{"gene":"HAUS2","run_date":"2026-06-10T01:55:21","timeline":{"discoveries":[{"year":2017,"finding":"HAUS2 is a component of the Augmin/HAUS complex; combinatorial knockdown of HAUS2 together with HAUS5 in neural progenitor cells (NPCs) causes centrosome fragmentation and lethality, demonstrating that HAUS2 functionally contributes to Augmin/HAUS complex activity required for centrosome integrity and cell viability.","method":"siRNA knockdown (combinatorial KD of HAUS2 + HAUS5) with phenotypic readouts of centrosome fragmentation and cell viability in NPCs","journal":"Oncotarget","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — single lab, genetic epistasis via combinatorial knockdown with defined cellular phenotype, but no in vitro reconstitution or structural data","pmids":["28596487"],"is_preprint":false}],"current_model":"HAUS2 is a subunit of the Augmin/HAUS complex that supports microtubule nucleation along the mitotic spindle and maintains centrosome integrity; loss of HAUS2 in combination with other Augmin subunits (e.g., HAUS5) causes centrosome fragmentation and cell death, indicating it contributes to essential Augmin complex activity in dividing cells."},"narrative":{"mechanistic_narrative":"HAUS2 is a subunit of the Augmin/HAUS complex that supports microtubule nucleation along the mitotic spindle and maintains centrosome integrity in dividing cells [PMID:28596487]. Combinatorial knockdown of HAUS2 together with the Augmin subunit HAUS5 in neural progenitor cells causes centrosome fragmentation and cell death, demonstrating that HAUS2 functionally contributes to essential Augmin/HAUS complex activity required for centrosome integrity and viability [PMID:28596487]. Beyond this genetic epistasis, no further mechanistic detail — including biochemical reconstitution, structural assembly within the complex, or direct microtubule-binding activity of HAUS2 itself — has been characterized in the available corpus.","teleology":[{"year":2017,"claim":"Established that HAUS2 is not merely an annotated complex member but functionally contributes to Augmin/HAUS activity required for centrosome integrity and cell survival.","evidence":"Combinatorial siRNA knockdown of HAUS2 with HAUS5 in neural progenitor cells, scored for centrosome fragmentation and viability","pmids":["28596487"],"confidence":"Medium","gaps":["No in vitro reconstitution or structural data defining HAUS2's role within the complex","Single-lab study relying on combinatorial knockdown rather than isolated HAUS2 loss","Direct molecular activity of HAUS2 (e.g., microtubule binding or nucleation contribution) not measured"]},{"year":null,"claim":"How HAUS2 contributes biochemically to Augmin-dependent microtubule nucleation and what its direct binding partners and structural position within the complex are remain unresolved.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No structural model of HAUS2 within the Augmin/HAUS complex","No reconstituted assay isolating HAUS2's molecular function","No direct demonstration of HAUS2-microtubule interaction"]}],"mechanism_profile":{"molecular_activity":[],"localization":[{"term_id":"GO:0005815","term_label":"microtubule organizing center","supporting_discovery_ids":[0]}],"pathway":[{"term_id":"R-HSA-1640170","term_label":"Cell Cycle","supporting_discovery_ids":[0]}],"complexes":["Augmin/HAUS complex"],"partners":["HAUS5"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q9NVX0","full_name":"HAUS augmin-like complex subunit 2","aliases":["Centrosomal protein of 27 kDa","Cep27"],"length_aa":235,"mass_kda":26.9,"function":"Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex","subcellular_location":"Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle","url":"https://www.uniprot.org/uniprotkb/Q9NVX0/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":true,"resolved_as":"","url":"https://depmap.org/portal/gene/HAUS2","classification":"Common Essential","n_dependent_lines":420,"n_total_lines":1208,"dependency_fraction":0.347682119205298},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"ARFIP1","stoichiometry":0.2},{"gene":"SMC1A","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/HAUS2","total_profiled":1310},"omim":[{"mim_id":"613429","title":"HAUS AUGMIN-LIKE COMPLEX, SUBUNIT 2; HAUS2","url":"https://www.omim.org/entry/613429"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Nucleoplasm","reliability":"Approved"},{"location":"Cytosol","reliability":"Approved"},{"location":"Centrosome","reliability":"Additional"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in all","driving_tissues":[{"tissue":"bone marrow","ntpm":34.4}],"url":"https://www.proteinatlas.org/search/HAUS2"},"hgnc":{"alias_symbol":["FLJ10460","HsT17025"],"prev_symbol":["C15orf25","CEP27"]},"alphafold":{"accession":"Q9NVX0","domains":[{"cath_id":"1.20.5","chopping":"19-79","consensus_level":"medium","plddt":86.107,"start":19,"end":79}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q9NVX0","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q9NVX0-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q9NVX0-F1-predicted_aligned_error_v6.png","plddt_mean":80.5},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=HAUS2","jax_strain_url":"https://www.jax.org/strain/search?query=HAUS2"},"sequence":{"accession":"Q9NVX0","fasta_url":"https://rest.uniprot.org/uniprotkb/Q9NVX0.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q9NVX0/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q9NVX0"}},"corpus_meta":[{"pmid":"23456457","id":"PMC_23456457","title":"CEP proteins: the knights of centrosome dynasty.","date":"2013","source":"Protoplasma","url":"https://pubmed.ncbi.nlm.nih.gov/23456457","citation_count":81,"is_preprint":false},{"pmid":"28596487","id":"PMC_28596487","title":"ZNF131 suppresses centrosome fragmentation in glioblastoma stem-like cells through regulation of HAUS5.","date":"2017","source":"Oncotarget","url":"https://pubmed.ncbi.nlm.nih.gov/28596487","citation_count":15,"is_preprint":false},{"pmid":"35559016","id":"PMC_35559016","title":"Brain Epitranscriptomic Analysis Revealed Altered A-to-I RNA Editing in Septic Patients.","date":"2022","source":"Frontiers in genetics","url":"https://pubmed.ncbi.nlm.nih.gov/35559016","citation_count":7,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3713,"output_tokens":366,"usd":0.008315,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6603,"output_tokens":932,"usd":0.028157,"stage2_stop_reason":"end_turn"},"total_usd":0.036472,"stage1_batch_id":"msgbatch_01DUPACZi7AAWwuZiUjseQF4","stage2_batch_id":"msgbatch_018yt7chE3f48PEFtDwtj6AE","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"HAUS2 is a component of the Augmin/HAUS complex; combinatorial knockdown of HAUS2 together with HAUS5 in neural progenitor cells (NPCs) causes centrosome fragmentation and lethality, demonstrating that HAUS2 functionally contributes to Augmin/HAUS complex activity required for centrosome integrity and cell viability.\",\n      \"method\": \"siRNA knockdown (combinatorial KD of HAUS2 + HAUS5) with phenotypic readouts of centrosome fragmentation and cell viability in NPCs\",\n      \"journal\": \"Oncotarget\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — single lab, genetic epistasis via combinatorial knockdown with defined cellular phenotype, but no in vitro reconstitution or structural data\",\n      \"pmids\": [\"28596487\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"HAUS2 is a subunit of the Augmin/HAUS complex that supports microtubule nucleation along the mitotic spindle and maintains centrosome integrity; loss of HAUS2 in combination with other Augmin subunits (e.g., HAUS5) causes centrosome fragmentation and cell death, indicating it contributes to essential Augmin complex activity in dividing cells.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"HAUS2 is a subunit of the Augmin/HAUS complex that supports microtubule nucleation along the mitotic spindle and maintains centrosome integrity in dividing cells [#0]. Combinatorial knockdown of HAUS2 together with the Augmin subunit HAUS5 in neural progenitor cells causes centrosome fragmentation and cell death, demonstrating that HAUS2 functionally contributes to essential Augmin/HAUS complex activity required for centrosome integrity and viability [#0]. Beyond this genetic epistasis, no further mechanistic detail — including biochemical reconstitution, structural assembly within the complex, or direct microtubule-binding activity of HAUS2 itself — has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"Established that HAUS2 is not merely an annotated complex member but functionally contributes to Augmin/HAUS activity required for centrosome integrity and cell survival.\",\n      \"evidence\": \"Combinatorial siRNA knockdown of HAUS2 with HAUS5 in neural progenitor cells, scored for centrosome fragmentation and viability\",\n      \"pmids\": [\"28596487\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No in vitro reconstitution or structural data defining HAUS2's role within the complex\",\n        \"Single-lab study relying on combinatorial knockdown rather than isolated HAUS2 loss\",\n        \"Direct molecular activity of HAUS2 (e.g., microtubule binding or nucleation contribution) not measured\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How HAUS2 contributes biochemically to Augmin-dependent microtubule nucleation and what its direct binding partners and structural position within the complex are remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No structural model of HAUS2 within the Augmin/HAUS complex\",\n        \"No reconstituted assay isolating HAUS2's molecular function\",\n        \"No direct demonstration of HAUS2-microtubule interaction\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0005815\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-1640170\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"complexes\": [\"Augmin/HAUS complex\"],\n    \"partners\": [\"HAUS5\"],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":2,"faith_total":2,"faith_pct":100.0}}