{"gene":"FASTK","run_date":"2026-04-28T17:46:03","timeline":{"discoveries":[{"year":1995,"finding":"FASTK (Fas-activated serine/threonine kinase) is rapidly dephosphorylated and activated upon Fas ligation, and the activated kinase phosphorylates TIA-1, a nuclear RNA-binding protein implicated as an effector of apoptosis; phosphorylation of TIA-1 precedes DNA fragmentation, placing FASTK upstream of apoptotic DNA fragmentation.","method":"In vitro kinase assay, immunoprecipitation, phosphorylation studies in Fas-ligated Jurkat cells","journal":"The Journal of experimental medicine","confidence":"High","confidence_rationale":"Tier 2 — original discovery paper with direct biochemical demonstration of substrate phosphorylation and pathway placement via temporal analysis","pmids":["7544399"],"is_preprint":false},{"year":2007,"finding":"FASTK localizes to the outer mitochondrial membrane under normal conditions and translocates to stress granules upon environmental stress, where it interacts with TIA-1 to modulate stress-induced translational silencing; FASTK is also found in the nucleus at nuclear speckles and promotes inclusion of exon IIIb of FGFR2 mRNA by acting on a U-rich intronic sequence (IAS1) independently of TIA-1.","method":"Two-hybrid interaction screens, biochemical co-immunoprecipitation, siRNA knockdown, minigene splicing assays, subcellular fractionation and immunofluorescence localization","journal":"Proceedings of the National Academy of Sciences of the United States of America","confidence":"High","confidence_rationale":"Tier 2 — multiple orthogonal methods including yeast two-hybrid, biochemical pulldown, RNAi knockdown with functional splicing readout, and localization in a single study","pmids":["17592127"],"is_preprint":false},{"year":2017,"finding":"FASTK family proteins (FASTK and FASTKD1-5) are post-transcriptional regulators of mitochondrial gene expression; each member has distinct roles in mRNA processing and maturation, ribosome assembly, and translation within mitochondria, consistent with their shared RAP domain architecture enabling RNA binding.","method":"Review synthesizing biochemical, genetic, and cell-biology studies of the FASTK family","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 3 — review consolidating multiple studies but no single new experiment presented","pmids":["29036396"],"is_preprint":false},{"year":2021,"finding":"FASTK family members, particularly FASTKD4 and FASTKD5, are required for non-canonical RNA junction processing of the human mitochondrial transcriptome; loss of FASTKD5 causes severe defects in mitochondrial translation of electron transport chain components and oxidative phosphorylation, and combined knockouts increase specific antisense transcripts.","method":"CRISPR/Cas9 knockout of single and combined FASTK family members in human cell lines, comprehensive mitochondrial transcriptome sequencing, mitochondrial translation assays, oxidative phosphorylation measurements","journal":"PLoS genetics","confidence":"High","confidence_rationale":"Tier 2 — clean genetic KO with multiple orthogonal functional readouts (transcriptomics, translation, respiration) in human cells","pmids":["34748562"],"is_preprint":false},{"year":2013,"finding":"FASTK is a direct target of miR-106a-5p in astrocytoma cells; knockdown of FASTK inhibits cell proliferation and migration and promotes apoptosis, phenocopying miR-106a-5p overexpression, establishing FASTK as a downstream effector mediating these cellular effects.","method":"Luciferase reporter assay confirming direct miRNA targeting, western blot, siRNA knockdown with proliferation/migration/apoptosis assays","journal":"PloS one","confidence":"Medium","confidence_rationale":"Tier 2 — direct target validation by reporter assay combined with functional loss-of-function phenotype","pmids":["24013584"],"is_preprint":false}],"current_model":"FASTK is a mitochondria-associated serine/threonine kinase that, upon Fas-mediated apoptotic signaling, is dephosphorylated and activated to phosphorylate TIA-1 upstream of DNA fragmentation; it also shuttles to stress granules to modulate translational silencing and to nuclear speckles where it promotes alternative splicing of FGFR2; as the founding member of the FASTK family, it functions as a post-transcriptional regulator of mitochondrial RNA processing, with its homologs collectively governing non-canonical junction processing, ribosome assembly, and translation within mitochondria."},"narrative":{"teleology":[{"year":1995,"claim":"The identification of FASTK as a Fas-activated kinase that phosphorylates TIA-1 established the first direct biochemical link between Fas death-receptor signaling and a downstream kinase–substrate pair operating upstream of DNA fragmentation.","evidence":"In vitro kinase assays and immunoprecipitation in Fas-ligated Jurkat cells","pmids":["7544399"],"confidence":"High","gaps":["The structural basis of FASTK kinase activity and its activation by dephosphorylation remains undefined","Whether TIA-1 phosphorylation is necessary and sufficient for downstream DNA fragmentation was not tested","The kinase(s) and phosphatase(s) that regulate FASTK phosphorylation status are unknown"]},{"year":2007,"claim":"Demonstration that FASTK resides on the outer mitochondrial membrane yet dynamically relocates to stress granules and nuclear speckles revealed it as a tri-compartmental protein with separable functions in translational silencing (via TIA-1) and alternative splicing (TIA-1-independent promotion of FGFR2 exon IIIb inclusion).","evidence":"Two-hybrid screens, co-immunoprecipitation, siRNA knockdown, minigene splicing assays, and immunofluorescence in mammalian cells","pmids":["17592127"],"confidence":"High","gaps":["The signals triggering FASTK redistribution among the three compartments are not characterized","The mechanism by which FASTK recognizes the IAS1 U-rich element to promote exon inclusion is unresolved","Whether the kinase activity of FASTK is required for its splicing or stress-granule functions was not determined"]},{"year":2013,"claim":"Validation of FASTK as a direct miR-106a-5p target whose depletion suppresses proliferation and migration while promoting apoptosis in astrocytoma cells placed FASTK in a pro-survival, pro-proliferative role in a cancer context.","evidence":"Luciferase reporter assay for direct miRNA targeting, siRNA knockdown with proliferation, migration, and apoptosis assays in astrocytoma cells","pmids":["24013584"],"confidence":"Medium","gaps":["The downstream effectors through which FASTK promotes proliferation and migration were not identified","Findings are from a single cancer cell type without in vivo validation","Whether the kinase activity or RNA-regulatory functions of FASTK mediate these phenotypes is unknown"]},{"year":2017,"claim":"A systematic review consolidated evidence that the FASTK family shares a RAP domain enabling RNA binding and collectively governs mitochondrial mRNA processing, ribosome assembly, and translation, reframing FASTK as the founding member of a mitochondrial RNA-regulatory family.","evidence":"Review synthesizing biochemical, genetic, and cell-biology studies across FASTK family members","pmids":["29036396"],"confidence":"Medium","gaps":["The specific mitochondrial RNA targets of FASTK itself (as opposed to its paralogs) were not delineated","No structural data for any FASTK-family RAP domain were available","Whether FASTK and its paralogs function redundantly or in a coordinated manner was not resolved"]},{"year":2021,"claim":"CRISPR knockouts demonstrated that FASTK family members, especially FASTKD4 and FASTKD5, are required for non-canonical RNA junction processing of the mitochondrial transcriptome, with FASTKD5 loss causing severe translation and oxidative phosphorylation defects, providing direct genetic evidence for the family's essential role in mitochondrial gene expression.","evidence":"Single and combinatorial CRISPR/Cas9 knockouts in human cell lines with mitochondrial transcriptome sequencing, translation assays, and respiration measurements","pmids":["34748562"],"confidence":"High","gaps":["The specific contribution of FASTK itself (versus FASTKD4/D5) to junction processing was not isolated","The mechanism by which individual family members recognize their target RNA junctions is unknown","In vivo physiological consequences of FASTK family loss in animal models have not been reported"]},{"year":null,"claim":"A key unresolved question is how the kinase activity, RNA-regulatory functions, and dynamic subcellular redistribution of FASTK are coordinated, and which specific mitochondrial RNA targets FASTK itself processes independently of its paralogs.","evidence":"","pmids":[],"confidence":"Low","gaps":["No structural model of full-length FASTK or its RAP domain exists","The relationship between FASTK kinase activity and its RNA-regulatory roles has never been tested","Specific mitochondrial transcript targets of FASTK (not paralogs) remain to be defined by loss-of-function approaches"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0140096","term_label":"catalytic activity, acting on a protein","supporting_discovery_ids":[0]},{"term_id":"GO:0003723","term_label":"RNA binding","supporting_discovery_ids":[1,2]}],"localization":[{"term_id":"GO:0005739","term_label":"mitochondrion","supporting_discovery_ids":[1,2,3]},{"term_id":"GO:0005634","term_label":"nucleus","supporting_discovery_ids":[1]},{"term_id":"GO:0005829","term_label":"cytosol","supporting_discovery_ids":[1]}],"pathway":[{"term_id":"R-HSA-5357801","term_label":"Programmed Cell Death","supporting_discovery_ids":[0,4]},{"term_id":"R-HSA-8953854","term_label":"Metabolism of RNA","supporting_discovery_ids":[2,3]},{"term_id":"R-HSA-74160","term_label":"Gene expression (Transcription)","supporting_discovery_ids":[1]}],"complexes":[],"partners":["TIA1"],"other_free_text":[]},"mechanistic_narrative":"FASTK is a multifunctional signaling kinase and RNA-processing regulator that operates across mitochondrial, cytoplasmic, and nuclear compartments. Upon Fas receptor ligation, FASTK is dephosphorylated and activated, whereupon it phosphorylates the RNA-binding protein TIA-1 upstream of apoptotic DNA fragmentation [PMID:7544399]. Under basal conditions FASTK localizes to the outer mitochondrial membrane, but upon stress it translocates to cytoplasmic stress granules to modulate translational silencing via TIA-1 interaction, and also resides in nuclear speckles where it promotes FGFR2 exon IIIb inclusion through a U-rich intronic element independently of TIA-1 [PMID:17592127]. As the founding member of the FASTK family, it shares a RAP domain architecture that enables RNA binding and participates in post-transcriptional regulation of mitochondrial gene expression, with family members collectively governing non-canonical RNA junction processing, ribosome assembly, and mitochondrial translation [PMID:29036396, PMID:34748562]."},"prefetch_data":{"uniprot":{"accession":"Q14296","full_name":"Fas-activated serine/threonine kinase","aliases":[],"length_aa":549,"mass_kda":61.1,"function":"Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels","subcellular_location":"Mitochondrion matrix","url":"https://www.uniprot.org/uniprotkb/Q14296/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/FASTK","classification":"Not Classified","n_dependent_lines":0,"n_total_lines":1208,"dependency_fraction":0.0},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/FASTK","total_profiled":1310},"omim":[{"mim_id":"617530","title":"FAST KINASE DOMAINS 3; FASTKD3","url":"https://www.omim.org/entry/617530"},{"mim_id":"614272","title":"FAST KINASE DOMAINS 5; FASTKD5","url":"https://www.omim.org/entry/614272"},{"mim_id":"606965","title":"FAS-ACTIVATED SERINE/THREONINE KINASE; FASTK","url":"https://www.omim.org/entry/606965"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Supported","locations":[{"location":"Mitochondria","reliability":"Supported"},{"location":"Nuclear speckles","reliability":"Additional"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/FASTK"},"hgnc":{"alias_symbol":["FAST"],"prev_symbol":[]},"alphafold":{"accession":"Q14296","domains":[{"cath_id":"-","chopping":"85-186","consensus_level":"high","plddt":82.2243,"start":85,"end":186},{"cath_id":"-","chopping":"390-456_471-539","consensus_level":"high","plddt":86.5626,"start":390,"end":539},{"cath_id":"1.25.40","chopping":"230-380","consensus_level":"high","plddt":89.7085,"start":230,"end":380}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q14296","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q14296-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q14296-F1-predicted_aligned_error_v6.png","plddt_mean":73.81},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=FASTK","jax_strain_url":"https://www.jax.org/strain/search?query=FASTK"},"sequence":{"accession":"Q14296","fasta_url":"https://rest.uniprot.org/uniprotkb/Q14296.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q14296/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q14296"}},"corpus_meta":[{"pmid":"25402007","id":"PMC_25402007","title":"Fast 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phosphorylation of TIA-1 precedes DNA fragmentation, placing FASTK upstream of apoptotic DNA fragmentation.\",\n      \"method\": \"In vitro kinase assay, immunoprecipitation, phosphorylation studies in Fas-ligated Jurkat cells\",\n      \"journal\": \"The Journal of experimental medicine\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — original discovery paper with direct biochemical demonstration of substrate phosphorylation and pathway placement via temporal analysis\",\n      \"pmids\": [\"7544399\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2007,\n      \"finding\": \"FASTK localizes to the outer mitochondrial membrane under normal conditions and translocates to stress granules upon environmental stress, where it interacts with TIA-1 to modulate stress-induced translational silencing; FASTK is also found in the nucleus at nuclear speckles and promotes inclusion of exon IIIb of FGFR2 mRNA by acting on a U-rich intronic sequence (IAS1) independently of TIA-1.\",\n      \"method\": \"Two-hybrid interaction screens, biochemical co-immunoprecipitation, siRNA knockdown, minigene splicing assays, subcellular fractionation and immunofluorescence localization\",\n      \"journal\": \"Proceedings of the National Academy of Sciences of the United States of America\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — multiple orthogonal methods including yeast two-hybrid, biochemical pulldown, RNAi knockdown with functional splicing readout, and localization in a single study\",\n      \"pmids\": [\"17592127\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"FASTK family proteins (FASTK and FASTKD1-5) are post-transcriptional regulators of mitochondrial gene expression; each member has distinct roles in mRNA processing and maturation, ribosome assembly, and translation within mitochondria, consistent with their shared RAP domain architecture enabling RNA binding.\",\n      \"method\": \"Review synthesizing biochemical, genetic, and cell-biology studies of the FASTK family\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 — review consolidating multiple studies but no single new experiment presented\",\n      \"pmids\": [\"29036396\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"FASTK family members, particularly FASTKD4 and FASTKD5, are required for non-canonical RNA junction processing of the human mitochondrial transcriptome; loss of FASTKD5 causes severe defects in mitochondrial translation of electron transport chain components and oxidative phosphorylation, and combined knockouts increase specific antisense transcripts.\",\n      \"method\": \"CRISPR/Cas9 knockout of single and combined FASTK family members in human cell lines, comprehensive mitochondrial transcriptome sequencing, mitochondrial translation assays, oxidative phosphorylation measurements\",\n      \"journal\": \"PLoS genetics\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — clean genetic KO with multiple orthogonal functional readouts (transcriptomics, translation, respiration) in human cells\",\n      \"pmids\": [\"34748562\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2013,\n      \"finding\": \"FASTK is a direct target of miR-106a-5p in astrocytoma cells; knockdown of FASTK inhibits cell proliferation and migration and promotes apoptosis, phenocopying miR-106a-5p overexpression, establishing FASTK as a downstream effector mediating these cellular effects.\",\n      \"method\": \"Luciferase reporter assay confirming direct miRNA targeting, western blot, siRNA knockdown with proliferation/migration/apoptosis assays\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 — direct target validation by reporter assay combined with functional loss-of-function phenotype\",\n      \"pmids\": [\"24013584\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"FASTK is a mitochondria-associated serine/threonine kinase that, upon Fas-mediated apoptotic signaling, is dephosphorylated and activated to phosphorylate TIA-1 upstream of DNA fragmentation; it also shuttles to stress granules to modulate translational silencing and to nuclear speckles where it promotes alternative splicing of FGFR2; as the founding member of the FASTK family, it functions as a post-transcriptional regulator of mitochondrial RNA processing, with its homologs collectively governing non-canonical junction processing, ribosome assembly, and translation within mitochondria.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"FASTK is a multifunctional signaling kinase and RNA-processing regulator that operates across mitochondrial, cytoplasmic, and nuclear compartments. Upon Fas receptor ligation, FASTK is dephosphorylated and activated, whereupon it phosphorylates the RNA-binding protein TIA-1 upstream of apoptotic DNA fragmentation [PMID:7544399]. Under basal conditions FASTK localizes to the outer mitochondrial membrane, but upon stress it translocates to cytoplasmic stress granules to modulate translational silencing via TIA-1 interaction, and also resides in nuclear speckles where it promotes FGFR2 exon IIIb inclusion through a U-rich intronic element independently of TIA-1 [PMID:17592127]. As the founding member of the FASTK family, it shares a RAP domain architecture that enables RNA binding and participates in post-transcriptional regulation of mitochondrial gene expression, with family members collectively governing non-canonical RNA junction processing, ribosome assembly, and mitochondrial translation [PMID:29036396, PMID:34748562].\",\n  \"teleology\": [\n    {\n      \"year\": 1995,\n      \"claim\": \"The identification of FASTK as a Fas-activated kinase that phosphorylates TIA-1 established the first direct biochemical link between Fas death-receptor signaling and a downstream kinase–substrate pair operating upstream of DNA fragmentation.\",\n      \"evidence\": \"In vitro kinase assays and immunoprecipitation in Fas-ligated Jurkat cells\",\n      \"pmids\": [\"7544399\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"The structural basis of FASTK kinase activity and its activation by dephosphorylation remains undefined\",\n        \"Whether TIA-1 phosphorylation is necessary and sufficient for downstream DNA fragmentation was not tested\",\n        \"The kinase(s) and phosphatase(s) that regulate FASTK phosphorylation status are unknown\"\n      ]\n    },\n    {\n      \"year\": 2007,\n      \"claim\": \"Demonstration that FASTK resides on the outer mitochondrial membrane yet dynamically relocates to stress granules and nuclear speckles revealed it as a tri-compartmental protein with separable functions in translational silencing (via TIA-1) and alternative splicing (TIA-1-independent promotion of FGFR2 exon IIIb inclusion).\",\n      \"evidence\": \"Two-hybrid screens, co-immunoprecipitation, siRNA knockdown, minigene splicing assays, and immunofluorescence in mammalian cells\",\n      \"pmids\": [\"17592127\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"The signals triggering FASTK redistribution among the three compartments are not characterized\",\n        \"The mechanism by which FASTK recognizes the IAS1 U-rich element to promote exon inclusion is unresolved\",\n        \"Whether the kinase activity of FASTK is required for its splicing or stress-granule functions was not determined\"\n      ]\n    },\n    {\n      \"year\": 2013,\n      \"claim\": \"Validation of FASTK as a direct miR-106a-5p target whose depletion suppresses proliferation and migration while promoting apoptosis in astrocytoma cells placed FASTK in a pro-survival, pro-proliferative role in a cancer context.\",\n      \"evidence\": \"Luciferase reporter assay for direct miRNA targeting, siRNA knockdown with proliferation, migration, and apoptosis assays in astrocytoma cells\",\n      \"pmids\": [\"24013584\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The downstream effectors through which FASTK promotes proliferation and migration were not identified\",\n        \"Findings are from a single cancer cell type without in vivo validation\",\n        \"Whether the kinase activity or RNA-regulatory functions of FASTK mediate these phenotypes is unknown\"\n      ]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"A systematic review consolidated evidence that the FASTK family shares a RAP domain enabling RNA binding and collectively governs mitochondrial mRNA processing, ribosome assembly, and translation, reframing FASTK as the founding member of a mitochondrial RNA-regulatory family.\",\n      \"evidence\": \"Review synthesizing biochemical, genetic, and cell-biology studies across FASTK family members\",\n      \"pmids\": [\"29036396\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The specific mitochondrial RNA targets of FASTK itself (as opposed to its paralogs) were not delineated\",\n        \"No structural data for any FASTK-family RAP domain were available\",\n        \"Whether FASTK and its paralogs function redundantly or in a coordinated manner was not resolved\"\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"CRISPR knockouts demonstrated that FASTK family members, especially FASTKD4 and FASTKD5, are required for non-canonical RNA junction processing of the mitochondrial transcriptome, with FASTKD5 loss causing severe translation and oxidative phosphorylation defects, providing direct genetic evidence for the family's essential role in mitochondrial gene expression.\",\n      \"evidence\": \"Single and combinatorial CRISPR/Cas9 knockouts in human cell lines with mitochondrial transcriptome sequencing, translation assays, and respiration measurements\",\n      \"pmids\": [\"34748562\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"The specific contribution of FASTK itself (versus FASTKD4/D5) to junction processing was not isolated\",\n        \"The mechanism by which individual family members recognize their target RNA junctions is unknown\",\n        \"In vivo physiological consequences of FASTK family loss in animal models have not been reported\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"A key unresolved question is how the kinase activity, RNA-regulatory functions, and dynamic subcellular redistribution of FASTK are coordinated, and which specific mitochondrial RNA targets FASTK itself processes independently of its paralogs.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No structural model of full-length FASTK or its RAP domain exists\",\n        \"The relationship between FASTK kinase activity and its RNA-regulatory roles has never been tested\",\n        \"Specific mitochondrial transcript targets of FASTK (not paralogs) remain to be defined by loss-of-function approaches\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140096\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0003723\", \"supporting_discovery_ids\": [1, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005739\", \"supporting_discovery_ids\": [1, 2, 3]},\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [1]},\n      {\"term_id\": \"GO:0005829\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-5357801\", \"supporting_discovery_ids\": [0, 4]},\n      {\"term_id\": \"R-HSA-8953854\", \"supporting_discovery_ids\": [2, 3]},\n      {\"term_id\": \"R-HSA-74160\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"TIA1\"\n    ],\n    \"other_free_text\": []\n  }\n}\n```"}