{"gene":"CIB3","run_date":"2026-04-28T17:28:52","timeline":{"discoveries":[{"year":2021,"finding":"CIB3 is an auxiliary subunit of the mechanotransduction (MET) channel in inner-ear hair cells, binding to TMC1/2 through a domain flanked by transmembrane domains 2 and 3; the co-crystal structure of the CIB-binding domain of TMC1 with CIB3 reveals interactions mediated through a conserved CIB hydrophobic groove, structurally similar to KChIP1 binding of Kv4 channels.","method":"X-ray crystallography, co-crystal structure, functional studies in mice (KO), binding assays","journal":"Neuron","confidence":"High","confidence_rationale":"Tier 1 — crystal structure with functional validation in knockout mice, multiple orthogonal methods","pmids":["34089643"],"is_preprint":false},{"year":2021,"finding":"CIB3 can functionally substitute for CIB2 in cochlear hair cells to support mechanoelectrical transduction, and CIB2 regulates TMC1/2 localization and function; deafness-causing CIB2 mutations affect these processes.","method":"Functional studies in CIB2/CIB3 knockout mice, electrophysiology (MET current recordings), immunolocalization","journal":"Neuron","confidence":"High","confidence_rationale":"Tier 2 — genetic KO with defined electrophysiological phenotype, replicated across CIB2/CIB3 conditions","pmids":["34089643"],"is_preprint":false},{"year":2023,"finding":"CIB2 and CIB3 act redundantly to regulate MET currents in vestibular hair cells (VHCs); Cib2/Cib3 double knockout completely abolishes MET currents in VHCs and leads to severe balance deficits, while single knockouts show region-specific stereocilia maintenance defects (CIB2 in striolar, CIB3 in extrastriolar VHCs).","method":"Double and single knockout mice, electrophysiology (MET currents), fluorescence microscopy of stereocilia","journal":"The Journal of neuroscience","confidence":"High","confidence_rationale":"Tier 2 — double KO with defined electrophysiological and morphological phenotypes, complementary expression patterns confirmed","pmids":["37001993"],"is_preprint":false},{"year":2024,"finding":"CIB3 localizes to microvillar tips in intestinal epithelial cells near glycocalyx insertion sites and forms a complex with TMC5 (a fragment of TMC5 binds CIB3 in vitro), suggesting CIB3 participates in a mechanosensitive complex at microvillar tips analogous to its role in inner ear stereocilia.","method":"Colocalization (fluorescence microscopy), in vitro complex formation (pulldown of TMC5 fragment with CIB3)","journal":"bioRxiv","confidence":"Low","confidence_rationale":"Tier 3 — single pulldown with fragment, preprint, no functional KO validation","pmids":["bio_10.1101_2024.08.22.609173"],"is_preprint":true}],"current_model":"CIB3 is an auxiliary subunit of the mechanotransduction (MET) channel in inner-ear hair cells, binding directly to TMC1/2 through a conserved hydrophobic groove (structurally analogous to KChIP auxiliary subunits of Kv4 channels), where it redundantly supports MET current generation and stereocilia maintenance alongside CIB2, with recent evidence suggesting it may also associate with TMC5 at intestinal microvillar tips."},"narrative":{"teleology":[{"year":2021,"claim":"Determining how CIB proteins physically engage the MET channel complex, co-crystallization of CIB3 with the TMC1 CIB-binding domain revealed that CIB3 binds TMC1/2 via a conserved hydrophobic groove between transmembrane domains 2 and 3, structurally analogous to KChIP auxiliary subunit binding of Kv4 channels, establishing CIB3 as a bona fide auxiliary subunit of the MET channel.","evidence":"X-ray co-crystal structure of CIB3–TMC1 CIB-binding domain, binding assays, and functional validation in knockout mice","pmids":["34089643"],"confidence":"High","gaps":["Full-length TMC1/2–CIB3 complex structure not resolved","Whether CIB3 modulates channel gating kinetics or solely stabilizes TMC localization is not distinguished","Calcium-dependence of CIB3–TMC interaction not fully characterized"]},{"year":2021,"claim":"Establishing functional redundancy with CIB2, experiments showed that CIB3 can substitute for CIB2 in supporting cochlear MET currents and that deafness-causing CIB2 mutations disrupt TMC1/2 localization, demonstrating that CIB family members are interchangeable in maintaining hair-cell mechanotransduction.","evidence":"Electrophysiological MET current recordings and immunolocalization in CIB2/CIB3 knockout mouse cochlear hair cells","pmids":["34089643"],"confidence":"High","gaps":["Whether CIB3 loss alone causes any auditory phenotype in vivo is not resolved","Quantitative differences in MET current properties between CIB2- and CIB3-supported channels not characterized"]},{"year":2023,"claim":"Extending the redundancy model to the vestibular system, double knockout of Cib2 and Cib3 completely abolished MET currents in vestibular hair cells and caused severe balance deficits, while single knockouts revealed region-specific roles—CIB2 in striolar and CIB3 in extrastriolar vestibular hair cells—for stereocilia maintenance.","evidence":"Electrophysiology and fluorescence microscopy of stereocilia in single and double Cib2/Cib3 knockout mice","pmids":["37001993"],"confidence":"High","gaps":["Molecular basis for differential expression of CIB2 versus CIB3 across vestibular regions unknown","Whether CIB3 has non-redundant functions in extrastriolar hair cells beyond stereocilia maintenance not tested"]},{"year":2024,"claim":"A preprint reported CIB3 localization at intestinal microvillar tips and its in vitro association with a TMC5 fragment, raising the possibility that CIB3 participates in a mechanosensitive complex outside the inner ear.","evidence":"(preprint) Fluorescence microscopy colocalization and pulldown of TMC5 fragment with CIB3 in intestinal epithelial cells","pmids":["bio_10.1101_2024.08.22.609173"],"confidence":"Low","gaps":["Single pulldown with a fragment, no full-length TMC5–CIB3 binding demonstrated","No functional knockout validation of CIB3 in intestinal epithelium","Mechanosensitive function at microvillar tips not tested"]},{"year":null,"claim":"It remains unknown whether CIB3 directly modulates MET channel gating kinetics versus solely stabilizing TMC localization, what determines its region-specific expression pattern in the vestibular system, and whether the intestinal TMC5–CIB3 interaction is physiologically relevant.","evidence":"","pmids":[],"confidence":"Low","gaps":["No reconstituted MET channel system to dissect CIB3 gating effects","Transcriptional regulation of CIB3 expression not characterized","Full-length TMC5–CIB3 complex formation and in vivo intestinal function unresolved"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0098772","term_label":"molecular function regulator activity","supporting_discovery_ids":[0,1,2]}],"localization":[{"term_id":"GO:0005929","term_label":"cilium","supporting_discovery_ids":[0,1,2]}],"pathway":[{"term_id":"R-HSA-9709957","term_label":"Sensory Perception","supporting_discovery_ids":[0,1,2]}],"complexes":[],"partners":["TMC1","TMC2","CIB2"],"other_free_text":[]},"mechanistic_narrative":"CIB3 is an auxiliary subunit of the mechanotransduction (MET) channel in inner-ear hair cells that binds directly to TMC1 and TMC2 through a conserved hydrophobic groove situated between transmembrane domains 2 and 3, as revealed by co-crystal structure analysis showing structural similarity to KChIP1 binding of Kv4 channels [PMID:34089643]. CIB3 can functionally substitute for CIB2 in cochlear hair cells to support mechanoelectrical transduction, and in vestibular hair cells CIB2 and CIB3 act redundantly such that double knockout completely abolishes MET currents and causes severe balance deficits, while single knockouts produce region-specific stereocilia maintenance defects [PMID:34089643, PMID:37001993]."},"prefetch_data":{"uniprot":{"accession":"Q96Q77","full_name":"Calcium and integrin-binding family member 3","aliases":["Kinase-interacting protein 3","KIP 3"],"length_aa":187,"mass_kda":21.8,"function":"Acts a an auxiliary subunit of the sensory mechanoelectrical transduction (MET) channel in hair cells (By similarity). Plays a role in regulating hair cell MET channel localization and function (By similarity)","subcellular_location":"","url":"https://www.uniprot.org/uniprotkb/Q96Q77/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/CIB3","classification":"Not Classified","n_dependent_lines":0,"n_total_lines":1208,"dependency_fraction":0.0},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/CIB3","total_profiled":1310},"omim":[{"mim_id":"610645","title":"CALCIUM- AND INTEGRIN-BINDING PROTEIN 3; CIB3","url":"https://www.omim.org/entry/610645"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Plasma membrane","reliability":"Approved"},{"location":"Cytosol","reliability":"Approved"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in single","driving_tissues":[{"tissue":"bone marrow","ntpm":1.2}],"url":"https://www.proteinatlas.org/search/CIB3"},"hgnc":{"alias_symbol":["KIP3"],"prev_symbol":[]},"alphafold":{"accession":"Q96Q77","domains":[{"cath_id":"1.10.238.10","chopping":"11-99","consensus_level":"high","plddt":92.2157,"start":11,"end":99},{"cath_id":"1.10.238.10","chopping":"105-183","consensus_level":"high","plddt":87.9924,"start":105,"end":183}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q96Q77","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q96Q77-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q96Q77-F1-predicted_aligned_error_v6.png","plddt_mean":85.06},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=CIB3","jax_strain_url":"https://www.jax.org/strain/search?query=CIB3"},"sequence":{"accession":"Q96Q77","fasta_url":"https://rest.uniprot.org/uniprotkb/Q96Q77.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q96Q77/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q96Q77"}},"corpus_meta":[{"pmid":"16906148","id":"PMC_16906148","title":"Plus end-specific depolymerase activity of Kip3, a kinesin-8 protein, explains its role in positioning the yeast mitotic spindle.","date":"2006","source":"Nature cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/16906148","citation_count":263,"is_preprint":false},{"pmid":"9281581","id":"PMC_9281581","title":"Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration.","date":"1997","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/9281581","citation_count":154,"is_preprint":false},{"pmid":"34089643","id":"PMC_34089643","title":"CIB2 and CIB3 are auxiliary subunits of the mechanotransduction channel of hair cells.","date":"2021","source":"Neuron","url":"https://pubmed.ncbi.nlm.nih.gov/34089643","citation_count":63,"is_preprint":false},{"pmid":"20603597","id":"PMC_20603597","title":"Kip3, the yeast kinesin-8, is required for clustering of kinetochores at metaphase.","date":"2010","source":"Cell cycle (Georgetown, Tex.)","url":"https://pubmed.ncbi.nlm.nih.gov/20603597","citation_count":57,"is_preprint":false},{"pmid":"15189985","id":"PMC_15189985","title":"The Kip3-like kinesin KipB moves along microtubules and determines spindle position during synchronized mitoses in Aspergillus nidulans hyphae.","date":"2004","source":"Eukaryotic cell","url":"https://pubmed.ncbi.nlm.nih.gov/15189985","citation_count":44,"is_preprint":false},{"pmid":"24616221","id":"PMC_24616221","title":"The kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization.","date":"2014","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/24616221","citation_count":36,"is_preprint":false},{"pmid":"37001993","id":"PMC_37001993","title":"CIB2 and CIB3 Regulate Stereocilia Maintenance and Mechanoelectrical Transduction in Mouse Vestibular Hair Cells.","date":"2023","source":"The Journal of neuroscience : the official journal of the Society for Neuroscience","url":"https://pubmed.ncbi.nlm.nih.gov/37001993","citation_count":17,"is_preprint":false},{"pmid":"30360926","id":"PMC_30360926","title":"Three-Dimensional Optical Tweezers Tracking Resolves Random Sideward Steps of the Kinesin-8 Kip3.","date":"2018","source":"Biophysical journal","url":"https://pubmed.ncbi.nlm.nih.gov/30360926","citation_count":13,"is_preprint":false},{"pmid":"32229316","id":"PMC_32229316","title":"The Kinesin-8 Kip3 Depolymerizes Microtubules with a Collective Force-Dependent Mechanism.","date":"2020","source":"Biophysical journal","url":"https://pubmed.ncbi.nlm.nih.gov/32229316","citation_count":12,"is_preprint":false},{"pmid":"33686404","id":"PMC_33686404","title":"Arabidopsis CIB3 regulates photoperiodic flowering in an FKF1-dependent way.","date":"2021","source":"Bioscience, biotechnology, and biochemistry","url":"https://pubmed.ncbi.nlm.nih.gov/33686404","citation_count":10,"is_preprint":false},{"pmid":"29874146","id":"PMC_29874146","title":"Discrete regions of the kinesin-8 Kip3 tail differentially mediate astral microtubule stability and spindle disassembly.","date":"2018","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/29874146","citation_count":7,"is_preprint":false},{"pmid":null,"id":"bio_10.1101_2025.01.27.635192","title":"Kinesin-8/Kip3 requires beta tubulin tail for depolymerase activity","date":"2025-01-29","source":"bioRxiv","url":"https://doi.org/10.1101/2025.01.27.635192","citation_count":0,"is_preprint":true},{"pmid":null,"id":"bio_10.1101_2024.08.22.609173","title":"Transmembrane channel-like 4 and 5 proteins at microvillar tips are potential ion channels and lipid scramblases","date":"2024-08-23","source":"bioRxiv","url":"https://doi.org/10.1101/2024.08.22.609173","citation_count":0,"is_preprint":true},{"pmid":null,"id":"bio_10.1101_2024.05.24.595729","title":"Molecular Specializations Underlying Phenotypic Differences in Inner Ear Hair Cells of Zebrafish and Mice","date":"2024-05-26","source":"bioRxiv","url":"https://doi.org/10.1101/2024.05.24.595729","citation_count":0,"is_preprint":true},{"pmid":null,"id":"bio_10.1101_2024.09.05.611543","title":"Kinesin-8 motors dimerize by folding their proximal tail domain into a compact helical bundle","date":"2024-09-05","source":"bioRxiv","url":"https://doi.org/10.1101/2024.09.05.611543","citation_count":0,"is_preprint":true}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":8607,"output_tokens":1024,"usd":0.020591},"stage2":{"model":"claude-opus-4-6","input_tokens":4223,"output_tokens":1526,"usd":0.088898},"total_usd":0.109489,"stage1_batch_id":"msgbatch_011kfMfE81o6CTcyhVUqumuW","stage2_batch_id":"msgbatch_01DTputS6WNkH745x1nVSoBo","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2021,\n      \"finding\": \"CIB3 is an auxiliary subunit of the mechanotransduction (MET) channel in inner-ear hair cells, binding to TMC1/2 through a domain flanked by transmembrane domains 2 and 3; the co-crystal structure of the CIB-binding domain of TMC1 with CIB3 reveals interactions mediated through a conserved CIB hydrophobic groove, structurally similar to KChIP1 binding of Kv4 channels.\",\n      \"method\": \"X-ray crystallography, co-crystal structure, functional studies in mice (KO), binding assays\",\n      \"journal\": \"Neuron\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 — crystal structure with functional validation in knockout mice, multiple orthogonal methods\",\n      \"pmids\": [\"34089643\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"CIB3 can functionally substitute for CIB2 in cochlear hair cells to support mechanoelectrical transduction, and CIB2 regulates TMC1/2 localization and function; deafness-causing CIB2 mutations affect these processes.\",\n      \"method\": \"Functional studies in CIB2/CIB3 knockout mice, electrophysiology (MET current recordings), immunolocalization\",\n      \"journal\": \"Neuron\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — genetic KO with defined electrophysiological phenotype, replicated across CIB2/CIB3 conditions\",\n      \"pmids\": [\"34089643\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"CIB2 and CIB3 act redundantly to regulate MET currents in vestibular hair cells (VHCs); Cib2/Cib3 double knockout completely abolishes MET currents in VHCs and leads to severe balance deficits, while single knockouts show region-specific stereocilia maintenance defects (CIB2 in striolar, CIB3 in extrastriolar VHCs).\",\n      \"method\": \"Double and single knockout mice, electrophysiology (MET currents), fluorescence microscopy of stereocilia\",\n      \"journal\": \"The Journal of neuroscience\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 — double KO with defined electrophysiological and morphological phenotypes, complementary expression patterns confirmed\",\n      \"pmids\": [\"37001993\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"CIB3 localizes to microvillar tips in intestinal epithelial cells near glycocalyx insertion sites and forms a complex with TMC5 (a fragment of TMC5 binds CIB3 in vitro), suggesting CIB3 participates in a mechanosensitive complex at microvillar tips analogous to its role in inner ear stereocilia.\",\n      \"method\": \"Colocalization (fluorescence microscopy), in vitro complex formation (pulldown of TMC5 fragment with CIB3)\",\n      \"journal\": \"bioRxiv\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 — single pulldown with fragment, preprint, no functional KO validation\",\n      \"pmids\": [\"bio_10.1101_2024.08.22.609173\"],\n      \"is_preprint\": true\n    }\n  ],\n  \"current_model\": \"CIB3 is an auxiliary subunit of the mechanotransduction (MET) channel in inner-ear hair cells, binding directly to TMC1/2 through a conserved hydrophobic groove (structurally analogous to KChIP auxiliary subunits of Kv4 channels), where it redundantly supports MET current generation and stereocilia maintenance alongside CIB2, with recent evidence suggesting it may also associate with TMC5 at intestinal microvillar tips.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"CIB3 is an auxiliary subunit of the mechanotransduction (MET) channel in inner-ear hair cells that binds directly to TMC1 and TMC2 through a conserved hydrophobic groove situated between transmembrane domains 2 and 3, as revealed by co-crystal structure analysis showing structural similarity to KChIP1 binding of Kv4 channels [PMID:34089643]. CIB3 can functionally substitute for CIB2 in cochlear hair cells to support mechanoelectrical transduction, and in vestibular hair cells CIB2 and CIB3 act redundantly such that double knockout completely abolishes MET currents and causes severe balance deficits, while single knockouts produce region-specific stereocilia maintenance defects [PMID:34089643, PMID:37001993].\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"Determining how CIB proteins physically engage the MET channel complex, co-crystallization of CIB3 with the TMC1 CIB-binding domain revealed that CIB3 binds TMC1/2 via a conserved hydrophobic groove between transmembrane domains 2 and 3, structurally analogous to KChIP auxiliary subunit binding of Kv4 channels, establishing CIB3 as a bona fide auxiliary subunit of the MET channel.\",\n      \"evidence\": \"X-ray co-crystal structure of CIB3–TMC1 CIB-binding domain, binding assays, and functional validation in knockout mice\",\n      \"pmids\": [\"34089643\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Full-length TMC1/2–CIB3 complex structure not resolved\",\n        \"Whether CIB3 modulates channel gating kinetics or solely stabilizes TMC localization is not distinguished\",\n        \"Calcium-dependence of CIB3–TMC interaction not fully characterized\"\n      ]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"Establishing functional redundancy with CIB2, experiments showed that CIB3 can substitute for CIB2 in supporting cochlear MET currents and that deafness-causing CIB2 mutations disrupt TMC1/2 localization, demonstrating that CIB family members are interchangeable in maintaining hair-cell mechanotransduction.\",\n      \"evidence\": \"Electrophysiological MET current recordings and immunolocalization in CIB2/CIB3 knockout mouse cochlear hair cells\",\n      \"pmids\": [\"34089643\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Whether CIB3 loss alone causes any auditory phenotype in vivo is not resolved\",\n        \"Quantitative differences in MET current properties between CIB2- and CIB3-supported channels not characterized\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Extending the redundancy model to the vestibular system, double knockout of Cib2 and Cib3 completely abolished MET currents in vestibular hair cells and caused severe balance deficits, while single knockouts revealed region-specific roles—CIB2 in striolar and CIB3 in extrastriolar vestibular hair cells—for stereocilia maintenance.\",\n      \"evidence\": \"Electrophysiology and fluorescence microscopy of stereocilia in single and double Cib2/Cib3 knockout mice\",\n      \"pmids\": [\"37001993\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Molecular basis for differential expression of CIB2 versus CIB3 across vestibular regions unknown\",\n        \"Whether CIB3 has non-redundant functions in extrastriolar hair cells beyond stereocilia maintenance not tested\"\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"A preprint reported CIB3 localization at intestinal microvillar tips and its in vitro association with a TMC5 fragment, raising the possibility that CIB3 participates in a mechanosensitive complex outside the inner ear.\",\n      \"evidence\": \"(preprint) Fluorescence microscopy colocalization and pulldown of TMC5 fragment with CIB3 in intestinal epithelial cells\",\n      \"pmids\": [\"bio_10.1101_2024.08.22.609173\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single pulldown with a fragment, no full-length TMC5–CIB3 binding demonstrated\",\n        \"No functional knockout validation of CIB3 in intestinal epithelium\",\n        \"Mechanosensitive function at microvillar tips not tested\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown whether CIB3 directly modulates MET channel gating kinetics versus solely stabilizing TMC localization, what determines its region-specific expression pattern in the vestibular system, and whether the intestinal TMC5–CIB3 interaction is physiologically relevant.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No reconstituted MET channel system to dissect CIB3 gating effects\",\n        \"Transcriptional regulation of CIB3 expression not characterized\",\n        \"Full-length TMC5–CIB3 complex formation and in vivo intestinal function unresolved\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0098772\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005929\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-9709957\", \"supporting_discovery_ids\": [0, 1, 2]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"TMC1\", \"TMC2\", \"CIB2\"],\n    \"other_free_text\": []\n  }\n}\n```"}