{"gene":"CCER1","run_date":"2026-06-09T22:57:17","timeline":{"discoveries":[{"year":2007,"finding":"C12orf12 (CCER1) promoter CpG island is densely methylated in normal somatic tissues, leading to gene silencing, while remaining unmethylated (and expressed) in testis and sperm; silencing is dependent on DNMT1 and/or DNMT3b, as reactivation occurs in cell lines lacking these methyltransferases.","method":"Methylated CpG island amplification with microarrays, bisulfite cloning and sequencing, treatment with DNA demethylating agent, analysis of DNMT1/DNMT3b-deficient cell lines","journal":"PLoS genetics","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — multiple orthogonal methods (array profiling, bisulfite sequencing, demethylating agent treatment, DNMT-KO cell lines) in a single study establishing the methylation-silencing mechanism for this gene","pmids":["17967063"],"is_preprint":false},{"year":2023,"finding":"CCER1 is a germline-specific intrinsically disordered protein (IDP) that self-assembles into liquid-liquid phase-separated nuclear condensates in spermatids; these condensates are immiscible with heterochromatin and are required for the histone-to-protamine (HTP) transition by promoting transcription of transition proteins (Tnp1/2) and protamines (Prm1/2) and mediating multiple histone epigenetic modifications. CCER1 deficiency causes defective sperm chromatin compaction and infertility in mice.","method":"Ccer1 knockout mice (loss-of-function phenotype), live-cell imaging of phase-separated condensates, gene expression analysis of Tnp1/2 and Prm1/2, histone modification assays, identification of human LoF variants in NOA patients, in vitro condensate disruption assays with patient-derived mutants","journal":"Nature communications","confidence":"High","confidence_rationale":"Tier 2 / Strong — KO mouse model with defined cellular phenotype, phase-separation imaging, histone modification profiling, and human variant functional validation across multiple orthogonal methods in a single study; replicated by independent lab (PMID:39868420)","pmids":["38081819"],"is_preprint":false},{"year":2024,"finding":"Ccer1 knockout mice exhibit subfertility with altered sperm head and tail ultrastructure, confirming CCER1 as a spermatid-specific gene critical for spermiogenesis; CCER1 expression is enriched during spermiogenesis in spermatids in both mice and humans.","method":"Ccer1 knockout mouse generation, sperm ultrastructure analysis (electron microscopy implied), expression profiling in mouse and human spermatids","journal":"The International journal of developmental biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — independent KO mouse study with direct ultrastructural phenotyping, replicating and extending findings of PMID:38081819 in a different laboratory","pmids":["39868420"],"is_preprint":false},{"year":2026,"finding":"CCER1 nuclear condensates recruit the TIP60/EPC1/NuA4 acetyltransferase complex; CCER1 acts as a 'reaction compartment' for mutual recruitment of TIP60 and EPC1 subunits. Disruption of CCER1 droplets impairs TIP60 and EPC1 recruitment, reduces histone H4 hyperacetylation in nucleosomes, causes defective DNA strand breakage, and leads to insufficient histone-to-protamine (HTP) replacement during spermiogenesis. A humanized CCER1 knock-in mouse line demonstrates conservation of function between mouse and human CCER1.","method":"Immunoprecipitation-mass spectrometry (IP-MS) with Ccer1-Tag knock-in mice, humanized CCER1 knock-in mouse line, histone H4 acetylation assays, DNA strand break assays, condensate disruption experiments","journal":"Development (Cambridge, England)","confidence":"High","confidence_rationale":"Tier 1–2 / Moderate — IP-MS interactome identification combined with functional knockin models, histone modification assays, and DNA break assays providing multiple orthogonal lines of evidence in a single focused mechanistic study","pmids":["41670399"],"is_preprint":false}],"current_model":"CCER1 is a germline-specific, intrinsically disordered protein that forms liquid-liquid phase-separated nuclear condensates in postmeiotic spermatids; these condensates recruit the TIP60/EPC1/NuA4 acetyltransferase complex, promote histone H4 hyperacetylation and DNA strand breakage, drive transcription of transition proteins and protamines, and thereby coordinate the histone-to-protamine transition required for sperm chromatin compaction and male fertility, while in somatic tissues the gene is silenced by dense promoter CpG island methylation dependent on DNMT1/DNMT3b."},"narrative":{"mechanistic_narrative":"CCER1 is a germline-specific, intrinsically disordered protein that organizes the histone-to-protamine transition required for sperm chromatin compaction during spermiogenesis [PMID:38081819, PMID:39868420]. In postmeiotic spermatids, CCER1 self-assembles into liquid-liquid phase-separated nuclear condensates that are immiscible with heterochromatin and drive transcription of transition proteins (Tnp1/2) and protamines (Prm1/2) [PMID:38081819]. These condensates act as reaction compartments that recruit the TIP60/EPC1/NuA4 acetyltransferase complex through mutual recruitment of its TIP60 and EPC1 subunits, promoting nucleosomal histone H4 hyperacetylation and DNA strand breakage that license histone replacement [PMID:41670399]. Loss of CCER1 or disruption of its condensates impairs TIP60/EPC1 recruitment, reduces H4 hyperacetylation, blocks DNA strand breakage, and produces defective sperm chromatin compaction; human loss-of-function variants are found in non-obstructive azoospermia patients and CCER1-deficient mice are infertile or subfertile with abnormal sperm head and tail ultrastructure [PMID:38081819, PMID:39868420, PMID:41670399]. In somatic tissues the gene is held silent by dense promoter CpG island methylation dependent on DNMT1 and DNMT3b, while remaining unmethylated and expressed in testis and sperm [PMID:17967063].","teleology":[{"year":2007,"claim":"Established that CCER1 (C12orf12) is restricted to the germline through epigenetic control, defining it as a cancer/testis-type gene silenced in soma by promoter methylation.","evidence":"Methylated CpG island array profiling, bisulfite sequencing, demethylating-agent treatment, and analysis of DNMT1/DNMT3b-deficient cell lines","pmids":["17967063"],"confidence":"Medium","gaps":["Did not address the protein's molecular function in testis","Mechanism by which the testis escapes methylation not defined","No germline phenotype examined"]},{"year":2023,"claim":"Defined CCER1's core function: an intrinsically disordered protein that forms phase-separated nuclear condensates in spermatids driving the histone-to-protamine transition and required for fertility.","evidence":"Ccer1 knockout mice, live-cell condensate imaging, Tnp1/2 and Prm1/2 expression analysis, histone modification assays, and functional validation of human NOA variants","pmids":["38081819"],"confidence":"High","gaps":["Did not identify the protein partners recruited to condensates","Mechanistic link between condensates and specific histone modifications not resolved","Determinants of condensate immiscibility with heterochromatin unknown"]},{"year":2024,"claim":"Independently confirmed CCER1 as a spermatid-specific spermiogenesis factor by demonstrating sperm head and tail ultrastructural defects in knockout mice.","evidence":"Independent Ccer1 knockout mouse with sperm ultrastructure analysis and spermatid expression profiling in mouse and human","pmids":["39868420"],"confidence":"Medium","gaps":["Subfertility severity differs from the earlier infertility report without reconciliation","Did not add molecular mechanism beyond phenotype confirmation"]},{"year":2026,"claim":"Resolved the molecular activity of CCER1 condensates by showing they function as reaction compartments that recruit the TIP60/EPC1/NuA4 acetyltransferase complex to drive H4 hyperacetylation and DNA strand breakage during histone replacement.","evidence":"IP-MS with Ccer1-Tag knock-in mice, humanized CCER1 knock-in line, H4 acetylation assays, DNA strand break assays, and condensate disruption experiments","pmids":["41670399"],"confidence":"High","gaps":["How CCER1 selectively recruits TIP60 versus other NuA4 subunits not defined","Connection between H4 hyperacetylation, strand breakage, and protamine deposition not mechanistically dissected","Structural basis of condensate-mediated complex assembly unknown"]},{"year":null,"claim":"How CCER1 condensates couple transcriptional activation of protamine/transition-protein genes to the chromatin-remodeling machinery, and the full condensate interactome, remain to be defined.","evidence":"No single experiment yet integrates the transcriptional and chromatin-remodeling arms of CCER1 function","pmids":[],"confidence":"Low","gaps":["No structural model of CCER1 condensate assembly","Full interactome beyond TIP60/EPC1 not catalogued","Regulation of CCER1 condensate dynamics during spermatid maturation unknown"]}],"mechanism_profile":{"molecular_activity":[{"term_id":"GO:0140110","term_label":"transcription regulator activity","supporting_discovery_ids":[1]},{"term_id":"GO:0060090","term_label":"molecular adaptor activity","supporting_discovery_ids":[3]},{"term_id":"GO:0042393","term_label":"histone binding","supporting_discovery_ids":[3]}],"localization":[{"term_id":"GO:0005634","term_label":"nucleus","supporting_discovery_ids":[1,3]}],"pathway":[{"term_id":"R-HSA-1474165","term_label":"Reproduction","supporting_discovery_ids":[1,2]},{"term_id":"R-HSA-4839726","term_label":"Chromatin organization","supporting_discovery_ids":[3]}],"complexes":[],"partners":["TIP60","EPC1"],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q8TC90","full_name":"Coiled-coil domain-containing glutamate-rich protein 1","aliases":[],"length_aa":406,"mass_kda":46.5,"function":"Regulator of histone epigenetic modifications and chromatin compaction into the sperm head, required for histone-to-protamine (HTP) transition. HTP is a key event in which somatic histones are first replaced by testis-specific histone variants, then transition proteins (TNPs) are incorporated into the spermatid nucleus, and finally protamines (PRMs) replace the TNPs to promote chromatin condensation","subcellular_location":"Nucleus","url":"https://www.uniprot.org/uniprotkb/Q8TC90/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/CCER1","classification":"Not Classified","n_dependent_lines":1,"n_total_lines":1208,"dependency_fraction":0.0008278145695364238},"opencell":{"profiled":false,"resolved_as":"","ensg_id":"","cell_line_id":"","localizations":[],"interactors":[],"url":"https://opencell.sf.czbiohub.org/search/CCER1","total_profiled":1310},"omim":[{"mim_id":"620881","title":"COILED-COIL GLUTAMATE-RICH PROTEIN 1; CCER1","url":"https://www.omim.org/entry/620881"},{"mim_id":"612868","title":"CORNEAL DYSTROPHY, POSTERIOR AMORPHOUS; PACD","url":"https://www.omim.org/entry/612868"}],"hpa":{"profiled":true,"resolved_as":"","reliability":"","locations":[],"tissue_specificity":"Tissue enriched","tissue_distribution":"Detected in single","driving_tissues":[{"tissue":"testis","ntpm":36.2}],"url":"https://www.proteinatlas.org/search/CCER1"},"hgnc":{"alias_symbol":["MGC26598"],"prev_symbol":["C12orf12"]},"alphafold":{"accession":"Q8TC90","domains":[],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q8TC90","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q8TC90-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q8TC90-F1-predicted_aligned_error_v6.png","plddt_mean":50.12},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=CCER1","jax_strain_url":"https://www.jax.org/strain/search?query=CCER1"},"sequence":{"accession":"Q8TC90","fasta_url":"https://rest.uniprot.org/uniprotkb/Q8TC90.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q8TC90/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q8TC90"}},"corpus_meta":[{"pmid":"17967063","id":"PMC_17967063","title":"Genome-wide profiling of DNA methylation reveals a class of normally methylated CpG island promoters.","date":"2007","source":"PLoS genetics","url":"https://pubmed.ncbi.nlm.nih.gov/17967063","citation_count":275,"is_preprint":false},{"pmid":"38081819","id":"PMC_38081819","title":"Phase-separated CCER1 coordinates the histone-to-protamine transition and male fertility.","date":"2023","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/38081819","citation_count":20,"is_preprint":false},{"pmid":"39868420","id":"PMC_39868420","title":"Ccer1 is a spermatid-specific gene required for spermatogenesis and male fertility.","date":"2024","source":"The International journal of developmental biology","url":"https://pubmed.ncbi.nlm.nih.gov/39868420","citation_count":2,"is_preprint":false},{"pmid":"41670399","id":"PMC_41670399","title":"CCER1 condensates participate in histone-to-protamine transition by recruiting the TIP60/EPC1/NuA4 acetyltransferase complex.","date":"2026","source":"Development (Cambridge, England)","url":"https://pubmed.ncbi.nlm.nih.gov/41670399","citation_count":0,"is_preprint":false},{"pmid":"41269209","id":"PMC_41269209","title":"HPV Integration in Head and Neck Cancer: Downstream Splicing Events and Expression Ratios Linked with Poor Outcomes.","date":"2026","source":"Clinical cancer research : an official journal of the American Association for Cancer Research","url":"https://pubmed.ncbi.nlm.nih.gov/41269209","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4616,"output_tokens":1372,"usd":0.017214,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":8015,"output_tokens":2116,"usd":0.046488,"stage2_stop_reason":"end_turn"},"total_usd":0.063702,"stage1_batch_id":"msgbatch_01ACAxJQabDsht39JXeWG22d","stage2_batch_id":"msgbatch_01QEU22A9U5sbPTgsMzTmvSf","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2007,\n      \"finding\": \"C12orf12 (CCER1) promoter CpG island is densely methylated in normal somatic tissues, leading to gene silencing, while remaining unmethylated (and expressed) in testis and sperm; silencing is dependent on DNMT1 and/or DNMT3b, as reactivation occurs in cell lines lacking these methyltransferases.\",\n      \"method\": \"Methylated CpG island amplification with microarrays, bisulfite cloning and sequencing, treatment with DNA demethylating agent, analysis of DNMT1/DNMT3b-deficient cell lines\",\n      \"journal\": \"PLoS genetics\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — multiple orthogonal methods (array profiling, bisulfite sequencing, demethylating agent treatment, DNMT-KO cell lines) in a single study establishing the methylation-silencing mechanism for this gene\",\n      \"pmids\": [\"17967063\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"CCER1 is a germline-specific intrinsically disordered protein (IDP) that self-assembles into liquid-liquid phase-separated nuclear condensates in spermatids; these condensates are immiscible with heterochromatin and are required for the histone-to-protamine (HTP) transition by promoting transcription of transition proteins (Tnp1/2) and protamines (Prm1/2) and mediating multiple histone epigenetic modifications. CCER1 deficiency causes defective sperm chromatin compaction and infertility in mice.\",\n      \"method\": \"Ccer1 knockout mice (loss-of-function phenotype), live-cell imaging of phase-separated condensates, gene expression analysis of Tnp1/2 and Prm1/2, histone modification assays, identification of human LoF variants in NOA patients, in vitro condensate disruption assays with patient-derived mutants\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — KO mouse model with defined cellular phenotype, phase-separation imaging, histone modification profiling, and human variant functional validation across multiple orthogonal methods in a single study; replicated by independent lab (PMID:39868420)\",\n      \"pmids\": [\"38081819\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"Ccer1 knockout mice exhibit subfertility with altered sperm head and tail ultrastructure, confirming CCER1 as a spermatid-specific gene critical for spermiogenesis; CCER1 expression is enriched during spermiogenesis in spermatids in both mice and humans.\",\n      \"method\": \"Ccer1 knockout mouse generation, sperm ultrastructure analysis (electron microscopy implied), expression profiling in mouse and human spermatids\",\n      \"journal\": \"The International journal of developmental biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — independent KO mouse study with direct ultrastructural phenotyping, replicating and extending findings of PMID:38081819 in a different laboratory\",\n      \"pmids\": [\"39868420\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2026,\n      \"finding\": \"CCER1 nuclear condensates recruit the TIP60/EPC1/NuA4 acetyltransferase complex; CCER1 acts as a 'reaction compartment' for mutual recruitment of TIP60 and EPC1 subunits. Disruption of CCER1 droplets impairs TIP60 and EPC1 recruitment, reduces histone H4 hyperacetylation in nucleosomes, causes defective DNA strand breakage, and leads to insufficient histone-to-protamine (HTP) replacement during spermiogenesis. A humanized CCER1 knock-in mouse line demonstrates conservation of function between mouse and human CCER1.\",\n      \"method\": \"Immunoprecipitation-mass spectrometry (IP-MS) with Ccer1-Tag knock-in mice, humanized CCER1 knock-in mouse line, histone H4 acetylation assays, DNA strand break assays, condensate disruption experiments\",\n      \"journal\": \"Development (Cambridge, England)\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Moderate — IP-MS interactome identification combined with functional knockin models, histone modification assays, and DNA break assays providing multiple orthogonal lines of evidence in a single focused mechanistic study\",\n      \"pmids\": [\"41670399\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"CCER1 is a germline-specific, intrinsically disordered protein that forms liquid-liquid phase-separated nuclear condensates in postmeiotic spermatids; these condensates recruit the TIP60/EPC1/NuA4 acetyltransferase complex, promote histone H4 hyperacetylation and DNA strand breakage, drive transcription of transition proteins and protamines, and thereby coordinate the histone-to-protamine transition required for sperm chromatin compaction and male fertility, while in somatic tissues the gene is silenced by dense promoter CpG island methylation dependent on DNMT1/DNMT3b.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"CCER1 is a germline-specific, intrinsically disordered protein that organizes the histone-to-protamine transition required for sperm chromatin compaction during spermiogenesis [#1, #2]. In postmeiotic spermatids, CCER1 self-assembles into liquid-liquid phase-separated nuclear condensates that are immiscible with heterochromatin and drive transcription of transition proteins (Tnp1/2) and protamines (Prm1/2) [#1]. These condensates act as reaction compartments that recruit the TIP60/EPC1/NuA4 acetyltransferase complex through mutual recruitment of its TIP60 and EPC1 subunits, promoting nucleosomal histone H4 hyperacetylation and DNA strand breakage that license histone replacement [#3]. Loss of CCER1 or disruption of its condensates impairs TIP60/EPC1 recruitment, reduces H4 hyperacetylation, blocks DNA strand breakage, and produces defective sperm chromatin compaction; human loss-of-function variants are found in non-obstructive azoospermia patients and CCER1-deficient mice are infertile or subfertile with abnormal sperm head and tail ultrastructure [#1, #2, #3]. In somatic tissues the gene is held silent by dense promoter CpG island methylation dependent on DNMT1 and DNMT3b, while remaining unmethylated and expressed in testis and sperm [#0].\",\n  \"teleology\": [\n    {\n      \"year\": 2007,\n      \"claim\": \"Established that CCER1 (C12orf12) is restricted to the germline through epigenetic control, defining it as a cancer/testis-type gene silenced in soma by promoter methylation.\",\n      \"evidence\": \"Methylated CpG island array profiling, bisulfite sequencing, demethylating-agent treatment, and analysis of DNMT1/DNMT3b-deficient cell lines\",\n      \"pmids\": [\"17967063\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Did not address the protein's molecular function in testis\",\n        \"Mechanism by which the testis escapes methylation not defined\",\n        \"No germline phenotype examined\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Defined CCER1's core function: an intrinsically disordered protein that forms phase-separated nuclear condensates in spermatids driving the histone-to-protamine transition and required for fertility.\",\n      \"evidence\": \"Ccer1 knockout mice, live-cell condensate imaging, Tnp1/2 and Prm1/2 expression analysis, histone modification assays, and functional validation of human NOA variants\",\n      \"pmids\": [\"38081819\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Did not identify the protein partners recruited to condensates\",\n        \"Mechanistic link between condensates and specific histone modifications not resolved\",\n        \"Determinants of condensate immiscibility with heterochromatin unknown\"\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Independently confirmed CCER1 as a spermatid-specific spermiogenesis factor by demonstrating sperm head and tail ultrastructural defects in knockout mice.\",\n      \"evidence\": \"Independent Ccer1 knockout mouse with sperm ultrastructure analysis and spermatid expression profiling in mouse and human\",\n      \"pmids\": [\"39868420\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Subfertility severity differs from the earlier infertility report without reconciliation\",\n        \"Did not add molecular mechanism beyond phenotype confirmation\"\n      ]\n    },\n    {\n      \"year\": 2026,\n      \"claim\": \"Resolved the molecular activity of CCER1 condensates by showing they function as reaction compartments that recruit the TIP60/EPC1/NuA4 acetyltransferase complex to drive H4 hyperacetylation and DNA strand breakage during histone replacement.\",\n      \"evidence\": \"IP-MS with Ccer1-Tag knock-in mice, humanized CCER1 knock-in line, H4 acetylation assays, DNA strand break assays, and condensate disruption experiments\",\n      \"pmids\": [\"41670399\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"How CCER1 selectively recruits TIP60 versus other NuA4 subunits not defined\",\n        \"Connection between H4 hyperacetylation, strand breakage, and protamine deposition not mechanistically dissected\",\n        \"Structural basis of condensate-mediated complex assembly unknown\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How CCER1 condensates couple transcriptional activation of protamine/transition-protein genes to the chromatin-remodeling machinery, and the full condensate interactome, remain to be defined.\",\n      \"evidence\": \"No single experiment yet integrates the transcriptional and chromatin-remodeling arms of CCER1 function\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No structural model of CCER1 condensate assembly\",\n        \"Full interactome beyond TIP60/EPC1 not catalogued\",\n        \"Regulation of CCER1 condensate dynamics during spermatid maturation unknown\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0140110\", \"supporting_discovery_ids\": [1]},\n      {\"term_id\": \"GO:0060090\", \"supporting_discovery_ids\": [3]},\n      {\"term_id\": \"GO:0042393\", \"supporting_discovery_ids\": [3]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [1, 3]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"R-HSA-1474165\", \"supporting_discovery_ids\": [1, 2]},\n      {\"term_id\": \"R-HSA-4839726\", \"supporting_discovery_ids\": [3]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"TIP60\", \"EPC1\"],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"pairwise":"win","faith_supported":5,"faith_total":5,"faith_pct":100.0}}