{"gene":"ANKRD46","run_date":"2026-06-09T22:02:43","timeline":{"discoveries":[{"year":2011,"finding":"ANKRD46 is a direct transcriptional target of miR-21; miR-21 binds the 3' UTR of ANKRD46 and suppresses its expression, as demonstrated by luciferase reporter assay and Western blot showing increased ANKRD46 protein upon miR-21 knockdown in breast cancer cells.","method":"Luciferase reporter assay with 3' UTR construct, Western blot, LNA-based miR-21 knockdown with microarray","journal":"Breast cancer research : BCR","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — luciferase reporter assay with 3' UTR validation and Western blot, single lab, two orthogonal methods","pmids":["21219636"],"is_preprint":false},{"year":2014,"finding":"Ankrd46 is a direct target of miR-451 in mouse luminal epithelium; miR-451 binds and represses Ankrd46, and this interaction is implicated in embryo implantation, as shown by dual-luciferase assay and loss-of-function/gain-of-function experiments in vivo.","method":"Dual-luciferase reporter assay, miR-451 inhibitor/sponge loss-of-function in vivo, qRT-PCR, microarray","journal":"Fertility and sterility","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — luciferase reporter validation plus in vivo loss-of-function, single lab, multiple methods","pmids":["25542822"],"is_preprint":false},{"year":2014,"finding":"ANKRD46 is a validated miR-21 target in hepatocellular carcinoma cells; uptake of anti-miR-21 induces ANKRD46 expression and inhibits cell growth, confirming miR-21-mediated repression of ANKRD46 in this context.","method":"Anti-miR-21 treatment with target gene expression readout (ANKRD46 induction), shRNA screen for uptake determinants","journal":"Nucleic acids research","confidence":"Low","confidence_rationale":"Tier 3 / Weak — ANKRD46 induction used as a functional readout but no direct mechanistic experiments on ANKRD46 itself; single lab, single method for this gene","pmids":["25550434"],"is_preprint":false},{"year":2023,"finding":"ANKRD46 expression is associated with the IL6-JAK-STAT3 signaling pathway in keloid and T2DM fibrotic contexts, and miR-21 regulates ANKRD46 expression within the fibrotic microenvironment; validated by Western blotting, qRT-PCR, IHC, and flow cytometry in mouse models.","method":"Western blotting, qRT-PCR, IHC, flow cytometry, mouse models, LASSO/SVM-RFE machine learning for biomarker identification","journal":"International journal of surgery (London, England)","confidence":"Low","confidence_rationale":"Tier 3 / Weak — functional association with IL6-JAK-STAT3 pathway reported with multiple experimental validation methods but mechanistic details are limited in the abstract; single lab","pmids":["41346282"],"is_preprint":false},{"year":2023,"finding":"Circ_PRDM5 acts as a sponge for miR-25-3p, which in turn targets ANKRD46; circ_PRDM5 overexpression induces ANKRD46 upregulation via miR-25-3p sponging, and inhibition of miR-25-3p increases ANKRD46 levels and retards breast cancer progression, establishing ANKRD46 as a downstream effector of the circ_PRDM5/miR-25-3p axis.","method":"Dual-luciferase reporter assay, Western blot, MTT assay, EdU proliferation assay, transwell migration/invasion, xenograft tumor assay, qRT-PCR","journal":"Journal of biochemical and molecular toxicology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — luciferase reporter and functional cell assays confirm ANKRD46 as miR-25-3p target, but mechanistic role of ANKRD46 protein itself is not directly characterized; single lab","pmids":["37485755"],"is_preprint":false}],"current_model":"ANKRD46 is a direct target of multiple miRNAs (miR-21, miR-451, miR-25-3p), whose 3' UTR is bound and repressed by these miRNAs; it functions as a downstream effector in breast cancer cell growth suppression, embryo implantation, and fibrotic signaling (potentially via the IL6-JAK-STAT3 pathway), but its intrinsic molecular function and biochemical activity remain undefined."},"narrative":{"mechanistic_narrative":"ANKRD46 is consistently identified as a 3' UTR-targeted effector gene regulated by oncogenic and tissue-context miRNAs, rather than through any defined intrinsic biochemical activity [PMID:21219636, PMID:25542822]. It is a direct target of miR-21, which binds its 3' UTR and represses its expression in breast cancer cells, such that miR-21 knockdown increases ANKRD46 protein [PMID:21219636]. In mouse luminal epithelium, Ankrd46 is repressed by miR-451 in a manner implicated in embryo implantation [PMID:25542822]. The intrinsic molecular function and biochemical activity of the ANKRD46 protein have not been characterized in the available corpus; all available evidence positions it as a downstream node of miRNA-mediated regulation across cancer and reproductive tissues.","teleology":[{"year":2011,"claim":"Established ANKRD46 as a bona fide direct target of an oncogenic miRNA, defining the first regulatory input into this otherwise uncharacterized gene.","evidence":"Luciferase 3' UTR reporter, Western blot, and LNA-based miR-21 knockdown in breast cancer cells","pmids":["21219636"],"confidence":"Medium","gaps":["No characterization of ANKRD46 protein function or biochemical activity","Whether ANKRD46 mediates downstream phenotypes of miR-21 was not tested","No subcellular localization or interaction data"]},{"year":2014,"claim":"Extended ANKRD46 regulation beyond cancer to a developmental context by showing miR-451 represses it in luminal epithelium during embryo implantation.","evidence":"Dual-luciferase reporter assay with in vivo miR-451 loss-of-function (inhibitor/sponge) and qRT-PCR in mouse","pmids":["25542822"],"confidence":"Medium","gaps":["Mechanistic role of Ankrd46 protein in implantation not defined","Downstream effectors of Ankrd46 unknown","No demonstration of direct phenotypic rescue by Ankrd46 modulation"]},{"year":2014,"claim":"Reinforced miR-21-mediated repression of ANKRD46 in a second cancer type, using ANKRD46 induction as a readout of anti-miR-21 activity.","evidence":"Anti-miR-21 treatment with ANKRD46 expression readout in hepatocellular carcinoma cells","pmids":["25550434"],"confidence":"Low","gaps":["ANKRD46 used only as a functional readout, no direct mechanistic experiments on the gene","Causal contribution of ANKRD46 to growth inhibition not isolated"]},{"year":2023,"claim":"Placed ANKRD46 within the circ_PRDM5/miR-25-3p competing-endogenous-RNA axis in breast cancer, adding a third miRNA regulator.","evidence":"Dual-luciferase reporter, Western blot, proliferation/migration assays, and xenograft in breast cancer","pmids":["37485755"],"confidence":"Low","gaps":["Mechanistic role of ANKRD46 protein itself not characterized","How ANKRD46 retards tumor progression at the molecular level is unknown","Direct binding of miR-25-3p to ANKRD46 versus indirect effects not fully separated"]},{"year":2023,"claim":"Associated ANKRD46 expression with the IL6-JAK-STAT3 pathway in fibrotic tissue, hinting at a pathway context but without defining ANKRD46's molecular action.","evidence":"Western blot, qRT-PCR, IHC, flow cytometry, and machine-learning biomarker analysis in keloid/T2DM mouse models","pmids":["41346282"],"confidence":"Low","gaps":["Association reported without mechanistic linkage of ANKRD46 to IL6-JAK-STAT3 signaling","Whether ANKRD46 acts upstream or downstream of the pathway is undetermined","No direct biochemical or interaction data"]},{"year":null,"claim":"The intrinsic molecular function, biochemical activity, subcellular localization, and physical interaction partners of the ANKRD46 protein remain undefined.","evidence":"No discovery in the corpus directly characterizes the ANKRD46 protein","pmids":[],"confidence":"Low","gaps":["No enzymatic or binding activity assigned","No localization or complex membership established","Downstream effectors mediating its phenotypic roles unknown"]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":[]}},"prefetch_data":{"uniprot":{"accession":"Q86W74","full_name":"Ankyrin repeat domain-containing protein 46","aliases":["Ankyrin repeat small protein","ANK-S"],"length_aa":228,"mass_kda":25.3,"function":"","subcellular_location":"Membrane","url":"https://www.uniprot.org/uniprotkb/Q86W74/entry"},"depmap":{"release":"DepMap","has_data":true,"is_common_essential":false,"resolved_as":"","url":"https://depmap.org/portal/gene/ANKRD46","classification":"Not Classified","n_dependent_lines":0,"n_total_lines":1208,"dependency_fraction":0.0},"opencell":{"profiled":true,"resolved_as":"","ensg_id":"ENSG00000186106","cell_line_id":"CID001394","localizations":[{"compartment":"er","grade":3},{"compartment":"vesicles","grade":1}],"interactors":[{"gene":"MGRN1","stoichiometry":4.0},{"gene":"SPCS3","stoichiometry":0.2},{"gene":"BNIP1","stoichiometry":0.2},{"gene":"FAF2","stoichiometry":0.2},{"gene":"LMAN2L","stoichiometry":0.2},{"gene":"TMX1","stoichiometry":0.2},{"gene":"SEC62","stoichiometry":0.2},{"gene":"SRPRB","stoichiometry":0.2},{"gene":"VEZT","stoichiometry":0.2},{"gene":"NBAS","stoichiometry":0.2}],"url":"https://opencell.sf.czbiohub.org/target/CID001394","total_profiled":1310},"omim":[],"hpa":{"profiled":true,"resolved_as":"","reliability":"Approved","locations":[{"location":"Cytosol","reliability":"Approved"}],"tissue_specificity":"Low tissue specificity","tissue_distribution":"Detected in all","driving_tissues":[],"url":"https://www.proteinatlas.org/search/ANKRD46"},"hgnc":{"alias_symbol":[],"prev_symbol":[]},"alphafold":{"accession":"Q86W74","domains":[{"cath_id":"1.25.40.20","chopping":"14-126","consensus_level":"high","plddt":90.7922,"start":14,"end":126}],"viewer_url":"https://alphafold.ebi.ac.uk/entry/Q86W74","model_url":"https://alphafold.ebi.ac.uk/files/AF-Q86W74-F1-model_v6.cif","pae_url":"https://alphafold.ebi.ac.uk/files/AF-Q86W74-F1-predicted_aligned_error_v6.png","plddt_mean":77.12},"mouse_models":{"mgi_url":"https://www.informatics.jax.org/marker/summary?nomen=ANKRD46","jax_strain_url":"https://www.jax.org/strain/search?query=ANKRD46"},"sequence":{"accession":"Q86W74","fasta_url":"https://rest.uniprot.org/uniprotkb/Q86W74.fasta","uniprot_url":"https://www.uniprot.org/uniprotkb/Q86W74/entry","alphafold_viewer_url":"https://alphafold.ebi.ac.uk/entry/Q86W74"}},"corpus_meta":[{"pmid":"21219636","id":"PMC_21219636","title":"Knockdown of miR-21 in human breast cancer cell lines inhibits proliferation, in vitro migration and in vivo tumor growth.","date":"2011","source":"Breast cancer research : BCR","url":"https://pubmed.ncbi.nlm.nih.gov/21219636","citation_count":266,"is_preprint":false},{"pmid":"22559327","id":"PMC_22559327","title":"Identification of genes with a correlation between copy number and expression in gastric cancer.","date":"2012","source":"BMC medical genomics","url":"https://pubmed.ncbi.nlm.nih.gov/22559327","citation_count":94,"is_preprint":false},{"pmid":"25550434","id":"PMC_25550434","title":"Identification of the endosomal sorting complex required for transport-I (ESCRT-I) as an important modulator of anti-miR uptake by cancer cells.","date":"2014","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/25550434","citation_count":47,"is_preprint":false},{"pmid":"25542822","id":"PMC_25542822","title":"MicroRNA-451 plays a role in murine embryo implantation through targeting Ankrd46, as implicated by a microarray-based analysis.","date":"2014","source":"Fertility and sterility","url":"https://pubmed.ncbi.nlm.nih.gov/25542822","citation_count":28,"is_preprint":false},{"pmid":"33665188","id":"PMC_33665188","title":"Genome-Wide Analysis of Differentially Expressed miRNAs and Their Associated Regulatory Networks in Lenses Deficient for the Congenital Cataract-Linked Tudor Domain Containing Protein TDRD7.","date":"2021","source":"Frontiers in cell and developmental biology","url":"https://pubmed.ncbi.nlm.nih.gov/33665188","citation_count":18,"is_preprint":false},{"pmid":"37915799","id":"PMC_37915799","title":"A whole exome sequencing study to identify rare variants in multiplex families with alcohol use disorder.","date":"2023","source":"Frontiers in psychiatry","url":"https://pubmed.ncbi.nlm.nih.gov/37915799","citation_count":9,"is_preprint":false},{"pmid":"37485755","id":"PMC_37485755","title":"Circ_PRDM5/miR-25-3p/ANKRD46 axis is associated with cell malignant behaviors in subjects with breast cancer evaluated by ultrasound.","date":"2023","source":"Journal of biochemical and molecular toxicology","url":"https://pubmed.ncbi.nlm.nih.gov/37485755","citation_count":7,"is_preprint":false},{"pmid":"26722421","id":"PMC_26722421","title":"Amplification of chromosome 8q21-qter associated with the acquired paclitaxel resistance of nasopharyngeal carcinoma cells.","date":"2015","source":"International journal of clinical and experimental pathology","url":"https://pubmed.ncbi.nlm.nih.gov/26722421","citation_count":7,"is_preprint":false},{"pmid":"36684175","id":"PMC_36684175","title":"Postmenopausal osteoporosis: Effect of moderate-intensity treadmill exercise on bone proteomics in ovariectomized rats.","date":"2023","source":"Frontiers in surgery","url":"https://pubmed.ncbi.nlm.nih.gov/36684175","citation_count":6,"is_preprint":false},{"pmid":"37189611","id":"PMC_37189611","title":"Exploration and Clinical Verification of the Blood Co-Expression Genes of Type 2 Diabetes Mellitus and Mild Cognitive Dysfunction in the Elderly.","date":"2023","source":"Biomedicines","url":"https://pubmed.ncbi.nlm.nih.gov/37189611","citation_count":4,"is_preprint":false},{"pmid":"41346282","id":"PMC_41346282","title":"ANKRD46 as a shared diagnostic and therapeutic marker in keloid and type 2 diabetes mellitus identified via multi omics and experimental validation.","date":"2025","source":"International journal of surgery (London, England)","url":"https://pubmed.ncbi.nlm.nih.gov/41346282","citation_count":1,"is_preprint":false},{"pmid":null,"id":"bio_10.1101_2024.11.25.24317869","title":"Genomic predictors of response to antidepressants in Major depressive disorder (MDD): A GWAS-Based Study on Indian cohort","date":"2024-11-26","source":"bioRxiv","url":"https://doi.org/10.1101/2024.11.25.24317869","citation_count":0,"is_preprint":true}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":9770,"output_tokens":1364,"usd":0.024885,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7952,"output_tokens":1826,"usd":0.042705,"stage2_stop_reason":"end_turn"},"total_usd":0.06759,"stage1_batch_id":"msgbatch_0179Z2pxRYBsXi2xADneafRw","stage2_batch_id":"msgbatch_01LaY9ZaVdgjWArcZo9eqQE5","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2011,\n      \"finding\": \"ANKRD46 is a direct transcriptional target of miR-21; miR-21 binds the 3' UTR of ANKRD46 and suppresses its expression, as demonstrated by luciferase reporter assay and Western blot showing increased ANKRD46 protein upon miR-21 knockdown in breast cancer cells.\",\n      \"method\": \"Luciferase reporter assay with 3' UTR construct, Western blot, LNA-based miR-21 knockdown with microarray\",\n      \"journal\": \"Breast cancer research : BCR\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — luciferase reporter assay with 3' UTR validation and Western blot, single lab, two orthogonal methods\",\n      \"pmids\": [\"21219636\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2014,\n      \"finding\": \"Ankrd46 is a direct target of miR-451 in mouse luminal epithelium; miR-451 binds and represses Ankrd46, and this interaction is implicated in embryo implantation, as shown by dual-luciferase assay and loss-of-function/gain-of-function experiments in vivo.\",\n      \"method\": \"Dual-luciferase reporter assay, miR-451 inhibitor/sponge loss-of-function in vivo, qRT-PCR, microarray\",\n      \"journal\": \"Fertility and sterility\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — luciferase reporter validation plus in vivo loss-of-function, single lab, multiple methods\",\n      \"pmids\": [\"25542822\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2014,\n      \"finding\": \"ANKRD46 is a validated miR-21 target in hepatocellular carcinoma cells; uptake of anti-miR-21 induces ANKRD46 expression and inhibits cell growth, confirming miR-21-mediated repression of ANKRD46 in this context.\",\n      \"method\": \"Anti-miR-21 treatment with target gene expression readout (ANKRD46 induction), shRNA screen for uptake determinants\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — ANKRD46 induction used as a functional readout but no direct mechanistic experiments on ANKRD46 itself; single lab, single method for this gene\",\n      \"pmids\": [\"25550434\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"ANKRD46 expression is associated with the IL6-JAK-STAT3 signaling pathway in keloid and T2DM fibrotic contexts, and miR-21 regulates ANKRD46 expression within the fibrotic microenvironment; validated by Western blotting, qRT-PCR, IHC, and flow cytometry in mouse models.\",\n      \"method\": \"Western blotting, qRT-PCR, IHC, flow cytometry, mouse models, LASSO/SVM-RFE machine learning for biomarker identification\",\n      \"journal\": \"International journal of surgery (London, England)\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — functional association with IL6-JAK-STAT3 pathway reported with multiple experimental validation methods but mechanistic details are limited in the abstract; single lab\",\n      \"pmids\": [\"41346282\"],\n      \"is_preprint\": false\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"Circ_PRDM5 acts as a sponge for miR-25-3p, which in turn targets ANKRD46; circ_PRDM5 overexpression induces ANKRD46 upregulation via miR-25-3p sponging, and inhibition of miR-25-3p increases ANKRD46 levels and retards breast cancer progression, establishing ANKRD46 as a downstream effector of the circ_PRDM5/miR-25-3p axis.\",\n      \"method\": \"Dual-luciferase reporter assay, Western blot, MTT assay, EdU proliferation assay, transwell migration/invasion, xenograft tumor assay, qRT-PCR\",\n      \"journal\": \"Journal of biochemical and molecular toxicology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — luciferase reporter and functional cell assays confirm ANKRD46 as miR-25-3p target, but mechanistic role of ANKRD46 protein itself is not directly characterized; single lab\",\n      \"pmids\": [\"37485755\"],\n      \"is_preprint\": false\n    }\n  ],\n  \"current_model\": \"ANKRD46 is a direct target of multiple miRNAs (miR-21, miR-451, miR-25-3p), whose 3' UTR is bound and repressed by these miRNAs; it functions as a downstream effector in breast cancer cell growth suppression, embryo implantation, and fibrotic signaling (potentially via the IL6-JAK-STAT3 pathway), but its intrinsic molecular function and biochemical activity remain undefined.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"ANKRD46 is consistently identified as a 3' UTR-targeted effector gene regulated by oncogenic and tissue-context miRNAs, rather than through any defined intrinsic biochemical activity [#0, #1]. It is a direct target of miR-21, which binds its 3' UTR and represses its expression in breast cancer cells, such that miR-21 knockdown increases ANKRD46 protein [#0]. In mouse luminal epithelium, Ankrd46 is repressed by miR-451 in a manner implicated in embryo implantation [#1]. The intrinsic molecular function and biochemical activity of the ANKRD46 protein have not been characterized in the available corpus; all available evidence positions it as a downstream node of miRNA-mediated regulation across cancer and reproductive tissues.\",\n  \"teleology\": [\n    {\n      \"year\": 2011,\n      \"claim\": \"Established ANKRD46 as a bona fide direct target of an oncogenic miRNA, defining the first regulatory input into this otherwise uncharacterized gene.\",\n      \"evidence\": \"Luciferase 3' UTR reporter, Western blot, and LNA-based miR-21 knockdown in breast cancer cells\",\n      \"pmids\": [\"21219636\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No characterization of ANKRD46 protein function or biochemical activity\",\n        \"Whether ANKRD46 mediates downstream phenotypes of miR-21 was not tested\",\n        \"No subcellular localization or interaction data\"\n      ]\n    },\n    {\n      \"year\": 2014,\n      \"claim\": \"Extended ANKRD46 regulation beyond cancer to a developmental context by showing miR-451 represses it in luminal epithelium during embryo implantation.\",\n      \"evidence\": \"Dual-luciferase reporter assay with in vivo miR-451 loss-of-function (inhibitor/sponge) and qRT-PCR in mouse\",\n      \"pmids\": [\"25542822\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Mechanistic role of Ankrd46 protein in implantation not defined\",\n        \"Downstream effectors of Ankrd46 unknown\",\n        \"No demonstration of direct phenotypic rescue by Ankrd46 modulation\"\n      ]\n    },\n    {\n      \"year\": 2014,\n      \"claim\": \"Reinforced miR-21-mediated repression of ANKRD46 in a second cancer type, using ANKRD46 induction as a readout of anti-miR-21 activity.\",\n      \"evidence\": \"Anti-miR-21 treatment with ANKRD46 expression readout in hepatocellular carcinoma cells\",\n      \"pmids\": [\"25550434\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"ANKRD46 used only as a functional readout, no direct mechanistic experiments on the gene\",\n        \"Causal contribution of ANKRD46 to growth inhibition not isolated\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Placed ANKRD46 within the circ_PRDM5/miR-25-3p competing-endogenous-RNA axis in breast cancer, adding a third miRNA regulator.\",\n      \"evidence\": \"Dual-luciferase reporter, Western blot, proliferation/migration assays, and xenograft in breast cancer\",\n      \"pmids\": [\"37485755\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Mechanistic role of ANKRD46 protein itself not characterized\",\n        \"How ANKRD46 retards tumor progression at the molecular level is unknown\",\n        \"Direct binding of miR-25-3p to ANKRD46 versus indirect effects not fully separated\"\n      ]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Associated ANKRD46 expression with the IL6-JAK-STAT3 pathway in fibrotic tissue, hinting at a pathway context but without defining ANKRD46's molecular action.\",\n      \"evidence\": \"Western blot, qRT-PCR, IHC, flow cytometry, and machine-learning biomarker analysis in keloid/T2DM mouse models\",\n      \"pmids\": [\"41346282\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Association reported without mechanistic linkage of ANKRD46 to IL6-JAK-STAT3 signaling\",\n        \"Whether ANKRD46 acts upstream or downstream of the pathway is undetermined\",\n        \"No direct biochemical or interaction data\"\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The intrinsic molecular function, biochemical activity, subcellular localization, and physical interaction partners of the ANKRD46 protein remain undefined.\",\n      \"evidence\": \"No discovery in the corpus directly characterizes the ANKRD46 protein\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No enzymatic or binding activity assigned\",\n        \"No localization or complex membership established\",\n        \"Downstream effectors mediating its phenotypic roles unknown\"\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": []\n  }\n}","audit_flag":null,"evaluation":{"faith_supported":3,"faith_total":3,"faith_pct":100.0}}