{"gene":"EFHB","run_date":"2026-06-09T23:54:42","timeline":{"discoveries":[{"year":2018,"finding":"EFHB (EF-hand domain family member B / CFAP21) was identified as a novel cytosolic Ca2+ sensor that interacts with STIM1 upon ER Ca2+ store depletion and dissociates from STIM1 through a Ca2+-dependent mechanism.","method":"Co-immunoprecipitation followed by Western blotting, fluorescence Ca2+ imaging (fura-2)","journal":"Cellular Physiology and Biochemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal Co-IP with Ca2+-dependent dissociation confirmed, RNAi silencing and overexpression studies in single lab with two orthogonal methods","pmids":["30481768"],"is_preprint":false},{"year":2018,"finding":"EFHB is required for the interaction of STIM1 and Orai1 upon Ca2+ store depletion; RNAi-mediated silencing of EFHB impairs store-operated Ca2+ entry (SOCE) activation.","method":"RNAi knockdown, Ca2+ imaging (fura-2), co-immunoprecipitation","journal":"Cellular Physiology and Biochemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — loss-of-function (RNAi) with defined SOCE phenotype and Co-IP confirmation, single lab","pmids":["30481768"],"is_preprint":false},{"year":2018,"finding":"Silencing EFHB expression abolished the dissociation of SARAF from STIM1, indicating that EFHB modulates the dynamic STIM1-SARAF interaction that is relevant for Orai1 channel activation and subsequent slow Ca2+-dependent inactivation.","method":"RNAi knockdown, co-immunoprecipitation","journal":"Cellular Physiology and Biochemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — functional knockdown phenotype confirmed by Co-IP of STIM1-SARAF complex, single lab","pmids":["30481768"],"is_preprint":false},{"year":2018,"finding":"EFHB overexpression and silencing both affect NFAT translocation from the cytosol to the nucleus, placing EFHB upstream of NFAT-dependent transcriptional responses via SOCE regulation.","method":"RNAi knockdown, overexpression, confocal microscopy of NFAT nuclear translocation","journal":"Cellular Physiology and Biochemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct imaging readout with loss- and gain-of-function, single lab","pmids":["30481768"],"is_preprint":false},{"year":2021,"finding":"EFHB expression is upregulated in luminal and triple-negative breast cancer cells and is essential for full SOCE in these cells; molecular knockdown of EFHB attenuates breast cancer cell proliferation, migration, and viability.","method":"RNAi knockdown, Ca2+ imaging, cell proliferation and migration assays","journal":"Cancers","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — loss-of-function with multiple defined cellular phenotypes (SOCE, proliferation, migration, viability), single lab replicating earlier SOCE findings","pmids":["34439314"],"is_preprint":false}],"current_model":"EFHB is a cytosolic EF-hand domain Ca2+ sensor that interacts with STIM1 upon ER Ca2+ store depletion and dissociates in a Ca2+-dependent manner; it is required for productive STIM1-Orai1 coupling and for the Ca2+-dependent dissociation of the negative regulator SARAF from STIM1, thereby enabling full store-operated Ca2+ entry (SOCE) and downstream NFAT nuclear translocation."},"narrative":{"mechanistic_narrative":"EFHB is a cytosolic EF-hand Ca2+ sensor that gates store-operated Ca2+ entry (SOCE) by controlling the assembly of the STIM1-Orai1 signaling complex following ER Ca2+ store depletion [PMID:30481768]. Upon store depletion EFHB associates with STIM1 and then dissociates in a Ca2+-dependent manner, and this interaction is required for productive STIM1-Orai1 coupling, since RNAi silencing of EFHB impairs SOCE activation [PMID:30481768]. EFHB acts on the regulatory architecture of STIM1 by enabling the dissociation of the negative regulator SARAF from STIM1, the step relevant for Orai1 channel activation and slow Ca2+-dependent inactivation [PMID:30481768]. Through its control of SOCE, EFHB lies upstream of NFAT nuclear translocation and the associated transcriptional responses [PMID:30481768], and its expression is elevated in luminal and triple-negative breast cancer cells where it sustains SOCE and supports proliferation, migration, and viability [PMID:34439314]. Beyond this STIM1/SARAF/SOCE axis, no further mechanistic detail has been characterized in the available corpus.","teleology":[{"year":2018,"claim":"Established EFHB as a previously uncharacterized cytosolic Ca2+ sensor that physically engages STIM1 in a store- and Ca2+-dependent fashion, defining a new node in SOCE regulation.","evidence":"Reciprocal co-immunoprecipitation with Western blotting and fura-2 Ca2+ imaging in cells subjected to ER store depletion","pmids":["30481768"],"confidence":"Medium","gaps":["Direct Ca2+ binding by the EF-hand domains not demonstrated biochemically","Structural basis of the EFHB-STIM1 interaction unknown","Single lab, single cell system"]},{"year":2018,"claim":"Showed EFHB is functionally required for STIM1-Orai1 coupling and SOCE activation, moving it from a binding partner to a necessary effector of the pathway.","evidence":"RNAi knockdown with fura-2 Ca2+ imaging and STIM1-Orai1 co-immunoprecipitation","pmids":["30481768"],"confidence":"Medium","gaps":["Mechanism by which EFHB promotes STIM1-Orai1 coupling not resolved","No rescue with RNAi-resistant construct reported"]},{"year":2018,"claim":"Identified the SARAF-STIM1 dissociation step as the regulatory event controlled by EFHB, linking it to Orai1 activation and slow Ca2+-dependent inactivation.","evidence":"RNAi knockdown with co-immunoprecipitation of the STIM1-SARAF complex","pmids":["30481768"],"confidence":"Medium","gaps":["Whether EFHB acts directly on SARAF or indirectly via STIM1 conformation is unknown","No reconstitution of the EFHB-STIM1-SARAF system"]},{"year":2018,"claim":"Connected EFHB-dependent SOCE to a downstream transcriptional output by showing both loss and gain of EFHB alter NFAT nuclear translocation.","evidence":"RNAi knockdown and overexpression with confocal imaging of NFAT translocation","pmids":["30481768"],"confidence":"Medium","gaps":["Downstream NFAT target genes not defined","Physiological context of the NFAT response not established"]},{"year":2021,"claim":"Placed EFHB-driven SOCE in a disease context by showing its upregulation in breast cancer cells and its requirement for proliferation, migration, and viability.","evidence":"RNAi knockdown with Ca2+ imaging plus proliferation and migration assays in luminal and triple-negative breast cancer cells","pmids":["34439314"],"confidence":"Medium","gaps":["Causal contribution in vivo not tested","Whether the proliferative effect is solely SOCE-dependent is unresolved","Single lab"]},{"year":null,"claim":"How EFHB physically couples Ca2+ sensing to STIM1 conformational change and SARAF release at the molecular and structural level remains unresolved.","evidence":"No structural or reconstituted-system data in the available corpus","pmids":[],"confidence":"Medium","gaps":["No structure of EFHB or its complexes","Direct EF-hand Ca2+ binding affinity unmeasured","Tissue and organismal physiology of EFHB uncharacterized"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0140299","term_label":"molecular sensor activity","supporting_discovery_ids":[0]},{"term_id":"GO:0098772","term_label":"molecular function regulator activity","supporting_discovery_ids":[1,2]}],"localization":[{"term_id":"GO:0005829","term_label":"cytosol","supporting_discovery_ids":[0]}],"pathway":[{"term_id":"R-HSA-162582","term_label":"Signal Transduction","supporting_discovery_ids":[0,1,3]}],"complexes":[],"partners":["STIM1","SARAF","ORAI1"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q8N7U6","full_name":"EF-hand domain-containing family member B","aliases":["Cilia- and flagella-associated protein 21"],"length_aa":833,"mass_kda":93.8,"function":"Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (PubMed:36191189). Cytosolic sensor for calcium, modulates the interaction of STIM1 and ORAI1 upon store depletion and the activation of store-operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to nucleus (PubMed:30481768)","subcellular_location":"Cytoplasm, cytoskeleton, cilium axoneme; Cytoplasm, cytoskeleton, flagellum axoneme; Cytoplasm","url":"https://www.uniprot.org/uniprotkb/Q8N7U6/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/EFHB","classification":"Not Classified","n_dependent_lines":0,"n_total_lines":1208,"dependency_fraction":0.0},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/EFHB","total_profiled":1310},"omim":[],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Cytosol","reliability":"Approved"},{"location":"Nuclear bodies","reliability":"Additional"},{"location":"Vesicles","reliability":"Additional"},{"location":"Connecting piece","reliability":"Additional"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in some","driving_tissues":[{"tissue":"fallopian tube","ntpm":16.0},{"tissue":"testis","ntpm":14.5}],"url":"https://www.proteinatlas.org/search/EFHB"},"hgnc":{"alias_symbol":["FLJ25200","CFAP21"],"prev_symbol":[]},"alphafold":{"accession":"Q8N7U6","domains":[{"cath_id":"1.10.238.10","chopping":"526-647_665-689_703-707","consensus_level":"high","plddt":72.2089,"start":526,"end":707},{"cath_id":"1.10.8","chopping":"771-831","consensus_level":"high","plddt":87.0966,"start":771,"end":831}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q8N7U6","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q8N7U6-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q8N7U6-F1-predicted_aligned_error_v6.png","plddt_mean":62.5},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=EFHB","jax_strain_url":"https://www.jax.org/strain/search?query=EFHB"},"sequence":{"accession":"Q8N7U6","fasta_url":"https://rest.uniprot.org/uniprotkb/Q8N7U6.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q8N7U6/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q8N7U6"}},"corpus_meta":[{"pmid":"19956106","id":"PMC_19956106","title":"Analysis of 19 genes for association with type I diabetes in the Type I Diabetes Genetics Consortium families.","date":"2009","source":"Genes and immunity","url":"https://pubmed.ncbi.nlm.nih.gov/19956106","citation_count":67,"is_preprint":false},{"pmid":"30481768","id":"PMC_30481768","title":"EFHB is a Novel Cytosolic Ca2+ Sensor That Modulates STIM1-SARAF Interaction.","date":"2018","source":"Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology","url":"https://pubmed.ncbi.nlm.nih.gov/30481768","citation_count":32,"is_preprint":false},{"pmid":"34439314","id":"PMC_34439314","title":"SARAF and EFHB Modulate Store-Operated Ca2+ Entry and Are Required for Cell Proliferation, Migration and Viability in Breast Cancer Cells.","date":"2021","source":"Cancers","url":"https://pubmed.ncbi.nlm.nih.gov/34439314","citation_count":13,"is_preprint":false},{"pmid":"30696947","id":"PMC_30696947","title":"A germline HLTF mutation in familial MDS induces DNA damage accumulation through impaired PCNA polyubiquitination.","date":"2019","source":"Leukemia","url":"https://pubmed.ncbi.nlm.nih.gov/30696947","citation_count":13,"is_preprint":false},{"pmid":"36330447","id":"PMC_36330447","title":"Integrated analysis of the whole transcriptome of skeletal muscle reveals the ceRNA regulatory network related to the formation of muscle fibers in Tan sheep.","date":"2022","source":"Frontiers in genetics","url":"https://pubmed.ncbi.nlm.nih.gov/36330447","citation_count":11,"is_preprint":false},{"pmid":"40282783","id":"PMC_40282783","title":"Transcriptome-Metabolome Analysis Reveals That Crossbreeding Improves Meat Quality in Hu Sheep and Their F1-Generation Sheep.","date":"2025","source":"Foods (Basel, Switzerland)","url":"https://pubmed.ncbi.nlm.nih.gov/40282783","citation_count":8,"is_preprint":false},{"pmid":"30921660","id":"PMC_30921660","title":"Synthesis of (E)-Ethyl-4-(2-(furan-2-ylmethylene)hydrazinyl)benzoate, crystal structure, and studies of its interactions with human serum albumin by spectroscopic fluorescence and molecular docking methods.","date":"2019","source":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","url":"https://pubmed.ncbi.nlm.nih.gov/30921660","citation_count":4,"is_preprint":false},{"pmid":"41374450","id":"PMC_41374450","title":"Exome-Based Identification of Candidate Genes in Sporadic Adenomyosis Cases.","date":"2025","source":"Diagnostics (Basel, Switzerland)","url":"https://pubmed.ncbi.nlm.nih.gov/41374450","citation_count":0,"is_preprint":false},{"pmid":"41257575","id":"PMC_41257575","title":"Genetic analysis and quantitative trait loci detection of udder traits in Jersey cattle.","date":"2025","source":"BMC genomics","url":"https://pubmed.ncbi.nlm.nih.gov/41257575","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":7450,"output_tokens":1128,"usd":0.019635,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7700,"output_tokens":2016,"usd":0.04445,"stage2_stop_reason":"end_turn"},"total_usd":0.064085,"stage1_batch_id":"msgbatch_01AsR7g8dGTEiEw9U83bJeWn","stage2_batch_id":"msgbatch_01YV5ZyxtFNUZEjxRcpd9jzb","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2018,\n      \"finding\": \"EFHB (EF-hand domain family member B / CFAP21) was identified as a novel cytosolic Ca2+ sensor that interacts with STIM1 upon ER Ca2+ store depletion and dissociates from STIM1 through a Ca2+-dependent mechanism.\",\n      \"method\": \"Co-immunoprecipitation followed by Western blotting, fluorescence Ca2+ imaging (fura-2)\",\n      \"journal\": \"Cellular Physiology and Biochemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal Co-IP with Ca2+-dependent dissociation confirmed, RNAi silencing and overexpression studies in single lab with two orthogonal methods\",\n      \"pmids\": [\"30481768\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"EFHB is required for the interaction of STIM1 and Orai1 upon Ca2+ store depletion; RNAi-mediated silencing of EFHB impairs store-operated Ca2+ entry (SOCE) activation.\",\n      \"method\": \"RNAi knockdown, Ca2+ imaging (fura-2), co-immunoprecipitation\",\n      \"journal\": \"Cellular Physiology and Biochemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — loss-of-function (RNAi) with defined SOCE phenotype and Co-IP confirmation, single lab\",\n      \"pmids\": [\"30481768\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"Silencing EFHB expression abolished the dissociation of SARAF from STIM1, indicating that EFHB modulates the dynamic STIM1-SARAF interaction that is relevant for Orai1 channel activation and subsequent slow Ca2+-dependent inactivation.\",\n      \"method\": \"RNAi knockdown, co-immunoprecipitation\",\n      \"journal\": \"Cellular Physiology and Biochemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — functional knockdown phenotype confirmed by Co-IP of STIM1-SARAF complex, single lab\",\n      \"pmids\": [\"30481768\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"EFHB overexpression and silencing both affect NFAT translocation from the cytosol to the nucleus, placing EFHB upstream of NFAT-dependent transcriptional responses via SOCE regulation.\",\n      \"method\": \"RNAi knockdown, overexpression, confocal microscopy of NFAT nuclear translocation\",\n      \"journal\": \"Cellular Physiology and Biochemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct imaging readout with loss- and gain-of-function, single lab\",\n      \"pmids\": [\"30481768\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"EFHB expression is upregulated in luminal and triple-negative breast cancer cells and is essential for full SOCE in these cells; molecular knockdown of EFHB attenuates breast cancer cell proliferation, migration, and viability.\",\n      \"method\": \"RNAi knockdown, Ca2+ imaging, cell proliferation and migration assays\",\n      \"journal\": \"Cancers\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — loss-of-function with multiple defined cellular phenotypes (SOCE, proliferation, migration, viability), single lab replicating earlier SOCE findings\",\n      \"pmids\": [\"34439314\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"EFHB is a cytosolic EF-hand domain Ca2+ sensor that interacts with STIM1 upon ER Ca2+ store depletion and dissociates in a Ca2+-dependent manner; it is required for productive STIM1-Orai1 coupling and for the Ca2+-dependent dissociation of the negative regulator SARAF from STIM1, thereby enabling full store-operated Ca2+ entry (SOCE) and downstream NFAT nuclear translocation.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"EFHB is a cytosolic EF-hand Ca2+ sensor that gates store-operated Ca2+ entry (SOCE) by controlling the assembly of the STIM1-Orai1 signaling complex following ER Ca2+ store depletion [#0, #1]. Upon store depletion EFHB associates with STIM1 and then dissociates in a Ca2+-dependent manner, and this interaction is required for productive STIM1-Orai1 coupling, since RNAi silencing of EFHB impairs SOCE activation [#0, #1]. EFHB acts on the regulatory architecture of STIM1 by enabling the dissociation of the negative regulator SARAF from STIM1, the step relevant for Orai1 channel activation and slow Ca2+-dependent inactivation [#2]. Through its control of SOCE, EFHB lies upstream of NFAT nuclear translocation and the associated transcriptional responses [#3], and its expression is elevated in luminal and triple-negative breast cancer cells where it sustains SOCE and supports proliferation, migration, and viability [#4]. Beyond this STIM1/SARAF/SOCE axis, no further mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2018,\n      \"claim\": \"Established EFHB as a previously uncharacterized cytosolic Ca2+ sensor that physically engages STIM1 in a store- and Ca2+-dependent fashion, defining a new node in SOCE regulation.\",\n      \"evidence\": \"Reciprocal co-immunoprecipitation with Western blotting and fura-2 Ca2+ imaging in cells subjected to ER store depletion\",\n      \"pmids\": [\"30481768\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct Ca2+ binding by the EF-hand domains not demonstrated biochemically\",\n        \"Structural basis of the EFHB-STIM1 interaction unknown\",\n        \"Single lab, single cell system\"\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Showed EFHB is functionally required for STIM1-Orai1 coupling and SOCE activation, moving it from a binding partner to a necessary effector of the pathway.\",\n      \"evidence\": \"RNAi knockdown with fura-2 Ca2+ imaging and STIM1-Orai1 co-immunoprecipitation\",\n      \"pmids\": [\"30481768\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Mechanism by which EFHB promotes STIM1-Orai1 coupling not resolved\",\n        \"No rescue with RNAi-resistant construct reported\"\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Identified the SARAF-STIM1 dissociation step as the regulatory event controlled by EFHB, linking it to Orai1 activation and slow Ca2+-dependent inactivation.\",\n      \"evidence\": \"RNAi knockdown with co-immunoprecipitation of the STIM1-SARAF complex\",\n      \"pmids\": [\"30481768\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Whether EFHB acts directly on SARAF or indirectly via STIM1 conformation is unknown\",\n        \"No reconstitution of the EFHB-STIM1-SARAF system\"\n      ]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Connected EFHB-dependent SOCE to a downstream transcriptional output by showing both loss and gain of EFHB alter NFAT nuclear translocation.\",\n      \"evidence\": \"RNAi knockdown and overexpression with confocal imaging of NFAT translocation\",\n      \"pmids\": [\"30481768\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Downstream NFAT target genes not defined\",\n        \"Physiological context of the NFAT response not established\"\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"Placed EFHB-driven SOCE in a disease context by showing its upregulation in breast cancer cells and its requirement for proliferation, migration, and viability.\",\n      \"evidence\": \"RNAi knockdown with Ca2+ imaging plus proliferation and migration assays in luminal and triple-negative breast cancer cells\",\n      \"pmids\": [\"34439314\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Causal contribution in vivo not tested\",\n        \"Whether the proliferative effect is solely SOCE-dependent is unresolved\",\n        \"Single lab\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How EFHB physically couples Ca2+ sensing to STIM1 conformational change and SARAF release at the molecular and structural level remains unresolved.\",\n      \"evidence\": \"No structural or reconstituted-system data in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No structure of EFHB or its complexes\",\n        \"Direct EF-hand Ca2+ binding affinity unmeasured\",\n        \"Tissue and organismal physiology of EFHB uncharacterized\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140299\", \"supporting_discovery_ids\": [0]},\n      {\"term_id\": \"GO:0098772\", \"supporting_discovery_ids\": [1, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005829\", \"supporting_discovery_ids\": [0]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-162582\", \"supporting_discovery_ids\": [0, 1, 3]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"STIM1\", \"SARAF\", \"ORAI1\"],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":4,"faith_total":4,"faith_pct":100.0}}