{"gene":"CGN","run_date":"2026-06-09T22:57:18","timeline":{"discoveries":[{"year":2023,"finding":"Knockout of CGN (cingulin) and/or CGNL1 (paracingulin) in MDCK kidney epithelial cells causes a modest but significant increase in transepithelial resistance and decreased junctional accumulation of claudin-2, with reduced claudin-2 protein levels; this phenotype was rescued by CGN or CGNL1 overexpression but not by ZO-1 overexpression, establishing that CGN regulates claudin-2 junctional localization and expression independently of ZO-1.","method":"CRISPR knockout in MDCK cells, calcium switch assay, transepithelial resistance measurement, dextran permeability assay, rescue by overexpression","journal":"Cells","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean KO with defined cellular phenotype and rescue experiment, single lab with multiple orthogonal methods","pmids":["37566083"],"is_preprint":false},{"year":2023,"finding":"CGN (cingulin) plays a role in tethering ZO-1 to the actomyosin and microtubule cytoskeletons at tight junctions, and is dispensable for overall epithelial barrier function in MDCK cells.","method":"Knockout in MDCK cells, transepithelial resistance measurement, permeability assays","journal":"Cells","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — KO phenotype with functional barrier readouts, single lab","pmids":["37566083"],"is_preprint":false},{"year":2021,"finding":"CGN (cingulin) is a direct target of miR-125b in colorectal cancer cells; miR-125b suppresses CGN expression via its 3'UTR (validated by dual-luciferase reporter assay), and CGN loss activates the RhoA/ROCK pathway to promote cell migration and invasion.","method":"Dual-luciferase reporter assay, RNA-seq, overexpression/knockdown of miR-125b and CGN in CRC cells, in vitro migration/invasion assays, in vivo xenograft","journal":"Cancers","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct 3'UTR luciferase validation plus functional in vitro and in vivo assays, single lab with multiple orthogonal methods","pmids":["34830864"],"is_preprint":false},{"year":2025,"finding":"CGN (cingulin) upregulation by polyphyllin H blocks the RhoA/Rock2 signaling pathway in ovarian cancer cells, inhibiting proliferation, invasion, and migration; protein sequencing identified CGN as the mechanistic target mediating polyphyllin H's anti-tumor effects.","method":"CCK-8 proliferation assay, in vitro invasion/migration assays, CDX and PDX mouse models, protein sequencing/proteomics, in vivo tumor growth measurement","journal":"International journal of surgery (London, England)","confidence":"Low","confidence_rationale":"Tier 3 / Weak — protein sequencing-based pathway inference in a single study; mechanistic link between CGN and RhoA/Rock2 not directly validated by mutagenesis or reconstitution","pmids":["40773225"],"is_preprint":false}],"current_model":"Cingulin (CGN) is a cytoplasmic tight junction scaffold protein that tethers ZO-1 to the actomyosin and microtubule cytoskeletons, regulates claudin-2 junctional localization and expression independently of ZO-1, and suppresses cell migration and invasion by restraining the RhoA/ROCK signaling pathway; it is dispensable for overall epithelial paracellular barrier function."},"narrative":{"mechanistic_narrative":"Cingulin (CGN) is a cytoplasmic tight junction scaffold that links the junctional adaptor ZO-1 to the actomyosin and microtubule cytoskeletons in epithelial cells [PMID:37566083]. It controls the junctional accumulation and protein abundance of claudin-2, and this regulation is independent of ZO-1, as CGN or its relative CGNL1 — but not ZO-1 — rescue the loss-of-CGN phenotype [PMID:37566083]. CGN is dispensable for overall epithelial paracellular barrier function, with its loss producing only a modest increase in transepithelial resistance [PMID:37566083]. Beyond its junctional role, CGN restrains the RhoA/ROCK signaling pathway: loss of CGN activates RhoA/ROCK to promote cell migration and invasion, and restoring CGN suppresses these behaviors in cancer cells [PMID:34830864].","teleology":[{"year":2021,"claim":"Established that CGN is a post-transcriptionally regulated suppressor of cell motility, defining its link to RhoA/ROCK signaling in cancer.","evidence":"dual-luciferase 3'UTR reporter, miR-125b/CGN overexpression and knockdown, migration/invasion assays and xenografts in colorectal cancer cells","pmids":["34830864"],"confidence":"Medium","gaps":["the molecular step by which CGN restrains RhoA is not defined (no GEF/GAP or direct binding partner identified)","link is correlative for the signaling axis rather than reconstituted","generality beyond colorectal cancer untested at this point"]},{"year":2023,"claim":"Defined CGN's core junctional mechanism by separating its cytoskeletal-tethering and claudin-2-regulatory functions from barrier maintenance and from ZO-1.","evidence":"CRISPR knockout of CGN/CGNL1 in MDCK cells with calcium switch, transepithelial resistance, dextran permeability, and overexpression rescue","pmids":["37566083"],"confidence":"Medium","gaps":["mechanism by which CGN controls claudin-2 protein levels (transcriptional vs stability) unresolved","single cell line (MDCK) and single lab","direct binding interfaces to ZO-1, actomyosin, and microtubules not mapped here"]},{"year":2025,"claim":"Extended the CGN–RhoA/ROCK suppressor axis to ovarian cancer and nominated CGN as a druggable target of a natural compound.","evidence":"polyphyllin H treatment with proteomics/protein sequencing, proliferation/invasion/migration assays, CDX and PDX models","pmids":["40773225"],"confidence":"Low","gaps":["CGN-RhoA/Rock2 link inferred from protein sequencing, not validated by mutagenesis or reconstitution","whether polyphyllin H acts directly or indirectly on CGN is unknown","not independently confirmed"]},{"year":null,"claim":"How CGN mechanistically couples to RhoA/ROCK signaling and how its junctional scaffolding role connects to its motility-suppressing role remain unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["no direct effector linking CGN to RhoA regulation identified","structural basis of cytoskeletal and ZO-1 tethering not characterized in the corpus","mechanism controlling claudin-2 abundance unknown"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0060090","term_label":"molecular adaptor activity","supporting_discovery_ids":[0,1]},{"term_id":"GO:0008092","term_label":"cytoskeletal protein binding","supporting_discovery_ids":[1]}],"localization":[{"term_id":"GO:0005829","term_label":"cytosol","supporting_discovery_ids":[0,1]}],"pathway":[{"term_id":"R-HSA-1500931","term_label":"Cell-Cell communication","supporting_discovery_ids":[0,1]},{"term_id":"R-HSA-162582","term_label":"Signal Transduction","supporting_discovery_ids":[2]}],"complexes":["tight junction"],"partners":["ZO-1","CGNL1"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q9P2M7","full_name":"Cingulin","aliases":[],"length_aa":1203,"mass_kda":137.1,"function":"Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier","subcellular_location":"Cell junction, tight junction","url":"https://www.uniprot.org/uniprotkb/Q9P2M7/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/CGN","classification":"Not Classified","n_dependent_lines":6,"n_total_lines":1208,"dependency_fraction":0.004966887417218543},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[{"gene":"CALD1","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/search/CGN","total_profiled":1310},"omim":[{"mim_id":"619990","title":"TRANSMEMBRANE p24 TRAFFICKING PROTEIN 7; TMED7","url":"https://www.omim.org/entry/619990"},{"mim_id":"616432","title":"RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 18; ARHGEF18","url":"https://www.omim.org/entry/616432"},{"mim_id":"614398","title":"JUNCTIONAL CADHERIN 5-ASSOCIATED PROTEIN; JCAD","url":"https://www.omim.org/entry/614398"},{"mim_id":"613735","title":"BRAIN MALFORMATIONS WITH OR WITHOUT URINARY TRACT DEFECTS; BRMUTD","url":"https://www.omim.org/entry/613735"},{"mim_id":"609473","title":"CINGULIN; CGN","url":"https://www.omim.org/entry/609473"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"Supported","locations":[{"location":"Plasma membrane","reliability":"Supported"},{"location":"Cell Junctions","reliability":"Supported"}],"tissue_specificity":"Tissue enhanced","tissue_distribution":"Detected in many","driving_tissues":[{"tissue":"intestine","ntpm":53.1}],"url":"https://www.proteinatlas.org/search/CGN"},"hgnc":{"alias_symbol":["KIAA1319"],"prev_symbol":[]},"alphafold":{"accession":"Q9P2M7","domains":[{"cath_id":"1.20.5","chopping":"1113-1140","consensus_level":"medium","plddt":87.4186,"start":1113,"end":1140}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q9P2M7","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q9P2M7-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q9P2M7-F1-predicted_aligned_error_v6.png","plddt_mean":69.56},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=CGN","jax_strain_url":"https://www.jax.org/strain/search?query=CGN"},"sequence":{"accession":"Q9P2M7","fasta_url":"https://rest.uniprot.org/uniprotkb/Q9P2M7.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q9P2M7/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q9P2M7"}},"corpus_meta":[{"pmid":"34830864","id":"PMC_34830864","title":"miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.","date":"2021","source":"Cancers","url":"https://pubmed.ncbi.nlm.nih.gov/34830864","citation_count":30,"is_preprint":false},{"pmid":"23658230","id":"PMC_23658230","title":"Life without tRNAArg-adenosine deaminase TadA: evolutionary consequences of decoding the four CGN codons as arginine in Mycoplasmas and other Mollicutes.","date":"2013","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/23658230","citation_count":28,"is_preprint":false},{"pmid":"35484716","id":"PMC_35484716","title":"Single-cell RNA Sequencing Identified Novel Nr4a1+ Ear2+ Anti-Inflammatory Macrophage Phenotype under Myeloid-TLR4 Dependent Regulation in Anti-Glomerular Basement Membrane (GBM) Crescentic Glomerulonephritis (cGN).","date":"2022","source":"Advanced science (Weinheim, Baden-Wurttemberg, Germany)","url":"https://pubmed.ncbi.nlm.nih.gov/35484716","citation_count":22,"is_preprint":false},{"pmid":"25747092","id":"PMC_25747092","title":"Transformation pathways in high-pressure solid nitrogen: from molecular N2 to polymeric cg-N.","date":"2015","source":"The Journal of chemical physics","url":"https://pubmed.ncbi.nlm.nih.gov/25747092","citation_count":15,"is_preprint":false},{"pmid":"35809677","id":"PMC_35809677","title":"Biophysical interaction between lanthanum chloride and (CG)n or (GC)n repeats: A reversible B-to-Z DNA transition.","date":"2022","source":"International journal of biological macromolecules","url":"https://pubmed.ncbi.nlm.nih.gov/35809677","citation_count":11,"is_preprint":false},{"pmid":"16717448","id":"PMC_16717448","title":"Isolation, mapping and identification of SNPs for four genes (ACP6, CGN, ANXA9, SLC27A3) from a bovine QTL region on BTA3.","date":"2006","source":"Cytogenetic and genome research","url":"https://pubmed.ncbi.nlm.nih.gov/16717448","citation_count":7,"is_preprint":false},{"pmid":"37566083","id":"PMC_37566083","title":"Knock Out of CGN and CGNL1 in MDCK Cells Affects Claudin-2 but Has a Minor Impact on Tight Junction Barrier Function.","date":"2023","source":"Cells","url":"https://pubmed.ncbi.nlm.nih.gov/37566083","citation_count":6,"is_preprint":false},{"pmid":"27029281","id":"PMC_27029281","title":"Where have all the inosines gone? Conflicting evidence for A-to-I editing of the anticodon of higher eukaryotic tRNAACGArg questions the dogma of a universal wobble-mediated decoding of CGN codons.","date":"2016","source":"IUBMB life","url":"https://pubmed.ncbi.nlm.nih.gov/27029281","citation_count":5,"is_preprint":false},{"pmid":"34562582","id":"PMC_34562582","title":"Hepatic antioxidant activity, immunomodulation, and pro-anti-inflammatory cytokines manipulation of κ-carrageenan (κ-CGN) in cobia, Rachycentron canadum against Lactococcus garvieae.","date":"2021","source":"Fish & shellfish immunology","url":"https://pubmed.ncbi.nlm.nih.gov/34562582","citation_count":3,"is_preprint":false},{"pmid":"39628587","id":"PMC_39628587","title":"TLX3 regulates CGN progenitor proliferation during cerebellum development and its dysfunction can lead to autism.","date":"2024","source":"iScience","url":"https://pubmed.ncbi.nlm.nih.gov/39628587","citation_count":2,"is_preprint":false},{"pmid":"8754409","id":"PMC_8754409","title":"[Evaluation of factors influencing platelet aggregation in patients with chronic glomerulonephritis (CGN)].","date":"1996","source":"Przeglad lekarski","url":"https://pubmed.ncbi.nlm.nih.gov/8754409","citation_count":2,"is_preprint":false},{"pmid":"38778164","id":"PMC_38778164","title":"Arginines of the CGN codon family are Achilles' heels of cancer genes.","date":"2024","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/38778164","citation_count":1,"is_preprint":false},{"pmid":"8754408","id":"PMC_8754408","title":"[Evaluation of platelet membrane structure in patients with chronic glomerulonephritis (CGN)].","date":"1996","source":"Przeglad lekarski","url":"https://pubmed.ncbi.nlm.nih.gov/8754408","citation_count":1,"is_preprint":false},{"pmid":"40773225","id":"PMC_40773225","title":"Polyphyllin H inhibits malignant progression of ovarian cancer in patient-derived xenograft mouse models by regulating CGN/RhoA/Rock2 axis: an experimental research.","date":"2025","source":"International journal of surgery (London, England)","url":"https://pubmed.ncbi.nlm.nih.gov/40773225","citation_count":0,"is_preprint":false},{"pmid":null,"id":"bio_10.1101_2025.06.04.657904","title":"Tetraploid turnips (  <i>Brassica rapa</i>  ssp.  <i>rapa</i>  ) are highly tolerant of tetravalent formation and aneuploidy","date":"2025-06-06","source":"bioRxiv","url":"https://doi.org/10.1101/2025.06.04.657904","citation_count":0,"is_preprint":true}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":10141,"output_tokens":1353,"usd":0.025359,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7568,"output_tokens":1604,"usd":0.03897,"stage2_stop_reason":"end_turn"},"total_usd":0.064329,"stage1_batch_id":"msgbatch_01ACAxJQabDsht39JXeWG22d","stage2_batch_id":"msgbatch_01QEU22A9U5sbPTgsMzTmvSf","note":"batch pricing = 50% of standard"},"stage1_raw":"Looking at the corpus, I need to identify papers about the canonical protein CGN (cingulin, also known as KIAA1319 per HGNC aliases). Let me classify each paper:\n\n- Papers [1], [7], [14]: About cingulin (CGN) protein - the canonical gene. INCLUDE.\n- Papers [2], [4], [5], [8], [12]: About CGN codons (arginine codons) or nitrogen chemistry - NOT the CGN protein gene. EXCLUDE (symbol collision/unrelated).\n- Papers [3], [11], [13]: About cGN/CGN as abbreviation for \"crescentic/chronic glomerulonephritis\" - NOT the gene. EXCLUDE.\n- Paper [6]: About bovine CGN (cingulin gene) - includes identification/mapping of the cingulin gene in cattle. Minor relevance, no mechanistic findings.\n- Paper [9]: About κ-carrageenan (κ-CGN) - NOT the gene. EXCLUDE.\n- Paper [10]: About CGN progenitors (cerebellar granule neurons) - abbreviation collision. EXCLUDE.\n- Paper [15]: About CGN Wageningen (germplasm bank abbreviation). EXCLUDE.\n\nNow extracting mechanistic discoveries from papers [1], [7], [14]:\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"Knockout of CGN (cingulin) and/or CGNL1 (paracingulin) in MDCK kidney epithelial cells causes a modest but significant increase in transepithelial resistance and decreased junctional accumulation of claudin-2, with reduced claudin-2 protein levels; this phenotype was rescued by CGN or CGNL1 overexpression but not by ZO-1 overexpression, establishing that CGN regulates claudin-2 junctional localization and expression independently of ZO-1.\",\n      \"method\": \"CRISPR knockout in MDCK cells, calcium switch assay, transepithelial resistance measurement, dextran permeability assay, rescue by overexpression\",\n      \"journal\": \"Cells\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO with defined cellular phenotype and rescue experiment, single lab with multiple orthogonal methods\",\n      \"pmids\": [\"37566083\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"CGN (cingulin) plays a role in tethering ZO-1 to the actomyosin and microtubule cytoskeletons at tight junctions, and is dispensable for overall epithelial barrier function in MDCK cells.\",\n      \"method\": \"Knockout in MDCK cells, transepithelial resistance measurement, permeability assays\",\n      \"journal\": \"Cells\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — KO phenotype with functional barrier readouts, single lab\",\n      \"pmids\": [\"37566083\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"CGN (cingulin) is a direct target of miR-125b in colorectal cancer cells; miR-125b suppresses CGN expression via its 3'UTR (validated by dual-luciferase reporter assay), and CGN loss activates the RhoA/ROCK pathway to promote cell migration and invasion.\",\n      \"method\": \"Dual-luciferase reporter assay, RNA-seq, overexpression/knockdown of miR-125b and CGN in CRC cells, in vitro migration/invasion assays, in vivo xenograft\",\n      \"journal\": \"Cancers\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct 3'UTR luciferase validation plus functional in vitro and in vivo assays, single lab with multiple orthogonal methods\",\n      \"pmids\": [\"34830864\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"CGN (cingulin) upregulation by polyphyllin H blocks the RhoA/Rock2 signaling pathway in ovarian cancer cells, inhibiting proliferation, invasion, and migration; protein sequencing identified CGN as the mechanistic target mediating polyphyllin H's anti-tumor effects.\",\n      \"method\": \"CCK-8 proliferation assay, in vitro invasion/migration assays, CDX and PDX mouse models, protein sequencing/proteomics, in vivo tumor growth measurement\",\n      \"journal\": \"International journal of surgery (London, England)\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — protein sequencing-based pathway inference in a single study; mechanistic link between CGN and RhoA/Rock2 not directly validated by mutagenesis or reconstitution\",\n      \"pmids\": [\"40773225\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"Cingulin (CGN) is a cytoplasmic tight junction scaffold protein that tethers ZO-1 to the actomyosin and microtubule cytoskeletons, regulates claudin-2 junctional localization and expression independently of ZO-1, and suppresses cell migration and invasion by restraining the RhoA/ROCK signaling pathway; it is dispensable for overall epithelial paracellular barrier function.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"Cingulin (CGN) is a cytoplasmic tight junction scaffold that links the junctional adaptor ZO-1 to the actomyosin and microtubule cytoskeletons in epithelial cells [#1]. It controls the junctional accumulation and protein abundance of claudin-2, and this regulation is independent of ZO-1, as CGN or its relative CGNL1 — but not ZO-1 — rescue the loss-of-CGN phenotype [#0]. CGN is dispensable for overall epithelial paracellular barrier function, with its loss producing only a modest increase in transepithelial resistance [#0, #1]. Beyond its junctional role, CGN restrains the RhoA/ROCK signaling pathway: loss of CGN activates RhoA/ROCK to promote cell migration and invasion, and restoring CGN suppresses these behaviors in cancer cells [#2].\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"Established that CGN is a post-transcriptionally regulated suppressor of cell motility, defining its link to RhoA/ROCK signaling in cancer.\",\n      \"evidence\": \"dual-luciferase 3'UTR reporter, miR-125b/CGN overexpression and knockdown, migration/invasion assays and xenografts in colorectal cancer cells\",\n      \"pmids\": [\"34830864\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"the molecular step by which CGN restrains RhoA is not defined (no GEF/GAP or direct binding partner identified)\",\n        \"link is correlative for the signaling axis rather than reconstituted\",\n        \"generality beyond colorectal cancer untested at this point\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Defined CGN's core junctional mechanism by separating its cytoskeletal-tethering and claudin-2-regulatory functions from barrier maintenance and from ZO-1.\",\n      \"evidence\": \"CRISPR knockout of CGN/CGNL1 in MDCK cells with calcium switch, transepithelial resistance, dextran permeability, and overexpression rescue\",\n      \"pmids\": [\"37566083\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"mechanism by which CGN controls claudin-2 protein levels (transcriptional vs stability) unresolved\",\n        \"single cell line (MDCK) and single lab\",\n        \"direct binding interfaces to ZO-1, actomyosin, and microtubules not mapped here\"\n      ]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Extended the CGN–RhoA/ROCK suppressor axis to ovarian cancer and nominated CGN as a druggable target of a natural compound.\",\n      \"evidence\": \"polyphyllin H treatment with proteomics/protein sequencing, proliferation/invasion/migration assays, CDX and PDX models\",\n      \"pmids\": [\"40773225\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"CGN-RhoA/Rock2 link inferred from protein sequencing, not validated by mutagenesis or reconstitution\",\n        \"whether polyphyllin H acts directly or indirectly on CGN is unknown\",\n        \"not independently confirmed\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How CGN mechanistically couples to RhoA/ROCK signaling and how its junctional scaffolding role connects to its motility-suppressing role remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"no direct effector linking CGN to RhoA regulation identified\",\n        \"structural basis of cytoskeletal and ZO-1 tethering not characterized in the corpus\",\n        \"mechanism controlling claudin-2 abundance unknown\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0060090\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"GO:0008092\", \"supporting_discovery_ids\": [1]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005829\", \"supporting_discovery_ids\": [0, 1]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-1500931\", \"supporting_discovery_ids\": [0, 1]},\n      {\"term_id\": \"R-HSA-162582\", \"supporting_discovery_ids\": [2]}\n    ],\n    \"complexes\": [\"tight junction\"],\n    \"partners\": [\"ZO-1\", \"CGNL1\"],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":4,"faith_total":4,"faith_pct":100.0}}