{"gene":"KATNA1","run_date":"2026-06-10T01:55:23","timeline":{"discoveries":[{"year":2022,"finding":"KATNA1 contains a MIT domain that directly interacts with the ESCRT-III subunit CHMP3; this interaction recruits KATNA1 to cytokinetic midbody membrane bridges and is required for cytokinetic abscission.","method":"Comprehensive pairwise MIT–ESCRT-III tail binding assays (228 pairwise interactions quantified), localization to midbody bridges, and functional abscission assays","journal":"eLife","confidence":"High","confidence_rationale":"Tier 1–2 / Strong — quantitative binding measurements across all MIT–ESCRT-III pairs, localization experiment directly tied to functional abscission readout, multiple orthogonal methods in one rigorous study","pmids":["36107470"],"is_preprint":false},{"year":2022,"finding":"KATNA1 is SUMOylated at K330 by SUMO2; this modification enhances KATNA1-driven microtubule severing and promotes hippocampal neurite outgrowth. Mutation K330R abolishes both SUMOylation and the enhanced severing activity.","method":"Mass spectrometry identification of UBC9 in KATNA1 interactome; GST pull-down and co-immunoprecipitation for KATNA1–SUMO2 interaction; site-directed mutagenesis (K77R, K157R, K330R); microtubule-severing assay in COS7 cells; neurite outgrowth assay in hippocampal neurons","journal":"The Journal of biological chemistry","confidence":"High","confidence_rationale":"Tier 1–2 / Strong — multiple orthogonal methods (MS, Co-IP, GST pull-down, mutagenesis, cell-based severing assay, neuronal outgrowth assay) in one study establishing site-specific PTM and functional consequence","pmids":["35868557"],"is_preprint":false},{"year":2025,"finding":"KATNA1 interacts with CRMP3 via its MIT domain (residues 1–77) and the CRMP3 D region (residues 64–413); CRMP3 enhances KATNA1 microtubule-severing efficiency, and co-expression of both proteins in hippocampal neurons synergistically promotes neurite length and branching.","method":"GST pull-down, co-immunoprecipitation, domain-mapping experiments, microtubule-severing assay, neurite outgrowth assay in hippocampal neurons, KATNA1 and CRMP3 single and double knockout","journal":"Biochemical and biophysical research communications","confidence":"High","confidence_rationale":"Tier 1–2 / Moderate — reciprocal Co-IP plus GST pull-down, domain mapping, in-cell severing assay, and neuronal KO phenotype; single lab but multiple orthogonal methods","pmids":["39938451"],"is_preprint":false},{"year":2016,"finding":"KATNA1 (p60/A-subunit) is the catalytic microtubule-severing subunit of katanin; KATNBL1 associates with KATNA1 and regulates its microtubule-severing activity in vitro; KATNB1 can compete with KATNBL1 for binding to KATNA1.","method":"Mass spectrometry-based proteomic interactome mapping (Katan-ome); in vitro microtubule-severing assay; competition binding assay","journal":"Molecular & cellular proteomics : MCP","confidence":"High","confidence_rationale":"Tier 1–2 / Moderate — in vitro severing assay directly measuring KATNA1 activity, MS interactome, competition binding; multiple orthogonal methods in one study","pmids":["26929214"],"is_preprint":false},{"year":2014,"finding":"Disrupted interaction between mutant KATNB1 (regulatory subunit) and KATNA1 (catalytic subunit) underlies defective mitotic spindle formation in patient-derived fibroblasts carrying KATNB1 mutations.","method":"Exome sequencing of patient cohort; interaction studies between KATNB1 mutants and KATNA1 in patient-derived fibroblasts; mitotic spindle analysis","journal":"Neuron","confidence":"Medium","confidence_rationale":"Tier 2–3 / Moderate — interaction disruption shown in patient cells with spindle phenotype readout; methods described at abstract level without full reconstitution detail","pmids":["25521378"],"is_preprint":false},{"year":2019,"finding":"Constitutive homozygous Katna1 knockout is lethal in mice; haploinsufficiency causes accumulation of neuronal progenitors in the subventricular zone during corticogenesis and impairs progenitor proliferation in the adult hippocampal dentate gyrus subgranular zone, establishing KATNA1's role in neuronal progenitor proliferation.","method":"Katna1 knockout mouse generation; histological and BrdU/EdU proliferation analysis of SVZ and DG; behavioral testing","journal":"Scientific reports","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean KO/haploinsufficiency mouse model with defined cellular proliferation phenotype; single lab","pmids":["31685876"],"is_preprint":false},{"year":2023,"finding":"KATNA1 and its paralogue KATNAL1 cooperatively regulate the male meiotic spindle, cytokinesis, and midbody abscission, as well as spermatid remodeling events including Golgi organization, acrosome and manchette formation; proteomic mapping defines the KATNA1 testis interactome including cytoskeletal and vesicle trafficking proteins.","method":"Single and double gene knockout mice; histological and spermatogenic phenotype analysis; mass spectrometry-based testis interactome","journal":"Development (Cambridge, England)","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — multiple single and double KO models with defined spermatogenic phenotypes and MS interactome; single lab","pmids":["37882691"],"is_preprint":false},{"year":2025,"finding":"Oocyte-specific deletion of KATNA1 causes a ~50% decrease in fertility, a modest defect in MII spindle morphology, decreased fertilization rate, and impaired preimplantation embryo development; KATNAL1 deletion does not affect fertility, indicating KATNA1-specific roles in oocyte and early embryo function.","method":"ZP3-CreLox conditional knockout of KATNA1 and KATNAL1; spindle morphology analysis; fertilization rate measurement; parthenogenetic activation and blastocyst development assay","journal":"Molecular human reproduction","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean conditional KO with defined phenotypic readouts across multiple developmental stages; single lab","pmids":["40668235"],"is_preprint":false},{"year":2018,"finding":"KATNA1 (katanin A1/p60) shows ubiquitous tissue expression in mice and lower microtubule-severing activity compared to KATNAL1; the amino-terminal half of the protein determines its lower activity and faster intracellular degradation. KATNA1 knockdown in Neuro2a cells showed no effect on process elongation, in contrast to KATNAL1 knockdown.","method":"Tissue expression profiling; in-cell microtubule-severing assay comparing KATNA1 vs KATNAL1; cycloheximide chase protein stability assay; chimeric molecule domain-swap experiments; siRNA knockdown in Neuro2a cells","journal":"Biochemical and biophysical research communications","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — in-cell severing assay, domain-swap chimeras, stability assay, and KD phenotype; single lab with multiple orthogonal methods","pmids":["30448058"],"is_preprint":false},{"year":2019,"finding":"Elk1 transcription factor binds the KATNA1 5' UTR regulatory region; Elk1 overexpression increases KATNA1 mRNA but decreases katanin-p60 protein levels, indicating a post-transcriptional repressive mechanism. KATNA1 promoter methylation reduces Elk1 binding.","method":"KATNA1 5' UTR characterization; Elk1 overexpression with qRT-PCR (mRNA) and western blot (protein) in SH-SY5Y cells; methylation analysis of binding sites","journal":"PloS one","confidence":"Medium","confidence_rationale":"Tier 2–3 / Moderate — promoter/UTR binding and expression assays with both RNA and protein readouts; single lab","pmids":["30789974"],"is_preprint":false},{"year":2019,"finding":"p53 binds the KATNA1 promoter and transcriptionally activates KATNA1 gene expression; p53 overexpression increases both KATNA1 mRNA and katanin-p60 protein levels and alters the microtubule network in HCT 116 cells.","method":"KATNA1 promoter activity assays in HCT116 WT and p53(-/-) cells; p53–KATNA1 promoter ChIP/binding demonstration; p53 overexpression and knockdown with qRT-PCR and western blot; microtubule network imaging","journal":"Gene","confidence":"Medium","confidence_rationale":"Tier 2–3 / Moderate — promoter binding plus expression changes in isogenic p53 WT vs KO cell lines; single lab with multiple readouts","pmids":["31715301"],"is_preprint":false},{"year":2026,"finding":"EEF1B2 physically interacts with KATNA1 at its AAA+ ATPase domain and potentiates KATNA1-dependent microtubule severing in COS7 cells; EEF1B2 knockdown attenuates KATNA1-driven microtubule loss. In primary cortical neurons, EEF1B2 enhances KATNA1-induced neurite outgrowth and branching in a KATNA1-dependent manner.","method":"Proteomic screening; GST pull-down; co-immunoprecipitation; domain mapping to AAA+ ATPase domain; microtubule-severing assay in COS7; EEF1B2 knockdown; neurite outgrowth assay in primary cortical neurons","journal":"Neuroscience","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — GST pull-down plus Co-IP plus cell-based severing assay plus neuronal phenotype; single lab, multiple methods","pmids":["42002067"],"is_preprint":false},{"year":2023,"finding":"miR-124-3p reduces KATNA1 mRNA and katanin-p60 protein levels in SH-SY5Y neuroblastoma cells, suggesting post-transcriptional regulation of KATNA1 expression.","method":"Bioinformatic prediction of miR-124-3p binding to KATNA1 mRNA; transfection of pre-miR-124-3p mimics; qRT-PCR and western blot for KATNA1 expression","journal":"Cytoskeleton (Hoboken, N.J.)","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single method (transfection + expression readout), single lab, no direct 3'-UTR reporter or RISC pull-down to confirm direct miRNA targeting","pmids":["37439368"],"is_preprint":false}],"current_model":"KATNA1 encodes katanin p60, the catalytic AAA+ ATPase subunit of the katanin microtubule-severing enzyme, which severs microtubules at its K330 SUMOylation site under SUMO2 regulation; its activity is enhanced by binding partners CRMP3 (via its MIT domain) and EEF1B2 (via its AAA+ domain), and it is recruited to cytokinetic midbody bridges through a MIT–CHMP3 interaction to execute abscission; at the transcriptional level, p53 activates and Elk1 differentially modulates KATNA1 expression, while its protein-level activity is coordinated with the regulatory subunit KATNB1 and competitor KATNBL1, collectively governing neuronal progenitor proliferation, spindle assembly, meiosis, and neurite outgrowth."},"narrative":{"mechanistic_narrative":"KATNA1 encodes katanin p60, the catalytic AAA+ ATPase subunit that drives microtubule severing and thereby governs cytoskeletal remodeling in mitotic and meiotic spindle assembly, cytokinesis, and neuronal morphogenesis [PMID:26929214, PMID:31685876, PMID:37882691]. Its severing activity is tuned by accessory subunits: KATNBL1 and the regulatory subunit KATNB1 compete for binding to KATNA1 and modulate its activity in vitro, and disruption of the KATNB1–KATNA1 interaction underlies defective mitotic spindle formation in patient fibroblasts [PMID:26929214, PMID:25521378]. Severing output is further potentiated by direct partners that engage distinct domains — CRMP3 and EEF1B2 bind the MIT domain (residues 1–77) and the AAA+ ATPase domain respectively to enhance severing and synergistically promote neurite outgrowth and branching [PMID:39938451, PMID:42002067] — and by SUMO2 conjugation at K330, which augments severing and hippocampal neurite outgrowth [PMID:35868557]. Through a MIT-domain interaction with the ESCRT-III subunit CHMP3, KATNA1 is recruited to cytokinetic midbody bridges to execute abscission [PMID:36107470]. Transcriptionally, p53 binds the KATNA1 promoter and activates its expression, while Elk1 and miR-124-3p impose post-transcriptional repression of katanin-p60 protein [PMID:31715301, PMID:30789974]. Genetically, KATNA1 is essential in mice, with haploinsufficiency impairing neuronal progenitor proliferation, and it acts both cooperatively with its paralogue KATNAL1 in male meiosis and spermatid remodeling and in a KATNAL1-independent manner in oocyte spindle function and early embryo development [PMID:31685876, PMID:37882691, PMID:40668235].","teleology":[{"year":2014,"claim":"Established that the physical KATNA1–KATNB1 interaction is functionally required for mitotic spindle assembly, linking katanin subunit assembly to human disease.","evidence":"Exome sequencing of a patient cohort plus KATNB1-mutant/KATNA1 interaction studies and spindle analysis in patient-derived fibroblasts","pmids":["25521378"],"confidence":"Medium","gaps":["No reconstitution defining how the interaction modulates KATNA1 catalytic activity","Mechanism linking spindle defect to specific developmental phenotype unresolved"]},{"year":2016,"claim":"Defined the regulatory architecture of katanin by showing KATNBL1 modulates KATNA1 severing activity and competes with KATNB1 for binding the catalytic subunit.","evidence":"Mass spectrometry interactome (Katan-ome), in vitro microtubule-severing assay, and competition binding assay","pmids":["26929214"],"confidence":"High","gaps":["Quantitative balance between KATNB1 and KATNBL1 occupancy in vivo not established","Structural basis of competition unknown"]},{"year":2018,"claim":"Distinguished KATNA1 from its paralogue KATNAL1, attributing KATNA1's lower severing activity and faster turnover to its N-terminal half.","evidence":"Tissue expression profiling, in-cell severing assays, cycloheximide-chase stability assay, domain-swap chimeras, and siRNA knockdown in Neuro2a cells","pmids":["30448058"],"confidence":"Medium","gaps":["Degradation pathway controlling protein stability not identified","No effect on Neuro2a process elongation leaves cell-type specificity open"]},{"year":2019,"claim":"Identified opposing transcriptional/post-transcriptional control of KATNA1 by p53 (activation) and Elk1 (mRNA up, protein down), placing katanin under regulatory checkpoints.","evidence":"Promoter binding/ChIP and expression assays in isogenic p53 WT vs KO HCT116 cells, and Elk1 5'UTR binding with mRNA/protein readouts plus methylation analysis in SH-SY5Y cells","pmids":["31715301","30789974"],"confidence":"Medium","gaps":["Mechanism of Elk1-driven post-transcriptional repression undefined","Integration of p53 and Elk1 inputs in a single cell context untested"]},{"year":2019,"claim":"Established KATNA1 as essential and dose-sensitive for neuronal progenitor proliferation in vivo.","evidence":"Constitutive Katna1 knockout and haploinsufficient mice with BrdU/EdU proliferation analysis of SVZ and DG and behavioral testing","pmids":["31685876"],"confidence":"Medium","gaps":["Molecular link between severing activity and progenitor proliferation not dissected","Cell-autonomous vs non-autonomous contribution unresolved"]},{"year":2022,"claim":"Revealed how KATNA1 is spatially targeted to the abscission machinery via a direct MIT–CHMP3 (ESCRT-III) interaction required for cytokinetic abscission.","evidence":"Quantitative pairwise MIT–ESCRT-III tail binding assays, midbody localization, and functional abscission assays","pmids":["36107470"],"confidence":"High","gaps":["Whether severing at the midbody is required for abscission, versus a scaffolding role, not separated","Temporal coordination with ESCRT polymerization unknown"]},{"year":2022,"claim":"Demonstrated a site-specific PTM (SUMO2 at K330) that directly enhances severing and neurite outgrowth, adding a covalent activity switch.","evidence":"MS interactome (UBC9), GST pull-down/Co-IP, K77R/K157R/K330R mutagenesis, severing assay in COS7, and hippocampal neurite outgrowth assay","pmids":["35868557"],"confidence":"High","gaps":["Structural mechanism by which SUMOylation enhances ATPase/severing unknown","Physiological signals triggering K330 SUMOylation unidentified"]},{"year":2023,"claim":"Mapped paralogue-specific and shared roles of KATNA1 and KATNAL1 in male meiosis, cytokinesis, and spermatid remodeling, and defined the testis interactome.","evidence":"Single and double knockout mice with spermatogenic phenotyping and MS-based testis interactome","pmids":["37882691"],"confidence":"Medium","gaps":["Direct substrates among spermatid structures not pinpointed","Functional validation of interactome hits beyond catalog lacking"]},{"year":2023,"claim":"Proposed miR-124-3p as a post-transcriptional repressor of KATNA1 in neuroblastoma cells.","evidence":"Bioinformatic prediction plus pre-miR-124-3p mimic transfection with qRT-PCR and western blot in SH-SY5Y cells","pmids":["37439368"],"confidence":"Low","gaps":["No 3'-UTR reporter or RISC pull-down to confirm direct targeting","Single method, single cell line","Physiological relevance untested"]},{"year":2025,"claim":"Identified CRMP3 as a MIT-domain partner that enhances severing and synergizes with KATNA1 to promote neurite length and branching.","evidence":"GST pull-down, reciprocal Co-IP, domain mapping (MIT 1–77 / CRMP3 D region 64–413), in-cell severing assay, and single/double KO neurite outgrowth assays","pmids":["39938451"],"confidence":"High","gaps":["Mechanism by which CRMP3 binding stimulates the AAA+ ATPase cycle unknown","Whether CRMP3 competes with CHMP3 for the shared MIT domain untested"]},{"year":2025,"claim":"Established KATNA1-specific (KATNAL1-independent) requirements in oocyte spindle morphology and early embryo development.","evidence":"ZP3-CreLox conditional knockout of KATNA1 and KATNAL1 with spindle analysis, fertilization-rate measurement, and blastocyst/parthenogenetic development assays","pmids":["40668235"],"confidence":"Medium","gaps":["Molecular basis of the MII spindle defect not resolved","Source of preimplantation developmental failure (maternal vs embryonic) unclear"]},{"year":2026,"claim":"Identified EEF1B2 as an AAA+-domain partner that potentiates severing and enhances KATNA1-dependent neurite outgrowth, extending the catalog of activity-enhancing partners that bind distinct KATNA1 domains.","evidence":"Proteomic screen, GST pull-down, Co-IP, domain mapping to the AAA+ ATPase domain, severing assay in COS7, EEF1B2 knockdown, and neurite outgrowth in primary cortical neurons","pmids":["42002067"],"confidence":"Medium","gaps":["Whether EEF1B2 acts on nucleotide cycling versus oligomerization unknown","Relationship to canonical EEF1B2 translation-factor role unexplored"]},{"year":null,"claim":"How the multiple activity-enhancing inputs (KATNB1/KATNBL1 occupancy, K330 SUMOylation, CRMP3, EEF1B2) are integrated to set katanin severing output in a given cell type, and what microtubule substrates are targeted in each developmental context, remain open.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No integrated structural/biochemical model of combinatorial regulation","Physiological substrate specificity across spindle, midbody, and neurite contexts undefined"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0140657","term_label":"ATP-dependent activity","supporting_discovery_ids":[3,11]},{"term_id":"GO:0140096","term_label":"catalytic activity, acting on a protein","supporting_discovery_ids":[3,1,2,11]},{"term_id":"GO:0008092","term_label":"cytoskeletal protein binding","supporting_discovery_ids":[3,8]},{"term_id":"GO:0016787","term_label":"hydrolase activity","supporting_discovery_ids":[3]}],"localization":[{"term_id":"GO:0005856","term_label":"cytoskeleton","supporting_discovery_ids":[3,10]},{"term_id":"GO:0005815","term_label":"microtubule organizing center","supporting_discovery_ids":[0]}],"pathway":[{"term_id":"R-HSA-1640170","term_label":"Cell Cycle","supporting_discovery_ids":[4,3]},{"term_id":"R-HSA-1266738","term_label":"Developmental Biology","supporting_discovery_ids":[5,2]},{"term_id":"R-HSA-1474165","term_label":"Reproduction","supporting_discovery_ids":[6,7]}],"complexes":["katanin (p60/p80 microtubule-severing complex)"],"partners":["KATNB1","KATNBL1","CHMP3","CRMP3","EEF1B2","SUMO2","UBC9"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"O75449","full_name":"Katanin p60 ATPase-containing subunit A1","aliases":["p60 katanin"],"length_aa":491,"mass_kda":56.0,"function":"Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth","subcellular_location":"Cytoplasm; Midbody; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, spindle","url":"https://www.uniprot.org/uniprotkb/O75449/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/KATNA1","classification":"Not Classified","n_dependent_lines":17,"n_total_lines":1208,"dependency_fraction":0.014072847682119206},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"PSMD9","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/KATNA1","total_profiled":1310},"omim":[{"mim_id":"618919","title":"KELCH-LIKE FAMILY, MEMBER 42; KLHL42","url":"https://www.omim.org/entry/618919"},{"mim_id":"616650","title":"KATANIN-INTERACTING PROTEIN; KATNIP","url":"https://www.omim.org/entry/616650"},{"mim_id":"616212","title":"LISSENCEPHALY 6 WITH MICROCEPHALY; LIS6","url":"https://www.omim.org/entry/616212"},{"mim_id":"614764","title":"KATANIN, p60 SUBUNIT, A-LIKE 1; KATNAL1","url":"https://www.omim.org/entry/614764"},{"mim_id":"610454","title":"LEUCINE ZIPPER, PUTATIVE TUMOR SUPPRESSOR 2; LZTS2","url":"https://www.omim.org/entry/610454"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Supported","locations":[{"location":"Centriolar satellite","reliability":"Supported"},{"location":"Nucleoplasm","reliability":"Additional"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/KATNA1"},"hgnc":{"alias_symbol":[],"prev_symbol":[]},"alphafold":{"accession":"O75449","domains":[{"cath_id":"1.20.58.80","chopping":"4-76","consensus_level":"high","plddt":88.9882,"start":4,"end":76},{"cath_id":"3.40.50.300","chopping":"190-379","consensus_level":"high","plddt":81.6471,"start":190,"end":379},{"cath_id":"1.10.8.60","chopping":"385-473","consensus_level":"high","plddt":88.1583,"start":385,"end":473}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/O75449","model_url":"https://alphafold.ebi.ac.uk/files/AF-O75449-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-O75449-F1-predicted_aligned_error_v6.png","plddt_mean":75.0},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=KATNA1","jax_strain_url":"https://www.jax.org/strain/search?query=KATNA1"},"sequence":{"accession":"O75449","fasta_url":"https://rest.uniprot.org/uniprotkb/O75449.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/O75449/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/O75449"}},"corpus_meta":[{"pmid":"25521378","id":"PMC_25521378","title":"Mutations in KATNB1 cause complex cerebral malformations by disrupting asymmetrically dividing neural progenitors.","date":"2014","source":"Neuron","url":"https://pubmed.ncbi.nlm.nih.gov/25521378","citation_count":89,"is_preprint":false},{"pmid":"30094535","id":"PMC_30094535","title":"Clinical relevance of cytoskeleton associated proteins for ovarian cancer.","date":"2018","source":"Journal of cancer research and clinical oncology","url":"https://pubmed.ncbi.nlm.nih.gov/30094535","citation_count":42,"is_preprint":false},{"pmid":"26929214","id":"PMC_26929214","title":"Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing.","date":"2016","source":"Molecular & cellular proteomics : MCP","url":"https://pubmed.ncbi.nlm.nih.gov/26929214","citation_count":42,"is_preprint":false},{"pmid":"36107470","id":"PMC_36107470","title":"Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.","date":"2022","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/36107470","citation_count":28,"is_preprint":false},{"pmid":"35760404","id":"PMC_35760404","title":"Genome-wide association analysis of nine reproduction and morphological traits in three goat breeds from Southern China.","date":"2022","source":"Animal bioscience","url":"https://pubmed.ncbi.nlm.nih.gov/35760404","citation_count":16,"is_preprint":false},{"pmid":"28791777","id":"PMC_28791777","title":"Mutations in the Katnb1 gene cause left-right asymmetry and heart defects.","date":"2017","source":"Developmental dynamics : an official publication of the American Association of Anatomists","url":"https://pubmed.ncbi.nlm.nih.gov/28791777","citation_count":12,"is_preprint":false},{"pmid":"35868557","id":"PMC_35868557","title":"SUMOylation of microtubule-cleaving enzyme KATNA1 promotes microtubule severing and neurite outgrowth.","date":"2022","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/35868557","citation_count":11,"is_preprint":false},{"pmid":"30448058","id":"PMC_30448058","title":"KATNAL1 is a more active and stable isoform of katanin, and is expressed dominantly in neurons.","date":"2018","source":"Biochemical and biophysical research communications","url":"https://pubmed.ncbi.nlm.nih.gov/30448058","citation_count":11,"is_preprint":false},{"pmid":"31685876","id":"PMC_31685876","title":"The Microtubule Severing Protein Katanin Regulates Proliferation of Neuronal Progenitors in Embryonic and Adult Neurogenesis.","date":"2019","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/31685876","citation_count":11,"is_preprint":false},{"pmid":"32506788","id":"PMC_32506788","title":"Cardiomyocyte Proteome Remodeling due to Isoproterenol-Induced Cardiac Hypertrophy during the Compensated Phase.","date":"2020","source":"Proteomics. 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this interaction recruits KATNA1 to cytokinetic midbody membrane bridges and is required for cytokinetic abscission.\",\n      \"method\": \"Comprehensive pairwise MIT–ESCRT-III tail binding assays (228 pairwise interactions quantified), localization to midbody bridges, and functional abscission assays\",\n      \"journal\": \"eLife\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Strong — quantitative binding measurements across all MIT–ESCRT-III pairs, localization experiment directly tied to functional abscission readout, multiple orthogonal methods in one rigorous study\",\n      \"pmids\": [\"36107470\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"KATNA1 is SUMOylated at K330 by SUMO2; this modification enhances KATNA1-driven microtubule severing and promotes hippocampal neurite outgrowth. Mutation K330R abolishes both SUMOylation and the enhanced severing activity.\",\n      \"method\": \"Mass spectrometry identification of UBC9 in KATNA1 interactome; GST pull-down and co-immunoprecipitation for KATNA1–SUMO2 interaction; site-directed mutagenesis (K77R, K157R, K330R); microtubule-severing assay in COS7 cells; neurite outgrowth assay in hippocampal neurons\",\n      \"journal\": \"The Journal of biological chemistry\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Strong — multiple orthogonal methods (MS, Co-IP, GST pull-down, mutagenesis, cell-based severing assay, neuronal outgrowth assay) in one study establishing site-specific PTM and functional consequence\",\n      \"pmids\": [\"35868557\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"KATNA1 interacts with CRMP3 via its MIT domain (residues 1–77) and the CRMP3 D region (residues 64–413); CRMP3 enhances KATNA1 microtubule-severing efficiency, and co-expression of both proteins in hippocampal neurons synergistically promotes neurite length and branching.\",\n      \"method\": \"GST pull-down, co-immunoprecipitation, domain-mapping experiments, microtubule-severing assay, neurite outgrowth assay in hippocampal neurons, KATNA1 and CRMP3 single and double knockout\",\n      \"journal\": \"Biochemical and biophysical research communications\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Moderate — reciprocal Co-IP plus GST pull-down, domain mapping, in-cell severing assay, and neuronal KO phenotype; single lab but multiple orthogonal methods\",\n      \"pmids\": [\"39938451\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"KATNA1 (p60/A-subunit) is the catalytic microtubule-severing subunit of katanin; KATNBL1 associates with KATNA1 and regulates its microtubule-severing activity in vitro; KATNB1 can compete with KATNBL1 for binding to KATNA1.\",\n      \"method\": \"Mass spectrometry-based proteomic interactome mapping (Katan-ome); in vitro microtubule-severing assay; competition binding assay\",\n      \"journal\": \"Molecular & cellular proteomics : MCP\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Moderate — in vitro severing assay directly measuring KATNA1 activity, MS interactome, competition binding; multiple orthogonal methods in one study\",\n      \"pmids\": [\"26929214\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2014,\n      \"finding\": \"Disrupted interaction between mutant KATNB1 (regulatory subunit) and KATNA1 (catalytic subunit) underlies defective mitotic spindle formation in patient-derived fibroblasts carrying KATNB1 mutations.\",\n      \"method\": \"Exome sequencing of patient cohort; interaction studies between KATNB1 mutants and KATNA1 in patient-derived fibroblasts; mitotic spindle analysis\",\n      \"journal\": \"Neuron\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2–3 / Moderate — interaction disruption shown in patient cells with spindle phenotype readout; methods described at abstract level without full reconstitution detail\",\n      \"pmids\": [\"25521378\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"Constitutive homozygous Katna1 knockout is lethal in mice; haploinsufficiency causes accumulation of neuronal progenitors in the subventricular zone during corticogenesis and impairs progenitor proliferation in the adult hippocampal dentate gyrus subgranular zone, establishing KATNA1's role in neuronal progenitor proliferation.\",\n      \"method\": \"Katna1 knockout mouse generation; histological and BrdU/EdU proliferation analysis of SVZ and DG; behavioral testing\",\n      \"journal\": \"Scientific reports\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO/haploinsufficiency mouse model with defined cellular proliferation phenotype; single lab\",\n      \"pmids\": [\"31685876\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"KATNA1 and its paralogue KATNAL1 cooperatively regulate the male meiotic spindle, cytokinesis, and midbody abscission, as well as spermatid remodeling events including Golgi organization, acrosome and manchette formation; proteomic mapping defines the KATNA1 testis interactome including cytoskeletal and vesicle trafficking proteins.\",\n      \"method\": \"Single and double gene knockout mice; histological and spermatogenic phenotype analysis; mass spectrometry-based testis interactome\",\n      \"journal\": \"Development (Cambridge, England)\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — multiple single and double KO models with defined spermatogenic phenotypes and MS interactome; single lab\",\n      \"pmids\": [\"37882691\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Oocyte-specific deletion of KATNA1 causes a ~50% decrease in fertility, a modest defect in MII spindle morphology, decreased fertilization rate, and impaired preimplantation embryo development; KATNAL1 deletion does not affect fertility, indicating KATNA1-specific roles in oocyte and early embryo function.\",\n      \"method\": \"ZP3-CreLox conditional knockout of KATNA1 and KATNAL1; spindle morphology analysis; fertilization rate measurement; parthenogenetic activation and blastocyst development assay\",\n      \"journal\": \"Molecular human reproduction\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean conditional KO with defined phenotypic readouts across multiple developmental stages; single lab\",\n      \"pmids\": [\"40668235\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"KATNA1 (katanin A1/p60) shows ubiquitous tissue expression in mice and lower microtubule-severing activity compared to KATNAL1; the amino-terminal half of the protein determines its lower activity and faster intracellular degradation. KATNA1 knockdown in Neuro2a cells showed no effect on process elongation, in contrast to KATNAL1 knockdown.\",\n      \"method\": \"Tissue expression profiling; in-cell microtubule-severing assay comparing KATNA1 vs KATNAL1; cycloheximide chase protein stability assay; chimeric molecule domain-swap experiments; siRNA knockdown in Neuro2a cells\",\n      \"journal\": \"Biochemical and biophysical research communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — in-cell severing assay, domain-swap chimeras, stability assay, and KD phenotype; single lab with multiple orthogonal methods\",\n      \"pmids\": [\"30448058\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"Elk1 transcription factor binds the KATNA1 5' UTR regulatory region; Elk1 overexpression increases KATNA1 mRNA but decreases katanin-p60 protein levels, indicating a post-transcriptional repressive mechanism. KATNA1 promoter methylation reduces Elk1 binding.\",\n      \"method\": \"KATNA1 5' UTR characterization; Elk1 overexpression with qRT-PCR (mRNA) and western blot (protein) in SH-SY5Y cells; methylation analysis of binding sites\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2–3 / Moderate — promoter/UTR binding and expression assays with both RNA and protein readouts; single lab\",\n      \"pmids\": [\"30789974\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"p53 binds the KATNA1 promoter and transcriptionally activates KATNA1 gene expression; p53 overexpression increases both KATNA1 mRNA and katanin-p60 protein levels and alters the microtubule network in HCT 116 cells.\",\n      \"method\": \"KATNA1 promoter activity assays in HCT116 WT and p53(-/-) cells; p53–KATNA1 promoter ChIP/binding demonstration; p53 overexpression and knockdown with qRT-PCR and western blot; microtubule network imaging\",\n      \"journal\": \"Gene\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2–3 / Moderate — promoter binding plus expression changes in isogenic p53 WT vs KO cell lines; single lab with multiple readouts\",\n      \"pmids\": [\"31715301\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"EEF1B2 physically interacts with KATNA1 at its AAA+ ATPase domain and potentiates KATNA1-dependent microtubule severing in COS7 cells; EEF1B2 knockdown attenuates KATNA1-driven microtubule loss. In primary cortical neurons, EEF1B2 enhances KATNA1-induced neurite outgrowth and branching in a KATNA1-dependent manner.\",\n      \"method\": \"Proteomic screening; GST pull-down; co-immunoprecipitation; domain mapping to AAA+ ATPase domain; microtubule-severing assay in COS7; EEF1B2 knockdown; neurite outgrowth assay in primary cortical neurons\",\n      \"journal\": \"Neuroscience\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — GST pull-down plus Co-IP plus cell-based severing assay plus neuronal phenotype; single lab, multiple methods\",\n      \"pmids\": [\"42002067\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"miR-124-3p reduces KATNA1 mRNA and katanin-p60 protein levels in SH-SY5Y neuroblastoma cells, suggesting post-transcriptional regulation of KATNA1 expression.\",\n      \"method\": \"Bioinformatic prediction of miR-124-3p binding to KATNA1 mRNA; transfection of pre-miR-124-3p mimics; qRT-PCR and western blot for KATNA1 expression\",\n      \"journal\": \"Cytoskeleton (Hoboken, N.J.)\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single method (transfection + expression readout), single lab, no direct 3'-UTR reporter or RISC pull-down to confirm direct miRNA targeting\",\n      \"pmids\": [\"37439368\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"KATNA1 encodes katanin p60, the catalytic AAA+ ATPase subunit of the katanin microtubule-severing enzyme, which severs microtubules at its K330 SUMOylation site under SUMO2 regulation; its activity is enhanced by binding partners CRMP3 (via its MIT domain) and EEF1B2 (via its AAA+ domain), and it is recruited to cytokinetic midbody bridges through a MIT–CHMP3 interaction to execute abscission; at the transcriptional level, p53 activates and Elk1 differentially modulates KATNA1 expression, while its protein-level activity is coordinated with the regulatory subunit KATNB1 and competitor KATNBL1, collectively governing neuronal progenitor proliferation, spindle assembly, meiosis, and neurite outgrowth.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"KATNA1 encodes katanin p60, the catalytic AAA+ ATPase subunit that drives microtubule severing and thereby governs cytoskeletal remodeling in mitotic and meiotic spindle assembly, cytokinesis, and neuronal morphogenesis [#3, #5, #6]. Its severing activity is tuned by accessory subunits: KATNBL1 and the regulatory subunit KATNB1 compete for binding to KATNA1 and modulate its activity in vitro, and disruption of the KATNB1–KATNA1 interaction underlies defective mitotic spindle formation in patient fibroblasts [#3, #4]. Severing output is further potentiated by direct partners that engage distinct domains — CRMP3 and EEF1B2 bind the MIT domain (residues 1–77) and the AAA+ ATPase domain respectively to enhance severing and synergistically promote neurite outgrowth and branching [#2, #11] — and by SUMO2 conjugation at K330, which augments severing and hippocampal neurite outgrowth [#1]. Through a MIT-domain interaction with the ESCRT-III subunit CHMP3, KATNA1 is recruited to cytokinetic midbody bridges to execute abscission [#0]. Transcriptionally, p53 binds the KATNA1 promoter and activates its expression, while Elk1 and miR-124-3p impose post-transcriptional repression of katanin-p60 protein [#10, #9]. Genetically, KATNA1 is essential in mice, with haploinsufficiency impairing neuronal progenitor proliferation, and it acts both cooperatively with its paralogue KATNAL1 in male meiosis and spermatid remodeling and in a KATNAL1-independent manner in oocyte spindle function and early embryo development [#5, #6, #7].\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Established that the physical KATNA1–KATNB1 interaction is functionally required for mitotic spindle assembly, linking katanin subunit assembly to human disease.\",\n      \"evidence\": \"Exome sequencing of a patient cohort plus KATNB1-mutant/KATNA1 interaction studies and spindle analysis in patient-derived fibroblasts\",\n      \"pmids\": [\"25521378\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"No reconstitution defining how the interaction modulates KATNA1 catalytic activity\", \"Mechanism linking spindle defect to specific developmental phenotype unresolved\"]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Defined the regulatory architecture of katanin by showing KATNBL1 modulates KATNA1 severing activity and competes with KATNB1 for binding the catalytic subunit.\",\n      \"evidence\": \"Mass spectrometry interactome (Katan-ome), in vitro microtubule-severing assay, and competition binding assay\",\n      \"pmids\": [\"26929214\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Quantitative balance between KATNB1 and KATNBL1 occupancy in vivo not established\", \"Structural basis of competition unknown\"]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Distinguished KATNA1 from its paralogue KATNAL1, attributing KATNA1's lower severing activity and faster turnover to its N-terminal half.\",\n      \"evidence\": \"Tissue expression profiling, in-cell severing assays, cycloheximide-chase stability assay, domain-swap chimeras, and siRNA knockdown in Neuro2a cells\",\n      \"pmids\": [\"30448058\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Degradation pathway controlling protein stability not identified\", \"No effect on Neuro2a process elongation leaves cell-type specificity open\"]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"Identified opposing transcriptional/post-transcriptional control of KATNA1 by p53 (activation) and Elk1 (mRNA up, protein down), placing katanin under regulatory checkpoints.\",\n      \"evidence\": \"Promoter binding/ChIP and expression assays in isogenic p53 WT vs KO HCT116 cells, and Elk1 5'UTR binding with mRNA/protein readouts plus methylation analysis in SH-SY5Y cells\",\n      \"pmids\": [\"31715301\", \"30789974\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Mechanism of Elk1-driven post-transcriptional repression undefined\", \"Integration of p53 and Elk1 inputs in a single cell context untested\"]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"Established KATNA1 as essential and dose-sensitive for neuronal progenitor proliferation in vivo.\",\n      \"evidence\": \"Constitutive Katna1 knockout and haploinsufficient mice with BrdU/EdU proliferation analysis of SVZ and DG and behavioral testing\",\n      \"pmids\": [\"31685876\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Molecular link between severing activity and progenitor proliferation not dissected\", \"Cell-autonomous vs non-autonomous contribution unresolved\"]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"Revealed how KATNA1 is spatially targeted to the abscission machinery via a direct MIT–CHMP3 (ESCRT-III) interaction required for cytokinetic abscission.\",\n      \"evidence\": \"Quantitative pairwise MIT–ESCRT-III tail binding assays, midbody localization, and functional abscission assays\",\n      \"pmids\": [\"36107470\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Whether severing at the midbody is required for abscission, versus a scaffolding role, not separated\", \"Temporal coordination with ESCRT polymerization unknown\"]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"Demonstrated a site-specific PTM (SUMO2 at K330) that directly enhances severing and neurite outgrowth, adding a covalent activity switch.\",\n      \"evidence\": \"MS interactome (UBC9), GST pull-down/Co-IP, K77R/K157R/K330R mutagenesis, severing assay in COS7, and hippocampal neurite outgrowth assay\",\n      \"pmids\": [\"35868557\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Structural mechanism by which SUMOylation enhances ATPase/severing unknown\", \"Physiological signals triggering K330 SUMOylation unidentified\"]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Mapped paralogue-specific and shared roles of KATNA1 and KATNAL1 in male meiosis, cytokinesis, and spermatid remodeling, and defined the testis interactome.\",\n      \"evidence\": \"Single and double knockout mice with spermatogenic phenotyping and MS-based testis interactome\",\n      \"pmids\": [\"37882691\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Direct substrates among spermatid structures not pinpointed\", \"Functional validation of interactome hits beyond catalog lacking\"]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Proposed miR-124-3p as a post-transcriptional repressor of KATNA1 in neuroblastoma cells.\",\n      \"evidence\": \"Bioinformatic prediction plus pre-miR-124-3p mimic transfection with qRT-PCR and western blot in SH-SY5Y cells\",\n      \"pmids\": [\"37439368\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No 3'-UTR reporter or RISC pull-down to confirm direct targeting\", \"Single method, single cell line\", \"Physiological relevance untested\"]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Identified CRMP3 as a MIT-domain partner that enhances severing and synergizes with KATNA1 to promote neurite length and branching.\",\n      \"evidence\": \"GST pull-down, reciprocal Co-IP, domain mapping (MIT 1–77 / CRMP3 D region 64–413), in-cell severing assay, and single/double KO neurite outgrowth assays\",\n      \"pmids\": [\"39938451\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Mechanism by which CRMP3 binding stimulates the AAA+ ATPase cycle unknown\", \"Whether CRMP3 competes with CHMP3 for the shared MIT domain untested\"]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Established KATNA1-specific (KATNAL1-independent) requirements in oocyte spindle morphology and early embryo development.\",\n      \"evidence\": \"ZP3-CreLox conditional knockout of KATNA1 and KATNAL1 with spindle analysis, fertilization-rate measurement, and blastocyst/parthenogenetic development assays\",\n      \"pmids\": [\"40668235\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Molecular basis of the MII spindle defect not resolved\", \"Source of preimplantation developmental failure (maternal vs embryonic) unclear\"]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Identified EEF1B2 as an AAA+-domain partner that potentiates severing and enhances KATNA1-dependent neurite outgrowth, extending the catalog of activity-enhancing partners that bind distinct KATNA1 domains.\",\n      \"evidence\": \"Proteomic screen, GST pull-down, Co-IP, domain mapping to the AAA+ ATPase domain, severing assay in COS7, EEF1B2 knockdown, and neurite outgrowth in primary cortical neurons\",\n      \"pmids\": [\"42002067\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Whether EEF1B2 acts on nucleotide cycling versus oligomerization unknown\", \"Relationship to canonical EEF1B2 translation-factor role unexplored\"]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How the multiple activity-enhancing inputs (KATNB1/KATNBL1 occupancy, K330 SUMOylation, CRMP3, EEF1B2) are integrated to set katanin severing output in a given cell type, and what microtubule substrates are targeted in each developmental context, remain open.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"No integrated structural/biochemical model of combinatorial regulation\", \"Physiological substrate specificity across spindle, midbody, and neurite contexts undefined\"]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140657\", \"supporting_discovery_ids\": [3, 11]},\n      {\"term_id\": \"GO:0140096\", \"supporting_discovery_ids\": [3, 1, 2, 11]},\n      {\"term_id\": \"GO:0008092\", \"supporting_discovery_ids\": [3, 8]},\n      {\"term_id\": \"GO:0016787\", \"supporting_discovery_ids\": [3]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005856\", \"supporting_discovery_ids\": [3, 10]},\n      {\"term_id\": \"GO:0005815\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-1640170\", \"supporting_discovery_ids\": [4, 3]},\n      {\"term_id\": \"R-HSA-1266738\", \"supporting_discovery_ids\": [5, 2]},\n      {\"term_id\": \"R-HSA-1474165\", \"supporting_discovery_ids\": [6, 7]}\n    ],\n    \"complexes\": [\"katanin (p60/p80 microtubule-severing complex)\"],\n    \"partners\": [\"KATNB1\", \"KATNBL1\", \"CHMP3\", \"CRMP3\", \"EEF1B2\", \"SUMO2\", \"UBC9\"],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":6,"faith_total":6,"faith_pct":100.0}}