{"gene":"KIF1B","run_date":"2026-06-10T02:59:49","timeline":{"discoveries":[{"year":1994,"finding":"KIF1B is a monomeric, microtubule plus end-directed motor protein that colocalizes with mitochondria in vivo, is concentrated in the mitochondrial subcellular fraction, and can transport mitochondria along microtubules in vitro.","method":"Rotary shadowing electron microscopy (monomeric structure), immunocytochemistry (colocalization with mitochondria), subcellular fractionation, in vitro microtubule-based transport assay","journal":"Cell","confidence":"High","confidence_rationale":"Tier 1 / Strong — in vitro reconstitution of mitochondrial transport, direct subcellular fractionation, and electron microscopy in a foundational study; independently cited and replicated concept","pmids":["7528108"],"is_preprint":false},{"year":1999,"finding":"The Kif1b gene generates at least two major kinesin isoforms by alternative splicing: a shorter KIF1Bα (130 kDa) and a longer KIF1Bβ (204 kDa) with a novel C-terminal cargo-binding domain homologous to KIF1A, suggesting distinct cargo specificities.","method":"cDNA cloning, Northern blot analysis, haplotype mapping, alternative exon identification","journal":"Gene","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — molecular cloning with Northern blot across multiple tissues; functional cargo difference is inferred from sequence homology, not directly tested","pmids":["10571041"],"is_preprint":false},{"year":1999,"finding":"The major brain isoform of Kif1b lacks the putative mitochondria-binding domain present in the originally described isoform, indicating that alternative splicing produces an isoform likely to have different cargo-binding specificity.","method":"cDNA library screening, Northern blot analysis, sequence comparison of C-terminal domains","journal":"Mammalian genome","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — molecular cloning and Northern blot; cargo-binding difference inferred from domain analysis, not functionally validated in transport assay","pmids":["10341097"],"is_preprint":false},{"year":2009,"finding":"Alternative splicing of KIF1B produces variants that differ in insertion sequences within the K-loop and hinge regions; variants containing both insertions show higher microtubule-dependent ATPase activity and microtubule affinity than those without insertions, as demonstrated by in vitro ATPase assays and mutational analysis of the K-loop.","method":"Recombinant protein expression, microtubule-dependent ATPase assay, in vitro motility assay, K-loop insertion mutagenesis","journal":"Traffic","confidence":"High","confidence_rationale":"Tier 1 / Moderate — in vitro ATPase reconstitution with mutagenesis of the K-loop in a single rigorous study; multiple orthogonal methods","pmids":["19744141"],"is_preprint":false},{"year":2009,"finding":"Kif1b is required for localization of myelin basic protein (mbp) mRNA to processes of myelinating oligodendrocytes in zebrafish; loss of kif1b causes ectopic appearance of myelin-like membrane and mislocalization of myelin proteins to oligodendrocyte cell bodies.","method":"Zebrafish kif1b mutant analysis, in vivo imaging, mRNA localization assays","journal":"Nature genetics","confidence":"High","confidence_rationale":"Tier 2 / Moderate — genetic loss-of-function in zebrafish with defined cellular phenotype (mRNA mislocalization and ectopic myelin), multiple readouts","pmids":["19503091"],"is_preprint":false},{"year":2009,"finding":"Kif1b is required for outgrowth of some of the longest axons in both peripheral and central nervous systems in zebrafish, establishing a role for this motor in axon elongation in vivo.","method":"Zebrafish kif1b mutant analysis, in vivo imaging of axon growth","journal":"Nature genetics","confidence":"High","confidence_rationale":"Tier 2 / Moderate — genetic loss-of-function in zebrafish with defined axon-length phenotype, multiple neuron types examined","pmids":["19503091"],"is_preprint":false},{"year":2013,"finding":"Leptin promotes gastric cancer cell invasion partly through KIF1B-dependent surface localization of MT1-MMP; co-immunoprecipitation showed leptin enhances the KIF1B–MT1-MMP interaction in a time-dependent manner, and siRNA knockdown of KIF1B inhibits leptin-induced membrane targeting of MT1-MMP.","method":"Co-immunoprecipitation, siRNA knockdown, cell surface biotinylation assay, flow cytometry, transwell invasion assay","journal":"Carcinogenesis","confidence":"Medium","confidence_rationale":"Tier 2–3 / Moderate — reciprocal interaction by Co-IP plus functional siRNA knockdown with defined phenotype; single lab","pmids":["23354307"],"is_preprint":false},{"year":2015,"finding":"KIF1B promotes glioma cell migration and invasion through cell surface localization of MT1-MMP; siRNA knockdown of KIF1B reduces membranous MT1-MMP levels without altering total cellular MT1-MMP, consistent with a transport function.","method":"siRNA knockdown, transwell migration/invasion assay, cell surface MT1-MMP measurement","journal":"Oncology reports","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — siRNA knockdown with defined phenotype, corroborates gastric cancer finding; single lab, single primary method","pmids":["26576027"],"is_preprint":false},{"year":2016,"finding":"The Kif1B motor and its binding partner KBP are required for anterograde transport of SCG10 (Stathmin-2) to axon growth cones in zebrafish; loss of KBP reduces SCG10 at growth cones, alters microtubule stability, and truncates axons—phenotypes rescued by SCG10 overexpression. Loss of Kif1B or KBP did not inhibit mitochondrial transport in this in vivo context.","method":"Zebrafish mutant analysis (kbp mutants), in vivo live imaging of SCG10 and microtubule dynamics, genetic epistasis (SCG10 overexpression rescue), mitochondrial transport imaging","journal":"The Journal of neuroscience","confidence":"High","confidence_rationale":"Tier 2 / Strong — multiple orthogonal methods (live imaging, genetic rescue, microtubule stability assay), negative result for mitochondrial transport explicitly reported, replicated in zebrafish in vivo","pmids":["27358458"],"is_preprint":false},{"year":2016,"finding":"KIF1B loss-of-function in mouse oocytes causes abnormal polar body extrusion, disordered spindle dynamics, chromosome congression defects, increased aneuploidy, and impaired embryonic development; KIF1B depletion also disrupts mitochondrial distribution and reduces ATP abundance in oocytes.","method":"siRNA-mediated KIF1B depletion, immunofluorescence for spindle assembly and chromosome alignment, mitochondrial distribution assay, ATP quantification","journal":"Molecular reproduction and development","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — loss-of-function with defined cellular phenotypes (spindle, chromosome, mitochondria), multiple readouts; single lab","pmids":["27696585"],"is_preprint":false},{"year":2025,"finding":"KIF1B promotes NLRP3 inflammasome-mediated pyroptosis in asthma; KIF1B knockdown reduces expression of NLRP3, cleaved caspase-1, and cleaved GSDMD, and NLRP3 overexpression abolishes these protective effects, placing KIF1B upstream of NLRP3 in airway inflammatory signaling.","method":"siRNA knockdown, NLRP3 overexpression rescue, in vitro IL-13-stimulated airway epithelial cells, OVA-induced mouse asthma model, Western blot for pyroptosis markers","journal":"Journal of cellular and molecular medicine","confidence":"Medium","confidence_rationale":"Tier 2–3 / Moderate — genetic epistasis (NLRP3 overexpression reversal), in vivo and in vitro models; single lab","pmids":["41384344"],"is_preprint":false},{"year":2024,"finding":"TRIM14 directly interacts with KIF1B (confirmed by co-immunoprecipitation and immunofluorescence co-localization) and positively regulates KIF1B expression; this TRIM14–KIF1B axis drives renal tubular injury in diabetic nephropathy via TLR4/NF-κB pathway activation.","method":"Co-immunoprecipitation, immunofluorescence, siRNA knockdown of TRIM14, TRIM14 overexpression, Western blot, in vivo rat DN model, protein-protein interaction network analysis","journal":"Diabetes, metabolic syndrome and obesity","confidence":"Medium","confidence_rationale":"Tier 2–3 / Moderate — Co-IP plus functional knockdown with pathway readout; single lab, in vivo and in vitro corroboration","pmids":["41710718"],"is_preprint":false}],"current_model":"KIF1B is a monomeric, microtubule plus-end-directed kinesin-3 motor that exists as multiple alternatively spliced isoforms with distinct cargo specificities: the originally described KIF1Bα isoform transports mitochondria along axonal microtubules, while the predominant KIF1Bβ isoform (lacking the mitochondria-binding domain) transports synaptic vesicle precursors and, together with its binding partner KBP, mediates anterograde transport of the microtubule regulator SCG10 to axon growth cones to control microtubule dynamics and axon elongation; KIF1B also localizes MT1-MMP to the cell surface in a transport-dependent manner promoting tumor invasion, is required in oligodendrocytes for mRNA localization of myelin basic protein to cellular processes, regulates spindle assembly and mitochondrial distribution during oocyte meiosis, and in the context of inflammatory signaling acts upstream of the NLRP3 inflammasome; the K-loop insertion in alternatively spliced variants modulates microtubule ATPase activity and affinity."},"narrative":{"mechanistic_narrative":"KIF1B is a monomeric, microtubule plus-end-directed kinesin-3 motor that drives anterograde axonal transport and is required in vivo for the outgrowth of the longest peripheral and central axons [PMID:7528108, PMID:19503091]. The originally characterized isoform colocalizes with and transports mitochondria along microtubules in vitro [PMID:7528108], while alternative splicing of the gene generates distinct isoforms—including a longer brain-enriched variant bearing a novel KIF1A-homologous C-terminal cargo-binding domain and a major isoform lacking the mitochondria-binding domain—that confer distinct cargo specificities [PMID:10571041, PMID:10341097]. Motor output is tuned at the mechanochemical level: splice variants differing in K-loop and hinge insertions display higher microtubule-dependent ATPase activity and microtubule affinity, with the K-loop directly modulating these properties [PMID:19744141]. As a transport motor, KIF1B together with its binding partner KBP delivers the microtubule regulator SCG10/Stathmin-2 to axon growth cones to control microtubule stability and axon elongation, a function genetically separable from mitochondrial transport in vivo [PMID:27358458], and it is also required for localization of myelin basic protein mRNA to oligodendrocyte processes [PMID:19503091]. Beyond neural transport, KIF1B mediates cell-surface delivery of the matrix metalloproteinase MT1-MMP to promote tumor cell migration and invasion [PMID:23354307, PMID:26576027], regulates spindle dynamics, chromosome congression, and mitochondrial distribution during oocyte meiosis [PMID:27696585], and acts upstream of NLRP3 inflammasome–driven pyroptosis in airway inflammation [PMID:41384344].","teleology":[{"year":1994,"claim":"Established KIF1B as a bona fide motor protein with intrinsic cargo-transport capacity, answering whether it could physically move organelles along microtubules.","evidence":"Rotary-shadowing EM, subcellular fractionation, and in vitro microtubule-based transport reconstitution showing monomeric KIF1B transporting mitochondria","pmids":["7528108"],"confidence":"High","gaps":["Mechanism of cargo (mitochondria) attachment not defined","Regulation of motor activity in cells not addressed","Did not anticipate isoform diversity"]},{"year":1999,"claim":"Revealed that the Kif1b locus produces multiple isoforms by alternative splicing with divergent C-terminal cargo-binding domains, reframing KIF1B as a family of motors with potentially distinct cargoes rather than a single mitochondrial transporter.","evidence":"cDNA cloning, Northern blot across tissues, and C-terminal domain sequence comparison identifying KIF1Bα, KIF1Bβ, and a brain isoform lacking the mitochondria-binding domain","pmids":["10571041","10341097"],"confidence":"Medium","gaps":["Distinct cargo specificities inferred from sequence, not tested in transport assays","Tissue-specific functions of each isoform unresolved"]},{"year":2009,"claim":"Connected splice-variant sequence differences to motor mechanochemistry, showing the K-loop directly tunes microtubule binding and ATPase output.","evidence":"Recombinant protein microtubule-dependent ATPase and motility assays with K-loop insertion mutagenesis","pmids":["19744141"],"confidence":"High","gaps":["Physiological consequence of differential ATPase tuning in vivo not established","Does not link mechanochemical state to specific cargo selection"]},{"year":2009,"claim":"Defined an in vivo neural requirement for KIF1B in long-axon outgrowth and in mRNA localization for myelination, extending its role beyond organelle transport to cargo delivery underpinning neural development.","evidence":"Zebrafish kif1b loss-of-function mutants with in vivo imaging of axon growth and mbp mRNA localization","pmids":["19503091"],"confidence":"High","gaps":["Molecular cargo delivered to support axon outgrowth not yet identified at this stage","Mechanism of mRNA cargo coupling unknown"]},{"year":2016,"claim":"Identified SCG10/Stathmin-2 as a functional KIF1B cargo delivered via the partner KBP, mechanistically explaining the axon-outgrowth phenotype and dissociating it from mitochondrial transport.","evidence":"Zebrafish kbp mutants, live imaging of SCG10 and microtubule dynamics, SCG10-overexpression rescue, and explicit mitochondrial transport controls","pmids":["27358458"],"confidence":"High","gaps":["Direct biochemical KIF1B–KBP–SCG10 cargo complex not reconstituted","Relationship between KBP-dependent and mitochondrial transport functions not fully reconciled"]},{"year":2016,"claim":"Extended KIF1B function to cell division, showing it governs meiotic spindle dynamics, chromosome congression, and mitochondrial distribution in oocytes.","evidence":"siRNA depletion in mouse oocytes with immunofluorescence of spindle/chromosomes, mitochondrial distribution, and ATP quantification","pmids":["27696585"],"confidence":"Medium","gaps":["Direct molecular targets at the spindle not identified","Single lab; isoform responsible not specified","Whether spindle defect is secondary to mitochondrial/ATP disruption unclear"]},{"year":2013,"claim":"Implicated KIF1B in cancer cell invasion through transport-dependent surface delivery of MT1-MMP, generalizing its transport role to disease-relevant cargo.","evidence":"Co-IP, siRNA knockdown, cell-surface biotinylation, and transwell invasion assays in leptin-stimulated gastric cancer cells","pmids":["23354307"],"confidence":"Medium","gaps":["Direct vs indirect KIF1B–MT1-MMP interaction not fully resolved","Single lab at time of report"]},{"year":2015,"claim":"Corroborated KIF1B-dependent MT1-MMP surface localization as a pro-invasive mechanism in a second tumor type, strengthening the transport interpretation.","evidence":"siRNA knockdown with surface vs total MT1-MMP measurement and migration/invasion assays in glioma cells","pmids":["26576027"],"confidence":"Medium","gaps":["Single primary method (knockdown)","Mechanism of MT1-MMP cargo coupling unresolved"]},{"year":2024,"claim":"Positioned KIF1B downstream of TRIM14 in an inflammatory signaling axis driving renal tubular injury, indicating regulated KIF1B expression in disease.","evidence":"Co-IP, immunofluorescence co-localization, TRIM14 knockdown/overexpression with TLR4/NF-κB readouts in a rat diabetic nephropathy model","pmids":["41710718"],"confidence":"Medium","gaps":["Whether KIF1B motor/transport activity is required for the phenotype not tested","Single lab"]},{"year":2025,"claim":"Placed KIF1B upstream of NLRP3 inflammasome activation and pyroptosis in airway inflammation, expanding its role into innate immune signaling.","evidence":"siRNA knockdown with NLRP3-overexpression rescue and pyroptosis marker Western blots in IL-13-stimulated epithelial cells and an OVA mouse asthma model","pmids":["41384344"],"confidence":"Medium","gaps":["Mechanistic link between a microtubule motor and NLRP3 activation undefined","Single lab; direct interactors in this pathway not identified"]},{"year":null,"claim":"How distinct KIF1B splice isoforms select and couple their respective cargoes (mitochondria, synaptic vesicle precursors, SCG10/KBP, MT1-MMP, mRNAs) at the molecular level, and how the motor connects to inflammasome and spindle functions, remain unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No reconstituted isoform-specific cargo-adaptor complexes","Mechanistic basis for non-transport roles (NLRP3, spindle) unestablished","Isoform-resolved functional assignment across tissues incomplete"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0003774","term_label":"cytoskeletal motor activity","supporting_discovery_ids":[0,3]},{"term_id":"GO:0140657","term_label":"ATP-dependent activity","supporting_discovery_ids":[0,3]},{"term_id":"GO:0008092","term_label":"cytoskeletal protein binding","supporting_discovery_ids":[0,3]}],"localization":[{"term_id":"GO:0005856","term_label":"cytoskeleton","supporting_discovery_ids":[0]},{"term_id":"GO:0005739","term_label":"mitochondrion","supporting_discovery_ids":[0]},{"term_id":"GO:0005886","term_label":"plasma membrane","supporting_discovery_ids":[6,7]}],"pathway":[{"term_id":"R-HSA-9609507","term_label":"Protein localization","supporting_discovery_ids":[0,8]},{"term_id":"R-HSA-5653656","term_label":"Vesicle-mediated transport","supporting_discovery_ids":[0,8]}],"complexes":[],"partners":["KBP","SCG10","MT1-MMP","TRIM14"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"O60333","full_name":"Kinesin-like protein KIF1B","aliases":[],"length_aa":1816,"mass_kda":204.5,"function":"Has a plus-end-directed microtubule motor activity and functions as a motor for transport of vesicles and organelles along microtubules Has a plus-end-directed microtubule motor activity and functions as a motor for anterograde synaptic vesicle transport along axonal microtubules from the cell body to the presynapse in neuronal cells (By similarity). Functions as a downstream effector in a developmental apoptotic pathway that is activated when nerve growth factor (NGF) becomes limiting for neuronal progenitor cells (PubMed:18334619) Has a plus-end-directed microtubule motor activity and functions as a motor for anterograde transport of mitochondria","subcellular_location":"Mitochondrion","url":"https://www.uniprot.org/uniprotkb/O60333/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/KIF1B","classification":"Not Classified","n_dependent_lines":17,"n_total_lines":1208,"dependency_fraction":0.014072847682119206},"opencell":{"profiled":true,"resolved_as":"","ensg_id":"ENSG00000054523","cell_line_id":"CID001415","localizations":[{"compartment":"cytoplasmic","grade":3},{"compartment":"big_aggregates","grade":2},{"compartment":"nucleoplasm","grade":2},{"compartment":"centrosome","grade":1}],"interactors":[{"gene":"CALM1","stoichiometry":0.2},{"gene":"CALM2","stoichiometry":0.2},{"gene":"CALM3","stoichiometry":0.2},{"gene":"CSNK2A1","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/target/CID001415","total_profiled":1310},"omim":[{"mim_id":"612596","title":"MULTIPLE SCLEROSIS, SUSCEPTIBILITY TO, 4; MS4","url":"https://www.omim.org/entry/612596"},{"mim_id":"609460","title":"GOLDBERG-SHPRINTZEN SYNDROME; GOSHS","url":"https://www.omim.org/entry/609460"},{"mim_id":"609367","title":"KINESIN FAMILY BINDING PROTEIN; KIFBP","url":"https://www.omim.org/entry/609367"},{"mim_id":"609260","title":"CHARCOT-MARIE-TOOTH DISEASE, AXONAL, AUTOSOMAL DOMINANT, TYPE 2A2A; CMT2A2A","url":"https://www.omim.org/entry/609260"},{"mim_id":"608507","title":"MITOFUSIN 2; MFN2","url":"https://www.omim.org/entry/608507"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Uncertain","locations":[{"location":"Microtubules","reliability":"Uncertain"},{"location":"Mid piece","reliability":"Uncertain"},{"location":"Principal piece","reliability":"Uncertain"},{"location":"End piece","reliability":"Uncertain"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in all","driving_tissues":[{"tissue":"retina","ntpm":118.5},{"tissue":"skeletal muscle","ntpm":184.8},{"tissue":"tongue","ntpm":141.5}],"url":"https://www.proteinatlas.org/search/KIF1B"},"hgnc":{"alias_symbol":["KIAA0591","KLP","HMSNII"],"prev_symbol":["CMT2A","CMT2"]},"alphafold":{"accession":"O60333","domains":[{"cath_id":"3.40.850.10","chopping":"5-293_302-363","consensus_level":"medium","plddt":83.4187,"start":5,"end":363},{"cath_id":"-","chopping":"499-527","consensus_level":"medium","plddt":57.8093,"start":499,"end":527},{"cath_id":"2.60.200.20","chopping":"529-636","consensus_level":"medium","plddt":82.1722,"start":529,"end":636},{"cath_id":"-","chopping":"760-804_823-857","consensus_level":"medium","plddt":82.4984,"start":760,"end":857},{"cath_id":"2.60.40,2.60.210","chopping":"969-1017_1026-1044_1111-1202","consensus_level":"medium","plddt":78.7741,"start":969,"end":1202},{"cath_id":"2.60.40","chopping":"1244-1417_1436-1450_1691-1698","consensus_level":"high","plddt":83.0887,"start":1244,"end":1698},{"cath_id":"2.30.29.30","chopping":"1707-1809","consensus_level":"high","plddt":78.7963,"start":1707,"end":1809}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/O60333","model_url":"https://alphafold.ebi.ac.uk/files/AF-O60333-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-O60333-F1-predicted_aligned_error_v6.png","plddt_mean":65.94},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=KIF1B","jax_strain_url":"https://www.jax.org/strain/search?query=KIF1B"},"sequence":{"accession":"O60333","fasta_url":"https://rest.uniprot.org/uniprotkb/O60333.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/O60333/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/O60333"}},"corpus_meta":[{"pmid":"7528108","id":"PMC_7528108","title":"KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria.","date":"1994","source":"Cell","url":"https://pubmed.ncbi.nlm.nih.gov/7528108","citation_count":496,"is_preprint":false},{"pmid":"17296794","id":"PMC_17296794","title":"Complementation between mouse Mfn1 and Mfn2 protects mitochondrial fusion defects caused by CMT2A disease mutations.","date":"2007","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/17296794","citation_count":285,"is_preprint":false},{"pmid":"21508331","id":"PMC_21508331","title":"MFN2 mutations cause severe phenotypes in most patients with CMT2A.","date":"2011","source":"Neurology","url":"https://pubmed.ncbi.nlm.nih.gov/21508331","citation_count":177,"is_preprint":false},{"pmid":"31664033","id":"PMC_31664033","title":"Structural insights of human mitofusin-2 into mitochondrial fusion and CMT2A onset.","date":"2019","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/31664033","citation_count":144,"is_preprint":false},{"pmid":"16043786","id":"PMC_16043786","title":"Clinical and electrophysiologic features of CMT2A with mutations in the mitofusin 2 gene.","date":"2005","source":"Neurology","url":"https://pubmed.ncbi.nlm.nih.gov/16043786","citation_count":139,"is_preprint":false},{"pmid":"8406488","id":"PMC_8406488","title":"Localization of a gene (CMT2A) for autosomal dominant Charcot-Marie-Tooth disease type 2 to chromosome 1p and evidence of genetic heterogeneity.","date":"1993","source":"Genomics","url":"https://pubmed.ncbi.nlm.nih.gov/8406488","citation_count":139,"is_preprint":false},{"pmid":"18997785","id":"PMC_18997785","title":"Genetic variation in the KIF1B locus influences susceptibility to multiple sclerosis.","date":"2008","source":"Nature genetics","url":"https://pubmed.ncbi.nlm.nih.gov/18997785","citation_count":136,"is_preprint":false},{"pmid":"19503091","id":"PMC_19503091","title":"Kif1b is essential for mRNA localization in oligodendrocytes and development of myelinated axons.","date":"2009","source":"Nature genetics","url":"https://pubmed.ncbi.nlm.nih.gov/19503091","citation_count":134,"is_preprint":false},{"pmid":"18726616","id":"PMC_18726616","title":"A germline mutation of the KIF1B beta gene on 1p36 in a family with neural and nonneural tumors.","date":"2008","source":"Human genetics","url":"https://pubmed.ncbi.nlm.nih.gov/18726616","citation_count":123,"is_preprint":false},{"pmid":"15753033","id":"PMC_15753033","title":"The KLP-6 kinesin is required for male mating behaviors and polycystin localization in Caenorhabditis elegans.","date":"2005","source":"Current biology : CB","url":"https://pubmed.ncbi.nlm.nih.gov/15753033","citation_count":105,"is_preprint":false},{"pmid":"14517316","id":"PMC_14517316","title":"Localization of Pavarotti-KLP in living Drosophila embryos suggests roles in reorganizing the cortical cytoskeleton during the mitotic cycle.","date":"2003","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/14517316","citation_count":68,"is_preprint":false},{"pmid":"29898954","id":"PMC_29898954","title":"Mitofusin gain and loss of function drive pathogenesis in Drosophila models of CMT2A neuropathy.","date":"2018","source":"EMBO reports","url":"https://pubmed.ncbi.nlm.nih.gov/29898954","citation_count":67,"is_preprint":false},{"pmid":"21757353","id":"PMC_21757353","title":"Kinesin-3 KLP-6 regulates intraflagellar transport in male-specific cilia of Caenorhabditis elegans.","date":"2011","source":"Current biology : CB","url":"https://pubmed.ncbi.nlm.nih.gov/21757353","citation_count":59,"is_preprint":false},{"pmid":"12937278","id":"PMC_12937278","title":"KLP-18, a Klp2 kinesin, is required for assembly of acentrosomal meiotic spindles in Caenorhabditis elegans.","date":"2003","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/12937278","citation_count":54,"is_preprint":false},{"pmid":"32147437","id":"PMC_32147437","title":"HDAC6 inhibition promotes α-tubulin acetylation and ameliorates CMT2A peripheral neuropathy in mice.","date":"2020","source":"Experimental neurology","url":"https://pubmed.ncbi.nlm.nih.gov/32147437","citation_count":53,"is_preprint":false},{"pmid":"15452142","id":"PMC_15452142","title":"Loss of KLP-19 polar ejection force causes misorientation and missegregation of holocentric chromosomes.","date":"2004","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/15452142","citation_count":53,"is_preprint":false},{"pmid":"33074106","id":"PMC_33074106","title":"Burst mitofusin activation reverses neuromuscular dysfunction in murine CMT2A.","date":"2020","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/33074106","citation_count":47,"is_preprint":false},{"pmid":"27559133","id":"PMC_27559133","title":"Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1.","date":"2016","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/27559133","citation_count":46,"is_preprint":false},{"pmid":"23354307","id":"PMC_23354307","title":"Leptin-mediated regulation of MT1-MMP localization is KIF1B dependent and enhances gastric cancer cell invasion.","date":"2013","source":"Carcinogenesis","url":"https://pubmed.ncbi.nlm.nih.gov/23354307","citation_count":43,"is_preprint":false},{"pmid":"26370499","id":"PMC_26370499","title":"KLP-7 acts through the Ndc80 complex to limit pole number in C. elegans oocyte meiotic spindle assembly.","date":"2015","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/26370499","citation_count":42,"is_preprint":false},{"pmid":"28289130","id":"PMC_28289130","title":"Inhibition of ectopic microtubule assembly by the kinesin-13 KLP-7 prevents chromosome segregation and cytokinesis defects in oocytes.","date":"2017","source":"Development (Cambridge, England)","url":"https://pubmed.ncbi.nlm.nih.gov/28289130","citation_count":41,"is_preprint":false},{"pmid":"9409358","id":"PMC_9409358","title":"Linkage mapping of the gene for Charcot-Marie-Tooth disease type 2 to chromosome 1p (CMT2A) and the clinical features of CMT2A.","date":"1997","source":"Neurology","url":"https://pubmed.ncbi.nlm.nih.gov/9409358","citation_count":38,"is_preprint":false},{"pmid":"19812251","id":"PMC_19812251","title":"A mutation associated with CMT2A neuropathy causes defects in Fzo1 GTP hydrolysis, ubiquitylation, and protein turnover.","date":"2009","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/19812251","citation_count":34,"is_preprint":false},{"pmid":"9079818","id":"PMC_9079818","title":"Pax-5 is identical to EBB-1/KLP and binds to the VpreB and lambda5 promoters as well as the KI and KII sites upstream of the Jkappa genes.","date":"1997","source":"European journal of immunology","url":"https://pubmed.ncbi.nlm.nih.gov/9079818","citation_count":32,"is_preprint":false},{"pmid":"23803045","id":"PMC_23803045","title":"The KIF1B (rs17401966) single nucleotide polymorphism is not associated with the development of HBV-related hepatocellular carcinoma in Thai patients.","date":"2013","source":"Asian Pacific journal of cancer prevention : APJCP","url":"https://pubmed.ncbi.nlm.nih.gov/23803045","citation_count":32,"is_preprint":false},{"pmid":"26168236","id":"PMC_26168236","title":"The KLP-7 Residue S546 Is a Putative Aurora Kinase Site Required for Microtubule Regulation at the Centrosome in C. elegans.","date":"2015","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/26168236","citation_count":31,"is_preprint":false},{"pmid":"9613448","id":"PMC_9613448","title":"Immunostimulatory effects of dimerized lysozyme (KLP-602) on the nonspecific defense mechanisms and protection against furunculosis in salmonids.","date":"1998","source":"Veterinary immunology and immunopathology","url":"https://pubmed.ncbi.nlm.nih.gov/9613448","citation_count":31,"is_preprint":false},{"pmid":"26576027","id":"PMC_26576027","title":"KIF1B promotes glioma migration and invasion via cell surface localization of MT1-MMP.","date":"2015","source":"Oncology reports","url":"https://pubmed.ncbi.nlm.nih.gov/26576027","citation_count":30,"is_preprint":false},{"pmid":"10571041","id":"PMC_10571041","title":"A novel mouse kinesin of the UNC-104/KIF1 subfamily encoded by the Kif1b gene.","date":"1999","source":"Gene","url":"https://pubmed.ncbi.nlm.nih.gov/10571041","citation_count":30,"is_preprint":false},{"pmid":"11526494","id":"PMC_11526494","title":"Genomic structure and mutational analysis of the human KIF1B gene which is homozygously deleted in neuroblastoma at chromosome 1p36.2.","date":"2001","source":"Oncogene","url":"https://pubmed.ncbi.nlm.nih.gov/11526494","citation_count":29,"is_preprint":false},{"pmid":"22855524","id":"PMC_22855524","title":"The kinesin-3 family motor KLP-4 regulates anterograde trafficking of GLR-1 glutamate receptors in the ventral nerve cord of Caenorhabditis elegans.","date":"2012","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/22855524","citation_count":29,"is_preprint":false},{"pmid":"8613797","id":"PMC_8613797","title":"mRNA expression of KIF1A, KIF1B, KIF2, KIF3A, KIF3B, KIF4, KIF5, and cytoplasmic dynein during axonal regeneration.","date":"1996","source":"The Journal of neuroscience : the official journal of the Society for Neuroscience","url":"https://pubmed.ncbi.nlm.nih.gov/8613797","citation_count":28,"is_preprint":false},{"pmid":"10341097","id":"PMC_10341097","title":"The major brain isoform of kif1b lacks the putative mitochondria-binding domain.","date":"1999","source":"Mammalian genome : official journal of the International Mammalian Genome Society","url":"https://pubmed.ncbi.nlm.nih.gov/10341097","citation_count":27,"is_preprint":false},{"pmid":"17437620","id":"PMC_17437620","title":"Mitofusin 2 gene mutation (R94Q) causing severe early-onset axonal polyneuropathy (CMT2A).","date":"2007","source":"European journal of neurology","url":"https://pubmed.ncbi.nlm.nih.gov/17437620","citation_count":26,"is_preprint":false},{"pmid":"22363396","id":"PMC_22363396","title":"HBV-related hepatocellular carcinoma susceptibility gene KIF1B is not associated with development of chronic hepatitis B.","date":"2012","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/22363396","citation_count":25,"is_preprint":false},{"pmid":"27358458","id":"PMC_27358458","title":"Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones.","date":"2016","source":"The Journal of neuroscience : the official journal of the Society for Neuroscience","url":"https://pubmed.ncbi.nlm.nih.gov/27358458","citation_count":25,"is_preprint":false},{"pmid":"24072599","id":"PMC_24072599","title":"Homozygous truncating mutation of the KBP gene, encoding a KIF1B-binding protein, in a familial case of fetal polymicrogyria.","date":"2013","source":"Neurogenetics","url":"https://pubmed.ncbi.nlm.nih.gov/24072599","citation_count":24,"is_preprint":false},{"pmid":"34870686","id":"PMC_34870686","title":"MITOL-mediated DRP1 ubiquitylation and degradation promotes mitochondrial hyperfusion in a CMT2A-linked MFN2 mutant.","date":"2022","source":"Journal of cell science","url":"https://pubmed.ncbi.nlm.nih.gov/34870686","citation_count":23,"is_preprint":false},{"pmid":"23028799","id":"PMC_23028799","title":"Role of single nucleotide polymorphisms of KIF1B gene in HBV-associated viral hepatitis.","date":"2012","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/23028799","citation_count":21,"is_preprint":false},{"pmid":"30830587","id":"PMC_30830587","title":"Disease Modeling and Therapeutic Strategies in CMT2A: State of the Art.","date":"2019","source":"Molecular neurobiology","url":"https://pubmed.ncbi.nlm.nih.gov/30830587","citation_count":20,"is_preprint":false},{"pmid":"23634229","id":"PMC_23634229","title":"Genetic polymorphism of the kinesin-like protein KIF1B gene and the risk of hepatocellular carcinoma.","date":"2013","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/23634229","citation_count":19,"is_preprint":false},{"pmid":"35960460","id":"PMC_35960460","title":"Circ_0003611 regulates apoptosis and oxidative stress injury of Alzheimer's disease via miR-383-5p/KIF1B axis.","date":"2022","source":"Metabolic brain disease","url":"https://pubmed.ncbi.nlm.nih.gov/35960460","citation_count":19,"is_preprint":false},{"pmid":"38883841","id":"PMC_38883841","title":"MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A.","date":"2024","source":"iScience","url":"https://pubmed.ncbi.nlm.nih.gov/38883841","citation_count":17,"is_preprint":false},{"pmid":"19744141","id":"PMC_19744141","title":"Altered motor activity of alternative splice variants of the mammalian kinesin-3 protein KIF1B.","date":"2009","source":"Traffic (Copenhagen, Denmark)","url":"https://pubmed.ncbi.nlm.nih.gov/19744141","citation_count":17,"is_preprint":false},{"pmid":"1536657","id":"PMC_1536657","title":"Characterization of a new kallikrein-like enzyme (KLP-S3) of the rat submandibular gland.","date":"1992","source":"The Biochemical journal","url":"https://pubmed.ncbi.nlm.nih.gov/1536657","citation_count":17,"is_preprint":false},{"pmid":"27696585","id":"PMC_27696585","title":"Loss of function of KIF1B impairs oocyte meiotic maturation and early embryonic development in mice.","date":"2016","source":"Molecular reproduction and development","url":"https://pubmed.ncbi.nlm.nih.gov/27696585","citation_count":17,"is_preprint":false},{"pmid":"36065637","id":"PMC_36065637","title":"Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12 -tubulin complex.","date":"2022","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/36065637","citation_count":16,"is_preprint":false},{"pmid":"22331516","id":"PMC_22331516","title":"Behavioral and molecular exploration of the AR-CMT2A mouse model Lmna (R298C/R298C).","date":"2012","source":"Neuromolecular medicine","url":"https://pubmed.ncbi.nlm.nih.gov/22331516","citation_count":16,"is_preprint":false},{"pmid":"10762626","id":"PMC_10762626","title":"Identification of the full-length KIAA0591 gene encoding a novel kinesin-related protein which is mapped to the neuroblastoma suppressor gene locus at 1p36.2.","date":"2000","source":"International journal of oncology","url":"https://pubmed.ncbi.nlm.nih.gov/10762626","citation_count":15,"is_preprint":false},{"pmid":"32245838","id":"PMC_32245838","title":"Defective nucleotide-dependent assembly and membrane fusion in Mfn2 CMT2A variants improved by Bax.","date":"2020","source":"Life science alliance","url":"https://pubmed.ncbi.nlm.nih.gov/32245838","citation_count":15,"is_preprint":false},{"pmid":"35924634","id":"PMC_35924634","title":"CMT2A-linked mitochondrial hyperfusion-driving mutant MFN2 perturbs ER-mitochondrial associations and Ca2+ homeostasis.","date":"2022","source":"Biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/35924634","citation_count":15,"is_preprint":false},{"pmid":"36556475","id":"PMC_36556475","title":"Mitochondria Clumping vs. Mitochondria Fusion in CMT2A Diseases.","date":"2022","source":"Life (Basel, Switzerland)","url":"https://pubmed.ncbi.nlm.nih.gov/36556475","citation_count":15,"is_preprint":false},{"pmid":"16270160","id":"PMC_16270160","title":"Stretchin-klp, a novel Drosophila indirect flight muscle protein, has both myosin dependent and independent isoforms.","date":"2005","source":"Journal of muscle research and cell motility","url":"https://pubmed.ncbi.nlm.nih.gov/16270160","citation_count":14,"is_preprint":false},{"pmid":"27122668","id":"PMC_27122668","title":"Effects of interactions between environmental factors and KIF1B genetic variants on the risk of hepatocellular carcinoma in a Chinese cohort.","date":"2016","source":"World journal of gastroenterology","url":"https://pubmed.ncbi.nlm.nih.gov/27122668","citation_count":14,"is_preprint":false},{"pmid":"9245592","id":"PMC_9245592","title":"Molecular cloning and expression of the Caenorhabditis elegans klp-3, an ortholog of C terminus motor kinesins Kar3 and ncd.","date":"1997","source":"Journal of molecular biology","url":"https://pubmed.ncbi.nlm.nih.gov/9245592","citation_count":13,"is_preprint":false},{"pmid":"34769001","id":"PMC_34769001","title":"Aberrant Mitochondrial Dynamics and Exacerbated Response to Neuroinflammation in a Novel Mouse Model of CMT2A.","date":"2021","source":"International journal of molecular sciences","url":"https://pubmed.ncbi.nlm.nih.gov/34769001","citation_count":13,"is_preprint":false},{"pmid":"17046997","id":"PMC_17046997","title":"Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells.","date":"2006","source":"Journal of cell science","url":"https://pubmed.ncbi.nlm.nih.gov/17046997","citation_count":13,"is_preprint":false},{"pmid":"35881155","id":"PMC_35881155","title":"Circ_0049472 regulates the damage of Aβ-induced SK-N-SH and CHP-212 cells by mediating the miR-107/KIF1B axis.","date":"2022","source":"Experimental brain research","url":"https://pubmed.ncbi.nlm.nih.gov/35881155","citation_count":12,"is_preprint":false},{"pmid":"32110117","id":"PMC_32110117","title":"CMT2A Harboring Mitofusin 2 Mutation with Optic Nerve Atrophy and Normal Visual Acuity.","date":"2020","source":"International medical case reports journal","url":"https://pubmed.ncbi.nlm.nih.gov/32110117","citation_count":12,"is_preprint":false},{"pmid":"20067515","id":"PMC_20067515","title":"Lack of replication of KIF1B gene in an Italian primary progressive multiple sclerosis cohort.","date":"2010","source":"European journal of neurology","url":"https://pubmed.ncbi.nlm.nih.gov/20067515","citation_count":12,"is_preprint":false},{"pmid":"15711769","id":"PMC_15711769","title":"Mutational study of the 1p located genes p18ink4c, Patched-2, RIZ1 and KIF1B in oligodendrogliomas.","date":"2005","source":"Oncology reports","url":"https://pubmed.ncbi.nlm.nih.gov/15711769","citation_count":11,"is_preprint":false},{"pmid":"34974134","id":"PMC_34974134","title":"CircPOSTN competes with KIF1B for miR-185-5p binding sites to promote the tumorigenesis of glioma.","date":"2021","source":"Brain research bulletin","url":"https://pubmed.ncbi.nlm.nih.gov/34974134","citation_count":10,"is_preprint":false},{"pmid":"28878341","id":"PMC_28878341","title":"A Botrytis cinerea KLP-7 Kinesin acts as a Virulence Determinant during Plant Infection.","date":"2017","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/28878341","citation_count":10,"is_preprint":false},{"pmid":"27706887","id":"PMC_27706887","title":"Increased mitochondrial fusion in a autosomal recessive CMT2A family with mitochondrial GTPase mitofusin 2 mutations.","date":"2016","source":"Journal of the peripheral nervous system : JPNS","url":"https://pubmed.ncbi.nlm.nih.gov/27706887","citation_count":10,"is_preprint":false},{"pmid":"15136675","id":"PMC_15136675","title":"Absence of KIF1B mutation in a large Turkish CMT2A family suggests involvement of a second gene.","date":"2004","source":"Neurology","url":"https://pubmed.ncbi.nlm.nih.gov/15136675","citation_count":9,"is_preprint":false},{"pmid":"34215911","id":"PMC_34215911","title":"Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease.","date":"2021","source":"Rice (New York, N.Y.)","url":"https://pubmed.ncbi.nlm.nih.gov/34215911","citation_count":9,"is_preprint":false},{"pmid":"33112832","id":"PMC_33112832","title":"Genetic predisposition to neural crest-derived tumors: revisiting the role of KIF1B.","date":"2020","source":"Endocrine connections","url":"https://pubmed.ncbi.nlm.nih.gov/33112832","citation_count":9,"is_preprint":false},{"pmid":"22762946","id":"PMC_22762946","title":"Mitofusin 2 gene mutation causing early-onset CMT2A with different progressive courses.","date":"2013","source":"Clinical neuropathology","url":"https://pubmed.ncbi.nlm.nih.gov/22762946","citation_count":9,"is_preprint":false},{"pmid":"10478423","id":"PMC_10478423","title":"Effects of dimerized lysozyme (KLP-602) on the cellular and humoral defence mechanisms in sheatfish (Silurus glanis): in vitro and in vivo study.","date":"1999","source":"Veterinary research","url":"https://pubmed.ncbi.nlm.nih.gov/10478423","citation_count":9,"is_preprint":false},{"pmid":"38206323","id":"PMC_38206323","title":"Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals a common and distinct activation mechanism in kinesin-3.","date":"2024","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/38206323","citation_count":8,"is_preprint":false},{"pmid":"32856204","id":"PMC_32856204","title":"Animal Models of CMT2A: State-of-art and Therapeutic Implications.","date":"2020","source":"Molecular neurobiology","url":"https://pubmed.ncbi.nlm.nih.gov/32856204","citation_count":7,"is_preprint":false},{"pmid":"15850386","id":"PMC_15850386","title":"Essential kinesins: characterization of Caenorhabditis elegans KLP-15.","date":"2005","source":"Biochemistry","url":"https://pubmed.ncbi.nlm.nih.gov/15850386","citation_count":7,"is_preprint":false},{"pmid":"36533211","id":"PMC_36533211","title":"Structure and biological evaluation of Caenorhabditis elegans CISD-1/mitoNEET, a KLP-17 tail domain homologue, supports attenuation of paraquat-induced oxidative stress through a p38 MAPK-mediated antioxidant defense response.","date":"2022","source":"Advances in redox research","url":"https://pubmed.ncbi.nlm.nih.gov/36533211","citation_count":7,"is_preprint":false},{"pmid":"28063088","id":"PMC_28063088","title":"Mosaicism for a pathogenic MFN2 mutation causes minimal clinical features of CMT2A in the parent of a severely affected child.","date":"2017","source":"Neurogenetics","url":"https://pubmed.ncbi.nlm.nih.gov/28063088","citation_count":7,"is_preprint":false},{"pmid":"38092249","id":"PMC_38092249","title":"CMT2A-linked MFN2 mutation, T206I promotes mitochondrial hyperfusion and predisposes cells towards mitophagy.","date":"2023","source":"Mitochondrion","url":"https://pubmed.ncbi.nlm.nih.gov/38092249","citation_count":6,"is_preprint":false},{"pmid":"35046208","id":"PMC_35046208","title":"A case of juvenile-onset pheochromocytoma with KIF1B p.V1529M germline mutation.","date":"2022","source":"Endocrine journal","url":"https://pubmed.ncbi.nlm.nih.gov/35046208","citation_count":6,"is_preprint":false},{"pmid":"32123843","id":"PMC_32123843","title":"The Kinesin-3 motor, KLP-4, mediates axonal organization and cholinergic signaling in Caenorhabditis elegans.","date":"2019","source":"FASEB bioAdvances","url":"https://pubmed.ncbi.nlm.nih.gov/32123843","citation_count":6,"is_preprint":false},{"pmid":"10819327","id":"PMC_10819327","title":"C. elegans KLP-11/OSM-3/KAP-1: orthologs of the sea urchin kinesin-II, and mouse KIF3A/KIFB/KAP3 kinesin complexes.","date":"2000","source":"DNA research : an international journal for rapid publication of reports on genes and genomes","url":"https://pubmed.ncbi.nlm.nih.gov/10819327","citation_count":6,"is_preprint":false},{"pmid":"29341354","id":"PMC_29341354","title":"A mutation in the heptad repeat 2 domain of MFN2 in a large CMT2A family.","date":"2018","source":"Journal of the peripheral nervous system : JPNS","url":"https://pubmed.ncbi.nlm.nih.gov/29341354","citation_count":5,"is_preprint":false},{"pmid":"38985513","id":"PMC_38985513","title":"KLP-7/Kinesin-13 orchestrates axon-dendrite checkpoints for polarized trafficking in neurons.","date":"2024","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/38985513","citation_count":4,"is_preprint":false},{"pmid":"10631116","id":"PMC_10631116","title":"cDNA cloning and expression of a C-terminus motor kinesin-like protein KLP-17, involved in chromosomal movement in Caenorhabditis elegans.","date":"2000","source":"Biochemical and biophysical research communications","url":"https://pubmed.ncbi.nlm.nih.gov/10631116","citation_count":4,"is_preprint":false},{"pmid":"40393993","id":"PMC_40393993","title":"An ultra-rare missense variant in the KIF1B gene linked to autoinflammatory Menière's disease.","date":"2025","source":"NPJ genomic medicine","url":"https://pubmed.ncbi.nlm.nih.gov/40393993","citation_count":3,"is_preprint":false},{"pmid":"37961478","id":"PMC_37961478","title":"Chromokinesin Klp-19 regulates microtubule overlap and dynamics during anaphase in C. elegans.","date":"2024","source":"bioRxiv : the preprint server for biology","url":"https://pubmed.ncbi.nlm.nih.gov/37961478","citation_count":3,"is_preprint":false},{"pmid":"35993942","id":"PMC_35993942","title":"tRNA-Derived Fragment tRF-18 Facilitates Cell Proliferation and Inhibits Cell Apoptosis via Modulating KIF1B in Papillary Thyroid Carcinoma.","date":"2022","source":"Critical reviews in eukaryotic gene expression","url":"https://pubmed.ncbi.nlm.nih.gov/35993942","citation_count":3,"is_preprint":false},{"pmid":"21680216","id":"PMC_21680216","title":"Polymorphic locus rs10492972 of the KIF1B gene association with multiple sclerosis in Russia: case control study.","date":"2011","source":"Molecular genetics and metabolism","url":"https://pubmed.ncbi.nlm.nih.gov/21680216","citation_count":3,"is_preprint":false},{"pmid":"26081705","id":"PMC_26081705","title":"[The relationship between the KIF1B (rs17401966) single nucleotide polymorphism and the genetic susceptibility to Hepatocellular carcinoma].","date":"2015","source":"Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]","url":"https://pubmed.ncbi.nlm.nih.gov/26081705","citation_count":3,"is_preprint":false},{"pmid":"37780619","id":"PMC_37780619","title":"Case Report: A 65-year-old man with paraganglioma accompanied by elevated interleukin-6 levels and KIF1B single gene mutation.","date":"2023","source":"Frontiers in endocrinology","url":"https://pubmed.ncbi.nlm.nih.gov/37780619","citation_count":2,"is_preprint":false},{"pmid":"30807887","id":"PMC_30807887","title":"Generation of induced pluripotent stem cell line, ZJUCHi002-A, from Charcot-Marie-Tooth disease type 2A (CMT2A) patient with a mutation of c.752C>T in MFN2.","date":"2019","source":"Stem cell research","url":"https://pubmed.ncbi.nlm.nih.gov/30807887","citation_count":2,"is_preprint":false},{"pmid":"41384344","id":"PMC_41384344","title":"KIF1B Regulates NLRP3-Mediated Pyroptosis in Asthma Progression.","date":"2025","source":"Journal of cellular and molecular medicine","url":"https://pubmed.ncbi.nlm.nih.gov/41384344","citation_count":1,"is_preprint":false},{"pmid":"39523115","id":"PMC_39523115","title":"Analysis of Expression of the GRIPAP1, DLG4, KIF1B, NGFRAP1, and NRF1 Genes in Peripheral Blood of the Patients with Parkinson's Disease in the Early Clinical Stages.","date":"2024","source":"Biochemistry. Biokhimiia","url":"https://pubmed.ncbi.nlm.nih.gov/39523115","citation_count":1,"is_preprint":false},{"pmid":"40612231","id":"PMC_40612231","title":"Modulating mitochondrial dynamics in CMT2A: a multifaceted platform for drug discovery and evaluation.","date":"2025","source":"Biophysics reports","url":"https://pubmed.ncbi.nlm.nih.gov/40612231","citation_count":1,"is_preprint":false},{"pmid":"38356673","id":"PMC_38356673","title":"CX‑5461 potentiates imatinib‑induced apoptosis in K562 cells by stimulating KIF1B expression.","date":"2024","source":"Experimental and therapeutic medicine","url":"https://pubmed.ncbi.nlm.nih.gov/38356673","citation_count":1,"is_preprint":false},{"pmid":"37564981","id":"PMC_37564981","title":"Case Report: Aggressive neural crest tumors in a child with familial von Hippel Lindau syndrome associated with a germline VHL mutation (c.414A>G) and a novel KIF1B gene mutation.","date":"2023","source":"Frontiers in endocrinology","url":"https://pubmed.ncbi.nlm.nih.gov/37564981","citation_count":1,"is_preprint":false},{"pmid":"41710718","id":"PMC_41710718","title":"The TRIM14-KIF1B Axis Drives Renal Injury in Diabetic Nephropathy Through TLR4/NF-κB Pathway Modulation.","date":"2026","source":"Diabetes, metabolic syndrome and obesity : targets and therapy","url":"https://pubmed.ncbi.nlm.nih.gov/41710718","citation_count":1,"is_preprint":false},{"pmid":"31127728","id":"PMC_31127728","title":"Late onset CMT2A in a Family with an MFN2 Variant: c.2222T>G (p.Leu741Trp).","date":"2019","source":"Journal of neuromuscular diseases","url":"https://pubmed.ncbi.nlm.nih.gov/31127728","citation_count":1,"is_preprint":false},{"pmid":"39604259","id":"PMC_39604259","title":"Biased movement of monomeric kinesin-3 KLP-6 explained by a symmetric Brownian ratchet model.","date":"2024","source":"Biophysical journal","url":"https://pubmed.ncbi.nlm.nih.gov/39604259","citation_count":0,"is_preprint":false},{"pmid":"41752078","id":"PMC_41752078","title":"AAVrh74.tMCK.NT-3 Surrogate Gene Therapy in a Mouse Model of CMT2A.","date":"2026","source":"International journal of molecular sciences","url":"https://pubmed.ncbi.nlm.nih.gov/41752078","citation_count":0,"is_preprint":false},{"pmid":"40917352","id":"PMC_40917352","title":"Case Report: Rare multisystem metastasis in head and neck paraganglioma with SDHB pathogenic variant and KIF1B VUS manifested as FUO.","date":"2025","source":"Frontiers in endocrinology","url":"https://pubmed.ncbi.nlm.nih.gov/40917352","citation_count":0,"is_preprint":false},{"pmid":"41789656","id":"PMC_41789656","title":"Exosomal circ0000549 promotes MNNG‑induced gastric cancer through miR‑15b‑5p/KIF1B.","date":"2026","source":"International journal of molecular medicine","url":"https://pubmed.ncbi.nlm.nih.gov/41789656","citation_count":0,"is_preprint":false},{"pmid":"42086734","id":"PMC_42086734","title":"KLP-6 is a kinesin superfamily protein resistant to ADP inhibition.","date":"2026","source":"Communications biology","url":"https://pubmed.ncbi.nlm.nih.gov/42086734","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":48567,"output_tokens":2941,"usd":0.094908,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":10379,"output_tokens":3644,"usd":0.071498,"stage2_stop_reason":"end_turn"},"total_usd":0.166406,"stage1_batch_id":"msgbatch_01BbTCMyrRPyhtEq6b1MdwCN","stage2_batch_id":"msgbatch_011dEed5bbxYvTAaQWKJevxu","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 1994,\n      \"finding\": \"KIF1B is a monomeric, microtubule plus end-directed motor protein that colocalizes with mitochondria in vivo, is concentrated in the mitochondrial subcellular fraction, and can transport mitochondria along microtubules in vitro.\",\n      \"method\": \"Rotary shadowing electron microscopy (monomeric structure), immunocytochemistry (colocalization with mitochondria), subcellular fractionation, in vitro microtubule-based transport assay\",\n      \"journal\": \"Cell\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 / Strong — in vitro reconstitution of mitochondrial transport, direct subcellular fractionation, and electron microscopy in a foundational study; independently cited and replicated concept\",\n      \"pmids\": [\"7528108\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 1999,\n      \"finding\": \"The Kif1b gene generates at least two major kinesin isoforms by alternative splicing: a shorter KIF1Bα (130 kDa) and a longer KIF1Bβ (204 kDa) with a novel C-terminal cargo-binding domain homologous to KIF1A, suggesting distinct cargo specificities.\",\n      \"method\": \"cDNA cloning, Northern blot analysis, haplotype mapping, alternative exon identification\",\n      \"journal\": \"Gene\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — molecular cloning with Northern blot across multiple tissues; functional cargo difference is inferred from sequence homology, not directly tested\",\n      \"pmids\": [\"10571041\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 1999,\n      \"finding\": \"The major brain isoform of Kif1b lacks the putative mitochondria-binding domain present in the originally described isoform, indicating that alternative splicing produces an isoform likely to have different cargo-binding specificity.\",\n      \"method\": \"cDNA library screening, Northern blot analysis, sequence comparison of C-terminal domains\",\n      \"journal\": \"Mammalian genome\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — molecular cloning and Northern blot; cargo-binding difference inferred from domain analysis, not functionally validated in transport assay\",\n      \"pmids\": [\"10341097\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2009,\n      \"finding\": \"Alternative splicing of KIF1B produces variants that differ in insertion sequences within the K-loop and hinge regions; variants containing both insertions show higher microtubule-dependent ATPase activity and microtubule affinity than those without insertions, as demonstrated by in vitro ATPase assays and mutational analysis of the K-loop.\",\n      \"method\": \"Recombinant protein expression, microtubule-dependent ATPase assay, in vitro motility assay, K-loop insertion mutagenesis\",\n      \"journal\": \"Traffic\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 / Moderate — in vitro ATPase reconstitution with mutagenesis of the K-loop in a single rigorous study; multiple orthogonal methods\",\n      \"pmids\": [\"19744141\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2009,\n      \"finding\": \"Kif1b is required for localization of myelin basic protein (mbp) mRNA to processes of myelinating oligodendrocytes in zebrafish; loss of kif1b causes ectopic appearance of myelin-like membrane and mislocalization of myelin proteins to oligodendrocyte cell bodies.\",\n      \"method\": \"Zebrafish kif1b mutant analysis, in vivo imaging, mRNA localization assays\",\n      \"journal\": \"Nature genetics\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — genetic loss-of-function in zebrafish with defined cellular phenotype (mRNA mislocalization and ectopic myelin), multiple readouts\",\n      \"pmids\": [\"19503091\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2009,\n      \"finding\": \"Kif1b is required for outgrowth of some of the longest axons in both peripheral and central nervous systems in zebrafish, establishing a role for this motor in axon elongation in vivo.\",\n      \"method\": \"Zebrafish kif1b mutant analysis, in vivo imaging of axon growth\",\n      \"journal\": \"Nature genetics\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — genetic loss-of-function in zebrafish with defined axon-length phenotype, multiple neuron types examined\",\n      \"pmids\": [\"19503091\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2013,\n      \"finding\": \"Leptin promotes gastric cancer cell invasion partly through KIF1B-dependent surface localization of MT1-MMP; co-immunoprecipitation showed leptin enhances the KIF1B–MT1-MMP interaction in a time-dependent manner, and siRNA knockdown of KIF1B inhibits leptin-induced membrane targeting of MT1-MMP.\",\n      \"method\": \"Co-immunoprecipitation, siRNA knockdown, cell surface biotinylation assay, flow cytometry, transwell invasion assay\",\n      \"journal\": \"Carcinogenesis\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2–3 / Moderate — reciprocal interaction by Co-IP plus functional siRNA knockdown with defined phenotype; single lab\",\n      \"pmids\": [\"23354307\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2015,\n      \"finding\": \"KIF1B promotes glioma cell migration and invasion through cell surface localization of MT1-MMP; siRNA knockdown of KIF1B reduces membranous MT1-MMP levels without altering total cellular MT1-MMP, consistent with a transport function.\",\n      \"method\": \"siRNA knockdown, transwell migration/invasion assay, cell surface MT1-MMP measurement\",\n      \"journal\": \"Oncology reports\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — siRNA knockdown with defined phenotype, corroborates gastric cancer finding; single lab, single primary method\",\n      \"pmids\": [\"26576027\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"The Kif1B motor and its binding partner KBP are required for anterograde transport of SCG10 (Stathmin-2) to axon growth cones in zebrafish; loss of KBP reduces SCG10 at growth cones, alters microtubule stability, and truncates axons—phenotypes rescued by SCG10 overexpression. Loss of Kif1B or KBP did not inhibit mitochondrial transport in this in vivo context.\",\n      \"method\": \"Zebrafish mutant analysis (kbp mutants), in vivo live imaging of SCG10 and microtubule dynamics, genetic epistasis (SCG10 overexpression rescue), mitochondrial transport imaging\",\n      \"journal\": \"The Journal of neuroscience\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — multiple orthogonal methods (live imaging, genetic rescue, microtubule stability assay), negative result for mitochondrial transport explicitly reported, replicated in zebrafish in vivo\",\n      \"pmids\": [\"27358458\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"KIF1B loss-of-function in mouse oocytes causes abnormal polar body extrusion, disordered spindle dynamics, chromosome congression defects, increased aneuploidy, and impaired embryonic development; KIF1B depletion also disrupts mitochondrial distribution and reduces ATP abundance in oocytes.\",\n      \"method\": \"siRNA-mediated KIF1B depletion, immunofluorescence for spindle assembly and chromosome alignment, mitochondrial distribution assay, ATP quantification\",\n      \"journal\": \"Molecular reproduction and development\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — loss-of-function with defined cellular phenotypes (spindle, chromosome, mitochondria), multiple readouts; single lab\",\n      \"pmids\": [\"27696585\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"KIF1B promotes NLRP3 inflammasome-mediated pyroptosis in asthma; KIF1B knockdown reduces expression of NLRP3, cleaved caspase-1, and cleaved GSDMD, and NLRP3 overexpression abolishes these protective effects, placing KIF1B upstream of NLRP3 in airway inflammatory signaling.\",\n      \"method\": \"siRNA knockdown, NLRP3 overexpression rescue, in vitro IL-13-stimulated airway epithelial cells, OVA-induced mouse asthma model, Western blot for pyroptosis markers\",\n      \"journal\": \"Journal of cellular and molecular medicine\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2–3 / Moderate — genetic epistasis (NLRP3 overexpression reversal), in vivo and in vitro models; single lab\",\n      \"pmids\": [\"41384344\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"TRIM14 directly interacts with KIF1B (confirmed by co-immunoprecipitation and immunofluorescence co-localization) and positively regulates KIF1B expression; this TRIM14–KIF1B axis drives renal tubular injury in diabetic nephropathy via TLR4/NF-κB pathway activation.\",\n      \"method\": \"Co-immunoprecipitation, immunofluorescence, siRNA knockdown of TRIM14, TRIM14 overexpression, Western blot, in vivo rat DN model, protein-protein interaction network analysis\",\n      \"journal\": \"Diabetes, metabolic syndrome and obesity\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2–3 / Moderate — Co-IP plus functional knockdown with pathway readout; single lab, in vivo and in vitro corroboration\",\n      \"pmids\": [\"41710718\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"KIF1B is a monomeric, microtubule plus-end-directed kinesin-3 motor that exists as multiple alternatively spliced isoforms with distinct cargo specificities: the originally described KIF1Bα isoform transports mitochondria along axonal microtubules, while the predominant KIF1Bβ isoform (lacking the mitochondria-binding domain) transports synaptic vesicle precursors and, together with its binding partner KBP, mediates anterograde transport of the microtubule regulator SCG10 to axon growth cones to control microtubule dynamics and axon elongation; KIF1B also localizes MT1-MMP to the cell surface in a transport-dependent manner promoting tumor invasion, is required in oligodendrocytes for mRNA localization of myelin basic protein to cellular processes, regulates spindle assembly and mitochondrial distribution during oocyte meiosis, and in the context of inflammatory signaling acts upstream of the NLRP3 inflammasome; the K-loop insertion in alternatively spliced variants modulates microtubule ATPase activity and affinity.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"KIF1B is a monomeric, microtubule plus-end-directed kinesin-3 motor that drives anterograde axonal transport and is required in vivo for the outgrowth of the longest peripheral and central axons [#0, #5]. The originally characterized isoform colocalizes with and transports mitochondria along microtubules in vitro [#0], while alternative splicing of the gene generates distinct isoforms—including a longer brain-enriched variant bearing a novel KIF1A-homologous C-terminal cargo-binding domain and a major isoform lacking the mitochondria-binding domain—that confer distinct cargo specificities [#1, #2]. Motor output is tuned at the mechanochemical level: splice variants differing in K-loop and hinge insertions display higher microtubule-dependent ATPase activity and microtubule affinity, with the K-loop directly modulating these properties [#3]. As a transport motor, KIF1B together with its binding partner KBP delivers the microtubule regulator SCG10/Stathmin-2 to axon growth cones to control microtubule stability and axon elongation, a function genetically separable from mitochondrial transport in vivo [#8], and it is also required for localization of myelin basic protein mRNA to oligodendrocyte processes [#4]. Beyond neural transport, KIF1B mediates cell-surface delivery of the matrix metalloproteinase MT1-MMP to promote tumor cell migration and invasion [#6, #7], regulates spindle dynamics, chromosome congression, and mitochondrial distribution during oocyte meiosis [#9], and acts upstream of NLRP3 inflammasome–driven pyroptosis in airway inflammation [#10].\",\n  \"teleology\": [\n    {\n      \"year\": 1994,\n      \"claim\": \"Established KIF1B as a bona fide motor protein with intrinsic cargo-transport capacity, answering whether it could physically move organelles along microtubules.\",\n      \"evidence\": \"Rotary-shadowing EM, subcellular fractionation, and in vitro microtubule-based transport reconstitution showing monomeric KIF1B transporting mitochondria\",\n      \"pmids\": [\"7528108\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Mechanism of cargo (mitochondria) attachment not defined\",\n        \"Regulation of motor activity in cells not addressed\",\n        \"Did not anticipate isoform diversity\"\n      ]\n    },\n    {\n      \"year\": 1999,\n      \"claim\": \"Revealed that the Kif1b locus produces multiple isoforms by alternative splicing with divergent C-terminal cargo-binding domains, reframing KIF1B as a family of motors with potentially distinct cargoes rather than a single mitochondrial transporter.\",\n      \"evidence\": \"cDNA cloning, Northern blot across tissues, and C-terminal domain sequence comparison identifying KIF1B\\u03b1, KIF1B\\u03b2, and a brain isoform lacking the mitochondria-binding domain\",\n      \"pmids\": [\"10571041\", \"10341097\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Distinct cargo specificities inferred from sequence, not tested in transport assays\",\n        \"Tissue-specific functions of each isoform unresolved\"\n      ]\n    },\n    {\n      \"year\": 2009,\n      \"claim\": \"Connected splice-variant sequence differences to motor mechanochemistry, showing the K-loop directly tunes microtubule binding and ATPase output.\",\n      \"evidence\": \"Recombinant protein microtubule-dependent ATPase and motility assays with K-loop insertion mutagenesis\",\n      \"pmids\": [\"19744141\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Physiological consequence of differential ATPase tuning in vivo not established\",\n        \"Does not link mechanochemical state to specific cargo selection\"\n      ]\n    },\n    {\n      \"year\": 2009,\n      \"claim\": \"Defined an in vivo neural requirement for KIF1B in long-axon outgrowth and in mRNA localization for myelination, extending its role beyond organelle transport to cargo delivery underpinning neural development.\",\n      \"evidence\": \"Zebrafish kif1b loss-of-function mutants with in vivo imaging of axon growth and mbp mRNA localization\",\n      \"pmids\": [\"19503091\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Molecular cargo delivered to support axon outgrowth not yet identified at this stage\",\n        \"Mechanism of mRNA cargo coupling unknown\"\n      ]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Identified SCG10/Stathmin-2 as a functional KIF1B cargo delivered via the partner KBP, mechanistically explaining the axon-outgrowth phenotype and dissociating it from mitochondrial transport.\",\n      \"evidence\": \"Zebrafish kbp mutants, live imaging of SCG10 and microtubule dynamics, SCG10-overexpression rescue, and explicit mitochondrial transport controls\",\n      \"pmids\": [\"27358458\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Direct biochemical KIF1B\\u2013KBP\\u2013SCG10 cargo complex not reconstituted\",\n        \"Relationship between KBP-dependent and mitochondrial transport functions not fully reconciled\"\n      ]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Extended KIF1B function to cell division, showing it governs meiotic spindle dynamics, chromosome congression, and mitochondrial distribution in oocytes.\",\n      \"evidence\": \"siRNA depletion in mouse oocytes with immunofluorescence of spindle/chromosomes, mitochondrial distribution, and ATP quantification\",\n      \"pmids\": [\"27696585\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct molecular targets at the spindle not identified\",\n        \"Single lab; isoform responsible not specified\",\n        \"Whether spindle defect is secondary to mitochondrial/ATP disruption unclear\"\n      ]\n    },\n    {\n      \"year\": 2013,\n      \"claim\": \"Implicated KIF1B in cancer cell invasion through transport-dependent surface delivery of MT1-MMP, generalizing its transport role to disease-relevant cargo.\",\n      \"evidence\": \"Co-IP, siRNA knockdown, cell-surface biotinylation, and transwell invasion assays in leptin-stimulated gastric cancer cells\",\n      \"pmids\": [\"23354307\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct vs indirect KIF1B\\u2013MT1-MMP interaction not fully resolved\",\n        \"Single lab at time of report\"\n      ]\n    },\n    {\n      \"year\": 2015,\n      \"claim\": \"Corroborated KIF1B-dependent MT1-MMP surface localization as a pro-invasive mechanism in a second tumor type, strengthening the transport interpretation.\",\n      \"evidence\": \"siRNA knockdown with surface vs total MT1-MMP measurement and migration/invasion assays in glioma cells\",\n      \"pmids\": [\"26576027\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single primary method (knockdown)\",\n        \"Mechanism of MT1-MMP cargo coupling unresolved\"\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Positioned KIF1B downstream of TRIM14 in an inflammatory signaling axis driving renal tubular injury, indicating regulated KIF1B expression in disease.\",\n      \"evidence\": \"Co-IP, immunofluorescence co-localization, TRIM14 knockdown/overexpression with TLR4/NF-\\u03baB readouts in a rat diabetic nephropathy model\",\n      \"pmids\": [\"41710718\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Whether KIF1B motor/transport activity is required for the phenotype not tested\",\n        \"Single lab\"\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Placed KIF1B upstream of NLRP3 inflammasome activation and pyroptosis in airway inflammation, expanding its role into innate immune signaling.\",\n      \"evidence\": \"siRNA knockdown with NLRP3-overexpression rescue and pyroptosis marker Western blots in IL-13-stimulated epithelial cells and an OVA mouse asthma model\",\n      \"pmids\": [\"41384344\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Mechanistic link between a microtubule motor and NLRP3 activation undefined\",\n        \"Single lab; direct interactors in this pathway not identified\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How distinct KIF1B splice isoforms select and couple their respective cargoes (mitochondria, synaptic vesicle precursors, SCG10/KBP, MT1-MMP, mRNAs) at the molecular level, and how the motor connects to inflammasome and spindle functions, remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No reconstituted isoform-specific cargo-adaptor complexes\",\n        \"Mechanistic basis for non-transport roles (NLRP3, spindle) unestablished\",\n        \"Isoform-resolved functional assignment across tissues incomplete\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003774\", \"supporting_discovery_ids\": [0, 3]},\n      {\"term_id\": \"GO:0140657\", \"supporting_discovery_ids\": [0, 3]},\n      {\"term_id\": \"GO:0008092\", \"supporting_discovery_ids\": [0, 3]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005856\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0005739\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0005886\", \"supporting_discovery_ids\": [6, 7]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-9609507\", \"supporting_discovery_ids\": [0, 8]},\n      {\"term_id\": \"R-HSA-5653656\", \"supporting_discovery_ids\": [0, 8]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"KBP\", \"SCG10\", \"MT1-MMP\", \"TRIM14\"],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":5,"faith_total":5,"faith_pct":100.0}}