{"uniprot":{"accession":"Q96II8","full_name":"DISP complex protein LRCH3","aliases":["Leucine-rich repeat and calponin homology domain-containing protein 3"],"length_aa":777,"mass_kda":86.1,"function":"As part of the DISP complex, may regulate the association of septins with actin and thereby regulate the actin cytoskeleton","subcellular_location":"Cytoplasm","url":"https://www.uniprot.org/uniprotkb/Q96II8/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/LRCH3","classification":"Not Classified","n_dependent_lines":39,"n_total_lines":1208,"dependency_fraction":0.03228476821192053},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"DOCK7","stoichiometry":10.0},{"gene":"MYO6","stoichiometry":4.0},{"gene":"CALM1","stoichiometry":0.2},{"gene":"CALM2","stoichiometry":0.2},{"gene":"CALM3","stoichiometry":0.2},{"gene":"COPB2","stoichiometry":0.2},{"gene":"HDAC1","stoichiometry":0.2},{"gene":"MIF","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/LRCH3","total_profiled":1310},"omim":[],"hpa":{"profiled":true,"resolved_as":"","reliability":"Enhanced","locations":[{"location":"Cytosol","reliability":"Enhanced"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/LRCH3"},"hgnc":{"alias_symbol":["MGC4126"],"prev_symbol":[]},"alphafold":{"accession":"Q96II8","domains":[{"cath_id":"3.80.10.10","chopping":"53-279","consensus_level":"medium","plddt":94.0464,"start":53,"end":279},{"cath_id":"1.10.418.10","chopping":"653-765","consensus_level":"high","plddt":88.6141,"start":653,"end":765}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q96II8","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q96II8-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q96II8-F1-predicted_aligned_error_v6.png","plddt_mean":65.44},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=LRCH3","jax_strain_url":"https://www.jax.org/strain/search?query=LRCH3"},"sequence":{"accession":"Q96II8","fasta_url":"https://rest.uniprot.org/uniprotkb/Q96II8.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q96II8/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q96II8"}},"corpus_meta":[{"pmid":"32123303","id":"PMC_32123303","title":"Melanomas with activating RAF1 fusions: clinical, histopathologic, and molecular profiles.","date":"2020","source":"Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc","url":"https://pubmed.ncbi.nlm.nih.gov/32123303","citation_count":35,"is_preprint":false},{"pmid":"35871080","id":"PMC_35871080","title":"Identification of fusions with potential clinical significance in melanoma.","date":"2022","source":"Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc","url":"https://pubmed.ncbi.nlm.nih.gov/35871080","citation_count":30,"is_preprint":false},{"pmid":"30852025","id":"PMC_30852025","title":"Candidate genes associated with the heritable humoral response to Mycobacterium avium ssp. paratuberculosis in dairy cows have factors in common with gastrointestinal diseases in humans.","date":"2019","source":"Journal of dairy science","url":"https://pubmed.ncbi.nlm.nih.gov/30852025","citation_count":27,"is_preprint":false},{"pmid":"19799599","id":"PMC_19799599","title":"Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs.","date":"2009","source":"Animal genetics","url":"https://pubmed.ncbi.nlm.nih.gov/19799599","citation_count":24,"is_preprint":false},{"pmid":"38570000","id":"PMC_38570000","title":"Super enhancer-associated circRNA-circLrch3 regulates hypoxia-induced pulmonary arterial smooth muscle cells pyroptosis by formation of R-loop with host gene.","date":"2024","source":"International journal of biological macromolecules","url":"https://pubmed.ncbi.nlm.nih.gov/38570000","citation_count":14,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":5642,"output_tokens":346,"usd":0.011058,"stage1_stop_reason":"end_turn"},"stage2":{"model":"","input_tokens":0,"output_tokens":0,"usd":0.0},"total_usd":0.011058,"stage1_batch_id":"msgbatch_01BbTCMyrRPyhtEq6b1MdwCN","stage2_batch_id":"msgbatch_011dEed5bbxYvTAaQWKJevxu","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [],\n  \"current_model\": \"No mechanistic findings in the available literature.\"\n}\n```\n\n**Reasoning:** After reviewing all five papers:\n\n1. **PMID:32123303** — LRCH3 appears only as a fusion partner in RAF1 fusion melanomas. No mechanistic data about LRCH3 protein function is established.\n2. **PMID:35871080** — Again, LRCH3 is listed as a fusion partner (LRCH3::RAF1) in melanoma. No mechanistic experiment about LRCH3 protein is described.\n3. **PMID:30852025** — LRCH3 is listed as a candidate gene from a GWAS-type association study in dairy cattle. No mechanistic experiment is described (excluded per rules: GWAS/association).\n4. **PMID:19799599** — LRCH3 is mentioned as a gene where polymorphisms were identified in a haplotype mapping study in pigs. No functional/mechanistic experiment is described.\n5. **PMID:38570000** — This paper is about **circLrch3**, a circular RNA product of the Lrch3 locus — explicitly a non-protein-coding RNA product. Per the rules, non-protein products of the same locus must be excluded.\n\nNo paper in the corpus describes a direct experiment establishing how the canonical LRCH3 protein works mechanistically.","stage2_raw":"","audit_flag":null,"evaluation":null}